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The map label for this gene is truB

Identifier: 189026112

GI number: 189026112

Start: 967759

End: 968859

Strand: Reverse

Name: truB

Synonym: TPASS_0889

Alternate gene names: 189026112

Gene position: 968859-967759 (Counterclockwise)

Preceding gene: 189026113

Following gene: 189026111

Centisome position: 85.03

GC content: 56.4

Gene sequence:

>1101_bases
GTGTGCAGACTGAGCATGCCTGACGCTATTGTTCCTTTCGCAAAGGTTTCCGGTCTTACGAGTTTTGCGGCACTGGCACA
GGTCAGGCGTCTTCTGGGAGTAAAAAAGGTAGGGCATACGGGGACGCTTGATCGCTTTGCTGATGGGCTGCTGTTGCTTT
TGGTAGGGGGCTTTACCAAACTCGCGCCGGTGATGACTCGCTTGGAAAAGAGTTACGAGGCTCGTATCCAGTTTGGGGTA
CAAACAGACACTCTAGATCCGGAGGGGGCTGTCGTGCGGTGCTCCTTGTTCCCAACATTTGCGCGCGTGCGTGCGGCGCT
GCCTCACTTCACTGGGAGTATTGATCAGGTGCCGCCTGAATATTCGGCGCTAAAATTCGGAGGTGTGCGTGCGTCCGACC
GGGTGCGGCGTGGGGAAGCAGTGTGCATGAAGGCTCGGCGTGTGTTCGTCTTTGACTTGCAGGTACTAGGTTGCGAGGCG
GATCTGGGTGAATTCAAAAAGACGCAGGCGGGGAGGGGGGCTGCGATTGCTGATCTTGATCTGACGCGCGTGCGTGCTGT
AACGCTGTACGTACGTTGTTCGGCAGGCTTCTACGTGCGTGCACTTGCGCGCGACATAGCAGCCGCTTGCGGCTCTTGCG
CGTATGTTTCACATTTACGGAGAACACGCATTGGACCCTTTGATCTTGCACAGGCGGCGGGTGTGTCTCGTCTCGGGTCC
TGGACATGGGGCAAAGAGCGTGCGTCTTGCGGTGCCGCTTGCTTTGATGTGGGTGCTCCACCGCCTCCGTCCTCAGGCGG
CGTGGCGACCGATTCAGTCTCTTTCGGGTGTGAAGACTTGACGGTGCGTGAGATTAAGCAGGCAGTGGTTAGCTGTGATG
TGGACTTTGCGAATCGTATTGGACTTACGGCGTGTTCAGTGCATGCGCAGTATGCGTCGCGGTTTTTGCATGGGGAAAGA
ATACGCGCATGTTGGTTCCAGTCGTTTGGTACGCGAAGGCCCGGGGAACGTGCGTTGGTGTTTTCCGAGGGTCGCTGTCT
TGGATTAATAAGGAAAGCGGCGAATGGGTTTTCGTATGATGCGGTATTTTGTACGGAGTGA

Upstream 100 bases:

>100_bases
CTCCGTCTGCGGCAGTGTCCGCGTCTTAGCTTTGTGTATGACGAGAGTGTGAGGGATGGATTTTCTCTTTCGAGAAAAAT
AGATCGGTTAGAATCCGGCG

Downstream 100 bases:

>100_bases
GGCGTGCGCATTTTTCGTTGGTCTCAGTTGCAGGAGGGTGCATGTATAGCGTGTGATCGGGGAGCAGCAATATCGGTGGG
GGGGTTTGACGGTCCTCACC

Product: tRNA pseudouridine 55 synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MCRLSMPDAIVPFAKVSGLTSFAALAQVRRLLGVKKVGHTGTLDRFADGLLLLLVGGFTKLAPVMTRLEKSYEARIQFGV
QTDTLDPEGAVVRCSLFPTFARVRAALPHFTGSIDQVPPEYSALKFGGVRASDRVRRGEAVCMKARRVFVFDLQVLGCEA
DLGEFKKTQAGRGAAIADLDLTRVRAVTLYVRCSAGFYVRALARDIAAACGSCAYVSHLRRTRIGPFDLAQAAGVSRLGS
WTWGKERASCGAACFDVGAPPPPSSGGVATDSVSFGCEDLTVREIKQAVVSCDVDFANRIGLTACSVHAQYASRFLHGER
IRACWFQSFGTRRPGERALVFSEGRCLGLIRKAANGFSYDAVFCTE

Sequences:

>Translated_366_residues
MCRLSMPDAIVPFAKVSGLTSFAALAQVRRLLGVKKVGHTGTLDRFADGLLLLLVGGFTKLAPVMTRLEKSYEARIQFGV
QTDTLDPEGAVVRCSLFPTFARVRAALPHFTGSIDQVPPEYSALKFGGVRASDRVRRGEAVCMKARRVFVFDLQVLGCEA
DLGEFKKTQAGRGAAIADLDLTRVRAVTLYVRCSAGFYVRALARDIAAACGSCAYVSHLRRTRIGPFDLAQAAGVSRLGS
WTWGKERASCGAACFDVGAPPPPSSGGVATDSVSFGCEDLTVREIKQAVVSCDVDFANRIGLTACSVHAQYASRFLHGER
IRACWFQSFGTRRPGERALVFSEGRCLGLIRKAANGFSYDAVFCTE
>Mature_366_residues
MCRLSMPDAIVPFAKVSGLTSFAALAQVRRLLGVKKVGHTGTLDRFADGLLLLLVGGFTKLAPVMTRLEKSYEARIQFGV
QTDTLDPEGAVVRCSLFPTFARVRAALPHFTGSIDQVPPEYSALKFGGVRASDRVRRGEAVCMKARRVFVFDLQVLGCEA
DLGEFKKTQAGRGAAIADLDLTRVRAVTLYVRCSAGFYVRALARDIAAACGSCAYVSHLRRTRIGPFDLAQAAGVSRLGS
WTWGKERASCGAACFDVGAPPPPSSGGVATDSVSFGCEDLTVREIKQAVVSCDVDFANRIGLTACSVHAQYASRFLHGER
IRACWFQSFGTRRPGERALVFSEGRCLGLIRKAANGFSYDAVFCTE

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=201, Percent_Identity=36.81592039801, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI2367200, Length=248, Percent_Identity=27.8225806451613, Blast_Score=103, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6324037, Length=139, Percent_Identity=34.5323741007194, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_TREPA (O83859)

Other databases:

- EMBL:   AE000520
- PIR:   F71269
- RefSeq:   NP_219324.1
- ProteinModelPortal:   O83859
- SMR:   O83859
- IntAct:   O83859
- GeneID:   2611713
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0889
- NMPDR:   fig|243276.1.peg.883
- TIGR:   TP_0889
- HOGENOM:   HBG732148
- OMA:   NIVIHAL
- ProtClustDB:   CLSK2516682
- BioCyc:   TPAL243276:TP_0889-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 39471; Mature: 39471

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: ACT_SITE 44-44

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCRLSMPDAIVPFAKVSGLTSFAALAQVRRLLGVKKVGHTGTLDRFADGLLLLLVGGFTK
CCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCHHH
LAPVMTRLEKSYEARIQFGVQTDTLDPEGAVVRCSLFPTFARVRAALPHFTGSIDQVPPE
HHHHHHHHHHHHHHEEEECCEECCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCC
YSALKFGGVRASDRVRRGEAVCMKARRVFVFDLQVLGCEADLGEFKKTQAGRGAAIADLD
CHHEEECCCCCHHHHHHHHHHHHHHCEEEEEEEEEECCCCCHHHHHHHCCCCCCEEECCH
LTRVRAVTLYVRCSAGFYVRALARDIAAACGSCAYVSHLRRTRIGPFDLAQAAGVSRLGS
HHHEEEEEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHCHHHHCC
WTWGKERASCGAACFDVGAPPPPSSGGVATDSVSFGCEDLTVREIKQAVVSCDVDFANRI
CCCCCHHHCCCCCEECCCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHHC
GLTACSVHAQYASRFLHGERIRACWFQSFGTRRPGERALVFSEGRCLGLIRKAANGFSYD
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHCCCCCCC
AVFCTE
EEEECC
>Mature Secondary Structure
MCRLSMPDAIVPFAKVSGLTSFAALAQVRRLLGVKKVGHTGTLDRFADGLLLLLVGGFTK
CCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCHHH
LAPVMTRLEKSYEARIQFGVQTDTLDPEGAVVRCSLFPTFARVRAALPHFTGSIDQVPPE
HHHHHHHHHHHHHHEEEECCEECCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCC
YSALKFGGVRASDRVRRGEAVCMKARRVFVFDLQVLGCEADLGEFKKTQAGRGAAIADLD
CHHEEECCCCCHHHHHHHHHHHHHHCEEEEEEEEEECCCCCHHHHHHHCCCCCCEEECCH
LTRVRAVTLYVRCSAGFYVRALARDIAAACGSCAYVSHLRRTRIGPFDLAQAAGVSRLGS
HHHEEEEEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHCHHHHCC
WTWGKERASCGAACFDVGAPPPPSSGGVATDSVSFGCEDLTVREIKQAVVSCDVDFANRI
CCCCCHHHCCCCCEECCCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHHC
GLTACSVHAQYASRFLHGERIRACWFQSFGTRRPGERALVFSEGRCLGLIRKAANGFSYD
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHCCCCCCC
AVFCTE
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876