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The map label for this gene is bsp6IM [H]

Identifier: 188527161

GI number: 188527161

Start: 348903

End: 349832

Strand: Direct

Name: bsp6IM [H]

Synonym: HPSH_01840

Alternate gene names: 188527161

Gene position: 348903-349832 (Clockwise)

Preceding gene: 188527160

Following gene: 188527162

Centisome position: 21.69

GC content: 33.55

Gene sequence:

>930_bases
ATGAAAGTAGGCTCACTTTTTGCTGGGATTGGAGGGTTTGAATGCGCGTTTTTGCAAGCGGGCTTTGAAATTGATTGGGC
GAATGAATTGGACAAAGACGCATGCAACACTTATAGGGCTAATTTCAAACACAAGCTTTTAGAACAAGACATTAAAGATT
TAAACCCTAATGAATTAGAAGATGTTGGGTTGATAAGCGCAGGGTTTCCTTGTCAAGCGTTCAGCATTGCCGGGTTGCAA
AAAGGACTTAATGATGAAAGAGGAGCGATTGTTATGGAAATGTTTCGCATCATTCAAGCTAAAAAACCTCAAGTTTTATT
ATTAGAAAATGTAAAAAATTTAGTGAGTCATAATAAGGGGGAAACCTTAAAGTTTATTTTAGAAACTTTAAAAAACTTAG
GCTATTTTGTCCATTATAAAATACTAAACACTTATGAATATTCTACTATACCACAAAATAGAGAGCGGATTTATATTATA
GGATTTTTAGATAAAAACCATTACAAGCGCTTTCATTTTCCTTTAAAAATCAATCACACACAAAGTATTAAGGATTTATT
AGAAATAAAAGTGGGTGAGGAATTTTATTACAAGCAATATCGTTTTTATGAGACTTTAAAAAAAGAAATCACCAAAAGAG
ACACTTGCTACCAATGGCGTAGGCATTATGTAAGAGAAAATAAAAACAATCTTTGCCCCACTCTTACAGCCAACATGGGG
ACAGGAGGGCATAATGTGCCTTTAGTTTTAGATGAGCAAGGCATAAGAAAACTCACGCCTAAAGAATGTCTGAATTTTCA
AGGTTTCGCTAAAGATTATGTTTTACCTAATACCGCTAAAAGCAAACTTTATAAACAAATTGGTAATTCTGTGAGTGTGC
CTGTGATTAAAGCTTTAGCGTTAGAAATTAAAAAGGTTGTTTATGGATAA

Upstream 100 bases:

>100_bases
AAAAGCAAAAAGAAGGCAAGAAACGCATGAAAGCTATCGGCAAGGTGGAGCTTCCTCAAGAAGCGTTTTTAGCGATATTA
AAGATTGATTAGGGTGTTTG

Downstream 100 bases:

>100_bases
TTATACGCATCAAACTTGGGTCATCAATTTAACCGGTATTGACAAGGGGATTTTTCGCACAATGAATGGCAACGCTGATG
AACTCATTGCTATAGGAAGG

Product: DNA-cytosine methyltransferase

Products: NA

Alternate protein names: M.Bsp6I; Cytosine-specific methyltransferase Bsp6I [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKVGSLFAGIGGFECAFLQAGFEIDWANELDKDACNTYRANFKHKLLEQDIKDLNPNELEDVGLISAGFPCQAFSIAGLQ
KGLNDERGAIVMEMFRIIQAKKPQVLLLENVKNLVSHNKGETLKFILETLKNLGYFVHYKILNTYEYSTIPQNRERIYII
GFLDKNHYKRFHFPLKINHTQSIKDLLEIKVGEEFYYKQYRFYETLKKEITKRDTCYQWRRHYVRENKNNLCPTLTANMG
TGGHNVPLVLDEQGIRKLTPKECLNFQGFAKDYVLPNTAKSKLYKQIGNSVSVPVIKALALEIKKVVYG

Sequences:

>Translated_309_residues
MKVGSLFAGIGGFECAFLQAGFEIDWANELDKDACNTYRANFKHKLLEQDIKDLNPNELEDVGLISAGFPCQAFSIAGLQ
KGLNDERGAIVMEMFRIIQAKKPQVLLLENVKNLVSHNKGETLKFILETLKNLGYFVHYKILNTYEYSTIPQNRERIYII
GFLDKNHYKRFHFPLKINHTQSIKDLLEIKVGEEFYYKQYRFYETLKKEITKRDTCYQWRRHYVRENKNNLCPTLTANMG
TGGHNVPLVLDEQGIRKLTPKECLNFQGFAKDYVLPNTAKSKLYKQIGNSVSVPVIKALALEIKKVVYG
>Mature_309_residues
MKVGSLFAGIGGFECAFLQAGFEIDWANELDKDACNTYRANFKHKLLEQDIKDLNPNELEDVGLISAGFPCQAFSIAGLQ
KGLNDERGAIVMEMFRIIQAKKPQVLLLENVKNLVSHNKGETLKFILETLKNLGYFVHYKILNTYEYSTIPQNRERIYII
GFLDKNHYKRFHFPLKINHTQSIKDLLEIKVGEEFYYKQYRFYETLKKEITKRDTCYQWRRHYVRENKNNLCPTLTANMG
TGGHNVPLVLDEQGIRKLTPKECLNFQGFAKDYVLPNTAKSKLYKQIGNSVSVPVIKALALEIKKVVYG

Specific function: This methylase recognizes the double-stranded sequence GCNGC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the Bsp6I endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=355, Percent_Identity=29.0140845070423, Blast_Score=114, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 35646; Mature: 35646

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGSLFAGIGGFECAFLQAGFEIDWANELDKDACNTYRANFKHKLLEQDIKDLNPNELE
CCCCHHHHCCCCHHEEEHHCCCEEECHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
DVGLISAGFPCQAFSIAGLQKGLNDERGAIVMEMFRIIQAKKPQVLLLENVKNLVSHNKG
HCCEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCC
ETLKFILETLKNLGYFVHYKILNTYEYSTIPQNRERIYIIGFLDKNHYKRFHFPLKINHT
CHHHHHHHHHHHCCEEEEEEEECCEECCCCCCCCCEEEEEEEECCCCCEEEECCEEECCH
QSIKDLLEIKVGEEFYYKQYRFYETLKKEITKRDTCYQWRRHYVRENKNNLCPTLTANMG
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEECCC
TGGHNVPLVLDEQGIRKLTPKECLNFQGFAKDYVLPNTAKSKLYKQIGNSVSVPVIKALA
CCCCCCCEEECCCCCCCCCHHHHCCCCCCHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHH
LEIKKVVYG
HHHHHHHCC
>Mature Secondary Structure
MKVGSLFAGIGGFECAFLQAGFEIDWANELDKDACNTYRANFKHKLLEQDIKDLNPNELE
CCCCHHHHCCCCHHEEEHHCCCEEECHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
DVGLISAGFPCQAFSIAGLQKGLNDERGAIVMEMFRIIQAKKPQVLLLENVKNLVSHNKG
HCCEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCC
ETLKFILETLKNLGYFVHYKILNTYEYSTIPQNRERIYIIGFLDKNHYKRFHFPLKINHT
CHHHHHHHHHHHCCEEEEEEEECCEECCCCCCCCCEEEEEEEECCCCCEEEECCEEECCH
QSIKDLLEIKVGEEFYYKQYRFYETLKKEITKRDTCYQWRRHYVRENKNNLCPTLTANMG
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEECCC
TGGHNVPLVLDEQGIRKLTPKECLNFQGFAKDYVLPNTAKSKLYKQIGNSVSVPVIKALA
CCCCCCCEEECCCCCCCCCHHHHCCCCCCHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHH
LEIKKVVYG
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7607501 [H]