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The map label for this gene is kipI [H]

Identifier: 188585771

GI number: 188585771

Start: 1210279

End: 1210968

Strand: Direct

Name: kipI [H]

Synonym: Nther_1145

Alternate gene names: 188585771

Gene position: 1210279-1210968 (Clockwise)

Preceding gene: 188585770

Following gene: 188585772

Centisome position: 38.23

GC content: 35.8

Gene sequence:

>690_bases
ATGTCAAAATTACAGATATTACCTGCCGGGGATAAAGCTGTTGTTATGGAGTTCGAAAATGAGATTTCACCAGAAGTCAA
TGCTAAAATAAGAGCAACGGATCATTTGTTGAGAGAGGAGTTTCAATTAAAAGGCATTACAGAAATAATACCAACTTATA
GATCATTACTAATATACTACGATGAAAAATACTGGGATTTTGAAACCCTGTCAAAAAAATTAGTTGAAATACCTCATTAT
CTGGAAGAAAAGGAGATTCCTGAGCCAAAAACTCTAAATATACCTGTTGTTTATGGTGGTGAATACGGGCCAGATTTAGA
ATATGTAGCGGAGTACAACAATCTAACTTCAGATCAGGTGATAGAAATACATCATACAACAACTTATTTAATTTATATGC
TAGGTTTTGTTCCTGGGTTCACCTATTTAGGTGGAATGTCGGAGAAAATAGCTACACCCCGATTAAAAGACCCCAGGGCT
AAAATTCCTGAAGGTTCAGTAGGCATTGCTGGTAGTCAGACTGGTGTATATCCCTTAGAAAGTCCAGGAGGATGGCAACT
AATAGGACGCACACCATTAAAGTTTTATGATCCTAACAAAGAAGATCCTTTCCTGCCGAAACCAGGATATCACTTAAAGT
TTTATCCTATTACTGAAGATGAATATCGAGAATTTAAAAATAAGTATTAG

Upstream 100 bases:

>100_bases
TGCTCTTACAGGGGTACTTCTGTAAGCTACTATTATTATAACTTGAGAATTCAAAGGGCCCCTGACAAGTTTAGGGGCCT
TATTAATGTGGAGGGATAGC

Downstream 100 bases:

>100_bases
CCGGATTCAACTAATCTCGGATAGCTTTATAAAATATTTTTTATAAGTGCTGTATTAAAGTTGAAAATTTTACAGTATCA
GCAACTAAATAAACAGTGCC

Product: Allophanate hydrolase subunit 1

Products: CO2; NH3

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MSKLQILPAGDKAVVMEFENEISPEVNAKIRATDHLLREEFQLKGITEIIPTYRSLLIYYDEKYWDFETLSKKLVEIPHY
LEEKEIPEPKTLNIPVVYGGEYGPDLEYVAEYNNLTSDQVIEIHHTTTYLIYMLGFVPGFTYLGGMSEKIATPRLKDPRA
KIPEGSVGIAGSQTGVYPLESPGGWQLIGRTPLKFYDPNKEDPFLPKPGYHLKFYPITEDEYREFKNKY

Sequences:

>Translated_229_residues
MSKLQILPAGDKAVVMEFENEISPEVNAKIRATDHLLREEFQLKGITEIIPTYRSLLIYYDEKYWDFETLSKKLVEIPHY
LEEKEIPEPKTLNIPVVYGGEYGPDLEYVAEYNNLTSDQVIEIHHTTTYLIYMLGFVPGFTYLGGMSEKIATPRLKDPRA
KIPEGSVGIAGSQTGVYPLESPGGWQLIGRTPLKFYDPNKEDPFLPKPGYHLKFYPITEDEYREFKNKY
>Mature_228_residues
SKLQILPAGDKAVVMEFENEISPEVNAKIRATDHLLREEFQLKGITEIIPTYRSLLIYYDEKYWDFETLSKKLVEIPHYL
EEKEIPEPKTLNIPVVYGGEYGPDLEYVAEYNNLTSDQVIEIHHTTTYLIYMLGFVPGFTYLGGMSEKIATPRLKDPRAK
IPEGSVGIAGSQTGVYPLESPGGWQLIGRTPLKFYDPNKEDPFLPKPGYHLKFYPITEDEYREFKNKY

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=222, Percent_Identity=39.1891891891892, Blast_Score=164, Evalue=6e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: 3.5.1.54

Molecular weight: Translated: 26378; Mature: 26247

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLQILPAGDKAVVMEFENEISPEVNAKIRATDHLLREEFQLKGITEIIPTYRSLLIYY
CCCEEEEECCCCEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHEEEEE
DEKYWDFETLSKKLVEIPHYLEEKEIPEPKTLNIPVVYGGEYGPDLEYVAEYNNLTSDQV
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCE
IEIHHTTTYLIYMLGFVPGFTYLGGMSEKIATPRLKDPRAKIPEGSVGIAGSQTGVYPLE
EEEEHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCEECCCCCCCCEECC
SPGGWQLIGRTPLKFYDPNKEDPFLPKPGYHLKFYPITEDEYREFKNKY
CCCCEEEECCCCCEEECCCCCCCCCCCCCCEEEEEECCHHHHHHHHCCC
>Mature Secondary Structure 
SKLQILPAGDKAVVMEFENEISPEVNAKIRATDHLLREEFQLKGITEIIPTYRSLLIYY
CCEEEEECCCCEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHEEEEE
DEKYWDFETLSKKLVEIPHYLEEKEIPEPKTLNIPVVYGGEYGPDLEYVAEYNNLTSDQV
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCE
IEIHHTTTYLIYMLGFVPGFTYLGGMSEKIATPRLKDPRAKIPEGSVGIAGSQTGVYPLE
EEEEHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCEECCCCCCCCEECC
SPGGWQLIGRTPLKFYDPNKEDPFLPKPGYHLKFYPITEDEYREFKNKY
CCCCEEEECCCCCEEECCCCCCCCCCCCCCEEEEEECCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: urea-1-carboxylate; H2O

Specific reaction: urea-1-carboxylate + H2O = 2 CO2 + 2 NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]