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The map label for this gene is grpE [H]

Identifier: 188585808

GI number: 188585808

Start: 1253664

End: 1254329

Strand: Direct

Name: grpE [H]

Synonym: Nther_1182

Alternate gene names: 188585808

Gene position: 1253664-1254329 (Clockwise)

Preceding gene: 188585807

Following gene: 188585809

Centisome position: 39.6

GC content: 36.49

Gene sequence:

>666_bases
ATGATTATGAAAAAAGAAAATAATCAAAATATCACAGAAGAACAAAAGCAATCGGCCAACCAAAAGAAAGAACAAAATCA
AAAAGATACAGAAACAACAAACACACTGGAAAATCACAAAGAGGAAGTAGAAAGCCGAGAAGAATCAGGTGAAGCAGAAG
ACTTACAATACCAGGGACAAGGGGAGGAGAATTTGGAGAAAAAAATTGAGGATTTAGAAAACCAGAACGAGCGTTTAGAG
GAGGAAAAACAGAGCTATCTCCAGCAATTGAAACGTTTACAAGCAGATTTTGATAATTATAAAAAGCGTACTGCAAAAGA
ATGGGAACGCACAAGCACAGAAAAAGCAAAAGAATTGGCAGAGGATATATTACCAATTTTGGACAATTTTGAAAGGGCTC
TTAACAACATTGACGACGAAAAAGTTGACCCCAATTTCTATGAAGGGGTTAATATGATTTATGATCAATTATATGAGGTA
CTAACCAAAAATGGGTTAGAGCGAATTGAAGCAGAAGGCCAAGAATTTGACCCTAATTATCACGAAGCGGTTATGCAAGT
TGACAGTGAGGAACATGAAAGCAATGTTGTCATTGAGGAGATTCAACCTGGCTTTCTATTTAAAGATCGCCTGCTGAGAG
CAAGTGTAGTAAAAGTTTCCAGATAA

Upstream 100 bases:

>100_bases
TACCAGGATGGATTACTCCAGGGTGATTGCCATAGTGGCTCAAATTGCCAATGAACTTACAATGGCATTGGATAAAAAGA
AAATTACTGAGGAGTAGGTG

Downstream 100 bases:

>100_bases
TAGATAATCTGGATTCAAAATGATTTGAACTATTCTATTCAAAACAAAACTTAAGGGAGGTAATAGTTATGGGTAAAATA
ATTGGAATCGATCTGGGAAC

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MIMKKENNQNITEEQKQSANQKKEQNQKDTETTNTLENHKEEVESREESGEAEDLQYQGQGEENLEKKIEDLENQNERLE
EEKQSYLQQLKRLQADFDNYKKRTAKEWERTSTEKAKELAEDILPILDNFERALNNIDDEKVDPNFYEGVNMIYDQLYEV
LTKNGLERIEAEGQEFDPNYHEAVMQVDSEEHESNVVIEEIQPGFLFKDRLLRASVVKVSR

Sequences:

>Translated_221_residues
MIMKKENNQNITEEQKQSANQKKEQNQKDTETTNTLENHKEEVESREESGEAEDLQYQGQGEENLEKKIEDLENQNERLE
EEKQSYLQQLKRLQADFDNYKKRTAKEWERTSTEKAKELAEDILPILDNFERALNNIDDEKVDPNFYEGVNMIYDQLYEV
LTKNGLERIEAEGQEFDPNYHEAVMQVDSEEHESNVVIEEIQPGFLFKDRLLRASVVKVSR
>Mature_221_residues
MIMKKENNQNITEEQKQSANQKKEQNQKDTETTNTLENHKEEVESREESGEAEDLQYQGQGEENLEKKIEDLENQNERLE
EEKQSYLQQLKRLQADFDNYKKRTAKEWERTSTEKAKELAEDILPILDNFERALNNIDDEKVDPNFYEGVNMIYDQLYEV
LTKNGLERIEAEGQEFDPNYHEAVMQVDSEEHESNVVIEEIQPGFLFKDRLLRASVVKVSR

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=150, Percent_Identity=30, Blast_Score=71, Evalue=7e-13,
Organism=Escherichia coli, GI1788967, Length=207, Percent_Identity=26.0869565217391, Blast_Score=74, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6324806, Length=156, Percent_Identity=31.4102564102564, Blast_Score=79, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24653432, Length=158, Percent_Identity=27.8481012658228, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 26122; Mature: 26122

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMKKENNQNITEEQKQSANQKKEQNQKDTETTNTLENHKEEVESREESGEAEDLQYQGQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCC
GEENLEKKIEDLENQNERLEEEKQSYLQQLKRLQADFDNYKKRTAKEWERTSTEKAKELA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDILPILDNFERALNNIDDEKVDPNFYEGVNMIYDQLYEVLTKNGLERIEAEGQEFDPNY
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
HEAVMQVDSEEHESNVVIEEIQPGFLFKDRLLRASVVKVSR
HHHHHHHCCHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIMKKENNQNITEEQKQSANQKKEQNQKDTETTNTLENHKEEVESREESGEAEDLQYQGQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCC
GEENLEKKIEDLENQNERLEEEKQSYLQQLKRLQADFDNYKKRTAKEWERTSTEKAKELA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDILPILDNFERALNNIDDEKVDPNFYEGVNMIYDQLYEVLTKNGLERIEAEGQEFDPNY
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
HEAVMQVDSEEHESNVVIEEIQPGFLFKDRLLRASVVKVSR
HHHHHHHCCHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]