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The map label for this gene is gatA [H]

Identifier: 187927611

GI number: 187927611

Start: 548953

End: 550311

Strand: Reverse

Name: gatA [H]

Synonym: Rpic_0511

Alternate gene names: 187927611

Gene position: 550311-548953 (Counterclockwise)

Preceding gene: 187927612

Following gene: 187927610

Centisome position: 13.96

GC content: 70.05

Gene sequence:

>1359_bases
ATGATGACGCCCCGCCCGAACATGATCCGCGCGCTGGCCGCTGAACTGGCAGCCGGTCGCACCACCAGCATCGCGCTGAC
CGAAGCCGCACTGGAGCGCGCCGAATCGCACCGCGCCGCGGGCGGTGCCGCCTATGTGAGCATTGACGTGCAGGCCGCGC
TGGCGATGGCGCGCGCCGCCGATGCCGCGCGTGCGGCGGGCAACGTGCCCTCGCTGCTGGCCGGGCTGCCGGTGTCGATC
AAGGATCTGTTTGACGTGGCCGGCCAGGTGACGGCTGCGGGTTCGCGCGCCCTGGCCGACCAGCCGCGCGCCACCACCGA
TGCCGCCGCCGTGGCACGCCTGCGCGCAGCCGGCGCCGTGCTGCTCGGCCGCACCAACATGAGCGAATTCGCGTTCTCCG
GACTGGGGCTCAACCCGCACTACGGCACGCCGCGCACGCCAGCGGACGGCGCACGCGCAGCGGGCGGCTCCACCTCGGGC
GGCGCCGTCACCGTTGCGGGCGGCATGGCCGTGGCGGCACTGGGTACCGACACCGGCGGCTCGATCCGCATTCCGTCGGC
GTTCTGCAATCTGACGGGTTTCAAGCCCACCGCCCGCCGCGTGCCGATGACGGGCGGCGTGCCGTTGTCCACGTCGCTCG
ATTCCGGCGGCCCGCTCGCCAACTCGGTCGACTGCTGCGCGATCGTCGACGCCGTGTTGAGCGGCGAGTCCGGCAACGAC
GCATTCGACACCACCGCCGTGCCGCTGGCCGGATTGCGTTTCGGTATCACGCAGGACTTTGTCGGCGCGGACCTCGACAA
CACGGTCGCCGTTGCATTCGAACGCGCTGTTGAACGGCTGGAGCGTGCCGGCGCGCACATCGTGCGTTTCGAATTTCCCG
AATTGCTGCAACTGCCGGAGATCAACAGCGGCGGCGGCTTCTCGGCAGCGGAATCCTGGGCATGGCATCGCTCGCTGCTC
GCGCGTGCCGAAGTCCAGTACGACCCGCGCGTGGCCACGCGCATCCGCCGTGGCGAACAGATGAGCGCGGCCGCATACAT
CGACGTGATCAACGCACGCGCACGCATGATTGCCGCCGCACGCAAGCGCCTGGGCAACTTCGATGCATGGCTGATGCCGA
CGGTTGCCATCGTGCCGCCCGAGGTCGCTCCGCTCGAGGTCGATGACGCGCAGTTCTTCCGCATCAATGCGCTCACGCTG
CGCAATCCGAGCGTGATCAATTTCCTGGACGGCTGCGCGCTGACGCTGCCGGTACACGTTGCCGGCGAACTGCCGGTCGG
CCTGTCGCTCTGCGGTCTCGCCAACGACGACGCGCGCATCCTGCGCGTCGGCCGGGCGGTGGAGGCTGCCCTGCGTTGA

Upstream 100 bases:

>100_bases
CCTGATCGACCCCGTGTCGGGCACCGAGCTGCGCCATCGCTATGCTGTCGACACGCTGCCTGTTGAAGGCTGAAGCGCCC
CACCTCACGGAGACCACCGC

Downstream 100 bases:

>100_bases
TCGACTAGCCAGAACATTCTCCGCCTGCGTCCGGGCCACGCAGGCGGTTCCCCAAGCCAAGCCAAAAAGGCGCGCTGCAG
ACGCGCCAGGCCCAACTCAT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MMTPRPNMIRALAAELAAGRTTSIALTEAALERAESHRAAGGAAYVSIDVQAALAMARAADAARAAGNVPSLLAGLPVSI
KDLFDVAGQVTAAGSRALADQPRATTDAAAVARLRAAGAVLLGRTNMSEFAFSGLGLNPHYGTPRTPADGARAAGGSTSG
GAVTVAGGMAVAALGTDTGGSIRIPSAFCNLTGFKPTARRVPMTGGVPLSTSLDSGGPLANSVDCCAIVDAVLSGESGND
AFDTTAVPLAGLRFGITQDFVGADLDNTVAVAFERAVERLERAGAHIVRFEFPELLQLPEINSGGGFSAAESWAWHRSLL
ARAEVQYDPRVATRIRRGEQMSAAAYIDVINARARMIAAARKRLGNFDAWLMPTVAIVPPEVAPLEVDDAQFFRINALTL
RNPSVINFLDGCALTLPVHVAGELPVGLSLCGLANDDARILRVGRAVEAALR

Sequences:

>Translated_452_residues
MMTPRPNMIRALAAELAAGRTTSIALTEAALERAESHRAAGGAAYVSIDVQAALAMARAADAARAAGNVPSLLAGLPVSI
KDLFDVAGQVTAAGSRALADQPRATTDAAAVARLRAAGAVLLGRTNMSEFAFSGLGLNPHYGTPRTPADGARAAGGSTSG
GAVTVAGGMAVAALGTDTGGSIRIPSAFCNLTGFKPTARRVPMTGGVPLSTSLDSGGPLANSVDCCAIVDAVLSGESGND
AFDTTAVPLAGLRFGITQDFVGADLDNTVAVAFERAVERLERAGAHIVRFEFPELLQLPEINSGGGFSAAESWAWHRSLL
ARAEVQYDPRVATRIRRGEQMSAAAYIDVINARARMIAAARKRLGNFDAWLMPTVAIVPPEVAPLEVDDAQFFRINALTL
RNPSVINFLDGCALTLPVHVAGELPVGLSLCGLANDDARILRVGRAVEAALR
>Mature_452_residues
MMTPRPNMIRALAAELAAGRTTSIALTEAALERAESHRAAGGAAYVSIDVQAALAMARAADAARAAGNVPSLLAGLPVSI
KDLFDVAGQVTAAGSRALADQPRATTDAAAVARLRAAGAVLLGRTNMSEFAFSGLGLNPHYGTPRTPADGARAAGGSTSG
GAVTVAGGMAVAALGTDTGGSIRIPSAFCNLTGFKPTARRVPMTGGVPLSTSLDSGGPLANSVDCCAIVDAVLSGESGND
AFDTTAVPLAGLRFGITQDFVGADLDNTVAVAFERAVERLERAGAHIVRFEFPELLQLPEINSGGGFSAAESWAWHRSLL
ARAEVQYDPRVATRIRRGEQMSAAAYIDVINARARMIAAARKRLGNFDAWLMPTVAIVPPEVAPLEVDDAQFFRINALTL
RNPSVINFLDGCALTLPVHVAGELPVGLSLCGLANDDARILRVGRAVEAALR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=320, Percent_Identity=26.875, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI195972892, Length=232, Percent_Identity=30.6034482758621, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI166795287, Length=134, Percent_Identity=38.0597014925373, Blast_Score=72, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17537465, Length=288, Percent_Identity=27.4305555555556, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17543272, Length=276, Percent_Identity=30.4347826086957, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17556264, Length=155, Percent_Identity=38.7096774193548, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=287, Percent_Identity=29.9651567944251, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=220, Percent_Identity=30, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17556276, Length=155, Percent_Identity=37.4193548387097, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=155, Percent_Identity=37.4193548387097, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17538254, Length=213, Percent_Identity=30.9859154929577, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=394, Percent_Identity=29.4416243654822, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6323950, Length=434, Percent_Identity=24.6543778801843, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320448, Length=196, Percent_Identity=30.6122448979592, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648113, Length=505, Percent_Identity=28.3168316831683, Blast_Score=111, Evalue=8e-25,
Organism=Drosophila melanogaster, GI161078093, Length=497, Percent_Identity=24.9496981891348, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI45550774, Length=206, Percent_Identity=31.0679611650485, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648435, Length=206, Percent_Identity=31.0679611650485, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648437, Length=206, Percent_Identity=31.0679611650485, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356731, Length=202, Percent_Identity=33.6633663366337, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24644968, Length=311, Percent_Identity=25.7234726688103, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648441, Length=138, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24648439, Length=138, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24652985, Length=482, Percent_Identity=23.6514522821577, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI19922090, Length=482, Percent_Identity=23.6514522821577, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24652981, Length=482, Percent_Identity=23.6514522821577, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24652983, Length=482, Percent_Identity=23.6514522821577, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 46629; Mature: 46629

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTPRPNMIRALAAELAAGRTTSIALTEAALERAESHRAAGGAAYVSIDVQAALAMARAA
CCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHH
DAARAAGNVPSLLAGLPVSIKDLFDVAGQVTAAGSRALADQPRATTDAAAVARLRAAGAV
HHHHHCCCCHHHHHCCCCCHHHHHHHHCCHHCCCCHHHCCCCCCCHHHHHHHHHHHCCEE
LLGRTNMSEFAFSGLGLNPHYGTPRTPADGARAAGGSTSGGAVTVAGGMAVAALGTDTGG
EEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCC
SIRIPSAFCNLTGFKPTARRVPMTGGVPLSTSLDSGGPLANSVDCCAIVDAVLSGESGND
CEECCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
AFDTTAVPLAGLRFGITQDFVGADLDNTVAVAFERAVERLERAGAHIVRFEFPELLQLPE
CCCCCCCCHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHCCCC
INSGGGFSAAESWAWHRSLLARAEVQYDPRVATRIRRGEQMSAAAYIDVINARARMIAAA
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
RKRLGNFDAWLMPTVAIVPPEVAPLEVDDAQFFRINALTLRNPSVINFLDGCALTLPVHV
HHHHCCCCHHHHCEEEECCCCCCCEEECCCCEEEEEEEEECCCCHHHHHCCCEEEEEEEE
AGELPVGLSLCGLANDDARILRVGRAVEAALR
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMTPRPNMIRALAAELAAGRTTSIALTEAALERAESHRAAGGAAYVSIDVQAALAMARAA
CCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHH
DAARAAGNVPSLLAGLPVSIKDLFDVAGQVTAAGSRALADQPRATTDAAAVARLRAAGAV
HHHHHCCCCHHHHHCCCCCHHHHHHHHCCHHCCCCHHHCCCCCCCHHHHHHHHHHHCCEE
LLGRTNMSEFAFSGLGLNPHYGTPRTPADGARAAGGSTSGGAVTVAGGMAVAALGTDTGG
EEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCC
SIRIPSAFCNLTGFKPTARRVPMTGGVPLSTSLDSGGPLANSVDCCAIVDAVLSGESGND
CEECCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
AFDTTAVPLAGLRFGITQDFVGADLDNTVAVAFERAVERLERAGAHIVRFEFPELLQLPE
CCCCCCCCHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHCCCC
INSGGGFSAAESWAWHRSLLARAEVQYDPRVATRIRRGEQMSAAAYIDVINARARMIAAA
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
RKRLGNFDAWLMPTVAIVPPEVAPLEVDDAQFFRINALTLRNPSVINFLDGCALTLPVHV
HHHHCCCCHHHHCEEEECCCCCCCEEECCCCEEEEEEEEECCCCHHHHHCCCEEEEEEEE
AGELPVGLSLCGLANDDARILRVGRAVEAALR
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA