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The map label for this gene is hemL

Identifier: 187927706

GI number: 187927706

Start: 646030

End: 647331

Strand: Reverse

Name: hemL

Synonym: Rpic_0610

Alternate gene names: 187927706

Gene position: 647331-646030 (Counterclockwise)

Preceding gene: 187927713

Following gene: 187927704

Centisome position: 16.42

GC content: 64.59

Gene sequence:

>1302_bases
ATGTCGACTCCCTCCCGCAGCGCCGCCCTGTTTGAACGTGCCCAGAAGACCATCCCCGGCGGAGTGAACTCCCCGGTACG
CGCCTTCCGCTCGGTGGGCGGCATCCCGCGCTTCATTGCCAAGGCAGCCGGCCCATACTTGTGGGATGCCGACGGCACGC
GCTATATCGACTACGTCGGCTCGTGGGGCCCGATGATCGTCGGGCACGCGCATCCGGAGGTTGTGCGTGCTGTGCAGGAA
GTGGCGGCCGACAGCTTCTCGTTCGGGGCGCCTACCGAGGCTGAAGTCGTCATGGCCGAGGAAATCTGCAAGCTCGTGCC
GTCGATCGAGCAGGTGCGGCTCGTGTCGTCGGGCACCGAAGCAACCATGAGCGCCCTGCGCCTGGCGCGTGGCTTTACCG
GGCGTGACCTGATCGTCAAGTTCGAAGGCTGCTACCACGGTCACGCCGACAGCCTGCTGGTCAAGGCCGGTTCAGGCCTG
CTGACGTTTGCCGATACGACGCAGAACGCACCGTCTTCCACCGGCGTGCCGGAAGACGTGGTCAAGCACACGATGGTGCT
GCCGTACAACGACGTGGCCGCACTGCGCGAGGCGTTTGCCCGCCACGGCAAGGAAATCGCCGCCGTGATCGTCGAGCCAG
TGGCCGGCAACATGAACCTCGTGCGTGGCAGCAACGAATTCCACCAGGCCATGCGCGCGCTGTGTACCGAACACGGCGCC
GTGCTGATCTTTGACGAGGTGATGACGGGCTTTCGCGTGGCGCTCGGGTGCGCGCAGGCGCTGTACGGCATCAAGCCGGA
CCTGACGTGCCTGGGCAAAGTGATTGGCGGCGGCATGCCGGCGGCAGCCTTTGGTGGCCGCCGCGACATCATGGGGTTCC
TGGCGCCTCTGGGCAGCGTCTACCAGGCAGGAACGTTGTCGGGGAATCCGCTTGCGGTGGCGGCTGGGCTGACCACGCTG
CGGCTGATTGCTGCGGAGGGTTTCCATGACCGGCTGGCGACGCAGACACGCAAGCTGGTAGACGGGCTGGCGGAAATCGC
TCGTGAGGTTGGTGTGCCGTTTGCCGCCGACAGCGTGGGCGGCATGTTTGGCATCTACTTCCGCGAGGGCGTGCCGACGA
GCTTTGCTGAAGTGACCAAGAGCGATGTTGGGCGCTTCAATGCGTTCTTCCACGCGATGCTGGATCAGGGTGTTTATCTG
GCGCCGTCTGCGTTTGAGGCCGGATTTGTGTCTTCCACGCATGATGATGCGATTCTGGACGCGACGTTTGAGGCGGCTCG
GAAGGCGTTTAAGGCGGTTTGA

Upstream 100 bases:

>100_bases
CCGGATCTGCGGCAAACCACCACATAATCGCCTCGAACCGCCTTTAAACCGTGCCGCAGCGCCGCTCTGCTCGGCGCCAC
GCTCTCTCCCAACACCCTCC

Downstream 100 bases:

>100_bases
GTTTTTTTTCTTGGGATGGTCTTGGTGGTGCATCCCGGGTTTCATCCCCTGCCGGGGCTGACTCACTTTCTTTGTCTTGC
CAAAGAAAGTAAGCAAAGAA

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSTPSRSAALFERAQKTIPGGVNSPVRAFRSVGGIPRFIAKAAGPYLWDADGTRYIDYVGSWGPMIVGHAHPEVVRAVQE
VAADSFSFGAPTEAEVVMAEEICKLVPSIEQVRLVSSGTEATMSALRLARGFTGRDLIVKFEGCYHGHADSLLVKAGSGL
LTFADTTQNAPSSTGVPEDVVKHTMVLPYNDVAALREAFARHGKEIAAVIVEPVAGNMNLVRGSNEFHQAMRALCTEHGA
VLIFDEVMTGFRVALGCAQALYGIKPDLTCLGKVIGGGMPAAAFGGRRDIMGFLAPLGSVYQAGTLSGNPLAVAAGLTTL
RLIAAEGFHDRLATQTRKLVDGLAEIAREVGVPFAADSVGGMFGIYFREGVPTSFAEVTKSDVGRFNAFFHAMLDQGVYL
APSAFEAGFVSSTHDDAILDATFEAARKAFKAV

Sequences:

>Translated_433_residues
MSTPSRSAALFERAQKTIPGGVNSPVRAFRSVGGIPRFIAKAAGPYLWDADGTRYIDYVGSWGPMIVGHAHPEVVRAVQE
VAADSFSFGAPTEAEVVMAEEICKLVPSIEQVRLVSSGTEATMSALRLARGFTGRDLIVKFEGCYHGHADSLLVKAGSGL
LTFADTTQNAPSSTGVPEDVVKHTMVLPYNDVAALREAFARHGKEIAAVIVEPVAGNMNLVRGSNEFHQAMRALCTEHGA
VLIFDEVMTGFRVALGCAQALYGIKPDLTCLGKVIGGGMPAAAFGGRRDIMGFLAPLGSVYQAGTLSGNPLAVAAGLTTL
RLIAAEGFHDRLATQTRKLVDGLAEIAREVGVPFAADSVGGMFGIYFREGVPTSFAEVTKSDVGRFNAFFHAMLDQGVYL
APSAFEAGFVSSTHDDAILDATFEAARKAFKAV
>Mature_432_residues
STPSRSAALFERAQKTIPGGVNSPVRAFRSVGGIPRFIAKAAGPYLWDADGTRYIDYVGSWGPMIVGHAHPEVVRAVQEV
AADSFSFGAPTEAEVVMAEEICKLVPSIEQVRLVSSGTEATMSALRLARGFTGRDLIVKFEGCYHGHADSLLVKAGSGLL
TFADTTQNAPSSTGVPEDVVKHTMVLPYNDVAALREAFARHGKEIAAVIVEPVAGNMNLVRGSNEFHQAMRALCTEHGAV
LIFDEVMTGFRVALGCAQALYGIKPDLTCLGKVIGGGMPAAAFGGRRDIMGFLAPLGSVYQAGTLSGNPLAVAAGLTTLR
LIAAEGFHDRLATQTRKLVDGLAEIAREVGVPFAADSVGGMFGIYFREGVPTSFAEVTKSDVGRFNAFFHAMLDQGVYLA
PSAFEAGFVSSTHDDAILDATFEAARKAFKAV

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=309, Percent_Identity=29.126213592233, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI37574042, Length=308, Percent_Identity=27.5974025974026, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI226442705, Length=307, Percent_Identity=26.3843648208469, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI284507298, Length=147, Percent_Identity=32.6530612244898, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1786349, Length=427, Percent_Identity=60.1873536299766, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI1789016, Length=364, Percent_Identity=31.8681318681319, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI1787560, Length=428, Percent_Identity=30.8411214953271, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1788044, Length=326, Percent_Identity=32.2085889570552, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1789759, Length=373, Percent_Identity=27.3458445040214, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI145693181, Length=316, Percent_Identity=30.6962025316456, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1786991, Length=388, Percent_Identity=28.0927835051546, Blast_Score=86, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI71992977, Length=391, Percent_Identity=25.3196930946292, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25144271, Length=330, Percent_Identity=25.4545454545455, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI32564660, Length=346, Percent_Identity=25.1445086705202, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25144274, Length=230, Percent_Identity=24.3478260869565, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323470, Length=317, Percent_Identity=29.9684542586751, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6324432, Length=348, Percent_Identity=26.1494252873563, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21357415, Length=327, Percent_Identity=25.9938837920489, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21356575, Length=320, Percent_Identity=29.375, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_RALPJ (B2U6Q7)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001898193.1
- ProteinModelPortal:   B2U6Q7
- SMR:   B2U6Q7
- GeneID:   6287897
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_0610
- HOGENOM:   HBG725944
- OMA:   DKVIKFE
- ProtClustDB:   PRK00062
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 45735; Mature: 45604

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPSRSAALFERAQKTIPGGVNSPVRAFRSVGGIPRFIAKAAGPYLWDADGTRYIDYVG
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHCCCCEECCCCCEEEHHHC
SWGPMIVGHAHPEVVRAVQEVAADSFSFGAPTEAEVVMAEEICKLVPSIEQVRLVSSGTE
CCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCHH
ATMSALRLARGFTGRDLIVKFEGCYHGHADSLLVKAGSGLLTFADTTQNAPSSTGVPEDV
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCHHH
VKHTMVLPYNDVAALREAFARHGKEIAAVIVEPVAGNMNLVRGSNEFHQAMRALCTEHGA
HHCEEECCCHHHHHHHHHHHHCCHHEEHHHHHCCCCCCEEECCCHHHHHHHHHHHHHCCC
VLIFDEVMTGFRVALGCAQALYGIKPDLTCLGKVIGGGMPAAAFGGRRDIMGFLAPLGSV
EEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHH
YQAGTLSGNPLAVAAGLTTLRLIAAEGFHDRLATQTRKLVDGLAEIAREVGVPFAADSVG
HHCCCCCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHC
GMFGIYFREGVPTSFAEVTKSDVGRFNAFFHAMLDQGVYLAPSAFEAGFVSSTHDDAILD
CCEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHH
ATFEAARKAFKAV
HHHHHHHHHHHCC
>Mature Secondary Structure 
STPSRSAALFERAQKTIPGGVNSPVRAFRSVGGIPRFIAKAAGPYLWDADGTRYIDYVG
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHCCCCEECCCCCEEEHHHC
SWGPMIVGHAHPEVVRAVQEVAADSFSFGAPTEAEVVMAEEICKLVPSIEQVRLVSSGTE
CCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCHH
ATMSALRLARGFTGRDLIVKFEGCYHGHADSLLVKAGSGLLTFADTTQNAPSSTGVPEDV
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCHHH
VKHTMVLPYNDVAALREAFARHGKEIAAVIVEPVAGNMNLVRGSNEFHQAMRALCTEHGA
HHCEEECCCHHHHHHHHHHHHCCHHEEHHHHHCCCCCCEEECCCHHHHHHHHHHHHHCCC
VLIFDEVMTGFRVALGCAQALYGIKPDLTCLGKVIGGGMPAAAFGGRRDIMGFLAPLGSV
EEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHH
YQAGTLSGNPLAVAAGLTTLRLIAAEGFHDRLATQTRKLVDGLAEIAREVGVPFAADSVG
HHCCCCCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHC
GMFGIYFREGVPTSFAEVTKSDVGRFNAFFHAMLDQGVYLAPSAFEAGFVSSTHDDAILD
CCEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHH
ATFEAARKAFKAV
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA