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The map label for this gene is ispG

Identifier: 188527525

GI number: 188527525

Start: 717845

End: 718924

Strand: Reverse

Name: ispG

Synonym: HPSH_03735

Alternate gene names: 188527525

Gene position: 718924-717845 (Counterclockwise)

Preceding gene: 188527530

Following gene: 188527524

Centisome position: 44.69

GC content: 42.5

Gene sequence:

>1080_bases
ATGCTAGAAAACAGAGTGAAGACCAAGCAAATTTTTATCGGTGGCGTGGCCATAGGAGGTGATGCTCCTATAAGCACGCA
AAGCATGACCTTTAGTAAAACCGCTGATATTGAAAGCACTAAAAATCAAATTGACAGACTCAAACTCGCCGGGGCTGATT
TAGTGAGGGTGGCGGTGAGTAATGAAAAGGACGCCCTAGCCTTAAAAGAATTGAAAAGAGTGTCCCCTTTGCCTTTAATC
GCTGATATTCATTTCCATTATAAATTCGCTCTCATTGCCGCTCAAAGCGTGGATGCGATCAGGATTAACCCCGGAAACAT
CGGCTCTAAAGACAAAATCAAAGCAGTAGTTGATGCTTGCAAAGAAAAAAACATCCCTATAAGAATTGGCGTGAATGCTG
GGAGTTTAGAAAAGCAGTTTGATCAAAAATACGGGCCCACCCCAAAAGGCATGGTAGAAAGCGCTTTGTATAACGCCAAA
CTTTTAGAAGATTTGGATTTTACTGATTTTAAGATTTCTTTAAAAGCGAGCGATGTGATACGCACCATAGAAGCTTATAG
GATGCTACGCCCTCTTGTGATCTATCCTTTCCATTTGGGGGTTACTGAAGCGGGTAATCTTTTTAGCTCCAGTATCAAAT
CCGCCATGGCTTTAGGGGGGCTTTTAATGGAGGGCATTGGGGATACGATGCGCGTATCCATCACAGGGGAATTAGAAAAT
GAAATCAAAGTGGCTAGAGCGATTTTACGCCATAGCGGGCGTTTGAAAGAAGGGATTAATTTGATTTCTTGCCCCACTTG
CGGGCGCATTGAAGCCAATTTAGTGGATATGGCGAGCAAGGTAGAAAAACGCCTAAGCCACATTAAAACCCCTTTAGACA
TTAGCGTGATGGGTTGCGTGGTGAATGCTTTAGGTGAAGCCAAGCATGCAGACATGGCGATCGCTTTTGGGAATCGCAGC
GGTTTGATCATTAAAGAGGGTAAAGTCATTCACAAACTGGCTGAAAAGGATTTGTTTGAAACTTTTGTGATAGAAGTGGA
AAATTTAGCTAAAGAAAGAGAAAAAAGTTTAAAGGATTAG

Upstream 100 bases:

>100_bases
AATGGAATAGTTAATCTTACCTAAAATTTAAGCGTTATGGTGTGAAACCACTAAGAAAGAGTATAATAGCGAGTGAGAAT
TTAACTGATGAAGAGGTTTA

Downstream 100 bases:

>100_bases
GCATGATCAATAAGTTTAAAAATTTTGTGAGCAACTACCAGCAATCTAACCACTATAGAGAGCCTTTAGGCTTTGGCATT
GCCAGAGTGGATATTGCCCC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MLENRVKTKQIFIGGVAIGGDAPISTQSMTFSKTADIESTKNQIDRLKLAGADLVRVAVSNEKDALALKELKRVSPLPLI
ADIHFHYKFALIAAQSVDAIRINPGNIGSKDKIKAVVDACKEKNIPIRIGVNAGSLEKQFDQKYGPTPKGMVESALYNAK
LLEDLDFTDFKISLKASDVIRTIEAYRMLRPLVIYPFHLGVTEAGNLFSSSIKSAMALGGLLMEGIGDTMRVSITGELEN
EIKVARAILRHSGRLKEGINLISCPTCGRIEANLVDMASKVEKRLSHIKTPLDISVMGCVVNALGEAKHADMAIAFGNRS
GLIIKEGKVIHKLAEKDLFETFVIEVENLAKEREKSLKD

Sequences:

>Translated_359_residues
MLENRVKTKQIFIGGVAIGGDAPISTQSMTFSKTADIESTKNQIDRLKLAGADLVRVAVSNEKDALALKELKRVSPLPLI
ADIHFHYKFALIAAQSVDAIRINPGNIGSKDKIKAVVDACKEKNIPIRIGVNAGSLEKQFDQKYGPTPKGMVESALYNAK
LLEDLDFTDFKISLKASDVIRTIEAYRMLRPLVIYPFHLGVTEAGNLFSSSIKSAMALGGLLMEGIGDTMRVSITGELEN
EIKVARAILRHSGRLKEGINLISCPTCGRIEANLVDMASKVEKRLSHIKTPLDISVMGCVVNALGEAKHADMAIAFGNRS
GLIIKEGKVIHKLAEKDLFETFVIEVENLAKEREKSLKD
>Mature_359_residues
MLENRVKTKQIFIGGVAIGGDAPISTQSMTFSKTADIESTKNQIDRLKLAGADLVRVAVSNEKDALALKELKRVSPLPLI
ADIHFHYKFALIAAQSVDAIRINPGNIGSKDKIKAVVDACKEKNIPIRIGVNAGSLEKQFDQKYGPTPKGMVESALYNAK
LLEDLDFTDFKISLKASDVIRTIEAYRMLRPLVIYPFHLGVTEAGNLFSSSIKSAMALGGLLMEGIGDTMRVSITGELEN
EIKVARAILRHSGRLKEGINLISCPTCGRIEANLVDMASKVEKRLSHIKTPLDISVMGCVVNALGEAKHADMAIAFGNRS
GLIIKEGKVIHKLAEKDLFETFVIEVENLAKEREKSLKD

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=341, Percent_Identity=46.9208211143695, Blast_Score=315, Evalue=2e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 39195; Mature: 39195

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLENRVKTKQIFIGGVAIGGDAPISTQSMTFSKTADIESTKNQIDRLKLAGADLVRVAVS
CCCCCCCEEEEEEEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEC
NEKDALALKELKRVSPLPLIADIHFHYKFALIAAQSVDAIRINPGNIGSKDKIKAVVDAC
CCCHHHHHHHHHHCCCCCEEEEEEEEEEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHH
KEKNIPIRIGVNAGSLEKQFDQKYGPTPKGMVESALYNAKLLEDLDFTDFKISLKASDVI
HCCCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHH
RTIEAYRMLRPLVIYPFHLGVTEAGNLFSSSIKSAMALGGLLMEGIGDTMRVSITGELEN
HHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHH
EIKVARAILRHSGRLKEGINLISCPTCGRIEANLVDMASKVEKRLSHIKTPLDISVMGCV
HHHHHHHHHHHCCCHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VNALGEAKHADMAIAFGNRSGLIIKEGKVIHKLAEKDLFETFVIEVENLAKEREKSLKD
HHHHCCCCCCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLENRVKTKQIFIGGVAIGGDAPISTQSMTFSKTADIESTKNQIDRLKLAGADLVRVAVS
CCCCCCCEEEEEEEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEC
NEKDALALKELKRVSPLPLIADIHFHYKFALIAAQSVDAIRINPGNIGSKDKIKAVVDAC
CCCHHHHHHHHHHCCCCCEEEEEEEEEEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHH
KEKNIPIRIGVNAGSLEKQFDQKYGPTPKGMVESALYNAKLLEDLDFTDFKISLKASDVI
HCCCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHH
RTIEAYRMLRPLVIYPFHLGVTEAGNLFSSSIKSAMALGGLLMEGIGDTMRVSITGELEN
HHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHH
EIKVARAILRHSGRLKEGINLISCPTCGRIEANLVDMASKVEKRLSHIKTPLDISVMGCV
HHHHHHHHHHHCCCHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VNALGEAKHADMAIAFGNRSGLIIKEGKVIHKLAEKDLFETFVIEVENLAKEREKSLKD
HHHHCCCCCCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA