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The map label for this gene is uvrD [H]

Identifier: 189183408

GI number: 189183408

Start: 517545

End: 519695

Strand: Direct

Name: uvrD [H]

Synonym: OTT_0501

Alternate gene names: 189183408

Gene position: 517545-519695 (Clockwise)

Preceding gene: 189183406

Following gene: 189183412

Centisome position: 25.76

GC content: 29.89

Gene sequence:

>2151_bases
ATGAGTCAGTATTTATTAGATCTAAATCCAGAACAACGAGAAGCAGTAATAACTACTAATGGTCCATTGTTAGTATTAGC
TGGGGCTGGCACAGGTAAAACAAGAGTGATAACTTATCGTATTGCTTATATTATTGACCAATTTATGGCATTACCAGAAG
AAATTCTTGCAGTAACATTTACTAATAAAGCCGCAACAGAAATGCAATCGCGTATCGCAGAAATAACTAACAATACTACA
GGTATATACATTGGAACTTTTCATGCTATTGCTACTAAAATTCTTAAAGCACATGCTGAGTTGGTAGGGTTAACATCATC
ATTTTCAATAATAGACCAATCTGAACAAGTCAGAGTTATTAAAAATATTTGTGAAACACATGGCATTTCAACAAAAAATT
ATTCCCCAAAAGATATTTGCGAAATAATAAGTAGATGGAAAGATCTTGGTGAGCAGCATAAGATAGATTGCCATACTCCT
ATTTATCAAGTTGCACAAAAAATCTACCAACATTATCAAACAGAATTACAACAATCAAATGTAGTTGATTTTGGAGATTT
ATTATTATACAACATGAAAATTTTCAGTGAATATCCGGATGTTTTAAATTTTTATCAAACAAAATTTAAGTATGTTTTTA
TTGATGAATATCAAGATACTAATAATGTTCAGTATTTTTGGGCTAAAGCTTTAGTCAATTTGCATGAAAATTTATGTTGT
GTAGGTGATGACGACCAATCAATTTATAGTTGGAGAGGAGCAAACATAGAAAATATTTTAAAGTTTAAAGACGACTTCAA
AAACGCTAAGTTAATTAAGCTTGAGCAAAATTATAGATCAACTAATAGCATTTTAACAGCAGCTAGTAGTATCATTTGCA
ACAATAAATATCGTCATGATAAAAAACTATGGACTAATAAACAATCTGATCAACCAATAAGGATTGTATATTGTTATAAT
GATCGTGAAGAAGCACGGTTTATTAGCTCTGAAATTAAAAAATTAATAACTAAATCACCTAATCTTTCCATTGCTGTACT
AGTTAGATCTAGCTCCCAAGCTCATGTTTTAGAAGAGTCATTTTTTAGCAATAATATAAAATATCAAGTAATTGGTGGGT
TTAGATTTTTTGATAGAATGGAAATTGCAGATATGATAGCATATTTGCGAGTACTAGTAAATCACAATGATAATTTAGCA
TTAACTAGAATTATTAATGTACCTAAAAGATCTATTGGTCCAGCAACATTACATACTATTAAGGAGTATGCTCAGCAACA
TGAAAGTTCAATGTTTACTGCGATTGAGCAGATATTAAGTAAAAAACTTTTAAAACCTCAGCTAGAGTTAACATTACAAA
ATTTAATTAACAAATTTAAAGAATGGCGTTCTCTATATAAAGCTGGTTATACTCCTGCTAAGGTAACTGAAATTATTTGC
GATGACTCTAATTATTTACAAATGTTAAAAGATGATAAATCTTCTCAGAGCGACAGAAGAATAGAAAATATTCAAGAAAT
GATTAGAGCAATGCTAGATTATAGTAGTATCGAAGAGTTTTTAGAACATGCAAGTTTATTTTTGGAACGAGATAATGGTA
TTAACGCTAAAGATAATAGCGTAGTTAGCTTAATGACTTTACATGCATCTAAGGGTTTAGAATTTGATGTAGTATTTTTA
CCTGGATGGGAAGAAGGAGTTTTCCCTCATCAAAAATCACTTATTGTTGAAGATCAAAGAGCTTTAGAAGAAGAAAGAAG
GATAGCTTATGTTGGAATTACCAGAGCTAAACAAAGTTTATACATTAGTTATACTGATCAAAGAAGAATATTTAATGAAT
TTGTAAAACTACAACCGTCAAGATTTTTATTAGATATACCTGATAGTATATCATTATCTACAACTTCAATAATACATTAT
AGCAGTGCTGCTAATAACAACTATTTTCAAAAATTATCATCAGCAGCAGTGCCTAATTCCCAATCTAAGGCTTCAAGCAA
ATTTTTTACAGGTGCAAAAGTTCTGCATAAACAATTTGGGCAAGGCACAGTAATAAGGATTATGAATGATAACATTGAAG
TAGCATTTTACGTTGGTGGAATAAAAACTATAAAACAGCAATTCTTAACTATTAACGCTAGATTAAGATAA

Upstream 100 bases:

>100_bases
AGTCTAATATCCTTTTGCAGAAATTCTGTTAATAATTTTTAATTACATTCATTCAAGTATGTGTAACAATATGTAAAAAT
TTATGTTAAATTAAGAAAAT

Downstream 100 bases:

>100_bases
GTTGAATGAAGAAAAAGAAGAGGAGATAAGGTAGGGATTAAGTGTAGAGATAGAATAACTAGCAAGAGAAGCAATTATAT
GAACAAAGAAATTAAGAAGA

Product: DNA helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 716; Mature: 715

Protein sequence:

>716_residues
MSQYLLDLNPEQREAVITTNGPLLVLAGAGTGKTRVITYRIAYIIDQFMALPEEILAVTFTNKAATEMQSRIAEITNNTT
GIYIGTFHAIATKILKAHAELVGLTSSFSIIDQSEQVRVIKNICETHGISTKNYSPKDICEIISRWKDLGEQHKIDCHTP
IYQVAQKIYQHYQTELQQSNVVDFGDLLLYNMKIFSEYPDVLNFYQTKFKYVFIDEYQDTNNVQYFWAKALVNLHENLCC
VGDDDQSIYSWRGANIENILKFKDDFKNAKLIKLEQNYRSTNSILTAASSIICNNKYRHDKKLWTNKQSDQPIRIVYCYN
DREEARFISSEIKKLITKSPNLSIAVLVRSSSQAHVLEESFFSNNIKYQVIGGFRFFDRMEIADMIAYLRVLVNHNDNLA
LTRIINVPKRSIGPATLHTIKEYAQQHESSMFTAIEQILSKKLLKPQLELTLQNLINKFKEWRSLYKAGYTPAKVTEIIC
DDSNYLQMLKDDKSSQSDRRIENIQEMIRAMLDYSSIEEFLEHASLFLERDNGINAKDNSVVSLMTLHASKGLEFDVVFL
PGWEEGVFPHQKSLIVEDQRALEEERRIAYVGITRAKQSLYISYTDQRRIFNEFVKLQPSRFLLDIPDSISLSTTSIIHY
SSAANNNYFQKLSSAAVPNSQSKASSKFFTGAKVLHKQFGQGTVIRIMNDNIEVAFYVGGIKTIKQQFLTINARLR

Sequences:

>Translated_716_residues
MSQYLLDLNPEQREAVITTNGPLLVLAGAGTGKTRVITYRIAYIIDQFMALPEEILAVTFTNKAATEMQSRIAEITNNTT
GIYIGTFHAIATKILKAHAELVGLTSSFSIIDQSEQVRVIKNICETHGISTKNYSPKDICEIISRWKDLGEQHKIDCHTP
IYQVAQKIYQHYQTELQQSNVVDFGDLLLYNMKIFSEYPDVLNFYQTKFKYVFIDEYQDTNNVQYFWAKALVNLHENLCC
VGDDDQSIYSWRGANIENILKFKDDFKNAKLIKLEQNYRSTNSILTAASSIICNNKYRHDKKLWTNKQSDQPIRIVYCYN
DREEARFISSEIKKLITKSPNLSIAVLVRSSSQAHVLEESFFSNNIKYQVIGGFRFFDRMEIADMIAYLRVLVNHNDNLA
LTRIINVPKRSIGPATLHTIKEYAQQHESSMFTAIEQILSKKLLKPQLELTLQNLINKFKEWRSLYKAGYTPAKVTEIIC
DDSNYLQMLKDDKSSQSDRRIENIQEMIRAMLDYSSIEEFLEHASLFLERDNGINAKDNSVVSLMTLHASKGLEFDVVFL
PGWEEGVFPHQKSLIVEDQRALEEERRIAYVGITRAKQSLYISYTDQRRIFNEFVKLQPSRFLLDIPDSISLSTTSIIHY
SSAANNNYFQKLSSAAVPNSQSKASSKFFTGAKVLHKQFGQGTVIRIMNDNIEVAFYVGGIKTIKQQFLTINARLR
>Mature_715_residues
SQYLLDLNPEQREAVITTNGPLLVLAGAGTGKTRVITYRIAYIIDQFMALPEEILAVTFTNKAATEMQSRIAEITNNTTG
IYIGTFHAIATKILKAHAELVGLTSSFSIIDQSEQVRVIKNICETHGISTKNYSPKDICEIISRWKDLGEQHKIDCHTPI
YQVAQKIYQHYQTELQQSNVVDFGDLLLYNMKIFSEYPDVLNFYQTKFKYVFIDEYQDTNNVQYFWAKALVNLHENLCCV
GDDDQSIYSWRGANIENILKFKDDFKNAKLIKLEQNYRSTNSILTAASSIICNNKYRHDKKLWTNKQSDQPIRIVYCYND
REEARFISSEIKKLITKSPNLSIAVLVRSSSQAHVLEESFFSNNIKYQVIGGFRFFDRMEIADMIAYLRVLVNHNDNLAL
TRIINVPKRSIGPATLHTIKEYAQQHESSMFTAIEQILSKKLLKPQLELTLQNLINKFKEWRSLYKAGYTPAKVTEIICD
DSNYLQMLKDDKSSQSDRRIENIQEMIRAMLDYSSIEEFLEHASLFLERDNGINAKDNSVVSLMTLHASKGLEFDVVFLP
GWEEGVFPHQKSLIVEDQRALEEERRIAYVGITRAKQSLYISYTDQRRIFNEFVKLQPSRFLLDIPDSISLSTTSIIHYS
SAANNNYFQKLSSAAVPNSQSKASSKFFTGAKVLHKQFGQGTVIRIMNDNIEVAFYVGGIKTIKQQFLTINARLR

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=723, Percent_Identity=38.0359612724758, Blast_Score=499, Evalue=1e-142,
Organism=Escherichia coli, GI48994965, Length=650, Percent_Identity=37.2307692307692, Blast_Score=393, Evalue=1e-110,
Organism=Escherichia coli, GI1787196, Length=337, Percent_Identity=26.1127596439169, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322369, Length=754, Percent_Identity=29.0450928381963, Blast_Score=224, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6324477, Length=685, Percent_Identity=24.5255474452555, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 82198; Mature: 82067

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQYLLDLNPEQREAVITTNGPLLVLAGAGTGKTRVITYRIAYIIDQFMALPEEILAVTF
CCCEEECCCCCCCCEEEEECCCEEEEEECCCCCEEEEEHHHHHHHHHHHHCCHHHEEEEE
TNKAATEMQSRIAEITNNTTGIYIGTFHAIATKILKAHAELVGLTSSFSIIDQSEQVRVI
CCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
KNICETHGISTKNYSPKDICEIISRWKDLGEQHKIDCHTPIYQVAQKIYQHYQTELQQSN
HHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
VVDFGDLLLYNMKIFSEYPDVLNFYQTKFKYVFIDEYQDTNNVQYFWAKALVNLHENLCC
CCCHHHHHHHHHHHHHHCHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHCCEEE
VGDDDQSIYSWRGANIENILKFKDDFKNAKLIKLEQNYRSTNSILTAASSIICNNKYRHD
ECCCCCCHHEECCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCC
KKLWTNKQSDQPIRIVYCYNDREEARFISSEIKKLITKSPNLSIAVLVRSSSQAHVLEES
HHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHH
FFSNNIKYQVIGGFRFFDRMEIADMIAYLRVLVNHNDNLALTRIINVPKRSIGPATLHTI
HHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHH
KEYAQQHESSMFTAIEQILSKKLLKPQLELTLQNLINKFKEWRSLYKAGYTPAKVTEIIC
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEE
DDSNYLQMLKDDKSSQSDRRIENIQEMIRAMLDYSSIEEFLEHASLFLERDNGINAKDNS
CCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHEEECCCCCCCCCCC
VVSLMTLHASKGLEFDVVFLPGWEEGVFPHQKSLIVEDQRALEEERRIAYVGITRAKQSL
EEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCEEEHHHHHHHHHCCEEEEEEEECCCEE
YISYTDQRRIFNEFVKLQPSRFLLDIPDSISLSTTSIIHYSSAANNNYFQKLSSAAVPNS
EEEECHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCC
QSKASSKFFTGAKVLHKQFGQGTVIRIMNDNIEVAFYVGGIKTIKQQFLTINARLR
CCHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEECCHHHHHHHHHEEEEECC
>Mature Secondary Structure 
SQYLLDLNPEQREAVITTNGPLLVLAGAGTGKTRVITYRIAYIIDQFMALPEEILAVTF
CCEEECCCCCCCCEEEEECCCEEEEEECCCCCEEEEEHHHHHHHHHHHHCCHHHEEEEE
TNKAATEMQSRIAEITNNTTGIYIGTFHAIATKILKAHAELVGLTSSFSIIDQSEQVRVI
CCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
KNICETHGISTKNYSPKDICEIISRWKDLGEQHKIDCHTPIYQVAQKIYQHYQTELQQSN
HHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
VVDFGDLLLYNMKIFSEYPDVLNFYQTKFKYVFIDEYQDTNNVQYFWAKALVNLHENLCC
CCCHHHHHHHHHHHHHHCHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHCCEEE
VGDDDQSIYSWRGANIENILKFKDDFKNAKLIKLEQNYRSTNSILTAASSIICNNKYRHD
ECCCCCCHHEECCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCC
KKLWTNKQSDQPIRIVYCYNDREEARFISSEIKKLITKSPNLSIAVLVRSSSQAHVLEES
HHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHH
FFSNNIKYQVIGGFRFFDRMEIADMIAYLRVLVNHNDNLALTRIINVPKRSIGPATLHTI
HHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHH
KEYAQQHESSMFTAIEQILSKKLLKPQLELTLQNLINKFKEWRSLYKAGYTPAKVTEIIC
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEE
DDSNYLQMLKDDKSSQSDRRIENIQEMIRAMLDYSSIEEFLEHASLFLERDNGINAKDNS
CCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHEEECCCCCCCCCCC
VVSLMTLHASKGLEFDVVFLPGWEEGVFPHQKSLIVEDQRALEEERRIAYVGITRAKQSL
EEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCEEEHHHHHHHHHCCEEEEEEEECCCEE
YISYTDQRRIFNEFVKLQPSRFLLDIPDSISLSTTSIIHYSSAANNNYFQKLSSAAVPNS
EEEECHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCC
QSKASSKFFTGAKVLHKQFGQGTVIRIMNDNIEVAFYVGGIKTIKQQFLTINARLR
CCHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEECCHHHHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA