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The map label for this gene is dusB [H]

Identifier: 187927485

GI number: 187927485

Start: 404655

End: 405722

Strand: Reverse

Name: dusB [H]

Synonym: Rpic_0382

Alternate gene names: 187927485

Gene position: 405722-404655 (Counterclockwise)

Preceding gene: 187927486

Following gene: 187927484

Centisome position: 10.29

GC content: 66.29

Gene sequence:

>1068_bases
TTGCAGATCGGACCGCATACCCTTCGCAACAACCTGTTCGTCGCTCCCATGGCGGGTGTGACGGATCGCCCGTTCCGGCA
GCTCTGCAAGCGGCTGGGCGCGGGCTATGCCGTGTCCGAGATGGTCGCGTCCAACGCGCAGCTCTGGAAAAGCGAGAAGA
CCATGCGGCGCGCCAACCACACGGGCGAGGTCGAGCCGATCGCCGTGCAGATTGCCGGCGCCGAGCCGATGATGATGGCC
GAGGCCGCGCGCTACAACGTCGATCGCGGCGCGCAAATCATCGACATCAACATGGGCTGCCCGGCCAAGAAGGTGTGCAA
CGTGGCCGCTGGTTCTGCCCTGCTGCAGAACGAGCCGCTGGTCCAGCGCATTGTCGAGGCCGTGGTGGGTGCGGTCGGCG
ACCGCGTGCCCGTGACGCTCAAGATCCGTACCGGTTGGGACCGCGAACACCGCAACGCATTGAGCGTGGCGCGCATTGCA
CAGGACGCCGGCATCAGCATGCTGACCGTGCATGGCCGCACGCGCGCCGATCTGTATCACGGTGAAGCGGAATACGACAC
CATCGCCGCGGTCAAGGCGTCGGTGCGCATTCCCGTGGTGGCCAACGGCGACATCACCACGCCGGCCAAGGCCAAGCACG
TGCTGGCCGTGACCGGCGCCGACGCCATCATGATCGGCCGCGCGGCGCAGGGGCGCCCGTGGCTGTTCCGCGAGATCGAG
CATTTCCTGCAGACCGGCACGCTGCTGCCGGCGCCGGAGGTGGAAGAGATTCGCGGCATCATGAACGCGCATCTGGAAGA
GCACTACGCGTTCTATGGCGAATACACCGGCGTGCGCACCGCACGCAAGCACATCGCCTGGTACACGCGCGGGTTGGCGG
GGGCCAACCTGTTCCGCCACCGCATGAACACCATCGAGGACACCGCGGCCCAGTTGGCCGCCGTCAATGCGTTCTTCGAC
GAGCAGCGTGCGCTGTCCGACCGTCTGGTCTACGAAGACCAGGCGACCGGTGGCGACCACCCCAGGACGGGCAACAACGA
CAACAACAAGGAACTGCTTGCCGCATGA

Upstream 100 bases:

>100_bases
CCTCGCATTTTTCACTCCCGTCGCTCCGCCCGATCATCATTCGACTCTGGGCGGAAGGGGTGATTTTTCGTTTCTTTCAG
CCGCGTCTTCCTCGACAGCC

Downstream 100 bases:

>100_bases
GCCGCAACCCGATCGAACGTTGCATCCGCGACAGCCTGGATGCGTATTACCGTGACCTGGACGGCGAGAACCCGTCCAAC
GTCTACGACATGGTGCTGCA

Product: TIM-barrel protein, nifR3 family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MQIGPHTLRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSEKTMRRANHTGEVEPIAVQIAGAEPMMMA
EAARYNVDRGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVQRIVEAVVGAVGDRVPVTLKIRTGWDREHRNALSVARIA
QDAGISMLTVHGRTRADLYHGEAEYDTIAAVKASVRIPVVANGDITTPAKAKHVLAVTGADAIMIGRAAQGRPWLFREIE
HFLQTGTLLPAPEVEEIRGIMNAHLEEHYAFYGEYTGVRTARKHIAWYTRGLAGANLFRHRMNTIEDTAAQLAAVNAFFD
EQRALSDRLVYEDQATGGDHPRTGNNDNNKELLAA

Sequences:

>Translated_355_residues
MQIGPHTLRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSEKTMRRANHTGEVEPIAVQIAGAEPMMMA
EAARYNVDRGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVQRIVEAVVGAVGDRVPVTLKIRTGWDREHRNALSVARIA
QDAGISMLTVHGRTRADLYHGEAEYDTIAAVKASVRIPVVANGDITTPAKAKHVLAVTGADAIMIGRAAQGRPWLFREIE
HFLQTGTLLPAPEVEEIRGIMNAHLEEHYAFYGEYTGVRTARKHIAWYTRGLAGANLFRHRMNTIEDTAAQLAAVNAFFD
EQRALSDRLVYEDQATGGDHPRTGNNDNNKELLAA
>Mature_355_residues
MQIGPHTLRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSEKTMRRANHTGEVEPIAVQIAGAEPMMMA
EAARYNVDRGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVQRIVEAVVGAVGDRVPVTLKIRTGWDREHRNALSVARIA
QDAGISMLTVHGRTRADLYHGEAEYDTIAAVKASVRIPVVANGDITTPAKAKHVLAVTGADAIMIGRAAQGRPWLFREIE
HFLQTGTLLPAPEVEEIRGIMNAHLEEHYAFYGEYTGVRTARKHIAWYTRGLAGANLFRHRMNTIEDTAAQLAAVNAFFD
EQRALSDRLVYEDQATGGDHPRTGNNDNNKELLAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=239, Percent_Identity=33.4728033472803, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI239788462, Length=239, Percent_Identity=33.4728033472803, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI31742496, Length=225, Percent_Identity=32.4444444444444, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI40807366, Length=230, Percent_Identity=32.1739130434783, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI8923374, Length=160, Percent_Identity=31.875, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1789660, Length=320, Percent_Identity=58.125, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1788462, Length=251, Percent_Identity=32.2709163346614, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI145693211, Length=303, Percent_Identity=25.7425742574257, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25144369, Length=224, Percent_Identity=34.375, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17543114, Length=242, Percent_Identity=28.5123966942149, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17507177, Length=233, Percent_Identity=33.9055793991416, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17510279, Length=263, Percent_Identity=27.3764258555133, Blast_Score=74, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6323433, Length=263, Percent_Identity=29.277566539924, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323437, Length=228, Percent_Identity=28.0701754385965, Blast_Score=89, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6323560, Length=237, Percent_Identity=30.379746835443, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24585320, Length=290, Percent_Identity=29.6551724137931, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24580595, Length=251, Percent_Identity=29.8804780876494, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI19920448, Length=251, Percent_Identity=29.8804780876494, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI19921524, Length=227, Percent_Identity=28.1938325991189, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI45549423, Length=251, Percent_Identity=28.6852589641434, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38821; Mature: 38821

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGPHTLRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSEKTMRRANH
CCCCCHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCC
TGEVEPIAVQIAGAEPMMMAEAARYNVDRGAQIIDINMGCPAKKVCNVAAGSALLQNEPL
CCCCCEEEEEEECCCCHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHCCCHH
VQRIVEAVVGAVGDRVPVTLKIRTGWDREHRNALSVARIAQDAGISMLTVHGRTRADLYH
HHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHEEC
GEAEYDTIAAVKASVRIPVVANGDITTPAKAKHVLAVTGADAIMIGRAAQGRPWLFREIE
CCCCHHHHHEEHEEEEEEEEECCCCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHHHHH
HFLQTGTLLPAPEVEEIRGIMNAHLEEHYAFYGEYTGVRTARKHIAWYTRGLAGANLFRH
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHH
RMNTIEDTAAQLAAVNAFFDEQRALSDRLVYEDQATGGDHPRTGNNDNNKELLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCEEECC
>Mature Secondary Structure
MQIGPHTLRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSEKTMRRANH
CCCCCHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCC
TGEVEPIAVQIAGAEPMMMAEAARYNVDRGAQIIDINMGCPAKKVCNVAAGSALLQNEPL
CCCCCEEEEEEECCCCHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHCCCHH
VQRIVEAVVGAVGDRVPVTLKIRTGWDREHRNALSVARIAQDAGISMLTVHGRTRADLYH
HHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHEEC
GEAEYDTIAAVKASVRIPVVANGDITTPAKAKHVLAVTGADAIMIGRAAQGRPWLFREIE
CCCCHHHHHEEHEEEEEEEEECCCCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHHHHH
HFLQTGTLLPAPEVEEIRGIMNAHLEEHYAFYGEYTGVRTARKHIAWYTRGLAGANLFRH
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHH
RMNTIEDTAAQLAAVNAFFDEQRALSDRLVYEDQATGGDHPRTGNNDNNKELLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]