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The map label for this gene is sigF [H]

Identifier: 188586278

GI number: 188586278

Start: 1738228

End: 1738989

Strand: Reverse

Name: sigF [H]

Synonym: Nther_1661

Alternate gene names: 188586278

Gene position: 1738989-1738228 (Counterclockwise)

Preceding gene: 188586279

Following gene: 188586277

Centisome position: 54.93

GC content: 31.5

Gene sequence:

>762_bases
ATGAGGGCAAATACTCATAAATATCATAAAGCAACTGATAATACTATTGAATTAATCAAGAAATGGCAACAAAAAGGAGA
TAGAGAAGCGAGAAAGATAATTATAGAGGAAAACATTAATTTGGTAAAATCAGTTGTGAAAAGATTTGTAGGTTCTGGCA
TCGAAGAAGAAGATTTAACTCAACTTGGTTTAATGGGATTGATTAAGGCTATTGATAAATTTGATTATCGTAAAGAAGTG
AGATTTTCGACCTATGCAGTTCCTTTGATTATTGGTGAAATTAAAACTTTTTTGCGTGATGACCATCCAATAAAAGTAAG
TAGAAATCTTAAAGAATTATTTAATCGCTCTAAAGAAGTCAAAAGATGGTTAACTCTTAAATTGCGTAGAGAGCCAACTA
TTAGTGAAGTAGCAGACGAGTTAGAAGTATCAAAAGAAGAACTGGCAACTGCTGAAGAAGCTAATCAAGAATTGATTTCT
TTAGATAAACCAGTATCTGATGAGCATAATCACGAAAGCGAGAAAGCTCTGATTGATACTATTCCCGATAACTCTTTTGA
TGCTAAAATTCATAATGAATTAACAATTCAAGAATTACTGAAAAAACTGGATCAAAAAAAGAGAAGATTAATTGTTTTGA
GATATTTTTTGGAAAAAAGTCAACAAGAGACTGGAGTAATACTAGGGATGTCACAAGTTCAGGTTTCGAGAATGGAACGC
CGTATTCTCAATAACTTACAGCGTGATTTAATTGATAATTAA

Upstream 100 bases:

>100_bases
AAAATTTTATGGATTCTGTGGAGATCACTTCTAATTTGAATCAGGGAACAACTGTGAGAATGAAGAAAAAATTTAGTTCT
TCAATTAAGGAGTAATTGTT

Downstream 100 bases:

>100_bases
TTATTATAGTATAACTAGAACTTTATGTGAAAAACTATGAATAAAGATGGCAAAGGAGGTAACAATTTGGATACCGTAAA
AGTAGTAGAATTAGTTGGCG

Product: RNA polymerase, sigma 28 subunit, FliA/WhiG family

Products: NA

Alternate protein names: Sporulation sigma factor; Stage II sporulation protein AC [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MRANTHKYHKATDNTIELIKKWQQKGDREARKIIIEENINLVKSVVKRFVGSGIEEEDLTQLGLMGLIKAIDKFDYRKEV
RFSTYAVPLIIGEIKTFLRDDHPIKVSRNLKELFNRSKEVKRWLTLKLRREPTISEVADELEVSKEELATAEEANQELIS
LDKPVSDEHNHESEKALIDTIPDNSFDAKIHNELTIQELLKKLDQKKRRLIVLRYFLEKSQQETGVILGMSQVQVSRMER
RILNNLQRDLIDN

Sequences:

>Translated_253_residues
MRANTHKYHKATDNTIELIKKWQQKGDREARKIIIEENINLVKSVVKRFVGSGIEEEDLTQLGLMGLIKAIDKFDYRKEV
RFSTYAVPLIIGEIKTFLRDDHPIKVSRNLKELFNRSKEVKRWLTLKLRREPTISEVADELEVSKEELATAEEANQELIS
LDKPVSDEHNHESEKALIDTIPDNSFDAKIHNELTIQELLKKLDQKKRRLIVLRYFLEKSQQETGVILGMSQVQVSRMER
RILNNLQRDLIDN
>Mature_253_residues
MRANTHKYHKATDNTIELIKKWQQKGDREARKIIIEENINLVKSVVKRFVGSGIEEEDLTQLGLMGLIKAIDKFDYRKEV
RFSTYAVPLIIGEIKTFLRDDHPIKVSRNLKELFNRSKEVKRWLTLKLRREPTISEVADELEVSKEELATAEEANQELIS
LDKPVSDEHNHESEKALIDTIPDNSFDAKIHNELTIQELLKKLDQKKRRLIVLRYFLEKSQQETGVILGMSQVQVSRMER
RILNNLQRDLIDN

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=227, Percent_Identity=25.5506607929515, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789098, Length=232, Percent_Identity=27.5862068965517, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI1789871, Length=255, Percent_Identity=25.8823529411765, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014236
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29598; Mature: 29598

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRANTHKYHKATDNTIELIKKWQQKGDREARKIIIEENINLVKSVVKRFVGSGIEEEDLT
CCCCCCCHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHH
QLGLMGLIKAIDKFDYRKEVRFSTYAVPLIIGEIKTFLRDDHPIKVSRNLKELFNRSKEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
KRWLTLKLRREPTISEVADELEVSKEELATAEEANQELISLDKPVSDEHNHESEKALIDT
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHC
IPDNSFDAKIHNELTIQELLKKLDQKKRRLIVLRYFLEKSQQETGVILGMSQVQVSRMER
CCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHH
RILNNLQRDLIDN
HHHHHHHHHHCCC
>Mature Secondary Structure
MRANTHKYHKATDNTIELIKKWQQKGDREARKIIIEENINLVKSVVKRFVGSGIEEEDLT
CCCCCCCHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHH
QLGLMGLIKAIDKFDYRKEVRFSTYAVPLIIGEIKTFLRDDHPIKVSRNLKELFNRSKEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
KRWLTLKLRREPTISEVADELEVSKEELATAEEANQELISLDKPVSDEHNHESEKALIDT
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHC
IPDNSFDAKIHNELTIQELLKKLDQKKRRLIVLRYFLEKSQQETGVILGMSQVQVSRMER
CCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHH
RILNNLQRDLIDN
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2513372 [H]