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The map label for this gene is rsmE [H]

Identifier: 188585812

GI number: 188585812

Start: 1258504

End: 1259226

Strand: Direct

Name: rsmE [H]

Synonym: Nther_1186

Alternate gene names: 188585812

Gene position: 1258504-1259226 (Clockwise)

Preceding gene: 188585811

Following gene: 188585813

Centisome position: 39.76

GC content: 37.21

Gene sequence:

>723_bases
TTGACACGTGTGTTACGTTTGAAAATTGGTGATCGAGTAATTTTGCTAGATGGTAAAGGAGGAGCCTGTTTTAGTTTTAT
AAATAGATTAGGAGAAGGCCAAATTGAATTTGAATTAGAAGAACCATTAATTGATAATAAAGAAGCGCCTATACGAATAA
CCTTGGCCCAATCATTGATTAAAAAAGACAACTTTGAATTTGTACTCCAAAAAGGTACCGAATTGGGAGCGACTGAATTT
TATCCCCTGATAAGTCAACGTACAGAAGTGAATTTATTAAATAAATCAGACAAAAAAGTCTCCAAAAAGCTAACTCGCTG
GCGAGAAATAGTTAAAAACGCTGCAGAGCAATGTCAGAGGCAAGTACTACCTCTAATTCATTACCCCGTCAATTTTGAAA
AGTATCTAAAAAATAACAATGAGAATTCGAACAATAATGGTTCGAGATTAAAAATAATGTGTCATGAACAAGAAAACACC
CCCTTAAAAAAGGTCCTGAAAACTATCGAGACTCCACCGTCTTCTGCTATAATATTGATAGGGCCAGAAGGGGGTTTTTC
CCATAATGAAGTGGATTTAGCTCGGGAACAAGGTTTTTCTTCTGTGAGTATGGGTCCTAGAATACTAAGAGCTGAAACTG
CAGGTATAGTTGCCGTCTCAGCAATTCTATACGAATTAGCAGATTTGGGAGGAACTGAGCAATGTCAGACGTACCAAGGG
TGA

Upstream 100 bases:

>100_bases
AATTAGTGGTCAAATACCAAGAATTTTTTTAGAAGAAAATTCATTAACTGAAGTTAGTGCAGGCCAGAAAATAACAGGTT
CCCGAGAAGATTACCACTAT

Downstream 100 bases:

>100_bases
AATTCATTACTTTTGGATGTAAAGTTAATCAGTATGATTCAGAAGCCCTGAAAGAATTATTCCAGGACAGGGGATTTGAG
ATAAGTGATTGGAATCCTCA

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MTRVLRLKIGDRVILLDGKGGACFSFINRLGEGQIEFELEEPLIDNKEAPIRITLAQSLIKKDNFEFVLQKGTELGATEF
YPLISQRTEVNLLNKSDKKVSKKLTRWREIVKNAAEQCQRQVLPLIHYPVNFEKYLKNNNENSNNNGSRLKIMCHEQENT
PLKKVLKTIETPPSSAIILIGPEGGFSHNEVDLAREQGFSSVSMGPRILRAETAGIVAVSAILYELADLGGTEQCQTYQG

Sequences:

>Translated_240_residues
MTRVLRLKIGDRVILLDGKGGACFSFINRLGEGQIEFELEEPLIDNKEAPIRITLAQSLIKKDNFEFVLQKGTELGATEF
YPLISQRTEVNLLNKSDKKVSKKLTRWREIVKNAAEQCQRQVLPLIHYPVNFEKYLKNNNENSNNNGSRLKIMCHEQENT
PLKKVLKTIETPPSSAIILIGPEGGFSHNEVDLAREQGFSSVSMGPRILRAETAGIVAVSAILYELADLGGTEQCQTYQG
>Mature_239_residues
TRVLRLKIGDRVILLDGKGGACFSFINRLGEGQIEFELEEPLIDNKEAPIRITLAQSLIKKDNFEFVLQKGTELGATEFY
PLISQRTEVNLLNKSDKKVSKKLTRWREIVKNAAEQCQRQVLPLIHYPVNFEKYLKNNNENSNNNGSRLKIMCHEQENTP
LKKVLKTIETPPSSAIILIGPEGGFSHNEVDLAREQGFSSVSMGPRILRAETAGIVAVSAILYELADLGGTEQCQTYQG

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=228, Percent_Identity=31.5789473684211, Blast_Score=124, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001206
- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26887; Mature: 26756

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRVLRLKIGDRVILLDGKGGACFSFINRLGEGQIEFELEEPLIDNKEAPIRITLAQSLI
CCEEEEEEECCEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEHHHHHHH
KKDNFEFVLQKGTELGATEFYPLISQRTEVNLLNKSDKKVSKKLTRWREIVKNAAEQCQR
HCCCHHEEEECCCCCCCHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
QVLPLIHYPVNFEKYLKNNNENSNNNGSRLKIMCHEQENTPLKKVLKTIETPPSSAIILI
HCCCCEECCCCHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEE
GPEGGFSHNEVDLAREQGFSSVSMGPRILRAETAGIVAVSAILYELADLGGTEQCQTYQG
CCCCCCCCCHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCHHCCCCC
>Mature Secondary Structure 
TRVLRLKIGDRVILLDGKGGACFSFINRLGEGQIEFELEEPLIDNKEAPIRITLAQSLI
CEEEEEEECCEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEHHHHHHH
KKDNFEFVLQKGTELGATEFYPLISQRTEVNLLNKSDKKVSKKLTRWREIVKNAAEQCQR
HCCCHHEEEECCCCCCCHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
QVLPLIHYPVNFEKYLKNNNENSNNNGSRLKIMCHEQENTPLKKVLKTIETPPSSAIILI
HCCCCEECCCCHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEE
GPEGGFSHNEVDLAREQGFSSVSMGPRILRAETAGIVAVSAILYELADLGGTEQCQTYQG
CCCCCCCCCHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]