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The map label for this gene is kinA [H]

Identifier: 188584718

GI number: 188584718

Start: 90085

End: 91368

Strand: Direct

Name: kinA [H]

Synonym: Nther_0076

Alternate gene names: 188584718

Gene position: 90085-91368 (Clockwise)

Preceding gene: 188584717

Following gene: 188584719

Centisome position: 2.85

GC content: 33.1

Gene sequence:

>1284_bases
ATGCGCTCTGTTTTTTTAAGGATTAATTCATTATTTGGGGGGCCTTCATTGAAAAGTCAAAGGCCGTATTTCCAAAAAGG
GAAGTACATAAATCAAATTGATACTAACAGAATCCTGGATTCTTTATTTGATGCAGTATTAGTTATTAACCACTCTGGAG
TAATAACTTATATTAATAAAGTTGTTGAACAAGTGCTGAAAATTGAAGGCGAGACTTTGGTTGGATATGATTACCGTGAA
CTTTTAAAGTACAGTTACACCTATGAGTTTGAAAATTTTTCAATTTTTGAAACTGCTTTATCAGAAAGGAAAGCAGTTCA
TGATAAAGAGTTACACCTAATTGATTCTGAGAATAATGAAGTGATTATTAAGGGTAACACTTATCCAATATATGAAGGCG
AAAATTTCTTAGGTGTTATAGGTGTTTTCAGAGACATTACACGTGAAAAGGAATACCAGGAACAAACAAAAAGATGGGAA
GAAAAATTTACTCAGGCAGAAAAGCTAGCTATTGTAGGTGAAATGGCGGCGGGGACCGCTCATGAAATAAAAAATCCTCT
TGCTACAATCAAAGGGTTTTTACAATTGTTAGAAAGTAATGAGTTACCAGAATCTAAGAGAAAAGAATATTTAGCTACTA
TCACAGATGAAGTTAACAGAATGGAGCAACTTGTAAAAGAGTTTTTGCATCTATCTAAACCTTCTAATCAAGAAGAACAT
GAAACAAATGAAACAGTGTCTTTGTGTAAAGTGTTCCAAAAGACTTTGATTTTTTTTAGCCCCCAATTTAAAAATAAAAA
AGTTATGTTAAATATTAAATGTCCAGGACACCCTATATATCTACGGGGGAAATCTGATAAATTACAACAAGTATTTATAA
ATCTGATAAAAAATTCTTTGGAAGCCATGGAAAAGATACCTCAGGATTGTAGGAAACTAACCATCAAGGTGGATATTTAT
AAATCAAATAATAAAGATGATAAAAATGAAAAAGTGATCTTAGAATTTATAGATACTGGCCCAGGGATTCCCAAAAATAT
TATGAATAAGCTGTTTTCCCCTTTTGCCACTACTAAGGTTAGAGGTACTGGCCTGGGATTGCCTGTCAGCATACAAATTA
TTCAGAATTTAGGAGGGTATCTAGTAGCAGAGAATAGCCCCTTGAGGGGTGCTTGTTTTCGTATTGAACTCCCTTTATAT
GATGTTGATGGAGTTAACAGTATATGTAGAAATGTTTATATACCATATTGTGAAAGGGTGGCTGATTATGACAAACCAGA
TTAA

Upstream 100 bases:

>100_bases
GTAAGCGTGGAAGTCGCGATACTAAAGCGTTTAATTAATTAACTAACCAGTAGCTATTACACAGTAATTAACAGTGATTA
AGTAAGAAACAGAGCGCAAC

Downstream 100 bases:

>100_bases
AGCTGCGATTGATATAGGAAGCAATTCAATTAGGCTGCTAATAGCTAGAGTCAATAAAAATGGACTAGAGGAGCTATATA
GGGATGTCCAAACTACTCGG

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: Stage II sporulation protein F; Stage II sporulation protein J [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MRSVFLRINSLFGGPSLKSQRPYFQKGKYINQIDTNRILDSLFDAVLVINHSGVITYINKVVEQVLKIEGETLVGYDYRE
LLKYSYTYEFENFSIFETALSERKAVHDKELHLIDSENNEVIIKGNTYPIYEGENFLGVIGVFRDITREKEYQEQTKRWE
EKFTQAEKLAIVGEMAAGTAHEIKNPLATIKGFLQLLESNELPESKRKEYLATITDEVNRMEQLVKEFLHLSKPSNQEEH
ETNETVSLCKVFQKTLIFFSPQFKNKKVMLNIKCPGHPIYLRGKSDKLQQVFINLIKNSLEAMEKIPQDCRKLTIKVDIY
KSNNKDDKNEKVILEFIDTGPGIPKNIMNKLFSPFATTKVRGTGLGLPVSIQIIQNLGGYLVAENSPLRGACFRIELPLY
DVDGVNSICRNVYIPYCERVADYDKPD

Sequences:

>Translated_427_residues
MRSVFLRINSLFGGPSLKSQRPYFQKGKYINQIDTNRILDSLFDAVLVINHSGVITYINKVVEQVLKIEGETLVGYDYRE
LLKYSYTYEFENFSIFETALSERKAVHDKELHLIDSENNEVIIKGNTYPIYEGENFLGVIGVFRDITREKEYQEQTKRWE
EKFTQAEKLAIVGEMAAGTAHEIKNPLATIKGFLQLLESNELPESKRKEYLATITDEVNRMEQLVKEFLHLSKPSNQEEH
ETNETVSLCKVFQKTLIFFSPQFKNKKVMLNIKCPGHPIYLRGKSDKLQQVFINLIKNSLEAMEKIPQDCRKLTIKVDIY
KSNNKDDKNEKVILEFIDTGPGIPKNIMNKLFSPFATTKVRGTGLGLPVSIQIIQNLGGYLVAENSPLRGACFRIELPLY
DVDGVNSICRNVYIPYCERVADYDKPD
>Mature_427_residues
MRSVFLRINSLFGGPSLKSQRPYFQKGKYINQIDTNRILDSLFDAVLVINHSGVITYINKVVEQVLKIEGETLVGYDYRE
LLKYSYTYEFENFSIFETALSERKAVHDKELHLIDSENNEVIIKGNTYPIYEGENFLGVIGVFRDITREKEYQEQTKRWE
EKFTQAEKLAIVGEMAAGTAHEIKNPLATIKGFLQLLESNELPESKRKEYLATITDEVNRMEQLVKEFLHLSKPSNQEEH
ETNETVSLCKVFQKTLIFFSPQFKNKKVMLNIKCPGHPIYLRGKSDKLQQVFINLIKNSLEAMEKIPQDCRKLTIKVDIY
KSNNKDDKNEKVILEFIDTGPGIPKNIMNKLFSPFATTKVRGTGLGLPVSIQIIQNLGGYLVAENSPLRGACFRIELPLY
DVDGVNSICRNVYIPYCERVADYDKPD

Specific function: Phosphorylates the sporulation-regulatory proteins spo0A and spo0F. It also autophosphorylates in the presence of ATP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=368, Percent_Identity=26.6304347826087, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1790300, Length=357, Percent_Identity=26.890756302521, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1790436, Length=250, Percent_Identity=26.8, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI87081816, Length=264, Percent_Identity=29.1666666666667, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788393, Length=235, Percent_Identity=27.6595744680851, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI145693157, Length=279, Percent_Identity=24.7311827956989, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI48994928, Length=267, Percent_Identity=23.9700374531835, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1790346, Length=227, Percent_Identity=26.8722466960352, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788713, Length=236, Percent_Identity=26.271186440678, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1786912, Length=244, Percent_Identity=24.1803278688525, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789149, Length=261, Percent_Identity=27.9693486590038, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR013655
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS; PF08447 PAS_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49079; Mature: 49079

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSVFLRINSLFGGPSLKSQRPYFQKGKYINQIDTNRILDSLFDAVLVINHSGVITYINK
CCEEEEEEEHHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHEEECCCCHHHHHHH
VVEQVLKIEGETLVGYDYRELLKYSYTYEFENFSIFETALSERKAVHDKELHLIDSENNE
HHHHHHHHCCCEEECCCHHHHHHHHHEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCE
VIIKGNTYPIYEGENFLGVIGVFRDITREKEYQEQTKRWEEKFTQAEKLAIVGEMAAGTA
EEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
HEIKNPLATIKGFLQLLESNELPESKRKEYLATITDEVNRMEQLVKEFLHLSKPSNQEEH
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
ETNETVSLCKVFQKTLIFFSPQFKNKKVMLNIKCPGHPIYLRGKSDKLQQVFINLIKNSL
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHH
EAMEKIPQDCRKLTIKVDIYKSNNKDDKNEKVILEFIDTGPGIPKNIMNKLFSPFATTKV
HHHHHHHHHHHEEEEEEEEEECCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCHHHCEE
RGTGLGLPVSIQIIQNLGGYLVAENSPLRGACFRIELPLYDVDGVNSICRNVYIPYCERV
CCCCCCCCCHHHHHHHCCCEEEECCCCCCCEEEEEEECEECCCCHHHHHHHCCCHHHHHH
ADYDKPD
CCCCCCC
>Mature Secondary Structure
MRSVFLRINSLFGGPSLKSQRPYFQKGKYINQIDTNRILDSLFDAVLVINHSGVITYINK
CCEEEEEEEHHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHEEECCCCHHHHHHH
VVEQVLKIEGETLVGYDYRELLKYSYTYEFENFSIFETALSERKAVHDKELHLIDSENNE
HHHHHHHHCCCEEECCCHHHHHHHHHEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCE
VIIKGNTYPIYEGENFLGVIGVFRDITREKEYQEQTKRWEEKFTQAEKLAIVGEMAAGTA
EEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
HEIKNPLATIKGFLQLLESNELPESKRKEYLATITDEVNRMEQLVKEFLHLSKPSNQEEH
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
ETNETVSLCKVFQKTLIFFSPQFKNKKVMLNIKCPGHPIYLRGKSDKLQQVFINLIKNSL
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHH
EAMEKIPQDCRKLTIKVDIYKSNNKDDKNEKVILEFIDTGPGIPKNIMNKLFSPFATTKV
HHHHHHHHHHHEEEEEEEEEECCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCHHHCEE
RGTGLGLPVSIQIIQNLGGYLVAENSPLRGACFRIELPLYDVDGVNSICRNVYIPYCERV
CCCCCCCCCHHHHHHHCCCEEEECCCCCCCEEEEEEECEECCCCHHHHHHHCCCHHHHHH
ADYDKPD
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2104615; 2509430; 9384377 [H]