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The map label for this gene is rsmE [H]

Identifier: 189184337

GI number: 189184337

Start: 1464982

End: 1465773

Strand: Reverse

Name: rsmE [H]

Synonym: OTT_1430

Alternate gene names: 189184337

Gene position: 1465773-1464982 (Counterclockwise)

Preceding gene: 189184346

Following gene: 189184336

Centisome position: 72.96

GC content: 30.05

Gene sequence:

>792_bases
ATGAAACTTAAAAAATTACACAGAATATATAGCAGCATACCATTGAGTAAAGCTGGTACTATCAAACTATCAAGAGAGCA
AACTAACTACATAATCAATGTACTAAGATGTAAAAACAATGATAAAATTAGAGTATTTAATGAACTGAATGGAGAATATT
TAGCTATTATTCATCATACTAACAACAAACAAGTGTTATTGCAAATTATAAATCAAACTAAAGTAGTTGAGAAAGAAGTT
GATGAAAATAACTCACCTTTAGTTCTTGCTCAGTGTATTATTAAACCAGAAAGAATGGCCAGAGTAATTGATATGGCAAC
TCAAGCAGGAATAACAAACATAGTACCAATCATAAGCGAATATTCTCAACATCTAAAATTCAACATAGAAAAATATAATA
AAATCATCATAGAATCTTGCAGGCAATCAGAACGATTAACCATGCCTATTCTATCATCTCCAGTAACGTTATCACATTTT
TTAAGCCAAAGTAACTCAGGGTGCATATTATGTGCTAATGAACAGGAAAGAATACAACAAATACATCATATTCCAGCATC
AATACTTTCTAAAGCTTCAATTTTAATTGGGCCAGAGGGCGGTTTTTCTGAACAGGATCAAAGCATATTAAGGAAAGCAG
GAGGATTAATAAAATCGATAAGCATTGGAAGAAATGTTTTAAGATCTGAAACTGCTGCAATATATCTTTTATCGCAGTTA
CAATTATTAAAAACTATGCACGTACATAAAGGCAATTACGAAGATATTAATATTAATAAAGTTTTCAATTGA

Upstream 100 bases:

>100_bases
ATTTTAATTGCTTTGCAGATAATAATTTAAAAAAATAAAAATGTCAAATATCGATTGATTAATTTATATTATCAATATTT
AATACATTCCAAATTAGAAT

Downstream 100 bases:

>100_bases
TTCGGTATTTCAAAAACTATATATACCAAAGAAATCAGATGTATGATGGATGTTGCTTTTTTCAAACTTCACTTATATCA
TTTACAATAAACTTATTACA

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MKLKKLHRIYSSIPLSKAGTIKLSREQTNYIINVLRCKNNDKIRVFNELNGEYLAIIHHTNNKQVLLQIINQTKVVEKEV
DENNSPLVLAQCIIKPERMARVIDMATQAGITNIVPIISEYSQHLKFNIEKYNKIIIESCRQSERLTMPILSSPVTLSHF
LSQSNSGCILCANEQERIQQIHHIPASILSKASILIGPEGGFSEQDQSILRKAGGLIKSISIGRNVLRSETAAIYLLSQL
QLLKTMHVHKGNYEDININKVFN

Sequences:

>Translated_263_residues
MKLKKLHRIYSSIPLSKAGTIKLSREQTNYIINVLRCKNNDKIRVFNELNGEYLAIIHHTNNKQVLLQIINQTKVVEKEV
DENNSPLVLAQCIIKPERMARVIDMATQAGITNIVPIISEYSQHLKFNIEKYNKIIIESCRQSERLTMPILSSPVTLSHF
LSQSNSGCILCANEQERIQQIHHIPASILSKASILIGPEGGFSEQDQSILRKAGGLIKSISIGRNVLRSETAAIYLLSQL
QLLKTMHVHKGNYEDININKVFN
>Mature_263_residues
MKLKKLHRIYSSIPLSKAGTIKLSREQTNYIINVLRCKNNDKIRVFNELNGEYLAIIHHTNNKQVLLQIINQTKVVEKEV
DENNSPLVLAQCIIKPERMARVIDMATQAGITNIVPIISEYSQHLKFNIEKYNKIIIESCRQSERLTMPILSSPVTLSHF
LSQSNSGCILCANEQERIQQIHHIPASILSKASILIGPEGGFSEQDQSILRKAGGLIKSISIGRNVLRSETAAIYLLSQL
QLLKTMHVHKGNYEDININKVFN

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=247, Percent_Identity=28.7449392712551, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 29733; Mature: 29733

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKKLHRIYSSIPLSKAGTIKLSREQTNYIINVLRCKNNDKIRVFNELNGEYLAIIHHT
CCHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEEECCCCCEEEEEECCCCEEEEEEEC
NNKQVLLQIINQTKVVEKEVDENNSPLVLAQCIIKPERMARVIDMATQAGITNIVPIISE
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHH
YSQHLKFNIEKYNKIIIESCRQSERLTMPILSSPVTLSHFLSQSNSGCILCANEQERIQQ
HHHHHEECHHHHHHHHHHHHCCCCCEECCHHCCCHHHHHHHCCCCCCEEEECCCHHHHHH
IHHIPASILSKASILIGPEGGFSEQDQSILRKAGGLIKSISIGRNVLRSETAAIYLLSQL
HHHCHHHHHCCCEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
QLLKTMHVHKGNYEDININKVFN
HHHHHHHHCCCCCCEEEEEECCC
>Mature Secondary Structure
MKLKKLHRIYSSIPLSKAGTIKLSREQTNYIINVLRCKNNDKIRVFNELNGEYLAIIHHT
CCHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEEECCCCCEEEEEECCCCEEEEEEEC
NNKQVLLQIINQTKVVEKEVDENNSPLVLAQCIIKPERMARVIDMATQAGITNIVPIISE
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHH
YSQHLKFNIEKYNKIIIESCRQSERLTMPILSSPVTLSHFLSQSNSGCILCANEQERIQQ
HHHHHEECHHHHHHHHHHHHCCCCCEECCHHCCCHHHHHHHCCCCCCEEEECCCHHHHHH
IHHIPASILSKASILIGPEGGFSEQDQSILRKAGGLIKSISIGRNVLRSETAAIYLLSQL
HHHCHHHHHCCCEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
QLLKTMHVHKGNYEDININKVFN
HHHHHHHHCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]