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The map label for this gene is sppA [H]

Identifier: 188586361

GI number: 188586361

Start: 1810153

End: 1811853

Strand: Reverse

Name: sppA [H]

Synonym: Nther_1744

Alternate gene names: 188586361

Gene position: 1811853-1810153 (Counterclockwise)

Preceding gene: 188586362

Following gene: 188586360

Centisome position: 57.24

GC content: 32.98

Gene sequence:

>1701_bases
ATGTTATTATCCCATGTGTATTCAGGGTTTAGATGGATATATTATGGTTGTTCTTTTATTAAAAGAACTTTAACCAGGCC
CGCGGATTACATAATAATCGAGTTAGAAGGTAATTATAATTTTTTTCCTGAACCCCAAAATTTTTTAAAAAAAAGATTTG
CTAAAACCCCTCCGGCAATATTAAATTTAAAAAAACAATTGAAAACTATCAGTAACGATCCTAGATTAACAGGTGTGGTT
TTGAAACAGACCAATTTTAATATACCATACACTTATTTAGATGAAGTTATAGACGCTTTAAAAATCCTGAAAAATAACAA
TAAAAAGTTATACTCCTGGGCAAAAAATTATAGTACCAGTGGTTATTTTTTAGCTAGTTATTGTGACGAAATATTTTTGC
AACCTGGTGGAAAAATTGATGAGTTGGGTTTTCTCAATGAGAGTTTTTTTCTTAAGGATGCTCTTAATCAATTGGGCATT
GAATTTGATATTTTTAAAATCAGTCCTTATAAAACAGCTTCAGACTTTCTGATTCGGTCTAACTCTTCTAAAGAAGCCCG
AGAGATGAATCAGTGGATTATGAACTCTATATATGACAATTTCATTAATAATATATCTTGGGGCAGAAAAAAGACTAAAC
AGGAGATGGAAGAATTAGTCAATAATGCTCCCTATACAGATAACGAAAGCCTTGAATTAGGATTGATAGATAAAATCTTG
GGAGAAGAAGATTTAGTTAAATTTTTAAAACCGCTCGATAAAAAAGAGTCGAAAAAAATACAACACTCAATAAAAATTGA
GAACTTTAAGAGTGCTCATAAAAAACTATATCCACCTAGACCTAAAAAGTCAAAAGGTAGTGTTGGACTTGTAAAAATAG
AGGGTATGATAATAGATGGTAGGAGCAAAGCCCCGCAAAATTATCCTCCCATTCCAATTCCCTTAATTGCTGGTGAACGT
GCTGGCGATCTCACCATTACCGAACAATTAAGGAAACTGGGTGCAAATCCCAAAATTAAGGGGATAATTGTATTTATTGA
TTCAGGAGGCGGTTCGGCTTTGGCTTCTGAGTCTATTGCTTACACTATAAGGTCTATTACTAGACAAAAACCAGTAATTG
CTTTATTAGGTGAAAAAGCAGCATCCGGGGGATATTATGTGGCATCACAAGCTGATTATATAGTGTCCTACCCCACTACA
CTTACTGGATCCATAGGTGTAGTTGCCGGTAAGGCAGTTAATACAAATTTACTAAATAAATTATTGATTAACCGAGAAAT
CAATGTAAGAGGAAACAGCAGAGAAATGTTTTACAGTCATAGAGTTTTCAATGATAGAGAACGGGAAAAAATGCAAGAAG
GATTAATGCGAACATACAATCTATTTTTAGAAAGAGTTTGTGAGGGCAGGAAAATGACTAAAGCCCAGTTAGATCCTATA
GCAAAAGGTAAAGTCTGGACTGGGGAACAAGCTTTAGCCAAAGGATTAGTTGATGAGTTAGGTGGATTTGAAGAAACAAA
AGTTAAAATGAAAGAGTTAACAGGTTTATCTAAATTTTCGATTTATGAACCAAAAATCACCGAAAGGTATCAAATATCAA
AATTTGATGAACAACAAAATATCGTAAATTATTCCATAGAAGGACTAAAATTATTTGATGGTAAAAAACCGTTATGTATA
GCTGAGGAATTGGTATATTAA

Upstream 100 bases:

>100_bases
TACTGTCCCAACCACCAACTATCAAGGATATTAAAGCAGCTAGAAAATCAGCCCATGATAGAGGACTTAATCTTTTGGAT
GATTGATAAGAGGTGATATA

Downstream 100 bases:

>100_bases
AGTGTGTAGAAGTTAGAAGTTGATAACTAAAGAATATCAATTCGCTAAGCTTATGAATTTATTAATGTAAGGATTAATGC
AAAAATTATAAGGGAGGGGA

Product: Acid phosphatase

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV; Signal peptide peptidase [H]

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MLLSHVYSGFRWIYYGCSFIKRTLTRPADYIIIELEGNYNFFPEPQNFLKKRFAKTPPAILNLKKQLKTISNDPRLTGVV
LKQTNFNIPYTYLDEVIDALKILKNNNKKLYSWAKNYSTSGYFLASYCDEIFLQPGGKIDELGFLNESFFLKDALNQLGI
EFDIFKISPYKTASDFLIRSNSSKEAREMNQWIMNSIYDNFINNISWGRKKTKQEMEELVNNAPYTDNESLELGLIDKIL
GEEDLVKFLKPLDKKESKKIQHSIKIENFKSAHKKLYPPRPKKSKGSVGLVKIEGMIIDGRSKAPQNYPPIPIPLIAGER
AGDLTITEQLRKLGANPKIKGIIVFIDSGGGSALASESIAYTIRSITRQKPVIALLGEKAASGGYYVASQADYIVSYPTT
LTGSIGVVAGKAVNTNLLNKLLINREINVRGNSREMFYSHRVFNDREREKMQEGLMRTYNLFLERVCEGRKMTKAQLDPI
AKGKVWTGEQALAKGLVDELGGFEETKVKMKELTGLSKFSIYEPKITERYQISKFDEQQNIVNYSIEGLKLFDGKKPLCI
AEELVY

Sequences:

>Translated_566_residues
MLLSHVYSGFRWIYYGCSFIKRTLTRPADYIIIELEGNYNFFPEPQNFLKKRFAKTPPAILNLKKQLKTISNDPRLTGVV
LKQTNFNIPYTYLDEVIDALKILKNNNKKLYSWAKNYSTSGYFLASYCDEIFLQPGGKIDELGFLNESFFLKDALNQLGI
EFDIFKISPYKTASDFLIRSNSSKEAREMNQWIMNSIYDNFINNISWGRKKTKQEMEELVNNAPYTDNESLELGLIDKIL
GEEDLVKFLKPLDKKESKKIQHSIKIENFKSAHKKLYPPRPKKSKGSVGLVKIEGMIIDGRSKAPQNYPPIPIPLIAGER
AGDLTITEQLRKLGANPKIKGIIVFIDSGGGSALASESIAYTIRSITRQKPVIALLGEKAASGGYYVASQADYIVSYPTT
LTGSIGVVAGKAVNTNLLNKLLINREINVRGNSREMFYSHRVFNDREREKMQEGLMRTYNLFLERVCEGRKMTKAQLDPI
AKGKVWTGEQALAKGLVDELGGFEETKVKMKELTGLSKFSIYEPKITERYQISKFDEQQNIVNYSIEGLKLFDGKKPLCI
AEELVY
>Mature_566_residues
MLLSHVYSGFRWIYYGCSFIKRTLTRPADYIIIELEGNYNFFPEPQNFLKKRFAKTPPAILNLKKQLKTISNDPRLTGVV
LKQTNFNIPYTYLDEVIDALKILKNNNKKLYSWAKNYSTSGYFLASYCDEIFLQPGGKIDELGFLNESFFLKDALNQLGI
EFDIFKISPYKTASDFLIRSNSSKEAREMNQWIMNSIYDNFINNISWGRKKTKQEMEELVNNAPYTDNESLELGLIDKIL
GEEDLVKFLKPLDKKESKKIQHSIKIENFKSAHKKLYPPRPKKSKGSVGLVKIEGMIIDGRSKAPQNYPPIPIPLIAGER
AGDLTITEQLRKLGANPKIKGIIVFIDSGGGSALASESIAYTIRSITRQKPVIALLGEKAASGGYYVASQADYIVSYPTT
LTGSIGVVAGKAVNTNLLNKLLINREINVRGNSREMFYSHRVFNDREREKMQEGLMRTYNLFLERVCEGRKMTKAQLDPI
AKGKVWTGEQALAKGLVDELGGFEETKVKMKELTGLSKFSIYEPKITERYQISKFDEQQNIVNYSIEGLKLFDGKKPLCI
AEELVY

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=473, Percent_Identity=29.1754756871036, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1787527, Length=142, Percent_Identity=34.5070422535211, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 64316; Mature: 64316

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLSHVYSGFRWIYYGCSFIKRTLTRPADYIIIELEGNYNFFPEPQNFLKKRFAKTPPAI
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCHHH
LNLKKQLKTISNDPRLTGVVLKQTNFNIPYTYLDEVIDALKILKNNNKKLYSWAKNYSTS
HHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
GYFLASYCDEIFLQPGGKIDELGFLNESFFLKDALNQLGIEFDIFKISPYKTASDFLIRS
CEEHHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEC
NSSKEAREMNQWIMNSIYDNFINNISWGRKKTKQEMEELVNNAPYTDNESLELGLIDKIL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHH
GEEDLVKFLKPLDKKESKKIQHSIKIENFKSAHKKLYPPRPKKSKGSVGLVKIEGMIIDG
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEC
RSKAPQNYPPIPIPLIAGERAGDLTITEQLRKLGANPKIKGIIVFIDSGGGSALASESIA
CCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHHH
YTIRSITRQKPVIALLGEKAASGGYYVASQADYIVSYPTTLTGSIGVVAGKAVNTNLLNK
HHHHHHHCCCCEEEEECCCCCCCCEEEECCCCEEEECCCEECCCCCEEECCCCCHHHHHH
LLINREINVRGNSREMFYSHRVFNDREREKMQEGLMRTYNLFLERVCEGRKMTKAQLDPI
HHHCCEEECCCCCHHHEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCC
AKGKVWTGEQALAKGLVDELGGFEETKVKMKELTGLSKFSIYEPKITERYQISKFDEQQN
CCCCEECCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEECCCCCCCEECCHHCCCCC
IVNYSIEGLKLFDGKKPLCIAEELVY
CEEEEECCEEEECCCCCCCHHHHHCC
>Mature Secondary Structure
MLLSHVYSGFRWIYYGCSFIKRTLTRPADYIIIELEGNYNFFPEPQNFLKKRFAKTPPAI
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCHHH
LNLKKQLKTISNDPRLTGVVLKQTNFNIPYTYLDEVIDALKILKNNNKKLYSWAKNYSTS
HHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
GYFLASYCDEIFLQPGGKIDELGFLNESFFLKDALNQLGIEFDIFKISPYKTASDFLIRS
CEEHHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEC
NSSKEAREMNQWIMNSIYDNFINNISWGRKKTKQEMEELVNNAPYTDNESLELGLIDKIL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHH
GEEDLVKFLKPLDKKESKKIQHSIKIENFKSAHKKLYPPRPKKSKGSVGLVKIEGMIIDG
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEC
RSKAPQNYPPIPIPLIAGERAGDLTITEQLRKLGANPKIKGIIVFIDSGGGSALASESIA
CCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHHH
YTIRSITRQKPVIALLGEKAASGGYYVASQADYIVSYPTTLTGSIGVVAGKAVNTNLLNK
HHHHHHHCCCCEEEEECCCCCCCCEEEECCCCEEEECCCEECCCCCEEECCCCCHHHHHH
LLINREINVRGNSREMFYSHRVFNDREREKMQEGLMRTYNLFLERVCEGRKMTKAQLDPI
HHHCCEEECCCCCHHHEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCC
AKGKVWTGEQALAKGLVDELGGFEETKVKMKELTGLSKFSIYEPKITERYQISKFDEQQN
CCCCEECCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEECCCCCCCEECCHHCCCCC
IVNYSIEGLKLFDGKKPLCIAEELVY
CEEEEECCEEEECCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]