Click here to switch to the map view.

The map label for this gene is cfrBIM [H]

Identifier: 188527466

GI number: 188527466

Start: 652186

End: 653400

Strand: Reverse

Name: cfrBIM [H]

Synonym: HPSH_03420

Alternate gene names: 188527466

Gene position: 653400-652186 (Counterclockwise)

Preceding gene: 188527467

Following gene: 188527465

Centisome position: 40.62

GC content: 30.04

Gene sequence:

>1215_bases
ATGTCTACAATATCAAAAAATAAACCATATAGTTTTATTAAAAACCTTGAGTTTTACACAATTAAACGCATTAAGGATAT
GGGTTCTCACCCTAGCGAAGACCATTTAAATAAATTGCTAGAATTGTTTAAACAAGATTTAAGTATTGATCTAAAAAGAG
AGATAGCAAGCTCTATTGGTAGGCAACTTGATGATGATATTATTTATAATTTTTTAAAGCAAGAAGCTTTTAAAGAACAT
TACATGGAAGTTGTTTATCAATTTTTACGCACTGCTTTATATAAATCTAAGGATATGCGATTCGCAAAATTATGTGATGA
CTTGTTGCAATACTATCAAAATGAAAACATGCAAAAAATGAAACAATACTATGACTATCGCCACACCAAAAAACCACCTT
TAAAAATTATAGAAAATATTATTAAAAAACCTTCTTTATTAATTGGGGATAATGCACAAACGCTCAATAAAATCGTTCCT
AATTCTATCAATCTTATTTTTACTTCACCACCTTACTATAATGCTAGAATTTATAGTGATTATAAAAATTACAAAGATTA
TCTAAATGCCATGTCTCAAAGTTTAAAGGCTTGTTTTAGAGTGTTAGAAGAGGGGCGTTTTATTATCATTAATGTTTCAC
CTGTGATCACCAAGAGAGCTGGGCGTGAGTTTGAAAGTGTGCGTTATCCTATTCATTTTGATTTCCATCAAATTTTAATT
GACAATGGATTTTACTTTGTAGATGAAATCCTATGGATTAAACCTGATTTTAGTGTGCCTAATCGTATTGGAGGATATTT
ACAAAATAAAAAACCTTTAGGATACAAGCCTAATTGTGTGAGTGAAAGTTTGTTAGTTTATCGTAAAAAAGCCCCTTTTC
TACTAGATAAAAATATTAAAATAGCTGAAAAACGATTAAAACCAAGCAAACAAAATAATACTTTATTTGGAAAAAAAGAA
TTGCCTATAGAAACTACCAATTGTTGGTATATTACTCCAAAGTCTAGTAAAGACCATCCGGCTATATTCCCTGAAAGTCT
TTGTGAAAGAGTGCTAAATTACTACTCTTTTGAAAATGAAGTTGTATGTGACCCTTTTGCCGGTAGTGGCACTTTTGGAA
TGGTTGCAAAATCTATGGGGCGTATTCCTTTATTATGTGAACAACATCCAAAATATGCTCAAAATCTAATCAAACTTGGT
TTTAAGGAAATCTAA

Upstream 100 bases:

>100_bases
CGTTTTTATTAACGCTTTAGAGAAAGAAATTAGGGAGTTTGCTTGAACTTCTATGATTATATAAACAATCTATCTAATAT
TTTTAATAAAAGAGTCTTTT

Downstream 100 bases:

>100_bases
AAATGAATTTTAATGAATTAGCTTTAAATCATACGATTGATTTGCTCTTAAAAGGAAAAGATTATAGAGAAGTAGTTCTA
AACACTATTAACACAGAGTT

Product: putative type II cytosine specific methyltransferase

Products: NA

Alternate protein names: M.CfrBI; N(4)- cytosine-specific methyltransferase CfrBI [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MSTISKNKPYSFIKNLEFYTIKRIKDMGSHPSEDHLNKLLELFKQDLSIDLKREIASSIGRQLDDDIIYNFLKQEAFKEH
YMEVVYQFLRTALYKSKDMRFAKLCDDLLQYYQNENMQKMKQYYDYRHTKKPPLKIIENIIKKPSLLIGDNAQTLNKIVP
NSINLIFTSPPYYNARIYSDYKNYKDYLNAMSQSLKACFRVLEEGRFIIINVSPVITKRAGREFESVRYPIHFDFHQILI
DNGFYFVDEILWIKPDFSVPNRIGGYLQNKKPLGYKPNCVSESLLVYRKKAPFLLDKNIKIAEKRLKPSKQNNTLFGKKE
LPIETTNCWYITPKSSKDHPAIFPESLCERVLNYYSFENEVVCDPFAGSGTFGMVAKSMGRIPLLCEQHPKYAQNLIKLG
FKEI

Sequences:

>Translated_404_residues
MSTISKNKPYSFIKNLEFYTIKRIKDMGSHPSEDHLNKLLELFKQDLSIDLKREIASSIGRQLDDDIIYNFLKQEAFKEH
YMEVVYQFLRTALYKSKDMRFAKLCDDLLQYYQNENMQKMKQYYDYRHTKKPPLKIIENIIKKPSLLIGDNAQTLNKIVP
NSINLIFTSPPYYNARIYSDYKNYKDYLNAMSQSLKACFRVLEEGRFIIINVSPVITKRAGREFESVRYPIHFDFHQILI
DNGFYFVDEILWIKPDFSVPNRIGGYLQNKKPLGYKPNCVSESLLVYRKKAPFLLDKNIKIAEKRLKPSKQNNTLFGKKE
LPIETTNCWYITPKSSKDHPAIFPESLCERVLNYYSFENEVVCDPFAGSGTFGMVAKSMGRIPLLCEQHPKYAQNLIKLG
FKEI
>Mature_403_residues
STISKNKPYSFIKNLEFYTIKRIKDMGSHPSEDHLNKLLELFKQDLSIDLKREIASSIGRQLDDDIIYNFLKQEAFKEHY
MEVVYQFLRTALYKSKDMRFAKLCDDLLQYYQNENMQKMKQYYDYRHTKKPPLKIIENIIKKPSLLIGDNAQTLNKIVPN
SINLIFTSPPYYNARIYSDYKNYKDYLNAMSQSLKACFRVLEEGRFIIINVSPVITKRAGREFESVRYPIHFDFHQILID
NGFYFVDEILWIKPDFSVPNRIGGYLQNKKPLGYKPNCVSESLLVYRKKAPFLLDKNIKIAEKRLKPSKQNNTLFGKKEL
PIETTNCWYITPKSSKDHPAIFPESLCERVLNYYSFENEVVCDPFAGSGTFGMVAKSMGRIPLLCEQHPKYAQNLIKLGF
KEI

Specific function: This methylase recognizes the double-stranded sequence CCWWGG, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the CfrBI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 47412; Mature: 47280

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTISKNKPYSFIKNLEFYTIKRIKDMGSHPSEDHLNKLLELFKQDLSIDLKREIASSIG
CCCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RQLDDDIIYNFLKQEAFKEHYMEVVYQFLRTALYKSKDMRFAKLCDDLLQYYQNENMQKM
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHH
KQYYDYRHTKKPPLKIIENIIKKPSLLIGDNAQTLNKIVPNSINLIFTSPPYYNARIYSD
HHHHHHHCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHCCCCCEEEECCCCCCCCEEHHH
YKNYKDYLNAMSQSLKACFRVLEEGRFIIINVSPVITKRAGREFESVRYPIHFDFHQILI
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCCHHHCCCCEEECHHHHHH
DNGFYFVDEILWIKPDFSVPNRIGGYLQNKKPLGYKPNCVSESLLVYRKKAPFLLDKNIK
HCCCEEEHHHEEECCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCH
IAEKRLKPSKQNNTLFGKKELPIETTNCWYITPKSSKDHPAIFPESLCERVLNYYSFENE
HHHHHCCCCCCCCCCCCCCCCCEECCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCC
VVCDPFAGSGTFGMVAKSMGRIPLLCEQHPKYAQNLIKLGFKEI
EEECCCCCCCHHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STISKNKPYSFIKNLEFYTIKRIKDMGSHPSEDHLNKLLELFKQDLSIDLKREIASSIG
CCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RQLDDDIIYNFLKQEAFKEHYMEVVYQFLRTALYKSKDMRFAKLCDDLLQYYQNENMQKM
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHH
KQYYDYRHTKKPPLKIIENIIKKPSLLIGDNAQTLNKIVPNSINLIFTSPPYYNARIYSD
HHHHHHHCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHCCCCCEEEECCCCCCCCEEHHH
YKNYKDYLNAMSQSLKACFRVLEEGRFIIINVSPVITKRAGREFESVRYPIHFDFHQILI
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCCHHHCCCCEEECHHHHHH
DNGFYFVDEILWIKPDFSVPNRIGGYLQNKKPLGYKPNCVSESLLVYRKKAPFLLDKNIK
HCCCEEEHHHEEECCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCH
IAEKRLKPSKQNNTLFGKKELPIETTNCWYITPKSSKDHPAIFPESLCERVLNYYSFENE
HHHHHCCCCCCCCCCCCCCCCCEECCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCC
VVCDPFAGSGTFGMVAKSMGRIPLLCEQHPKYAQNLIKLGFKEI
EEECCCCCCCHHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8335262 [H]