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The map label for this gene is folC [H]

Identifier: 188585736

GI number: 188585736

Start: 1175731

End: 1177128

Strand: Direct

Name: folC [H]

Synonym: Nther_1110

Alternate gene names: 188585736

Gene position: 1175731-1177128 (Clockwise)

Preceding gene: 188585735

Following gene: 188585738

Centisome position: 37.14

GC content: 37.84

Gene sequence:

>1398_bases
GTGGCTATTAGTGGTGATGAAGCTATTAAATGGATCCAAAATCTCGACAAATTCGGTTCTAAACCCGGACTGGAGAGAAT
TAACTGGCTGTTAACTGAATTAAATCACCCTCATAATCACTATCCTGTCATTCACGTGGCAGGAACTAATGGGAAAGGTT
CTACCTGCTCCATACTAGAAAGTCTTCTTTCTAGTATGGGGTATCAGGTAGGTCTGTTCACCTCTCCTTTTATTGATGAT
TTTGCTGAACGAGTCACTTGTAATTCTAAACCAATTACTAAAACTGAACTGGGTGAATTGGTCTGCGAAATAAAACCTAT
AGTAGAAGCGGCTGAAGCCAAAGGTTTAGGACACCCAACCCAATTCGAGGTTATTACAGCTTTGGCACTGCTTTATTTTC
AAAAAAAGTCGCCTGATATGGTAATCCTTGAAACAGGCTTAGGAGGTAGATTGGATGCAACCAATGTAGTAACCCCAGTA
CTTTCTATTATCACTAGTATAGGTTACGACCATATTAATGTTTTGGGAAACACCCTTACAAAAATAGCCGAAGAAAAAGC
TGGAATTATCAAAGAGAATATACCGGTGATTACTGGACTACATCAGGATAAAGCTAGTATTGAAGAAGATACACAGGAGC
AAGCATTTTCAGTAATTGAGGAAAAGTGCCAGAAGAAAGGGTCTCCTTTATATGCCAGCGAACGGAACTGGAATTATCGA
ATAAAAGCTTTTTCATCCACCGGTTATTGCTTTGATTATTTTGGTTTAGAAGCTGAATTTATTAATCTTAAGTTACCATT
ACTCGGGGAATCACAGATAAAAAATGCTTCTATGGCTTTATGTGCCTTAGAATTATTAAAAAAACAAGGTTGGAATGAGA
TTGATTGGAATGATATGAGAAAAGGAATGGTTAGGGTCAATATACCAGCTAGAATGGAATTTTGGCCTGCAGATTCCGGG
GAAAAAACTATTACCGGTTCTAGAGCTGAAAAACAACCAGATGTGTTACTAGATGCTTCGCATAATCCTCCAAGTGTCCA
AAATTTGATGAGCTGTATAAGACGTTTGGATACCAATAGGAAATGGAATAAAATAGTGTTTGTTACTGGAGTATTACAAG
ATAAACCGATGGAAAAAATAGTTGCCAACTTCAGCTTGATCCCTGATGAGATTGTTGTAACAGAACCCCAAAATACTAGA
GCCCTTTCAGCTCATTGTTTGCTGGACTATATTACTGATCAATTACCAGATACCAATATTTCTGTGGAAAGTGATCTTTA
CAAGGCAGTTCGAATGGGAATCTCAAAAGCTGGCAACGGAAGTTTGTTAGTGATCTTTGGTTCTTTTTACTTAGTAGTAG
AAGCTCGGCGATATTTGAAGACAGATATACTTGCTTAA

Upstream 100 bases:

>100_bases
GTTGACAAGGAGCGAGAAAAGGAAAGAGACTATAAAGAAAGGTTAGCTAAAGTTCAGGAACGTCTTGATACTTTATTAGC
AATGAAGGAGTGAGCAGTCG

Downstream 100 bases:

>100_bases
ATTTTTAGCCATTTTAATTTTGAAGGTGAAACTCTTGTATCCACGATTTTTAAATATTCTTCAGTAGTTGAACGAAAGCG
ATGACCGAGAAGTTCTTGAA

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MAISGDEAIKWIQNLDKFGSKPGLERINWLLTELNHPHNHYPVIHVAGTNGKGSTCSILESLLSSMGYQVGLFTSPFIDD
FAERVTCNSKPITKTELGELVCEIKPIVEAAEAKGLGHPTQFEVITALALLYFQKKSPDMVILETGLGGRLDATNVVTPV
LSIITSIGYDHINVLGNTLTKIAEEKAGIIKENIPVITGLHQDKASIEEDTQEQAFSVIEEKCQKKGSPLYASERNWNYR
IKAFSSTGYCFDYFGLEAEFINLKLPLLGESQIKNASMALCALELLKKQGWNEIDWNDMRKGMVRVNIPARMEFWPADSG
EKTITGSRAEKQPDVLLDASHNPPSVQNLMSCIRRLDTNRKWNKIVFVTGVLQDKPMEKIVANFSLIPDEIVVTEPQNTR
ALSAHCLLDYITDQLPDTNISVESDLYKAVRMGISKAGNGSLLVIFGSFYLVVEARRYLKTDILA

Sequences:

>Translated_465_residues
MAISGDEAIKWIQNLDKFGSKPGLERINWLLTELNHPHNHYPVIHVAGTNGKGSTCSILESLLSSMGYQVGLFTSPFIDD
FAERVTCNSKPITKTELGELVCEIKPIVEAAEAKGLGHPTQFEVITALALLYFQKKSPDMVILETGLGGRLDATNVVTPV
LSIITSIGYDHINVLGNTLTKIAEEKAGIIKENIPVITGLHQDKASIEEDTQEQAFSVIEEKCQKKGSPLYASERNWNYR
IKAFSSTGYCFDYFGLEAEFINLKLPLLGESQIKNASMALCALELLKKQGWNEIDWNDMRKGMVRVNIPARMEFWPADSG
EKTITGSRAEKQPDVLLDASHNPPSVQNLMSCIRRLDTNRKWNKIVFVTGVLQDKPMEKIVANFSLIPDEIVVTEPQNTR
ALSAHCLLDYITDQLPDTNISVESDLYKAVRMGISKAGNGSLLVIFGSFYLVVEARRYLKTDILA
>Mature_464_residues
AISGDEAIKWIQNLDKFGSKPGLERINWLLTELNHPHNHYPVIHVAGTNGKGSTCSILESLLSSMGYQVGLFTSPFIDDF
AERVTCNSKPITKTELGELVCEIKPIVEAAEAKGLGHPTQFEVITALALLYFQKKSPDMVILETGLGGRLDATNVVTPVL
SIITSIGYDHINVLGNTLTKIAEEKAGIIKENIPVITGLHQDKASIEEDTQEQAFSVIEEKCQKKGSPLYASERNWNYRI
KAFSSTGYCFDYFGLEAEFINLKLPLLGESQIKNASMALCALELLKKQGWNEIDWNDMRKGMVRVNIPARMEFWPADSGE
KTITGSRAEKQPDVLLDASHNPPSVQNLMSCIRRLDTNRKWNKIVFVTGVLQDKPMEKIVANFSLIPDEIVVTEPQNTRA
LSAHCLLDYITDQLPDTNISVESDLYKAVRMGISKAGNGSLLVIFGSFYLVVEARRYLKTDILA

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=326, Percent_Identity=28.8343558282209, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI66932990, Length=326, Percent_Identity=28.8343558282209, Blast_Score=125, Evalue=6e-29,
Organism=Escherichia coli, GI1788654, Length=456, Percent_Identity=28.7280701754386, Blast_Score=164, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17553150, Length=315, Percent_Identity=27.9365079365079, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17553148, Length=315, Percent_Identity=27.9365079365079, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71984923, Length=315, Percent_Identity=27.9365079365079, Blast_Score=123, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323760, Length=448, Percent_Identity=30.1339285714286, Blast_Score=182, Evalue=8e-47,
Organism=Saccharomyces cerevisiae, GI6324815, Length=261, Percent_Identity=27.2030651340996, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322718, Length=327, Percent_Identity=25.3822629969419, Blast_Score=90, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24641571, Length=210, Percent_Identity=34.7619047619048, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24581568, Length=359, Percent_Identity=25.3481894150418, Blast_Score=99, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 51666; Mature: 51535

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISGDEAIKWIQNLDKFGSKPGLERINWLLTELNHPHNHYPVIHVAGTNGKGSTCSILE
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHH
SLLSSMGYQVGLFTSPFIDDFAERVTCNSKPITKTELGELVCEIKPIVEAAEAKGLGHPT
HHHHHCCCEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCH
QFEVITALALLYFQKKSPDMVILETGLGGRLDATNVVTPVLSIITSIGYDHINVLGNTLT
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
KIAEEKAGIIKENIPVITGLHQDKASIEEDTQEQAFSVIEEKCQKKGSPLYASERNWNYR
HHHHHHCCHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEE
IKAFSSTGYCFDYFGLEAEFINLKLPLLGESQIKNASMALCALELLKKQGWNEIDWNDMR
EEEECCCCCEEEECCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHC
KGMVRVNIPARMEFWPADSGEKTITGSRAEKQPDVLLDASHNPPSVQNLMSCIRRLDTNR
CCEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCC
KWNKIVFVTGVLQDKPMEKIVANFSLIPDEIVVTEPQNTRALSAHCLLDYITDQLPDTNI
CCCEEEEEEECCCCCCHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCC
SVESDLYKAVRMGISKAGNGSLLVIFGSFYLVVEARRYLKTDILA
CHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AISGDEAIKWIQNLDKFGSKPGLERINWLLTELNHPHNHYPVIHVAGTNGKGSTCSILE
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHH
SLLSSMGYQVGLFTSPFIDDFAERVTCNSKPITKTELGELVCEIKPIVEAAEAKGLGHPT
HHHHHCCCEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCH
QFEVITALALLYFQKKSPDMVILETGLGGRLDATNVVTPVLSIITSIGYDHINVLGNTLT
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
KIAEEKAGIIKENIPVITGLHQDKASIEEDTQEQAFSVIEEKCQKKGSPLYASERNWNYR
HHHHHHCCHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEE
IKAFSSTGYCFDYFGLEAEFINLKLPLLGESQIKNASMALCALELLKKQGWNEIDWNDMR
EEEECCCCCEEEECCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHC
KGMVRVNIPARMEFWPADSGEKTITGSRAEKQPDVLLDASHNPPSVQNLMSCIRRLDTNR
CCEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCC
KWNKIVFVTGVLQDKPMEKIVANFSLIPDEIVVTEPQNTRALSAHCLLDYITDQLPDTNI
CCCEEEEEEECCCCCCHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCC
SVESDLYKAVRMGISKAGNGSLLVIFGSFYLVVEARRYLKTDILA
CHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]