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The map label for this gene is nasB [H]

Identifier: 188584744

GI number: 188584744

Start: 118252

End: 119451

Strand: Direct

Name: nasB [H]

Synonym: Nther_0102

Alternate gene names: 188584744

Gene position: 118252-119451 (Clockwise)

Preceding gene: 188584743

Following gene: 188584745

Centisome position: 3.74

GC content: 35.42

Gene sequence:

>1200_bases
TTGCATTACGTAATAGTAGGTAGTGGTGTTTCAGGAATTACGGCAGCTAAAAATCTGAGAAATTTAAAACCTGAGGCCAA
AATTACTATTCTTGGAGATGAGCCACATTACTGTTATAATCGACCTAGATTAATAGAACTTTTGGGAAAAGAGGTTGACA
TCGAAAATATTTTCTTTTATCCTCCTGATTGGTATTGTCAACAGGGAATTGATATTTATCTCAATGTAACAGTGAAAGAA
ATTCATAGTAAAAAACAGCTAGTTATTACCGATTGTGGTCAGGAAATTTCTTATGATAAATTATTGCTTGCCAATGGAAG
TACCCCGAATCTTCCACCTGTCCCAGGGGCAGAAAATCGTGGTGTGTTCACTATTAGAACCATTGATGAGGTTAGGCAAA
TACAAAGAAGAGCTCTTACAAGTAGTCAAAGTATCGTTATTGGTGGGGGCTTATTGGGATTAGAAACGGCTTTTAGTTTA
ACTAAATTAAAGCAAGAAGTTACTGTGATTGATAGGAATTCGTGGCTTCTTAAAAGGCAAATTGATCAAAAAGGTAGTGA
ACTTCTGCAAGAAATTTTAAAGTCATACGGGATTTCAGTAGTTTTAGAAGGGAATACTGAAAGTATTGAACCGCCAAAAA
ATTCTGTAGGAGATTCCAAAGATTTAAATGTTAATTTGAAAGATGGTAGAAAAATACCAGGACAGATAGTTATTTTTTCC
GCAGGGGTACGCCCTAATATATCTCTAGCTGAAAAGGCTGGAATTTCAGTCAATAAAGGAATTGTAGTAGACGAACATTT
AAAGACAAGTGATCCAAATATTTACGCATCAGGAGATGTAGCAGAATTCCAAGATAAAAGTTATGGAATTATTCCAGCTG
CCTTAGAACAAGGCAAATTAGCAGCAGTAAACATGGCCAGTGAAGAGGGACAAAATTACGAAGGGACTATTCCTTCGACT
TCCTTAAAAATTGCAGGAATAGATCTGATCTCTCTCGGGGAAATTTATCCTCAAGAAGACGATCAGGTGGTGACCAAGAT
AGACTCCAGTAATAAAATTTTTAAAAAAGCTATCTTTAGAGAAAATTGTCTAAAGGGAGCCATTTTATTAGGAGATAAAA
CAGAACTTCCGCTAATTCACAAAAATTTGAGAGCACAACAACCATTTCGAGATCCAGAGTTAATTTTTGAAAACTATTAA

Upstream 100 bases:

>100_bases
TGAAGAACAATAAGTTTAAGGTGACTAAACTCTTGATGCGGGACCAATATGTTTGGGCCCGCATCTTTTATTCTGGTTTT
AATATACTGGGGGTGTCATT

Downstream 100 bases:

>100_bases
AAACTATTATGCAAGTATGTTTTTTAACTAACTATATTTAGGGGTGCTAGTAGGGGTGTGCCAAAAATGATACTGGTAAC
ACCCCTTTAATTGTTATTTG

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: nitrite; NADH; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MHYVIVGSGVSGITAAKNLRNLKPEAKITILGDEPHYCYNRPRLIELLGKEVDIENIFFYPPDWYCQQGIDIYLNVTVKE
IHSKKQLVITDCGQEISYDKLLLANGSTPNLPPVPGAENRGVFTIRTIDEVRQIQRRALTSSQSIVIGGGLLGLETAFSL
TKLKQEVTVIDRNSWLLKRQIDQKGSELLQEILKSYGISVVLEGNTESIEPPKNSVGDSKDLNVNLKDGRKIPGQIVIFS
AGVRPNISLAEKAGISVNKGIVVDEHLKTSDPNIYASGDVAEFQDKSYGIIPAALEQGKLAAVNMASEEGQNYEGTIPST
SLKIAGIDLISLGEIYPQEDDQVVTKIDSSNKIFKKAIFRENCLKGAILLGDKTELPLIHKNLRAQQPFRDPELIFENY

Sequences:

>Translated_399_residues
MHYVIVGSGVSGITAAKNLRNLKPEAKITILGDEPHYCYNRPRLIELLGKEVDIENIFFYPPDWYCQQGIDIYLNVTVKE
IHSKKQLVITDCGQEISYDKLLLANGSTPNLPPVPGAENRGVFTIRTIDEVRQIQRRALTSSQSIVIGGGLLGLETAFSL
TKLKQEVTVIDRNSWLLKRQIDQKGSELLQEILKSYGISVVLEGNTESIEPPKNSVGDSKDLNVNLKDGRKIPGQIVIFS
AGVRPNISLAEKAGISVNKGIVVDEHLKTSDPNIYASGDVAEFQDKSYGIIPAALEQGKLAAVNMASEEGQNYEGTIPST
SLKIAGIDLISLGEIYPQEDDQVVTKIDSSNKIFKKAIFRENCLKGAILLGDKTELPLIHKNLRAQQPFRDPELIFENY
>Mature_399_residues
MHYVIVGSGVSGITAAKNLRNLKPEAKITILGDEPHYCYNRPRLIELLGKEVDIENIFFYPPDWYCQQGIDIYLNVTVKE
IHSKKQLVITDCGQEISYDKLLLANGSTPNLPPVPGAENRGVFTIRTIDEVRQIQRRALTSSQSIVIGGGLLGLETAFSL
TKLKQEVTVIDRNSWLLKRQIDQKGSELLQEILKSYGISVVLEGNTESIEPPKNSVGDSKDLNVNLKDGRKIPGQIVIFS
AGVRPNISLAEKAGISVNKGIVVDEHLKTSDPNIYASGDVAEFQDKSYGIIPAALEQGKLAAVNMASEEGQNYEGTIPST
SLKIAGIDLISLGEIYPQEDDQVVTKIDSSNKIFKKAIFRENCLKGAILLGDKTELPLIHKNLRAQQPFRDPELIFENY

Specific function: Required for nitrate assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI21389617, Length=354, Percent_Identity=25.4237288135593, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI226437568, Length=354, Percent_Identity=25.4237288135593, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI65787454, Length=354, Percent_Identity=25.4237288135593, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI4757732, Length=332, Percent_Identity=25.6024096385542, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI22202629, Length=332, Percent_Identity=25.6024096385542, Blast_Score=89, Evalue=9e-18,
Organism=Escherichia coli, GI1789765, Length=379, Percent_Identity=29.8153034300792, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1789065, Length=323, Percent_Identity=27.5541795665635, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1788892, Length=282, Percent_Identity=29.0780141843972, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32564386, Length=374, Percent_Identity=25.668449197861, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24585130, Length=324, Percent_Identity=26.5432098765432, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24639257, Length=314, Percent_Identity=26.1146496815287, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI281359715, Length=314, Percent_Identity=25.796178343949, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI281359713, Length=314, Percent_Identity=25.796178343949, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24639250, Length=314, Percent_Identity=25.796178343949, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI18543267, Length=314, Percent_Identity=25.796178343949, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24639252, Length=314, Percent_Identity=25.796178343949, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI28573993, Length=349, Percent_Identity=23.7822349570201, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24581020, Length=349, Percent_Identity=23.7822349570201, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.7.1.4

Molecular weight: Translated: 44028; Mature: 44028

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYVIVGSGVSGITAAKNLRNLKPEAKITILGDEPHYCYNRPRLIELLGKEVDIENIFFY
CEEEEEECCCCCHHHHHHHHCCCCCCEEEEEECCCCHHCCCCHHHHHHCCCCCCCEEEEE
PPDWYCQQGIDIYLNVTVKEIHSKKQLVITDCGQEISYDKLLLANGSTPNLPPVPGAENR
CCCHHHCCCCEEEEEEEEEHHCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCC
GVFTIRTIDEVRQIQRRALTSSQSIVIGGGLLGLETAFSLTKLKQEVTVIDRNSWLLKRQ
CEEEEEEHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHEEEEECCCEEEHHH
IDQKGSELLQEILKSYGISVVLEGNTESIEPPKNSVGDSKDLNVNLKDGRKIPGQIVIFS
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEE
AGVRPNISLAEKAGISVNKGIVVDEHLKTSDPNIYASGDVAEFQDKSYGIIPAALEQGKL
CCCCCCCCHHHHCCCEECCCEEEECCCCCCCCCEEECCCHHHHCCCCCCEEHHHHCCCCE
AAVNMASEEGQNYEGTIPSTSLKIAGIDLISLGEIYPQEDDQVVTKIDSSNKIFKKAIFR
EEEEEHHHCCCCCCCCCCCCEEEEECEEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHH
ENCLKGAILLGDKTELPLIHKNLRAQQPFRDPELIFENY
HHHCCCEEEECCCCCCCHHHHHCCCCCCCCCHHHHCCCC
>Mature Secondary Structure
MHYVIVGSGVSGITAAKNLRNLKPEAKITILGDEPHYCYNRPRLIELLGKEVDIENIFFY
CEEEEEECCCCCHHHHHHHHCCCCCCEEEEEECCCCHHCCCCHHHHHHCCCCCCCEEEEE
PPDWYCQQGIDIYLNVTVKEIHSKKQLVITDCGQEISYDKLLLANGSTPNLPPVPGAENR
CCCHHHCCCCEEEEEEEEEHHCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCC
GVFTIRTIDEVRQIQRRALTSSQSIVIGGGLLGLETAFSLTKLKQEVTVIDRNSWLLKRQ
CEEEEEEHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHEEEEECCCEEEHHH
IDQKGSELLQEILKSYGISVVLEGNTESIEPPKNSVGDSKDLNVNLKDGRKIPGQIVIFS
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEE
AGVRPNISLAEKAGISVNKGIVVDEHLKTSDPNIYASGDVAEFQDKSYGIIPAALEQGKL
CCCCCCCCHHHHCCCEECCCEEEECCCCCCCCCEEECCCHHHHCCCCCCEEHHHHCCCCE
AAVNMASEEGQNYEGTIPSTSLKIAGIDLISLGEIYPQEDDQVVTKIDSSNKIFKKAIFR
EEEEEHHHCCCCCCCCCCCCEEEEECEEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHH
ENCLKGAILLGDKTELPLIHKNLRAQQPFRDPELIFENY
HHHCCCEEEECCCCCCCHHHHHCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD; NAD+; NADH; Siroheme [C]

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.4 {NO2-}} 0.005 {NO2-}} 0.016 {NADH}} 5.3 {hydroxylamine}} [C]

Substrates: ammonium hydroxide; NAD+; NADP+; H2O

Specific reaction: ammonium hydroxide + 3 NAD(P)+ + H2O = nitrite + 3 NAD(P)H + 3 H+

General reaction: Redox reaction [C]

Inhibitor: 1, 10-Phenanthroline; 2, 2'-Bipyridine; Arsenite; CN-; NAD+; NADP+; NaN3; p-Chloromercuribenzoate; SO32- [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]