Click here to switch to the map view.

The map label for this gene is dusB [H]

Identifier: 188584754

GI number: 188584754

Start: 129394

End: 130377

Strand: Direct

Name: dusB [H]

Synonym: Nther_0112

Alternate gene names: 188584754

Gene position: 129394-130377 (Clockwise)

Preceding gene: 188584753

Following gene: 188584755

Centisome position: 4.09

GC content: 38.62

Gene sequence:

>984_bases
ATGAACAAGATGAAGTTAGGAAAGCTAACTTTAGATAATTGCATCATTGTTGCACCTATGGCAGGAGTGACCGATTACCC
TTATCGACGAATTTTGGCAAAATTTTCACCAGGCTTGATATGTGGTGAGATGGTGAGTGCCAAGGGAATGGTGTATGGCA
ACAAAAAAACTGAAGAAATGGTTATTCATCACAATCAAGGAGTGCATTATTCTCAACAGATATTTGGCAGTGATCCCAAG
GTAATGGCAGATGCCGCAGTGAAACTGGAGAATTTAGGAGTTGACTCCATTGATGTAAATATGGGTTGTCCAGCTCCCAA
AATAGTCAAAAATGGAGAGGGTTCAGCTCTGCTAAAGAAACCTCATTTAGCTCAAGAAATCGTCAGAGAAATAGTCAATT
CAGTAAACCTTCCTGTGACGGTAAAGATTAGAAAGGGATTTGACAGAAAGTCGTCTGACCCACTGGAATTAGCTCTTAGA
TTGCAGGAAGTAGGAATCAAGGCTCTGGCAGTTCATGGGAGGACCTGTGAACAGCAATATAGTGGCAAAGCGGATTGGGA
AGTGGTTGCTAAACTTAAAAACTATTTAGATATACCTGTTATCGGAAATGGAGATATTTTCAGCTCTGAAGATGCTGTGT
CTGCTTTGGAGATGACTGCTTGTGATGGAATTATGTTGGGCCGTGGACTAATGGGAAATCCTTGGCTGATTGAACAGACC
AAAGGAATGCTTATCAAAGATGAGAAAATTGAGTCTCCAGAAATTCATGAGATTAAAAATGAGATGAAAAGTCATTTTGA
AAATGCTATTAAATATTACGGTGAGTCAAAAGGCATAGCTGAAATGAGAAAGCATATTTCATGGTATCTAAAAAGTCTTC
CTGGAAGTACGAGCATCAAAGATGAAGTCATGCGGCTAACTGATGTAAACCAAATTTGGTTTAGTTTAAATGAATATTTT
CAAGCTTTAGAAAAAAAGAGTTAA

Upstream 100 bases:

>100_bases
ACATGTGTGAAATTGTCAATATCAAAAGTGAAGAACAGTTATTGGCTCGATGGGGTGGGCGATGTGAAAAATGGGAAGTT
TTAGAAACGAATGTCAACAC

Downstream 100 bases:

>100_bases
AAATTACATCATTAACATAAAGCCAGGTCCCAGGTCAAAGAACGGTTATCGTCTTGACCTAGGACCTGGCTTTTTAAGTA
TCTTGAATTTTATAAGAAAA

Product: TIM-barrel protein, nifR3 family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MNKMKLGKLTLDNCIIVAPMAGVTDYPYRRILAKFSPGLICGEMVSAKGMVYGNKKTEEMVIHHNQGVHYSQQIFGSDPK
VMADAAVKLENLGVDSIDVNMGCPAPKIVKNGEGSALLKKPHLAQEIVREIVNSVNLPVTVKIRKGFDRKSSDPLELALR
LQEVGIKALAVHGRTCEQQYSGKADWEVVAKLKNYLDIPVIGNGDIFSSEDAVSALEMTACDGIMLGRGLMGNPWLIEQT
KGMLIKDEKIESPEIHEIKNEMKSHFENAIKYYGESKGIAEMRKHISWYLKSLPGSTSIKDEVMRLTDVNQIWFSLNEYF
QALEKKS

Sequences:

>Translated_327_residues
MNKMKLGKLTLDNCIIVAPMAGVTDYPYRRILAKFSPGLICGEMVSAKGMVYGNKKTEEMVIHHNQGVHYSQQIFGSDPK
VMADAAVKLENLGVDSIDVNMGCPAPKIVKNGEGSALLKKPHLAQEIVREIVNSVNLPVTVKIRKGFDRKSSDPLELALR
LQEVGIKALAVHGRTCEQQYSGKADWEVVAKLKNYLDIPVIGNGDIFSSEDAVSALEMTACDGIMLGRGLMGNPWLIEQT
KGMLIKDEKIESPEIHEIKNEMKSHFENAIKYYGESKGIAEMRKHISWYLKSLPGSTSIKDEVMRLTDVNQIWFSLNEYF
QALEKKS
>Mature_327_residues
MNKMKLGKLTLDNCIIVAPMAGVTDYPYRRILAKFSPGLICGEMVSAKGMVYGNKKTEEMVIHHNQGVHYSQQIFGSDPK
VMADAAVKLENLGVDSIDVNMGCPAPKIVKNGEGSALLKKPHLAQEIVREIVNSVNLPVTVKIRKGFDRKSSDPLELALR
LQEVGIKALAVHGRTCEQQYSGKADWEVVAKLKNYLDIPVIGNGDIFSSEDAVSALEMTACDGIMLGRGLMGNPWLIEQT
KGMLIKDEKIESPEIHEIKNEMKSHFENAIKYYGESKGIAEMRKHISWYLKSLPGSTSIKDEVMRLTDVNQIWFSLNEYF
QALEKKS

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=282, Percent_Identity=30.1418439716312, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI239788462, Length=289, Percent_Identity=29.757785467128, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI31742496, Length=222, Percent_Identity=30.1801801801802, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI8923374, Length=245, Percent_Identity=30.6122448979592, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI40807366, Length=228, Percent_Identity=30.2631578947368, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1789660, Length=318, Percent_Identity=35.5345911949685, Blast_Score=218, Evalue=3e-58,
Organism=Escherichia coli, GI1788462, Length=230, Percent_Identity=31.304347826087, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI145693211, Length=296, Percent_Identity=25, Blast_Score=75, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17543114, Length=296, Percent_Identity=27.7027027027027, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25144369, Length=222, Percent_Identity=28.8288288288288, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17507177, Length=243, Percent_Identity=27.1604938271605, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17510279, Length=254, Percent_Identity=25.5905511811024, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323433, Length=309, Percent_Identity=27.1844660194175, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323560, Length=273, Percent_Identity=29.6703296703297, Blast_Score=90, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=227, Percent_Identity=23.7885462555066, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324342, Length=141, Percent_Identity=34.0425531914894, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24585320, Length=282, Percent_Identity=32.9787234042553, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24580595, Length=226, Percent_Identity=29.646017699115, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19920448, Length=226, Percent_Identity=29.646017699115, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19921524, Length=239, Percent_Identity=28.0334728033473, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI45549423, Length=262, Percent_Identity=27.0992366412214, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36400; Mature: 36400

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKMKLGKLTLDNCIIVAPMAGVTDYPYRRILAKFSPGLICGEMVSAKGMVYGNKKTEEM
CCCCCCCCEEECCEEEEECCCCCCCCHHHHHHHHCCCCCEEHHHHHCCCEEECCCCCCEE
VIHHNQGVHYSQQIFGSDPKVMADAAVKLENLGVDSIDVNMGCPAPKIVKNGEGSALLKK
EEECCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHEECCCCCEEECC
PHLAQEIVREIVNSVNLPVTVKIRKGFDRKSSDPLELALRLQEVGIKALAVHGRTCEQQY
CHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCEEEEEECCCCHHHHH
SGKADWEVVAKLKNYLDIPVIGNGDIFSSEDAVSALEMTACDGIMLGRGLMGNPWLIEQT
CCCCHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHCCCHHCCCCCCCCCEEEECC
KGMLIKDEKIESPEIHEIKNEMKSHFENAIKYYGESKGIAEMRKHISWYLKSLPGSTSIK
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHH
DEVMRLTDVNQIWFSLNEYFQALEKKS
HHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKMKLGKLTLDNCIIVAPMAGVTDYPYRRILAKFSPGLICGEMVSAKGMVYGNKKTEEM
CCCCCCCCEEECCEEEEECCCCCCCCHHHHHHHHCCCCCEEHHHHHCCCEEECCCCCCEE
VIHHNQGVHYSQQIFGSDPKVMADAAVKLENLGVDSIDVNMGCPAPKIVKNGEGSALLKK
EEECCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHEECCCCCEEECC
PHLAQEIVREIVNSVNLPVTVKIRKGFDRKSSDPLELALRLQEVGIKALAVHGRTCEQQY
CHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCEEEEEECCCCHHHHH
SGKADWEVVAKLKNYLDIPVIGNGDIFSSEDAVSALEMTACDGIMLGRGLMGNPWLIEQT
CCCCHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHCCCHHCCCCCCCCCEEEECC
KGMLIKDEKIESPEIHEIKNEMKSHFENAIKYYGESKGIAEMRKHISWYLKSLPGSTSIK
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHH
DEVMRLTDVNQIWFSLNEYFQALEKKS
HHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]