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The map label for this gene is amiD [H]

Identifier: 189184417

GI number: 189184417

Start: 1538557

End: 1539654

Strand: Reverse

Name: amiD [H]

Synonym: OTT_1510

Alternate gene names: 189184417

Gene position: 1539654-1538557 (Counterclockwise)

Preceding gene: 189184423

Following gene: 189184416

Centisome position: 76.64

GC content: 32.51

Gene sequence:

>1098_bases
ATGCTTTATTTGCCACTATTGCAACTATTACAACATGTTATTGTTGATAATGCGTTAGTAGTTAATAAGTCCAATATTCA
TCAGAAAAGTCAATTACAGACTACAATATCAAGCCAGCATCATAAAATACAGCATACTCCTATAGAACAAGTAAATGTTT
TTAAACATGCGTTTCCTTCAGATAAAAATACTGGCATTTATGTTTCTCAATTTTCTGATTATGGAATTACAGGGTATGCA
ATAGATGAATTTCCTGAAAATGAAGACAACCGCAACCATTATACTCAACGAAAGCAAGATACTCCTATAAAGTACCTAAT
TATGCATTACACAGTAGGCAATTTTGCAAGTACCGCGCAAGAGTTTACTGTTAACAAACCTTTATTAGGTCAAGTTAGCT
CTCATATAGTAATTACTGAAGCAGAGGAAGGAAAAACACCAGCAGGAATTTTAATATCTTTTGTTCCAGAAAACAAAATT
GCTTATCATGCAGGAGTTAGTGCCTGGCAAAACGATACAAACTTGAATAGCATGTCAATAGGAATTGAGCATGTTAACAG
AGGATTTAATACAGTAAATGCAAAAAAGGAGTGGTTTTGCTATGATAAACATCAAATAGCAACTTCAGGTATAGTTAGTT
CTGGAATAATAAGGCGATATAACATAAAGCCTATTCATGTTTTAGGTCACTCAGATATCGCATATAATCGAAAGAGTGAT
CCTGGCCCTTTATTCCCATGGGGACAATTATATCATGAGTATAATGTTGGAGCATGGTTAACTGATGCAGAACTAAATTC
TCAATATATTAAAGAAAAATATAATCCTAAGATCTCTGATCCAGATAAAATAGATGATATTACTTTTTTAAAATTGCTAC
AAGAATATGGATATCACATTCCAGACAACGTTGATTTAAATAAAACAGATAATCCAAAGCAAACTGTTGCACTTTGTGCT
CCACTAATATTAGCTTTTAAGGCCCATTTTTCAGCTAATCAACATCCTGAACTTTGTGATAATGCTCAAATTACATCTAC
TGATAAATATTGGATTTGGGCGTTAACTGCGAAATATCAAAGTCAGATAAATCAATAA

Upstream 100 bases:

>100_bases
AAACTTTCAACTTTAAACAAGTGCTATACTGATTGCAAGCTTATAGTTAACAATGCTATACTCAGCTTATTATACCTGTT
CAACAATTATTAAATAAAAC

Downstream 100 bases:

>100_bases
TATTATGCTTAATAAAATTCTTAAATAAACACGAGTTTGAATGTAAGAAATAATATAAGACATAAGTAGAATAAGTATTA
CAACACCAGAACATGCTAAG

Product: putative N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MLYLPLLQLLQHVIVDNALVVNKSNIHQKSQLQTTISSQHHKIQHTPIEQVNVFKHAFPSDKNTGIYVSQFSDYGITGYA
IDEFPENEDNRNHYTQRKQDTPIKYLIMHYTVGNFASTAQEFTVNKPLLGQVSSHIVITEAEEGKTPAGILISFVPENKI
AYHAGVSAWQNDTNLNSMSIGIEHVNRGFNTVNAKKEWFCYDKHQIATSGIVSSGIIRRYNIKPIHVLGHSDIAYNRKSD
PGPLFPWGQLYHEYNVGAWLTDAELNSQYIKEKYNPKISDPDKIDDITFLKLLQEYGYHIPDNVDLNKTDNPKQTVALCA
PLILAFKAHFSANQHPELCDNAQITSTDKYWIWALTAKYQSQINQ

Sequences:

>Translated_365_residues
MLYLPLLQLLQHVIVDNALVVNKSNIHQKSQLQTTISSQHHKIQHTPIEQVNVFKHAFPSDKNTGIYVSQFSDYGITGYA
IDEFPENEDNRNHYTQRKQDTPIKYLIMHYTVGNFASTAQEFTVNKPLLGQVSSHIVITEAEEGKTPAGILISFVPENKI
AYHAGVSAWQNDTNLNSMSIGIEHVNRGFNTVNAKKEWFCYDKHQIATSGIVSSGIIRRYNIKPIHVLGHSDIAYNRKSD
PGPLFPWGQLYHEYNVGAWLTDAELNSQYIKEKYNPKISDPDKIDDITFLKLLQEYGYHIPDNVDLNKTDNPKQTVALCA
PLILAFKAHFSANQHPELCDNAQITSTDKYWIWALTAKYQSQINQ
>Mature_365_residues
MLYLPLLQLLQHVIVDNALVVNKSNIHQKSQLQTTISSQHHKIQHTPIEQVNVFKHAFPSDKNTGIYVSQFSDYGITGYA
IDEFPENEDNRNHYTQRKQDTPIKYLIMHYTVGNFASTAQEFTVNKPLLGQVSSHIVITEAEEGKTPAGILISFVPENKI
AYHAGVSAWQNDTNLNSMSIGIEHVNRGFNTVNAKKEWFCYDKHQIATSGIVSSGIIRRYNIKPIHVLGHSDIAYNRKSD
PGPLFPWGQLYHEYNVGAWLTDAELNSQYIKEKYNPKISDPDKIDDITFLKLLQEYGYHIPDNVDLNKTDNPKQTVALCA
PLILAFKAHFSANQHPELCDNAQITSTDKYWIWALTAKYQSQINQ

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=230, Percent_Identity=36.0869565217391, Blast_Score=131, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 41539; Mature: 41539

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYLPLLQLLQHVIVDNALVVNKSNIHQKSQLQTTISSQHHKIQHTPIEQVNVFKHAFPS
CCHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHCCCEECCCCHHHHHHHHHHCCC
DKNTGIYVSQFSDYGITGYAIDEFPENEDNRNHYTQRKQDTPIKYLIMHYTVGNFASTAQ
CCCCCEEEEECCCCCEEEEECCCCCCCCCCCCHHHHCCCCCCEEEEEEEEECCCHHHHHH
EFTVNKPLLGQVSSHIVITEAEEGKTPAGILISFVPENKIAYHAGVSAWQNDTNLNSMSI
HHCCCCCHHHCCCCCEEEEECCCCCCCCEEEEEECCCCCEEEECCCHHHCCCCCCCCEEE
GIEHVNRGFNTVNAKKEWFCYDKHQIATSGIVSSGIIRRYNIKPIHVLGHSDIAYNRKSD
EHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHCCEEEECCCEEEEECCCCEECCCCCC
PGPLFPWGQLYHEYNVGAWLTDAELNSQYIKEKYNPKISDPDKIDDITFLKLLQEYGYHI
CCCCCCHHHHHEECCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCC
PDNVDLNKTDNPKQTVALCAPLILAFKAHFSANQHPELCDNAQITSTDKYWIWALTAKYQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCEEEEEEEHHHH
SQINQ
HHCCC
>Mature Secondary Structure
MLYLPLLQLLQHVIVDNALVVNKSNIHQKSQLQTTISSQHHKIQHTPIEQVNVFKHAFPS
CCHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHCCCEECCCCHHHHHHHHHHCCC
DKNTGIYVSQFSDYGITGYAIDEFPENEDNRNHYTQRKQDTPIKYLIMHYTVGNFASTAQ
CCCCCEEEEECCCCCEEEEECCCCCCCCCCCCHHHHCCCCCCEEEEEEEEECCCHHHHHH
EFTVNKPLLGQVSSHIVITEAEEGKTPAGILISFVPENKIAYHAGVSAWQNDTNLNSMSI
HHCCCCCHHHCCCCCEEEEECCCCCCCCEEEEEECCCCCEEEECCCHHHCCCCCCCCEEE
GIEHVNRGFNTVNAKKEWFCYDKHQIATSGIVSSGIIRRYNIKPIHVLGHSDIAYNRKSD
EHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHCCEEEECCCEEEEECCCCEECCCCCC
PGPLFPWGQLYHEYNVGAWLTDAELNSQYIKEKYNPKISDPDKIDDITFLKLLQEYGYHI
CCCCCCHHHHHEECCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCC
PDNVDLNKTDNPKQTVALCAPLILAFKAHFSANQHPELCDNAQITSTDKYWIWALTAKYQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCEEEEEEEHHHH
SQINQ
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]