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The map label for this gene is dacB [H]

Identifier: 188586265

GI number: 188586265

Start: 1726709

End: 1727872

Strand: Reverse

Name: dacB [H]

Synonym: Nther_1648

Alternate gene names: 188586265

Gene position: 1727872-1726709 (Counterclockwise)

Preceding gene: 188586266

Following gene: 188586264

Centisome position: 54.58

GC content: 36.6

Gene sequence:

>1164_bases
ATGCCTGTGAAGCATGAACCAGTACTAGTTAAATTAATAGCTGGTTTAATTATTGGATTACTGGTATTGGCTAATTCCAA
TTTGACTAATGCTCTACCCGAAGAAGATTTATATGAGCTAAAAGAAATAGAAGAACTAGAAGAAGAAATTACAGCCACTA
GCGCATGTTTAGTTGATGTCAAAACTGGTAGAGTTCTATACGAACAAAATAGTCATAAACAATTACCTATGGCGAGTACT
ACAAAGGTAATGACAGCTTTATTATCTTTAGAACGTGGTAATTTAAACGAAGTAGTGACAGCCAGTGAAGCTGCTTCAGA
GGTGGAAGGGTCTTCAATTTACTTACAGGAAGATGAACAGTTAACTCTAGAAGCTATGTTGTATGGATTAATGCTAGAAT
CGGGCAATGATGCGGCACATGCCATTGCGGAACACATTGATGGTGATATAGAAAGTTTCGCACGAAATATGACGGACAGA
GCTGAATCCCTGGGAGCGAAAAATACAAACTTTCAAAATCCTCATGGTTTACCTTCCGACAATCATTATTCCACGGCTTA
TGATATGACTATTATTGCTAAAGAAGCTCTAAAGAAAAGTAAGTTTGCTGAAATTGTAAGTACCAAGGAAAAACTTATTC
CTTGGGAAAAAGGAGATGCTGACTGGAGATACTTAAACAATGATAATAAACTGTTGGATGAGTTGAGTGAAGCCGACGGT
GTTAAAACTGGATGGACAGAAGCTGCTGGAAGATGTCTCATTTTTTCAGGAACAAAAGATGAAAGGCAGGTAGTGGGAAC
AGTTTTAAAGGCTCCAAAAATGTATCAAGATGCAGAAAACTTACTTGAGTACGGAATATATCAATTTAATGAGAAACAGG
TAATTCAAAAAGGACAATTAGTGCGGGAAGTTCCGGTGGAAAAAGGGAAATATTCAACTGTTCCTGTGGTAGCTGGTAGT
GAAATTGTTTTACCTTTAGAATCACAGGAGTATCGAGATTTAAACTATCATATAAAAATTCCCAAAAAATTACAAGCTCC
TTTGGAGAAAGGAGAAGAGATAGGTACGTTGCAAATATTATCTGGCAAATGCACTTTAGCTGAAACTGAACTTTTAACTT
CAAAAGAAGTTGATGAACCCTTTTACAGAAGATTTCTGCCATAA

Upstream 100 bases:

>100_bases
CTAATGACCCTCGAGTTCAATGACTCGAGGGCATATTTTTTTATAATAGTTAATATATTTAAAATCAGAAATATAGCTTA
AAAAATAAGGAGTTGAGTTC

Downstream 100 bases:

>100_bases
ATGGGGTGAGTAGATTTGATAAATATTATATGGCTCACCTTTATAATAGGTGGTTTTATTTTTGCTTTTATTAATGACAA
TGTAGAAGAAGTTACTCAAG

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MPVKHEPVLVKLIAGLIIGLLVLANSNLTNALPEEDLYELKEIEELEEEITATSACLVDVKTGRVLYEQNSHKQLPMAST
TKVMTALLSLERGNLNEVVTASEAASEVEGSSIYLQEDEQLTLEAMLYGLMLESGNDAAHAIAEHIDGDIESFARNMTDR
AESLGAKNTNFQNPHGLPSDNHYSTAYDMTIIAKEALKKSKFAEIVSTKEKLIPWEKGDADWRYLNNDNKLLDELSEADG
VKTGWTEAAGRCLIFSGTKDERQVVGTVLKAPKMYQDAENLLEYGIYQFNEKQVIQKGQLVREVPVEKGKYSTVPVVAGS
EIVLPLESQEYRDLNYHIKIPKKLQAPLEKGEEIGTLQILSGKCTLAETELLTSKEVDEPFYRRFLP

Sequences:

>Translated_387_residues
MPVKHEPVLVKLIAGLIIGLLVLANSNLTNALPEEDLYELKEIEELEEEITATSACLVDVKTGRVLYEQNSHKQLPMAST
TKVMTALLSLERGNLNEVVTASEAASEVEGSSIYLQEDEQLTLEAMLYGLMLESGNDAAHAIAEHIDGDIESFARNMTDR
AESLGAKNTNFQNPHGLPSDNHYSTAYDMTIIAKEALKKSKFAEIVSTKEKLIPWEKGDADWRYLNNDNKLLDELSEADG
VKTGWTEAAGRCLIFSGTKDERQVVGTVLKAPKMYQDAENLLEYGIYQFNEKQVIQKGQLVREVPVEKGKYSTVPVVAGS
EIVLPLESQEYRDLNYHIKIPKKLQAPLEKGEEIGTLQILSGKCTLAETELLTSKEVDEPFYRRFLP
>Mature_386_residues
PVKHEPVLVKLIAGLIIGLLVLANSNLTNALPEEDLYELKEIEELEEEITATSACLVDVKTGRVLYEQNSHKQLPMASTT
KVMTALLSLERGNLNEVVTASEAASEVEGSSIYLQEDEQLTLEAMLYGLMLESGNDAAHAIAEHIDGDIESFARNMTDRA
ESLGAKNTNFQNPHGLPSDNHYSTAYDMTIIAKEALKKSKFAEIVSTKEKLIPWEKGDADWRYLNNDNKLLDELSEADGV
KTGWTEAAGRCLIFSGTKDERQVVGTVLKAPKMYQDAENLLEYGIYQFNEKQVIQKGQLVREVPVEKGKYSTVPVVAGSE
IVLPLESQEYRDLNYHIKIPKKLQAPLEKGEEIGTLQILSGKCTLAETELLTSKEVDEPFYRRFLP

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=347, Percent_Identity=29.6829971181556, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI87082022, Length=351, Percent_Identity=28.2051282051282, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1787062, Length=354, Percent_Identity=30.7909604519774, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI87082054, Length=227, Percent_Identity=28.1938325991189, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 43091; Mature: 42960

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVKHEPVLVKLIAGLIIGLLVLANSNLTNALPEEDLYELKEIEELEEEITATSACLVDV
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEE
KTGRVLYEQNSHKQLPMASTTKVMTALLSLERGNLNEVVTASEAASEVEGSSIYLQEDEQ
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHEEHHHHHHHCCCCEEEEECCCH
LTLEAMLYGLMLESGNDAAHAIAEHIDGDIESFARNMTDRAESLGAKNTNFQNPHGLPSD
HHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
NHYSTAYDMTIIAKEALKKSKFAEIVSTKEKLIPWEKGDADWRYLNNDNKLLDELSEADG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHCC
VKTGWTEAAGRCLIFSGTKDERQVVGTVLKAPKMYQDAENLLEYGIYQFNEKQVIQKGQL
CCCCCHHHCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
VREVPVEKGKYSTVPVVAGSEIVLPLESQEYRDLNYHIKIPKKLQAPLEKGEEIGTLQIL
HHHCCCCCCCCCEEEEEECCEEEEECCCCCCCCCCEEEECCHHHHHHHHCCCCCCEEEEE
SGKCTLAETELLTSKEVDEPFYRRFLP
CCCCEEHHHHHHHHHCCCCHHHHHCCC
>Mature Secondary Structure 
PVKHEPVLVKLIAGLIIGLLVLANSNLTNALPEEDLYELKEIEELEEEITATSACLVDV
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEE
KTGRVLYEQNSHKQLPMASTTKVMTALLSLERGNLNEVVTASEAASEVEGSSIYLQEDEQ
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHEEHHHHHHHCCCCEEEEECCCH
LTLEAMLYGLMLESGNDAAHAIAEHIDGDIESFARNMTDRAESLGAKNTNFQNPHGLPSD
HHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
NHYSTAYDMTIIAKEALKKSKFAEIVSTKEKLIPWEKGDADWRYLNNDNKLLDELSEADG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHCC
VKTGWTEAAGRCLIFSGTKDERQVVGTVLKAPKMYQDAENLLEYGIYQFNEKQVIQKGQL
CCCCCHHHCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
VREVPVEKGKYSTVPVVAGSEIVLPLESQEYRDLNYHIKIPKKLQAPLEKGEEIGTLQIL
HHHCCCCCCCCCEEEEEECCEEEEECCCCCCCCCCEEEECCHHHHHHHHCCCCCCEEEEE
SGKCTLAETELLTSKEVDEPFYRRFLP
CCCCEEHHHHHHHHHCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]