Click here to switch to the map view.

The map label for this gene is lepB [C]

Identifier: 189026149

GI number: 189026149

Start: 1007735

End: 1009273

Strand: Reverse

Name: lepB [C]

Synonym: TPASS_0926

Alternate gene names: 189026149

Gene position: 1009273-1007735 (Counterclockwise)

Preceding gene: 189026154

Following gene: 189026146

Centisome position: 88.57

GC content: 56.27

Gene sequence:

>1539_bases
ATGGTGCTGACTCTGTGTGTCTGTGCGCTGTTGTTTTGCCTGAGGAGGAAGTACGTACATCTCTTTTTTCCTCGTGGGGT
TTCGGTGCACACGCCCCCTGCGTCTTCGGACGTGCGGAGTGTGTTGCCGGATATGCCAGTGAGAAGGAGGCGAGGAATCT
TTGTCGTACTCGAATGGGTTGACGCGCTCACCCAGGCTGCGTGTTTCATGCTTTTGGTGAATTTGTTCGCGTTCCAGTTG
TACGTTATCCCGAGCGAATCGATGGTCCCCAGCTTTATGGTCGGCGATAGACTCCTCGTGTTCAAGACCGCCTCAGGGCC
TGTATTCCCGCTTTCTTCGTTTCGTTTGCCACGCTGGCGTACCTACAAGCGCGGAGACATCGTCGTTTTTTCCAATCCTC
ATTACCCTGACACTCCGCAGGATAAGCTCCGCGCCTTTTTAGCCCAATTAGTGTACATGCTCACCTTTACGCGCAAGAAC
ATTAATGTGGATCCTGTCACCGGTGCGCCGAAAGCTGATCCTCTCGTCAAACGCATTGTTGCTCTGCCAGGGGAAAAAGT
TATGCTCGTTGACGGTGTGCTCTATACGAAGACCAGGCATGATGCGCACTTCAAGCCTGTCGCACAAGACCGTACGTACG
CCACGTGGGATTTGAATGCGTTGCCCGCACGCGATTTGGCGCGTGTTCAACGGGTCATATTTAATGCTGAGGAGCTCGCC
GCCATCCATCTGGTAGAGCGCCTGCGCGCCCAGGTGGATTTTCGCGATTTAGCAGAGAAAACGCGCGCGTTGGTTGCCCA
AGCGCACGCGTACGCGGGGGCGGCGTCACGCACCCGACAGGGCATTGGCGTGGCGCAACCGATAACGCACACATCTGACA
TTCCTGCTTTACCTCTGTTTGAAAAAGAAATGCGCGGGGCGCGGGAGATCACACAGCTCTTCGCCACCGTTGCAGACGTT
GCCACGCATATCCGCGACACCTCCCAGGGGTTCGCCCACTTCGCTCACTTTGTGCAAAGCTGGATCCCATTTTGGGGGCA
AGGAACGTATGGCTTGGACACGGGACAGGAAGGTCCGTCCCTGCACCGCGCAGGCCTCTCGCTCTACCAGATAAGATTTG
CGCAGCTGAACGCGTTGGTGAAGTACACGTTCGCCCAGCTAGTGGTAAAAGGCCTCCAGGTGACAGCACACCGAACGTCG
GAGGCTGGGCAGGACGAAACGCTCACTACACTTTTGCAGGACGCGGCCCGGTACATCTTTTTCCTGGGTGCGGCGCGTGG
ATTCAACATGGACGAATTCCCCGCTGGCGCCGAGCAGTACCTTCCAGAACACAACTACTTCATGATGGGAGACAACCGAT
TGAACTCTACTGATATGCGCCACGCGTACACCGAACACCTCGAGGCAATCGACGCGCACGACCCGTTCCCTATTTTCTTT
AGCTCCAATGTTGCGCCCAAGTACATTCCCGATAGCCACATCCTCGGTGTGGCGTCGTTCCGATTCTGGCCGCCCTCCCG
CATAGGCACCCCACAATAG

Upstream 100 bases:

>100_bases
AATACACACCCTTTGTGCTGATGGCGTGCTTTGTCCTCTCCCGCGCGTATGCGCTACGGTGGCGATGCCGTGGTTAGATT
CCCTTTTGGGGATGAGTTGG

Downstream 100 bases:

>100_bases
GCTTAGGGGGAGCGGAGAGAAAGCTAAGAAAGGCGGAACCCGCCCAGGCGCGCAGCAGGCAGAAAGCCCGTACCCTCAGA
CAGGTCCCGTTACACAACAA

Product: signal peptidase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MVLTLCVCALLFCLRRKYVHLFFPRGVSVHTPPASSDVRSVLPDMPVRRRRGIFVVLEWVDALTQAACFMLLVNLFAFQL
YVIPSESMVPSFMVGDRLLVFKTASGPVFPLSSFRLPRWRTYKRGDIVVFSNPHYPDTPQDKLRAFLAQLVYMLTFTRKN
INVDPVTGAPKADPLVKRIVALPGEKVMLVDGVLYTKTRHDAHFKPVAQDRTYATWDLNALPARDLARVQRVIFNAEELA
AIHLVERLRAQVDFRDLAEKTRALVAQAHAYAGAASRTRQGIGVAQPITHTSDIPALPLFEKEMRGAREITQLFATVADV
ATHIRDTSQGFAHFAHFVQSWIPFWGQGTYGLDTGQEGPSLHRAGLSLYQIRFAQLNALVKYTFAQLVVKGLQVTAHRTS
EAGQDETLTTLLQDAARYIFFLGAARGFNMDEFPAGAEQYLPEHNYFMMGDNRLNSTDMRHAYTEHLEAIDAHDPFPIFF
SSNVAPKYIPDSHILGVASFRFWPPSRIGTPQ

Sequences:

>Translated_512_residues
MVLTLCVCALLFCLRRKYVHLFFPRGVSVHTPPASSDVRSVLPDMPVRRRRGIFVVLEWVDALTQAACFMLLVNLFAFQL
YVIPSESMVPSFMVGDRLLVFKTASGPVFPLSSFRLPRWRTYKRGDIVVFSNPHYPDTPQDKLRAFLAQLVYMLTFTRKN
INVDPVTGAPKADPLVKRIVALPGEKVMLVDGVLYTKTRHDAHFKPVAQDRTYATWDLNALPARDLARVQRVIFNAEELA
AIHLVERLRAQVDFRDLAEKTRALVAQAHAYAGAASRTRQGIGVAQPITHTSDIPALPLFEKEMRGAREITQLFATVADV
ATHIRDTSQGFAHFAHFVQSWIPFWGQGTYGLDTGQEGPSLHRAGLSLYQIRFAQLNALVKYTFAQLVVKGLQVTAHRTS
EAGQDETLTTLLQDAARYIFFLGAARGFNMDEFPAGAEQYLPEHNYFMMGDNRLNSTDMRHAYTEHLEAIDAHDPFPIFF
SSNVAPKYIPDSHILGVASFRFWPPSRIGTPQ
>Mature_512_residues
MVLTLCVCALLFCLRRKYVHLFFPRGVSVHTPPASSDVRSVLPDMPVRRRRGIFVVLEWVDALTQAACFMLLVNLFAFQL
YVIPSESMVPSFMVGDRLLVFKTASGPVFPLSSFRLPRWRTYKRGDIVVFSNPHYPDTPQDKLRAFLAQLVYMLTFTRKN
INVDPVTGAPKADPLVKRIVALPGEKVMLVDGVLYTKTRHDAHFKPVAQDRTYATWDLNALPARDLARVQRVIFNAEELA
AIHLVERLRAQVDFRDLAEKTRALVAQAHAYAGAASRTRQGIGVAQPITHTSDIPALPLFEKEMRGAREITQLFATVADV
ATHIRDTSQGFAHFAHFVQSWIPFWGQGTYGLDTGQEGPSLHRAGLSLYQIRFAQLNALVKYTFAQLVVKGLQVTAHRTS
EAGQDETLTTLLQDAARYIFFLGAARGFNMDEFPAGAEQYLPEHNYFMMGDNRLNSTDMRHAYTEHLEAIDAHDPFPIFF
SSNVAPKYIPDSHILGVASFRFWPPSRIGTPQ

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.4.21.89

Molecular weight: Translated: 57620; Mature: 57620

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS00760 SPASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLTLCVCALLFCLRRKYVHLFFPRGVSVHTPPASSDVRSVLPDMPVRRRRGIFVVLEWV
CHHHHHHHHHHHHHHHHHEEEECCCCCEEECCCCCHHHHHHCCCCCCHHHCCEEEEHHHH
DALTQAACFMLLVNLFAFQLYVIPSESMVPSFMVGDRLLVFKTASGPVFPLSSFRLPRWR
HHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHCCEEEEEEECCCCCCCCCCCCCCCCC
TYKRGDIVVFSNPHYPDTPQDKLRAFLAQLVYMLTFTRKNINVDPVTGAPKADPLVKRIV
CCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
ALPGEKVMLVDGVLYTKTRHDAHFKPVAQDRTYATWDLNALPARDLARVQRVIFNAEELA
HCCCCEEEEECCEEEECCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCHHHHH
AIHLVERLRAQVDFRDLAEKTRALVAQAHAYAGAASRTRQGIGVAQPITHTSDIPALPLF
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCCH
EKEMRGAREITQLFATVADVATHIRDTSQGFAHFAHFVQSWIPFWGQGTYGLDTGQEGPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
LHRAGLSLYQIRFAQLNALVKYTFAQLVVKGLQVTAHRTSEAGQDETLTTLLQDAARYIF
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHH
FLGAARGFNMDEFPAGAEQYLPEHNYFMMGDNRLNSTDMRHAYTEHLEAIDAHDPFPIFF
HHHCCCCCCCCCCCCCHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEE
SSNVAPKYIPDSHILGVASFRFWPPSRIGTPQ
ECCCCCCCCCCCCEEEEHEEECCCCCCCCCCC
>Mature Secondary Structure
MVLTLCVCALLFCLRRKYVHLFFPRGVSVHTPPASSDVRSVLPDMPVRRRRGIFVVLEWV
CHHHHHHHHHHHHHHHHHEEEECCCCCEEECCCCCHHHHHHCCCCCCHHHCCEEEEHHHH
DALTQAACFMLLVNLFAFQLYVIPSESMVPSFMVGDRLLVFKTASGPVFPLSSFRLPRWR
HHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHCCEEEEEEECCCCCCCCCCCCCCCCC
TYKRGDIVVFSNPHYPDTPQDKLRAFLAQLVYMLTFTRKNINVDPVTGAPKADPLVKRIV
CCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
ALPGEKVMLVDGVLYTKTRHDAHFKPVAQDRTYATWDLNALPARDLARVQRVIFNAEELA
HCCCCEEEEECCEEEECCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCHHHHH
AIHLVERLRAQVDFRDLAEKTRALVAQAHAYAGAASRTRQGIGVAQPITHTSDIPALPLF
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCCH
EKEMRGAREITQLFATVADVATHIRDTSQGFAHFAHFVQSWIPFWGQGTYGLDTGQEGPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
LHRAGLSLYQIRFAQLNALVKYTFAQLVVKGLQVTAHRTSEAGQDETLTTLLQDAARYIF
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHH
FLGAARGFNMDEFPAGAEQYLPEHNYFMMGDNRLNSTDMRHAYTEHLEAIDAHDPFPIFF
HHHCCCCCCCCCCCCCHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEE
SSNVAPKYIPDSHILGVASFRFWPPSRIGTPQ
ECCCCCCCCCCCCEEEEHEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Phospholipids; Triton X-100 [C]

Metal ions: NA

Kcat value (1/min): 523.8 [C]

Specific activity: NA

Km value (mM): 0.52 {Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile}} 0.33 {Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile}} 1 {Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile-CONH2}} 0.032 {pro-OmpA-nuclease} 0.0165 {pro-OmpA-nuclease} [C]

Substrates: NA

Specific reaction: NA

General reaction: Peptide bond hydrolysis [C]

Inhibitor: 1-Ethyl -3-(3-dimethyl aminopropyl) carbodiimide; beta-Lactams; Bromosuccinimide; Carboxyphenanthroline; Cholate; Cu2+; Deoxycholate; Diethyl dicarbonate; Dinitrophenol; Hg2+; Mg2+ most serine peptidases; NEM Cys; Phenyl glyoxal; SDS; Sodiumchloride [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA