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The map label for this gene is metK

Identifier: 189026019

GI number: 189026019

Start: 862135

End: 863325

Strand: Reverse

Name: metK

Synonym: TPASS_0794

Alternate gene names: 189026019

Gene position: 863325-862135 (Counterclockwise)

Preceding gene: 189026021

Following gene: 189026016

Centisome position: 75.77

GC content: 53.23

Gene sequence:

>1191_bases
ATGGAGACGTTTTTTACCTCAGAGTCTGTGAGTGAGGGTCATCCTGATAAGCTGTGCGACCAGATTTCTGACGCTGTTCT
TGATGCCTGTCTTTCGCAAGATCCTCACAGTTGTGTTGCGTGCGAAACTTTTGCCTCCACGTCCCTTATCCTGATTGGAG
GTGAAATTAGCACGCGGGCGCATATTAATCTTACCCAAATTGCGCGTGATGTTGCCGCTGACATTGGATATGTAAGCGCT
GATGTCGGTCTTGATGCAGCGTCCATGGCTGTTCTTGATATGACTCATCATCAGTCGCCTGATATTGCGCAGGGGGTGCA
CGGTGCAGGACTGAAGGAGTTTGCAGGATCGCAGGGGGCAGGGGATCAGGGGATTATGTTTGGTTTTGCGTGCCGCGAGA
CGCCGGAGTTTATGCCCGCCCCCCTCATGTGCGCGCACGCGGTTGTGCGCTATGCTGCCACGCTTCGTCATGAACGCCGT
GTGCCGTGGCTGCGTCCTGATGCAAAAAGTCAGGTTACCGTACAATACGAGGGACATCGACCGGTACGTATCAGTGCGGT
TGTGTTTTCTCAGCAGCATGATCCGTCACCTTCATACGAAACCATTAGAGAAACGCTCATAGAGGAGATAGTGCGTCCGG
CGCTTGCACCTACAGGTCTGTTAGATGAAAACACGCGTTTTTTTATCAATCCAACCGGTCGTTTTGTCATTGGCGGTCCC
TTTGGGGACACTGGTTTGACCGGGAGAAAGATCATCGTAGACACGTATGGGGGAATGGGCCGCCATGGAGGAGGCTCCTT
TTCAGGTAAGGATGCATCTAAGGTAGATCGTTCTGCAGCGTATATGGCGCGTTATATTGCAAAAAATATTGTGGCAGCCG
ACCTTGCTGAGCGCTGTGAGGTGCAGCTTGCATACGCAATCGGCGTACCATATCCGGTTTCGCTGCGGATAGAAACATTT
GGAACGGCGCGCGCATCTGAGTCACACATCACACACGCGGTGAAAGAGATTTTTGATTTAACCCCAGCGGGTATCGTGCG
CACGTTGGACCTGTGTGCGCCTCGGTACCGCTCGACTGCAGTGTATGGTCACTTTGGGCGCGAACAGTTTCCTTGGGAAC
GCACAGACTGCGTGTGCGACTTACAGCGTGCGGTGCGCCCGTTCGCGCTCTCTGGCCAGATAAAAGAGTAG

Upstream 100 bases:

>100_bases
GTGTAGGCAAAGATGAGCGCAGCGCTGACAAGAGGTGTTGAGTGCACCCTTTACTCCTAGGTTCAGTGAGCTGCGTAATT
TTGAATCGAGGAGTACAGTG

Downstream 100 bases:

>100_bases
CTTCGTTTCTTTTTTGTCTGCGCGGGGCCTGTATCGTTACAGCCCTTCACTTTCTGCCCATGTTACGATGATTGGCTCTA
GGGAATGTATGGAAAACCCA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
METFFTSESVSEGHPDKLCDQISDAVLDACLSQDPHSCVACETFASTSLILIGGEISTRAHINLTQIARDVAADIGYVSA
DVGLDAASMAVLDMTHHQSPDIAQGVHGAGLKEFAGSQGAGDQGIMFGFACRETPEFMPAPLMCAHAVVRYAATLRHERR
VPWLRPDAKSQVTVQYEGHRPVRISAVVFSQQHDPSPSYETIRETLIEEIVRPALAPTGLLDENTRFFINPTGRFVIGGP
FGDTGLTGRKIIVDTYGGMGRHGGGSFSGKDASKVDRSAAYMARYIAKNIVAADLAERCEVQLAYAIGVPYPVSLRIETF
GTARASESHITHAVKEIFDLTPAGIVRTLDLCAPRYRSTAVYGHFGREQFPWERTDCVCDLQRAVRPFALSGQIKE

Sequences:

>Translated_396_residues
METFFTSESVSEGHPDKLCDQISDAVLDACLSQDPHSCVACETFASTSLILIGGEISTRAHINLTQIARDVAADIGYVSA
DVGLDAASMAVLDMTHHQSPDIAQGVHGAGLKEFAGSQGAGDQGIMFGFACRETPEFMPAPLMCAHAVVRYAATLRHERR
VPWLRPDAKSQVTVQYEGHRPVRISAVVFSQQHDPSPSYETIRETLIEEIVRPALAPTGLLDENTRFFINPTGRFVIGGP
FGDTGLTGRKIIVDTYGGMGRHGGGSFSGKDASKVDRSAAYMARYIAKNIVAADLAERCEVQLAYAIGVPYPVSLRIETF
GTARASESHITHAVKEIFDLTPAGIVRTLDLCAPRYRSTAVYGHFGREQFPWERTDCVCDLQRAVRPFALSGQIKE
>Mature_396_residues
METFFTSESVSEGHPDKLCDQISDAVLDACLSQDPHSCVACETFASTSLILIGGEISTRAHINLTQIARDVAADIGYVSA
DVGLDAASMAVLDMTHHQSPDIAQGVHGAGLKEFAGSQGAGDQGIMFGFACRETPEFMPAPLMCAHAVVRYAATLRHERR
VPWLRPDAKSQVTVQYEGHRPVRISAVVFSQQHDPSPSYETIRETLIEEIVRPALAPTGLLDENTRFFINPTGRFVIGGP
FGDTGLTGRKIIVDTYGGMGRHGGGSFSGKDASKVDRSAAYMARYIAKNIVAADLAERCEVQLAYAIGVPYPVSLRIETF
GTARASESHITHAVKEIFDLTPAGIVRTLDLCAPRYRSTAVYGHFGREQFPWERTDCVCDLQRAVRPFALSGQIKE

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=374, Percent_Identity=53.475935828877, Blast_Score=377, Evalue=1e-105,
Organism=Homo sapiens, GI4557737, Length=374, Percent_Identity=52.9411764705882, Blast_Score=367, Evalue=1e-102,
Organism=Escherichia coli, GI1789311, Length=373, Percent_Identity=59.7855227882037, Blast_Score=457, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17551082, Length=375, Percent_Identity=50.1333333333333, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17538498, Length=375, Percent_Identity=50.9333333333333, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17538494, Length=375, Percent_Identity=50.9333333333333, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25145633, Length=375, Percent_Identity=50.1333333333333, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32565905, Length=375, Percent_Identity=47.2, Blast_Score=316, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI32565911, Length=330, Percent_Identity=47.8787878787879, Blast_Score=295, Evalue=4e-80,
Organism=Caenorhabditis elegans, GI25145635, Length=330, Percent_Identity=46.3636363636364, Blast_Score=287, Evalue=7e-78,
Organism=Caenorhabditis elegans, GI32565903, Length=303, Percent_Identity=48.5148514851485, Blast_Score=271, Evalue=5e-73,
Organism=Caenorhabditis elegans, GI32565909, Length=108, Percent_Identity=47.2222222222222, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI32565907, Length=45, Percent_Identity=75.5555555555556, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320710, Length=375, Percent_Identity=53.0666666666667, Blast_Score=383, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6323209, Length=375, Percent_Identity=52.5333333333333, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580527, Length=374, Percent_Identity=49.1978609625668, Blast_Score=339, Evalue=2e-93,
Organism=Drosophila melanogaster, GI24580525, Length=374, Percent_Identity=49.1978609625668, Blast_Score=339, Evalue=2e-93,
Organism=Drosophila melanogaster, GI45552159, Length=374, Percent_Identity=49.7326203208556, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24580529, Length=374, Percent_Identity=49.7326203208556, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24580537, Length=374, Percent_Identity=49.7326203208556, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24580533, Length=374, Percent_Identity=49.7326203208556, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24580535, Length=374, Percent_Identity=49.7326203208556, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24580531, Length=374, Percent_Identity=49.7326203208556, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24580541, Length=362, Percent_Identity=48.3425414364641, Blast_Score=318, Evalue=4e-87,
Organism=Drosophila melanogaster, GI24580539, Length=120, Percent_Identity=49.1666666666667, Blast_Score=118, Evalue=6e-27,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_TREPA (O83772)

Other databases:

- EMBL:   AE000520
- PIR:   A71281
- RefSeq:   NP_219231.1
- ProteinModelPortal:   O83772
- SMR:   O83772
- IntAct:   O83772
- GeneID:   2610789
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0794
- NMPDR:   fig|243276.1.peg.790
- TIGR:   TP_0794
- HOGENOM:   HBG443662
- OMA:   QDEHARV
- ProtClustDB:   PRK05250
- BioCyc:   TPAL243276:TP_0794-MONOMER
- BRENDA:   2.5.1.6
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42991; Mature: 42991

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METFFTSESVSEGHPDKLCDQISDAVLDACLSQDPHSCVACETFASTSLILIGGEISTRA
CCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHCCCEEEEECCCCCCEE
HINLTQIARDVAADIGYVSADVGLDAASMAVLDMTHHQSPDIAQGVHGAGLKEFAGSQGA
ECCHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHCCCCCCHHHCCCCCCHHHHCCCCCC
GDQGIMFGFACRETPEFMPAPLMCAHAVVRYAATLRHERRVPWLRPDAKSQVTVQYEGHR
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCC
PVRISAVVFSQQHDPSPSYETIRETLIEEIVRPALAPTGLLDENTRFFINPTGRFVIGGP
CEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEEECC
FGDTGLTGRKIIVDTYGGMGRHGGGSFSGKDASKVDRSAAYMARYIAKNIVAADLAERCE
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VQLAYAIGVPYPVSLRIETFGTARASESHITHAVKEIFDLTPAGIVRTLDLCAPRYRSTA
EEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEE
VYGHFGREQFPWERTDCVCDLQRAVRPFALSGQIKE
EEECCCCCCCCCCHHHHHHHHHHHHCCEECCCCCCC
>Mature Secondary Structure
METFFTSESVSEGHPDKLCDQISDAVLDACLSQDPHSCVACETFASTSLILIGGEISTRA
CCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHCCCEEEEECCCCCCEE
HINLTQIARDVAADIGYVSADVGLDAASMAVLDMTHHQSPDIAQGVHGAGLKEFAGSQGA
ECCHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHCCCCCCHHHCCCCCCHHHHCCCCCC
GDQGIMFGFACRETPEFMPAPLMCAHAVVRYAATLRHERRVPWLRPDAKSQVTVQYEGHR
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCC
PVRISAVVFSQQHDPSPSYETIRETLIEEIVRPALAPTGLLDENTRFFINPTGRFVIGGP
CEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEEECC
FGDTGLTGRKIIVDTYGGMGRHGGGSFSGKDASKVDRSAAYMARYIAKNIVAADLAERCE
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VQLAYAIGVPYPVSLRIETFGTARASESHITHAVKEIFDLTPAGIVRTLDLCAPRYRSTA
EEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEE
VYGHFGREQFPWERTDCVCDLQRAVRPFALSGQIKE
EEECCCCCCCCCCHHHHHHHHHHHHCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876