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The map label for this gene is pheS

Identifier: 188586432

GI number: 188586432

Start: 1891147

End: 1892172

Strand: Reverse

Name: pheS

Synonym: Nther_1817

Alternate gene names: 188586432

Gene position: 1892172-1891147 (Counterclockwise)

Preceding gene: 188586433

Following gene: 188586431

Centisome position: 59.77

GC content: 37.23

Gene sequence:

>1026_bases
ATGCAAGAGGAATTAAAAGAATTAAAAGAAAAAGCCCTGGTAGACATAAGTGAAGCCTCTAGTTTGGATAAATTAAAGGA
TGTACAGGTAAAGTATTTAGGTAAAAAAGGCGAATTAACAAAAATATTAAGAGGAATGGGCAATTTATCGCCTGAAGAAC
GACCTGTGGTAGGAAAATTAGCTAATGAAATTAGGGATGATTTAGAACAAGCTTTTGAAGAGCGAGAAAACAATTTGAAA
GAAGCTGAAATGCAAAAACGCTGGGAAGAAGAAAAAATCGACGTAACATTACCTGGAAGAGTCATTAGTCGAGGTGCGAA
ACACCCTTTAAAAAAAGTCCTAGATGAAGTTCATGATATTTTTCTAGGCATGGGATATAACATAGAGGAAGGCCCTGAAA
TTGAAACCGATTATTACAACTTTGAAGCGCTAAACATTCCTAAAGGGCATCCAGCCAGGGAAATGCAAGATTCTTTGTAT
ATTACCGAGGAAATTTTACTCCGGACACATACTTCACCAGTACAAATCCGAACAATGGAACAAGTGGCACCAGAAATCCC
TGTAAGAATTATTTGTACAGGCAAAGTTTATCGTAAAGATGATGATGCTACTCACTCTCCTATGTTTCACCAAATCGAAG
GACTTGTAGTTGGTGAACGGATTACATTGGGGGACCTAAAAGGTACTTTACTTAACTTTGCAAAACAAATGTTTGGTCCA
GAAGTTGACATCAGATTACGTCCTAGTTATTTCCCTTTTACAGAGCCAAGTGCCGAAGTTGATATTTCTTGTGTGATATG
TGAAGGAAGTGGATGTCGTGTTTGTAAAGGAACAGGGTGGCTCGAAATATTAGGTTCTGGAATGGTTCATCCTAAAGTTT
TTGAAATGTCAGGCTATGATCCGGATAAAGTTACTGGTTTCGCTTTTGGAATGGGAGTAGAACGGATAGCCATGCTTAAA
TACGGTATCCAAGATTTACGTATTTTCTTTGAAAATGACAAGCGAATGGTTAATCAATTTAAATAA

Upstream 100 bases:

>100_bases
TTCGTCCCATTGTGACGAAAGCTTTTTTGTTTGGATAAATTTTCTATAGTTCCAAGAGATAAATTATTTTAAATGAGAGT
ATGACAAGGAGGTTGTTGTA

Downstream 100 bases:

>100_bases
ACTGTTATCGCGAAATAGACGGATTACTTAAATCAAATTAAATTCTAATGTAAGGGGTAAATTAAAGTAAAAAATTGGAG
GGTGTTGTAATATGCAAGTT

Product: phenylalanyl-tRNA synthetase, alpha subunit

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MQEELKELKEKALVDISEASSLDKLKDVQVKYLGKKGELTKILRGMGNLSPEERPVVGKLANEIRDDLEQAFEERENNLK
EAEMQKRWEEEKIDVTLPGRVISRGAKHPLKKVLDEVHDIFLGMGYNIEEGPEIETDYYNFEALNIPKGHPAREMQDSLY
ITEEILLRTHTSPVQIRTMEQVAPEIPVRIICTGKVYRKDDDATHSPMFHQIEGLVVGERITLGDLKGTLLNFAKQMFGP
EVDIRLRPSYFPFTEPSAEVDISCVICEGSGCRVCKGTGWLEILGSGMVHPKVFEMSGYDPDKVTGFAFGMGVERIAMLK
YGIQDLRIFFENDKRMVNQFK

Sequences:

>Translated_341_residues
MQEELKELKEKALVDISEASSLDKLKDVQVKYLGKKGELTKILRGMGNLSPEERPVVGKLANEIRDDLEQAFEERENNLK
EAEMQKRWEEEKIDVTLPGRVISRGAKHPLKKVLDEVHDIFLGMGYNIEEGPEIETDYYNFEALNIPKGHPAREMQDSLY
ITEEILLRTHTSPVQIRTMEQVAPEIPVRIICTGKVYRKDDDATHSPMFHQIEGLVVGERITLGDLKGTLLNFAKQMFGP
EVDIRLRPSYFPFTEPSAEVDISCVICEGSGCRVCKGTGWLEILGSGMVHPKVFEMSGYDPDKVTGFAFGMGVERIAMLK
YGIQDLRIFFENDKRMVNQFK
>Mature_341_residues
MQEELKELKEKALVDISEASSLDKLKDVQVKYLGKKGELTKILRGMGNLSPEERPVVGKLANEIRDDLEQAFEERENNLK
EAEMQKRWEEEKIDVTLPGRVISRGAKHPLKKVLDEVHDIFLGMGYNIEEGPEIETDYYNFEALNIPKGHPAREMQDSLY
ITEEILLRTHTSPVQIRTMEQVAPEIPVRIICTGKVYRKDDDATHSPMFHQIEGLVVGERITLGDLKGTLLNFAKQMFGP
EVDIRLRPSYFPFTEPSAEVDISCVICEGSGCRVCKGTGWLEILGSGMVHPKVFEMSGYDPDKVTGFAFGMGVERIAMLK
YGIQDLRIFFENDKRMVNQFK

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=269, Percent_Identity=31.5985130111524, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI5729820, Length=291, Percent_Identity=30.2405498281787, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1788007, Length=338, Percent_Identity=49.4082840236686, Blast_Score=339, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI17508957, Length=275, Percent_Identity=30.1818181818182, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32563657, Length=275, Percent_Identity=30.1818181818182, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI32566635, Length=296, Percent_Identity=32.4324324324324, Blast_Score=127, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6321087, Length=372, Percent_Identity=27.9569892473118, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6325304, Length=279, Percent_Identity=26.5232974910394, Blast_Score=96, Evalue=7e-21,
Organism=Drosophila melanogaster, GI18858079, Length=270, Percent_Identity=30.3703703703704, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI17137424, Length=292, Percent_Identity=28.0821917808219, Blast_Score=118, Evalue=6e-27,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_NATTJ (B2A5N5)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917977.1
- ProteinModelPortal:   B2A5N5
- SMR:   B2A5N5
- GeneID:   6316999
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1817
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 38785; Mature: 38785

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEELKELKEKALVDISEASSLDKLKDVQVKYLGKKGELTKILRGMGNLSPEERPVVGKL
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHCCCCCCCCCHHHHH
ANEIRDDLEQAFEERENNLKEAEMQKRWEEEKIDVTLPGRVISRGAKHPLKKVLDEVHDI
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCEEEEECHHHHHCCCCCHHHHHHHHHHHH
FLGMGYNIEEGPEIETDYYNFEALNIPKGHPAREMQDSLYITEEILLRTHTSPVQIRTME
HHCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHH
QVAPEIPVRIICTGKVYRKDDDATHSPMFHQIEGLVVGERITLGDLKGTLLNFAKQMFGP
HCCCCCCEEEEECCEEEECCCCCCCCCHHHHHCCEEECCEEEEHHHHHHHHHHHHHHCCC
EVDIRLRPSYFPFTEPSAEVDISCVICEGSGCRVCKGTGWLEILGSGMVHPKVFEMSGYD
CEEEEECCCCCCCCCCCCCEEEEEEEECCCCCEEECCCCHHHHHCCCCCCCEEEECCCCC
PDKVTGFAFGMGVERIAMLKYGIQDLRIFFENDKRMVNQFK
CCHHHHHHHHHHHHHHHHHHHCHHHHHEEHHCCHHHHHHCC
>Mature Secondary Structure
MQEELKELKEKALVDISEASSLDKLKDVQVKYLGKKGELTKILRGMGNLSPEERPVVGKL
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHCCCCCCCCCHHHHH
ANEIRDDLEQAFEERENNLKEAEMQKRWEEEKIDVTLPGRVISRGAKHPLKKVLDEVHDI
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCEEEEECHHHHHCCCCCHHHHHHHHHHHH
FLGMGYNIEEGPEIETDYYNFEALNIPKGHPAREMQDSLYITEEILLRTHTSPVQIRTME
HHCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHH
QVAPEIPVRIICTGKVYRKDDDATHSPMFHQIEGLVVGERITLGDLKGTLLNFAKQMFGP
HCCCCCCEEEEECCEEEECCCCCCCCCHHHHHCCEEECCEEEEHHHHHHHHHHHHHHCCC
EVDIRLRPSYFPFTEPSAEVDISCVICEGSGCRVCKGTGWLEILGSGMVHPKVFEMSGYD
CEEEEECCCCCCCCCCCCCEEEEEEEECCCCCEEECCCCHHHHHCCCCCCCEEEECCCCC
PDKVTGFAFGMGVERIAMLKYGIQDLRIFFENDKRMVNQFK
CCHHHHHHHHHHHHHHHHHHHCHHHHHEEHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA