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The map label for this gene is dut

Identifier: 188527290

GI number: 188527290

Start: 479751

End: 480188

Strand: Direct

Name: dut

Synonym: HPSH_02485

Alternate gene names: 188527290

Gene position: 479751-480188 (Clockwise)

Preceding gene: 188527289

Following gene: 188527291

Centisome position: 29.83

GC content: 41.78

Gene sequence:

>438_bases
ATGAAAATTAAAATCCAAAAAATCCACCCAAACGCCCTTATCCCTAAATACCAAACCGAGGGTTCTTCAGGCTTTGATTT
GCATGCTATAGAAGAAGTAATGATCAAACCTCATAGCGTGGGCTTGGTGAAAATAGGGATTTGTTTGTCTTTAGAAGTGG
GGTATGAATTGCAAGTGCGCACCCGTAGCGGTTTGGCTTTGAATCATCAGGTGGTAGTGTTAAATTCTCCTGGCACGGTG
GATAATGATTATAGGGGCGAAATTAAGGTCATTTTAGCGAATTTGAGCGATAAAGATTTTAAGGTTCAAGTAGGGGATAG
GATCGCTCAAGGGGTGGTTCAAAAAACTTATAAAGCCGAATTTATAGAATGCGAACAATTAGATGAAACTTCAAGGGGTA
GCGGGGGCTTTGGCAGCACAGGAGTGAGTAAGGCATGA

Upstream 100 bases:

>100_bases
TTGATAGGTAAGAGCAAGGGCGATGCGGTGAGCATTCAATTGCCTAATGGTGAGAGCGATTTTGAAATTTTAGACATTTA
TTATAAAGAGATTTGTTTTG

Downstream 100 bases:

>100_bases
GTATTAAGGAAAATTTAGAGCAAGTTAAAAACGAATTTAAAAGCGATGAAAAGCTTTTAGAAGGGGCGTTTAGATTAGAA
AAGTTTTTCAAACGCTACAA

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase [H]

Number of amino acids: Translated: 145; Mature: 145

Protein sequence:

>145_residues
MKIKIQKIHPNALIPKYQTEGSSGFDLHAIEEVMIKPHSVGLVKIGICLSLEVGYELQVRTRSGLALNHQVVVLNSPGTV
DNDYRGEIKVILANLSDKDFKVQVGDRIAQGVVQKTYKAEFIECEQLDETSRGSGGFGSTGVSKA

Sequences:

>Translated_145_residues
MKIKIQKIHPNALIPKYQTEGSSGFDLHAIEEVMIKPHSVGLVKIGICLSLEVGYELQVRTRSGLALNHQVVVLNSPGTV
DNDYRGEIKVILANLSDKDFKVQVGDRIAQGVVQKTYKAEFIECEQLDETSRGSGGFGSTGVSKA
>Mature_145_residues
MKIKIQKIHPNALIPKYQTEGSSGFDLHAIEEVMIKPHSVGLVKIGICLSLEVGYELQVRTRSGLALNHQVVVLNSPGTV
DNDYRGEIKVILANLSDKDFKVQVGDRIAQGVVQKTYKAEFIECEQLDETSRGSGGFGSTGVSKA

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA [H]

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family [H]

Homologues:

Organism=Homo sapiens, GI70906444, Length=141, Percent_Identity=37.5886524822695, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI4503423, Length=141, Percent_Identity=37.5886524822695, Blast_Score=101, Evalue=2e-22,
Organism=Homo sapiens, GI70906441, Length=141, Percent_Identity=37.5886524822695, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1790071, Length=131, Percent_Identity=35.8778625954198, Blast_Score=86, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71988561, Length=141, Percent_Identity=39.7163120567376, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319729, Length=140, Percent_Identity=36.4285714285714, Blast_Score=99, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19921126, Length=140, Percent_Identity=36.4285714285714, Blast_Score=93, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24583610, Length=140, Percent_Identity=36.4285714285714, Blast_Score=93, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008180
- InterPro:   IPR008181 [H]

Pfam domain/function: PF00692 dUTPase [H]

EC number: =3.6.1.23 [H]

Molecular weight: Translated: 15797; Mature: 15797

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKIQKIHPNALIPKYQTEGSSGFDLHAIEEVMIKPHSVGLVKIGICLSLEVGYELQVR
CEEEEEEECCCCCCCCEECCCCCCCCHHHHHHHHCCCCCCCEEEEEEEEEEECCCEEEEE
TRSGLALNHQVVVLNSPGTVDNDYRGEIKVILANLSDKDFKVQVGDRIAQGVVQKTYKAE
ECCCEEECCEEEEECCCCCCCCCCCCEEEEEEECCCCCCEEEEECHHHHHHHHHHHHHCC
FIECEQLDETSRGSGGFGSTGVSKA
EEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIKIQKIHPNALIPKYQTEGSSGFDLHAIEEVMIKPHSVGLVKIGICLSLEVGYELQVR
CEEEEEEECCCCCCCCEECCCCCCCCHHHHHHHHCCCCCCCEEEEEEEEEEECCCEEEEE
TRSGLALNHQVVVLNSPGTVDNDYRGEIKVILANLSDKDFKVQVGDRIAQGVVQKTYKAE
ECCCEEECCEEEEECCCCCCCCCCCCEEEEEEECCCCCCEEEEECHHHHHHHHHHHHHCC
FIECEQLDETSRGSGGFGSTGVSKA
EEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA