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The map label for this gene is rsmA

Identifier: 188584688

GI number: 188584688

Start: 60730

End: 61638

Strand: Direct

Name: rsmA

Synonym: Nther_0046

Alternate gene names: 188584688

Gene position: 60730-61638 (Clockwise)

Preceding gene: 188584687

Following gene: 188584689

Centisome position: 1.92

GC content: 33.0

Gene sequence:

>909_bases
TTGAATTTATTTTCCCCTAAGGAAGTTTCACAACTACTTAAACGTTATGAGATTCACCCTAAAAAAAGCTTAGGCCAGAA
CTTTTTAGTTGACGGTAATATTATTCAAAAGATTATTGCTGCTGCTGAATTAAAGGAGCAAGATATAGTATTGGAAATAG
GCCCGGGTCTTGGAACACTGACCCGGGATATGTCTTTTTATGTTAATGAGATTTTTGCTATAGAACTGGACCAAAGAATG
ATAGATATTTTACAAGAGACCGTCGGTAGCTGTGATAACGTAAACATTATTCATAATGATGCATTGAAGCTAGATTATCA
AGAATTGATTTCTGATTTTATTGAGTTTAGTCCTGCACAGCTACAGTGCAAAAGTAAGCAAATTAATCCAAAAAATTTAA
AAGCAGTATCCAATTTACCTTATTATATTGCTTCTCCTCTAGTTTTGAAACTAGCCAAAGAAAAAGTTCCTCTTTCTGTA
ATGGTTTTAATGGTACAAAGGGAAGTTGCTGATAGGTTTACTGCCTCTCCGGGCAGTAAAAATTATGGAGCAGTAACGGT
TTTGTTAGATTGTTTCTATGAAGTAGAAGGTGTTTTTAATGTACCTAAAACAGTTTTCTATCCTCAACCAAGAGTTGAAT
CCCAAGTAGTTAAGTTAACTAAAAGGTCAGAAGCGAAAATTAATGATGACTATCAGGAAGATTTTATTAAATTTGTCAAT
CAGGCTTTTAATTCTAGGAGAAAAACCCTGGTGAATAATATTTTATCTATATTTACGGGAGAAAAAAGCGAATTGTCCCA
AATACTTGAAAACAATGGATTTTCAGCCGGGATAAGGGGAGAGCAGTTAACTGTAGATGAATTTGCTCAAATTTTCAAAA
TCATTTATAATAGAATTAAATACTCTTAA

Upstream 100 bases:

>100_bases
TTTAGCGCCCCATGGTGTTGGCGATGTCAAAGTAAAAGTTCTAGAAAACGACAATTAATATAAGTGAGCGGTTATGCTAA
AACCAGGAGTGTGAGTTGAA

Downstream 100 bases:

>100_bases
GATCTTCCAGTGAATAATTTAAACTAGAAAGGTAGTGGTTTTTAGCTATGTACCGAAGCCCATCTAGGGGTGAGTTAAAC
TTAACAGAAGTTTATTGGGA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MNLFSPKEVSQLLKRYEIHPKKSLGQNFLVDGNIIQKIIAAAELKEQDIVLEIGPGLGTLTRDMSFYVNEIFAIELDQRM
IDILQETVGSCDNVNIIHNDALKLDYQELISDFIEFSPAQLQCKSKQINPKNLKAVSNLPYYIASPLVLKLAKEKVPLSV
MVLMVQREVADRFTASPGSKNYGAVTVLLDCFYEVEGVFNVPKTVFYPQPRVESQVVKLTKRSEAKINDDYQEDFIKFVN
QAFNSRRKTLVNNILSIFTGEKSELSQILENNGFSAGIRGEQLTVDEFAQIFKIIYNRIKYS

Sequences:

>Translated_302_residues
MNLFSPKEVSQLLKRYEIHPKKSLGQNFLVDGNIIQKIIAAAELKEQDIVLEIGPGLGTLTRDMSFYVNEIFAIELDQRM
IDILQETVGSCDNVNIIHNDALKLDYQELISDFIEFSPAQLQCKSKQINPKNLKAVSNLPYYIASPLVLKLAKEKVPLSV
MVLMVQREVADRFTASPGSKNYGAVTVLLDCFYEVEGVFNVPKTVFYPQPRVESQVVKLTKRSEAKINDDYQEDFIKFVN
QAFNSRRKTLVNNILSIFTGEKSELSQILENNGFSAGIRGEQLTVDEFAQIFKIIYNRIKYS
>Mature_302_residues
MNLFSPKEVSQLLKRYEIHPKKSLGQNFLVDGNIIQKIIAAAELKEQDIVLEIGPGLGTLTRDMSFYVNEIFAIELDQRM
IDILQETVGSCDNVNIIHNDALKLDYQELISDFIEFSPAQLQCKSKQINPKNLKAVSNLPYYIASPLVLKLAKEKVPLSV
MVLMVQREVADRFTASPGSKNYGAVTVLLDCFYEVEGVFNVPKTVFYPQPRVESQVVKLTKRSEAKINDDYQEDFIKFVN
QAFNSRRKTLVNNILSIFTGEKSELSQILENNGFSAGIRGEQLTVDEFAQIFKIIYNRIKYS

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=298, Percent_Identity=26.1744966442953, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI7657198, Length=254, Percent_Identity=31.8897637795276, Blast_Score=99, Evalue=7e-21,
Organism=Escherichia coli, GI1786236, Length=271, Percent_Identity=29.8892988929889, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI25141369, Length=294, Percent_Identity=29.2517006802721, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI25146882, Length=252, Percent_Identity=29.7619047619048, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324989, Length=196, Percent_Identity=31.6326530612245, Blast_Score=96, Evalue=7e-21,
Organism=Drosophila melanogaster, GI21358017, Length=198, Percent_Identity=34.3434343434343, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21357273, Length=312, Percent_Identity=25.6410256410256, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_NATTJ (B2A3L9)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001916233.1
- ProteinModelPortal:   B2A3L9
- SMR:   B2A3L9
- GeneID:   6314255
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_0046
- HOGENOM:   HBG319664
- OMA:   AATIGKN
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 34317; Mature: 34317

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 27-27 BINDING 29-29 BINDING 54-54 BINDING 75-75 BINDING 100-100 BINDING 138-138

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLFSPKEVSQLLKRYEIHPKKSLGQNFLVDGNIIQKIIAAAELKEQDIVLEIGPGLGTL
CCCCCHHHHHHHHHHHCCCCCHHCCCCEEECCHHHHHHHHHHHHCCCCEEEEECCCCCHH
TRDMSFYVNEIFAIELDQRMIDILQETVGSCDNVNIIHNDALKLDYQELISDFIEFSPAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHCCHHHHHHHHHHCCCHH
LQCKSKQINPKNLKAVSNLPYYIASPLVLKLAKEKVPLSVMVLMVQREVADRFTASPGSK
EEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCC
NYGAVTVLLDCFYEVEGVFNVPKTVFYPQPRVESQVVKLTKRSEAKINDDYQEDFIKFVN
CCCHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
QAFNSRRKTLVNNILSIFTGEKSELSQILENNGFSAGIRGEQLTVDEFAQIFKIIYNRIK
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHHHHC
YS
CC
>Mature Secondary Structure
MNLFSPKEVSQLLKRYEIHPKKSLGQNFLVDGNIIQKIIAAAELKEQDIVLEIGPGLGTL
CCCCCHHHHHHHHHHHCCCCCHHCCCCEEECCHHHHHHHHHHHHCCCCEEEEECCCCCHH
TRDMSFYVNEIFAIELDQRMIDILQETVGSCDNVNIIHNDALKLDYQELISDFIEFSPAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHCCHHHHHHHHHHCCCHH
LQCKSKQINPKNLKAVSNLPYYIASPLVLKLAKEKVPLSVMVLMVQREVADRFTASPGSK
EEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCC
NYGAVTVLLDCFYEVEGVFNVPKTVFYPQPRVESQVVKLTKRSEAKINDDYQEDFIKFVN
CCCHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
QAFNSRRKTLVNNILSIFTGEKSELSQILENNGFSAGIRGEQLTVDEFAQIFKIIYNRIK
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHHHHC
YS
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA