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The map label for this gene is munIM [H]

Identifier: 187927543

GI number: 187927543

Start: 473700

End: 474980

Strand: Reverse

Name: munIM [H]

Synonym: Rpic_0443

Alternate gene names: 187927543

Gene position: 474980-473700 (Counterclockwise)

Preceding gene: 187927544

Following gene: 187927542

Centisome position: 12.05

GC content: 57.61

Gene sequence:

>1281_bases
ATGAAAACACCAACTGATAAAGCAACACCAGTCGGCGGCTTCGTCATCCACGCAGCCGCCAATCTGTTCCCTGCCATGAA
CGACAGCGAGTTCGAAGAGTTCAAGGAAGACATCCGCCAGAATGGGCAACAGGTTCCCATTCTCGTACAGAACGGGCAAC
TGATCGATGGCCGTCACCGCTATCGCGCCTGCTGCGAGCTGGGCATCGAGCCAAAGGTCGAAGAAGTGCCTGTTGACCAA
TCCATCGAGCGTCTGGTTATCAGCCTTAACCAGCACCGCCGCCATCTGCTCGAGAGCCAGCGCGCCATGATTGCCGCCCG
CCTTGCCAACATCGGTCTTGGGGGCAACCAGCATACGGGCGGGTTATCTCAGCAACAAGCTGCTGACGATCTGGATGTTT
CCGTTGCTTCCGTGCAGCGCGCCAAGTCTGTTCTCGTTCGCGGTACGGACGAGCTGATTCAGGCCGTGGACGCAGGCAAG
GTCGATGTCAGCAATGGTGCCGATCTGGCAAAGCTACCTCAGCCATCTCAACGCAAAGTCATGTCTGACGTCGAGAAGGG
CATTCTGAAGGAGGCCAAGGAGATCCGCAAAGCCATCGCAGCCGAACGCCGTGAGCAATGTCTGGCAGCACTTGAAGTCA
AGCGCGCCAACAGCAAGCCTCTCGACCCAACCAAAGGACCATTCAGCGTGGTACTCGCAGATCCACCATGGAACTATTTA
TCCGAGGCTCAGTTGGGCTACACCACCATGACGCTCGAGGAGATTTGCCAGATGCCTGTCAACGAGCTCGCTGCCGAGGA
CGCCGTGCTATTCCTTTGGTGCTCAGCATCTCTTCCTCAAGAGGCGCTTGACGTGATCAAGGCATGGGGATTCACCTTCA
AGACGCAGGCCATCTGGGACAAGGTGCATCCGGGCATGGGGTCATATTTCCGCATCCAGCACGAGCATCTGATGATCGCT
ACGCGCGGCAATGTTCCCGAGGTTCCAGGCACGGTTCGTTTCGCTTCCGTGTTTACAGAAAAACGCCGTGAGCACAGCCG
TAAGCCTGACTGCGCGTATGAAATGATCGAGGCGATGTATCCCGAGCTTAACAAGATCGAGCTGTTCTGCCGTGGCGAGC
CTCGCACTGGCTGGGCGGGCTGGGGCAATGAATGTGGCAATGGCGAGATCGTGCAGGCTGGCGTCGAGGTTGTGGTCGAT
ACCGATGCCATGCCAGACGCTGCCAACGACGGAGGCGACCCACCTCGCCGTCGTGGTCGTCCACGCAAAGTCATTGCATA
A

Upstream 100 bases:

>100_bases
GACGATTGCCACCATGGCCCCTGAAGTGCTTGACGGTCACGACGGCTATGTCTCATTCGAAATCAACGTAGCCGCAAAAC
AATAACGAGGAGAAGCCGCA

Downstream 100 bases:

>100_bases
CAGGAGGAGATCGATATGGATACAAACAAAAAACGCGAAATCATCAAGGGCAAATTCCCTTGCGAGGTGCCAATCATATT
TTCTGGCATTGCCCCAGGCG

Product: MT-A70 family protein

Products: NA

Alternate protein names: Adenine-specific methyltransferase MunI; M.MunI [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKTPTDKATPVGGFVIHAAANLFPAMNDSEFEEFKEDIRQNGQQVPILVQNGQLIDGRHRYRACCELGIEPKVEEVPVDQ
SIERLVISLNQHRRHLLESQRAMIAARLANIGLGGNQHTGGLSQQQAADDLDVSVASVQRAKSVLVRGTDELIQAVDAGK
VDVSNGADLAKLPQPSQRKVMSDVEKGILKEAKEIRKAIAAERREQCLAALEVKRANSKPLDPTKGPFSVVLADPPWNYL
SEAQLGYTTMTLEEICQMPVNELAAEDAVLFLWCSASLPQEALDVIKAWGFTFKTQAIWDKVHPGMGSYFRIQHEHLMIA
TRGNVPEVPGTVRFASVFTEKRREHSRKPDCAYEMIEAMYPELNKIELFCRGEPRTGWAGWGNECGNGEIVQAGVEVVVD
TDAMPDAANDGGDPPRRRGRPRKVIA

Sequences:

>Translated_426_residues
MKTPTDKATPVGGFVIHAAANLFPAMNDSEFEEFKEDIRQNGQQVPILVQNGQLIDGRHRYRACCELGIEPKVEEVPVDQ
SIERLVISLNQHRRHLLESQRAMIAARLANIGLGGNQHTGGLSQQQAADDLDVSVASVQRAKSVLVRGTDELIQAVDAGK
VDVSNGADLAKLPQPSQRKVMSDVEKGILKEAKEIRKAIAAERREQCLAALEVKRANSKPLDPTKGPFSVVLADPPWNYL
SEAQLGYTTMTLEEICQMPVNELAAEDAVLFLWCSASLPQEALDVIKAWGFTFKTQAIWDKVHPGMGSYFRIQHEHLMIA
TRGNVPEVPGTVRFASVFTEKRREHSRKPDCAYEMIEAMYPELNKIELFCRGEPRTGWAGWGNECGNGEIVQAGVEVVVD
TDAMPDAANDGGDPPRRRGRPRKVIA
>Mature_426_residues
MKTPTDKATPVGGFVIHAAANLFPAMNDSEFEEFKEDIRQNGQQVPILVQNGQLIDGRHRYRACCELGIEPKVEEVPVDQ
SIERLVISLNQHRRHLLESQRAMIAARLANIGLGGNQHTGGLSQQQAADDLDVSVASVQRAKSVLVRGTDELIQAVDAGK
VDVSNGADLAKLPQPSQRKVMSDVEKGILKEAKEIRKAIAAERREQCLAALEVKRANSKPLDPTKGPFSVVLADPPWNYL
SEAQLGYTTMTLEEICQMPVNELAAEDAVLFLWCSASLPQEALDVIKAWGFTFKTQAIWDKVHPGMGSYFRIQHEHLMIA
TRGNVPEVPGTVRFASVFTEKRREHSRKPDCAYEMIEAMYPELNKIELFCRGEPRTGWAGWGNECGNGEIVQAGVEVVVD
TDAMPDAANDGGDPPRRRGRPRKVIA

Specific function: This methylase recognizes the double-stranded sequence CAATTG, causes specific methylation on A-3 on both strands, and protects the DNA from cleavage by the MunI endonuclease [H]

COG id: COG4725

COG function: function code TK; Transcriptional activator, adenine-specific DNA methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MT-A70-like family [H]

Homologues:

Organism=Homo sapiens, GI21361827, Length=170, Percent_Identity=30.5882352941176, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6321246, Length=181, Percent_Identity=29.2817679558011, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21355141, Length=170, Percent_Identity=31.1764705882353, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR007757 [H]

Pfam domain/function: PF05063 MT-A70 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 47020; Mature: 47020

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00092 N6_MTASE ; PS51143 MT_A70

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTPTDKATPVGGFVIHAAANLFPAMNDSEFEEFKEDIRQNGQQVPILVQNGQLIDGRHR
CCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCEECCHHH
YRACCELGIEPKVEEVPVDQSIERLVISLNQHRRHLLESQRAMIAARLANIGLGGNQHTG
HHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GLSQQQAADDLDVSVASVQRAKSVLVRGTDELIQAVDAGKVDVSNGADLAKLPQPSQRKV
CCCHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEECCCCCCHHHCCCCHHHHH
MSDVEKGILKEAKEIRKAIAAERREQCLAALEVKRANSKPLDPTKGPFSVVLADPPWNYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHH
SEAQLGYTTMTLEEICQMPVNELAAEDAVLFLWCSASLPQEALDVIKAWGFTFKTQAIWD
HHCCCCHHHHHHHHHHCCCHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEHHHHHH
KVHPGMGSYFRIQHEHLMIATRGNVPEVPGTVRFASVFTEKRREHSRKPDCAYEMIEAMY
HHCCCCCCEEEEEECEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
PELNKIELFCRGEPRTGWAGWGNECGNGEIVQAGVEVVVDTDAMPDAANDGGDPPRRRGR
CCCCEEEEEECCCCCCCCCCCCCCCCCCCEEECCEEEEEECCCCCCCCCCCCCCHHHCCC
PRKVIA
CCCCCC
>Mature Secondary Structure
MKTPTDKATPVGGFVIHAAANLFPAMNDSEFEEFKEDIRQNGQQVPILVQNGQLIDGRHR
CCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCEECCHHH
YRACCELGIEPKVEEVPVDQSIERLVISLNQHRRHLLESQRAMIAARLANIGLGGNQHTG
HHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GLSQQQAADDLDVSVASVQRAKSVLVRGTDELIQAVDAGKVDVSNGADLAKLPQPSQRKV
CCCHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEECCCCCCHHHCCCCHHHHH
MSDVEKGILKEAKEIRKAIAAERREQCLAALEVKRANSKPLDPTKGPFSVVLADPPWNYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHH
SEAQLGYTTMTLEEICQMPVNELAAEDAVLFLWCSASLPQEALDVIKAWGFTFKTQAIWD
HHCCCCHHHHHHHHHHCCCHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEHHHHHH
KVHPGMGSYFRIQHEHLMIATRGNVPEVPGTVRFASVFTEKRREHSRKPDCAYEMIEAMY
HHCCCCCCEEEEEECEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
PELNKIELFCRGEPRTGWAGWGNECGNGEIVQAGVEVVVDTDAMPDAANDGGDPPRRRGR
CCCCEEEEEECCCCCCCCCCCCCCCCCCCEEECCEEEEEECCCCCCCCCCCCCCHHHCCC
PRKVIA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8181741 [H]