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The map label for this gene is tmk [H]

Identifier: 188584675

GI number: 188584675

Start: 48342

End: 48962

Strand: Direct

Name: tmk [H]

Synonym: Nther_0033

Alternate gene names: 188584675

Gene position: 48342-48962 (Clockwise)

Preceding gene: 188584674

Following gene: 188584676

Centisome position: 1.53

GC content: 35.43

Gene sequence:

>621_bases
TTGTCTAAGAGAGGAAAATTTATAACCTTTGAAGGCATAGATGGTTGTGGAAAAAGTACTCAATTAAAAAAAGTAACCTC
TAAATTAAAAAAACTAGGGTTTAATGTGGTTTCAACCCGTGAACCTGGTGGAACTAGTGTAGGGGATGCAATTAGAGAAA
TACTTTTAGCTCCGAAACATTATAATATGACTATTGAAACAGAAGCACTTTTATATGCGGCTTCACGTGTACAACATATT
GAAGAATTTATTATTCCCAACTTAGCTGAGGATAACATAGTATTATGTGATCGCTTTGTTGATTCCAGTGTGGCTTATCA
AGGGATAAAGCTCTCCCATGAAGAAGTTATCCGTGTAAATCAACCGGCTTATGATAAAGTGACTCCTGATATAACATTTT
TAATAGATATAACTCCCGAACGTGCTGCGAAACGGTTAGGTATAGAAAAAGACCGGATTGAAAATAGGGGGGTGAATTAT
CAATCTCAAGTCAGGGAAAATTACCTTTTTCTAGCTGAAGAAGAACCTGATAGAATAGTGATTATTAATGGCGACCAATC
CGAAGAGAAAGTTTTCGAAGAGTTATGGAATGTTTTGGAAGACATATTGATAAGAAACTGA

Upstream 100 bases:

>100_bases
TACGTAAAAATAAAGAAAAACAAGTAGAAAAAGTATTAAGACATATTTTTAAAAAAGAAGTTGAAGAAAATGATTCAGAA
ATGTAAAGGGGATGGGGAGA

Downstream 100 bases:

>100_bases
CTAAATTATTAACTTGAATGAATTACGGGGAGAGGAGGTTAAAAATGAAAATGAAGTTGATAATTGTTATTTTGCAGGAT
AAAGATACGCCCCCTTTATT

Product: dTMP kinase

Products: NA

Alternate protein names: dTMP kinase [H]

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MSKRGKFITFEGIDGCGKSTQLKKVTSKLKKLGFNVVSTREPGGTSVGDAIREILLAPKHYNMTIETEALLYAASRVQHI
EEFIIPNLAEDNIVLCDRFVDSSVAYQGIKLSHEEVIRVNQPAYDKVTPDITFLIDITPERAAKRLGIEKDRIENRGVNY
QSQVRENYLFLAEEEPDRIVIINGDQSEEKVFEELWNVLEDILIRN

Sequences:

>Translated_206_residues
MSKRGKFITFEGIDGCGKSTQLKKVTSKLKKLGFNVVSTREPGGTSVGDAIREILLAPKHYNMTIETEALLYAASRVQHI
EEFIIPNLAEDNIVLCDRFVDSSVAYQGIKLSHEEVIRVNQPAYDKVTPDITFLIDITPERAAKRLGIEKDRIENRGVNY
QSQVRENYLFLAEEEPDRIVIINGDQSEEKVFEELWNVLEDILIRN
>Mature_205_residues
SKRGKFITFEGIDGCGKSTQLKKVTSKLKKLGFNVVSTREPGGTSVGDAIREILLAPKHYNMTIETEALLYAASRVQHIE
EFIIPNLAEDNIVLCDRFVDSSVAYQGIKLSHEEVIRVNQPAYDKVTPDITFLIDITPERAAKRLGIEKDRIENRGVNYQ
SQVRENYLFLAEEEPDRIVIINGDQSEEKVFEELWNVLEDILIRN

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis [H]

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1787340, Length=200, Percent_Identity=34, Blast_Score=100, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17535647, Length=163, Percent_Identity=32.5153374233129, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322517, Length=214, Percent_Identity=28.5046728971963, Blast_Score=63, Evalue=4e-11,
Organism=Drosophila melanogaster, GI28573735, Length=202, Percent_Identity=27.2277227722772, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018094 [H]

Pfam domain/function: PF02223 Thymidylate_kin [H]

EC number: =2.7.4.9 [H]

Molecular weight: Translated: 23441; Mature: 23310

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRGKFITFEGIDGCGKSTQLKKVTSKLKKLGFNVVSTREPGGTSVGDAIREILLAPKH
CCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHCCCCC
YNMTIETEALLYAASRVQHIEEFIIPNLAEDNIVLCDRFVDSSVAYQGIKLSHEEVIRVN
CCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHCCHHHHCCEEECHHHHEECC
QPAYDKVTPDITFLIDITPERAAKRLGIEKDRIENRGVNYQSQVRENYLFLAEEEPDRIV
CCCCCCCCCCEEEEEEECHHHHHHHCCCCHHHHHHCCCCHHHHHHHCEEEEEECCCCEEE
IINGDQSEEKVFEELWNVLEDILIRN
EECCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKRGKFITFEGIDGCGKSTQLKKVTSKLKKLGFNVVSTREPGGTSVGDAIREILLAPKH
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHCCCCC
YNMTIETEALLYAASRVQHIEEFIIPNLAEDNIVLCDRFVDSSVAYQGIKLSHEEVIRVN
CCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHCCHHHHCCEEECHHHHEECC
QPAYDKVTPDITFLIDITPERAAKRLGIEKDRIENRGVNYQSQVRENYLFLAEEEPDRIV
CCCCCCCCCCEEEEEEECHHHHHHHCCCCHHHHHHCCCCHHHHHHHCEEEEEECCCCEEE
IINGDQSEEKVFEELWNVLEDILIRN
EECCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA