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The map label for this gene is miaB [H]

Identifier: 188527075

GI number: 188527075

Start: 263630

End: 264910

Strand: Direct

Name: miaB [H]

Synonym: HPSH_01400

Alternate gene names: 188527075

Gene position: 263630-264910 (Clockwise)

Preceding gene: 188527074

Following gene: 188527076

Centisome position: 16.39

GC content: 43.09

Gene sequence:

>1281_bases
ATGAACTCTAGGGATAGCGAACATTTATTGAGCGAGCTCTCCAAACTAGACTATAAAGAAACTAGCGACCCTAAAGCAGC
GGATTTGATTTTAATCAACACTTGCAGCGTGCGCGAAAAACCCGAACGGAAATTGTTTTCAGAAATCGGTCAATTCGCTA
AAATCAAAAAACCCAACGCCAAAATCGGGGTTTGCGGTTGCACCGCAAGCCACATGGGAGCGGATATTTTGAAAAAAGCC
CCGAGCGTGAGCTTTGTGTTAGGGGCTAGGAATGTGTCTAAAATCTCTCAAGTGATCCATAAAGAAAAAGCGGTTGAAGT
GGCGATTGATTATGATGAAAGCGCGTATGCGTTTGAATTTTTTGAAAAAAAAGCTCAAATCAGATCGTTACTAAATATCT
CTATAGGTTGCGATAAGAAATGCGCTTATTGCATTGTCCCGCACACTAGGGGGAAAGAAATTTCTATCCCTATGGATTTG
ATTTTAAAAGAAGCTGAGAAATTGGCGAGTAACGGCACTAAAGAGCTGATGCTTTTGGGGCAGAATGTGAATAATTACGG
CGCGCGTTTCAGTAGCGAGCATGCGAAAGTGGATTTTAGCGATTTGTTGGATAAATTGAGCGAAATCCAAGGCATTGAAA
GGATACGATTCACTTCGCCTCACCCCTTGCACATGAATGATGCGTTTTTAGAGCGCTTTGCCAAAAACCCTAAAGTGTGC
AAGAGTATCCACATGCCCTTACAGAGCGGATCTAGCACGGTGTTAAAGATGATGCGAAGGGGTTATAGTAAGGAGTGGTT
TTTAAATAGGGTGGAGAAGTTAAAAGCGTTAGTGCCTGAAGTGGGCATTAGCACGGACATTATCGTAGGCTTCCCTAATG
AAAGCGATAAGGATTTTGAAGACACGATGGAAGTGCTAGAAAAAGTGCGCTTTGACACGCTCTATAGTTTCATTTATTCC
CCACGCCCTTTCACTGAAGCGGGAGCTTGGAAAGAAAGAGTGCCATTAGAGGTTTCATCTTCAAGATTGGAGAGGTTGCA
AAACAGGCACAAAGAAATTTTAGAAGAAAAAGCCAAGCTAGAAGTGGGTAAAACGCATGTGGTGTTGGTGGAAAATAGGC
GTGAAATTGACAATCAAATCGTGGGTTTTGAAGGGCGCAGCGATACGGGGAAATTCATTGAAGTAGCGTGTAAAGAAAAA
AGAAATCCGGGCGAGCTTGTAAGAGTGGAGATTGTTTCTCATTCCAAAGGGCGCTTGATAGCGACCGCTAAAGGCGACTA
A

Upstream 100 bases:

>100_bases
TGAGGTGAGGGTCAGCAACGATGAAAAAGATTTTCTTTATGAATTGCACATTATTTAAAGGCTTGTATTGAAAGTTTATA
TTGAAACAATGGGTTGTGCT

Downstream 100 bases:

>100_bases
TAAAAATAACCAATGAAAAAGCGGGTTTAAAGGCGAGAATTGAGTTTAAAATTTTTCAGGAAAAATATCGTTTTAAAGGT
TGTTCCACGCTTGGCGTTTG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MNSRDSEHLLSELSKLDYKETSDPKAADLILINTCSVREKPERKLFSEIGQFAKIKKPNAKIGVCGCTASHMGADILKKA
PSVSFVLGARNVSKISQVIHKEKAVEVAIDYDESAYAFEFFEKKAQIRSLLNISIGCDKKCAYCIVPHTRGKEISIPMDL
ILKEAEKLASNGTKELMLLGQNVNNYGARFSSEHAKVDFSDLLDKLSEIQGIERIRFTSPHPLHMNDAFLERFAKNPKVC
KSIHMPLQSGSSTVLKMMRRGYSKEWFLNRVEKLKALVPEVGISTDIIVGFPNESDKDFEDTMEVLEKVRFDTLYSFIYS
PRPFTEAGAWKERVPLEVSSSRLERLQNRHKEILEEKAKLEVGKTHVVLVENRREIDNQIVGFEGRSDTGKFIEVACKEK
RNPGELVRVEIVSHSKGRLIATAKGD

Sequences:

>Translated_426_residues
MNSRDSEHLLSELSKLDYKETSDPKAADLILINTCSVREKPERKLFSEIGQFAKIKKPNAKIGVCGCTASHMGADILKKA
PSVSFVLGARNVSKISQVIHKEKAVEVAIDYDESAYAFEFFEKKAQIRSLLNISIGCDKKCAYCIVPHTRGKEISIPMDL
ILKEAEKLASNGTKELMLLGQNVNNYGARFSSEHAKVDFSDLLDKLSEIQGIERIRFTSPHPLHMNDAFLERFAKNPKVC
KSIHMPLQSGSSTVLKMMRRGYSKEWFLNRVEKLKALVPEVGISTDIIVGFPNESDKDFEDTMEVLEKVRFDTLYSFIYS
PRPFTEAGAWKERVPLEVSSSRLERLQNRHKEILEEKAKLEVGKTHVVLVENRREIDNQIVGFEGRSDTGKFIEVACKEK
RNPGELVRVEIVSHSKGRLIATAKGD
>Mature_426_residues
MNSRDSEHLLSELSKLDYKETSDPKAADLILINTCSVREKPERKLFSEIGQFAKIKKPNAKIGVCGCTASHMGADILKKA
PSVSFVLGARNVSKISQVIHKEKAVEVAIDYDESAYAFEFFEKKAQIRSLLNISIGCDKKCAYCIVPHTRGKEISIPMDL
ILKEAEKLASNGTKELMLLGQNVNNYGARFSSEHAKVDFSDLLDKLSEIQGIERIRFTSPHPLHMNDAFLERFAKNPKVC
KSIHMPLQSGSSTVLKMMRRGYSKEWFLNRVEKLKALVPEVGISTDIIVGFPNESDKDFEDTMEVLEKVRFDTLYSFIYS
PRPFTEAGAWKERVPLEVSSSRLERLQNRHKEILEEKAKLEVGKTHVVLVENRREIDNQIVGFEGRSDTGKFIEVACKEK
RNPGELVRVEIVSHSKGRLIATAKGD

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=445, Percent_Identity=34.1573033707865, Blast_Score=232, Evalue=5e-61,
Organism=Homo sapiens, GI28872782, Length=445, Percent_Identity=34.1573033707865, Blast_Score=231, Evalue=7e-61,
Organism=Homo sapiens, GI93277076, Length=357, Percent_Identity=28.2913165266106, Blast_Score=144, Evalue=1e-34,
Organism=Escherichia coli, GI1786882, Length=415, Percent_Identity=36.8674698795181, Blast_Score=261, Evalue=7e-71,
Organism=Escherichia coli, GI1787057, Length=436, Percent_Identity=26.8348623853211, Blast_Score=162, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17553146, Length=460, Percent_Identity=28.695652173913, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI71996771, Length=257, Percent_Identity=30.7392996108949, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21356207, Length=406, Percent_Identity=31.2807881773399, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI19922432, Length=443, Percent_Identity=26.6365688487585, Blast_Score=141, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 48077; Mature: 48077

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSRDSEHLLSELSKLDYKETSDPKAADLILINTCSVREKPERKLFSEIGQFAKIKKPNA
CCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCC
KIGVCGCTASHMGADILKKAPSVSFVLGARNVSKISQVIHKEKAVEVAIDYDESAYAFEF
CEEEEECCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHEEEEEECCCCHHHHHH
FEKKAQIRSLLNISIGCDKKCAYCIVPHTRGKEISIPMDLILKEAEKLASNGTKELMLLG
HHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEECCCHHHHHHHHHHHHCCCCEEEEEEC
QNVNNYGARFSSEHAKVDFSDLLDKLSEIQGIERIRFTSPHPLHMNDAFLERFAKNPKVC
CCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHCCHHHH
KSIHMPLQSGSSTVLKMMRRGYSKEWFLNRVEKLKALVPEVGISTDIIVGFPNESDKDFE
HHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHH
DTMEVLEKVRFDTLYSFIYSPRPFTEAGAWKERVPLEVSSSRLERLQNRHKEILEEKAKL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCEECHHHHHHHHHHHHHHHHHHHHH
EVGKTHVVLVENRREIDNQIVGFEGRSDTGKFIEVACKEKRNPGELVRVEIVSHSKGRLI
CCCCEEEEEECCCHHHCCCEECCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCCEE
ATAKGD
EEECCC
>Mature Secondary Structure
MNSRDSEHLLSELSKLDYKETSDPKAADLILINTCSVREKPERKLFSEIGQFAKIKKPNA
CCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCC
KIGVCGCTASHMGADILKKAPSVSFVLGARNVSKISQVIHKEKAVEVAIDYDESAYAFEF
CEEEEECCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHEEEEEECCCCHHHHHH
FEKKAQIRSLLNISIGCDKKCAYCIVPHTRGKEISIPMDLILKEAEKLASNGTKELMLLG
HHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEECCCHHHHHHHHHHHHCCCCEEEEEEC
QNVNNYGARFSSEHAKVDFSDLLDKLSEIQGIERIRFTSPHPLHMNDAFLERFAKNPKVC
CCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHCCHHHH
KSIHMPLQSGSSTVLKMMRRGYSKEWFLNRVEKLKALVPEVGISTDIIVGFPNESDKDFE
HHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHH
DTMEVLEKVRFDTLYSFIYSPRPFTEAGAWKERVPLEVSSSRLERLQNRHKEILEEKAKL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCEECHHHHHHHHHHHHHHHHHHHHH
EVGKTHVVLVENRREIDNQIVGFEGRSDTGKFIEVACKEKRNPGELVRVEIVSHSKGRLI
CCCCEEEEEECCCHHHCCCEECCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCCEE
ATAKGD
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA