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The map label for this gene is yafJ [C]

Identifier: 187927464

GI number: 187927464

Start: 386050

End: 386880

Strand: Reverse

Name: yafJ [C]

Synonym: Rpic_0361

Alternate gene names: 187927464

Gene position: 386880-386050 (Counterclockwise)

Preceding gene: 187927468

Following gene: 187927456

Centisome position: 9.81

GC content: 62.21

Gene sequence:

>831_bases
ATGTGCCAGCTGCTGGGCATGAACTGCGCCGAACCGACCGATGTGACGTTCTCGTTCACCGGGTTCGCCGCGCGCGGTGG
CGTCACCGACCACCATGCCGACGGCTTTGGCGTCGCCTTCTTCGAAGACAAGGCCTGCCGCCTGTTCATCGACAATCAGT
CGGCCGGCACCTCGCCGGTGGCGGAACTGGTCAAGCGCTACCCGATCAAGTCCAAGAACGTCATCTCGCACATCCGCAAG
GCGACGCAGGGCACGGTGCGGCTGGAAAACTGCCACCCGTTCATGCGGGAGCTGTGGGGCCGGCATTGGATCTTTGCGCA
CAACGGCGACCTGAAGAACTTCTCGCCGTTTCTCTCGGGCGTCTACCAGCCCGTGGGCGATACCGACAGCGAACTCGCCT
TCTGTTTCATCATGCAGGCGCTGCGCAAGCGCTTTCCGGGGTCGCAGCCACCGCTTAACGAGCTGTTCTACGCGCTGTCC
GACATCACCAAGGAAATCACCCGCTACGGTGTGTTCAATTTCTTGCTCTGCAACGGGCAGGCGCAGTTCGCGCATTGCTC
AACGCGGCTGTATTACATCGTGCGGCAGTGGCCGTTCTCGACGGCGCATTTGATCGACGCCGACATGACGATCGACTTCG
CCAAATACACCACGCCCGCCGATCGCGTGGCTGTCATCGCCACCGCACCGCTCACCGACAACGAAGTCTGGACGCAATTC
GCGCCGGGCGAGCTGCTGATGTTCGAATCGGGCCAAGCCACGATGGCGACCAAGGTGCCGATTCCGGAAGCCGTGCAGAT
CGCCAACGCGGCCAATACGGCTTGCACCTGA

Upstream 100 bases:

>100_bases
CCCGGCAGCGGGGCGCGGCGCCGTGAAGACGAAGGCGCTTGGCACCCCCGGCAGCCGGCAGGATAATGGTTATTTTATTG
CATCCGACTTTTCCCCTACC

Downstream 100 bases:

>100_bases
CATGATCATCGCCCAACAAAAAGCCGCTCCTGAAAGAGCGGCTTTTTGTTGCCTCCAACCCGGCGCTTAAATCGGTTCAC
AATCCGTAGCGAGCGGCCCG

Product: glutamine amidotransferase class-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MCQLLGMNCAEPTDVTFSFTGFAARGGVTDHHADGFGVAFFEDKACRLFIDNQSAGTSPVAELVKRYPIKSKNVISHIRK
ATQGTVRLENCHPFMRELWGRHWIFAHNGDLKNFSPFLSGVYQPVGDTDSELAFCFIMQALRKRFPGSQPPLNELFYALS
DITKEITRYGVFNFLLCNGQAQFAHCSTRLYYIVRQWPFSTAHLIDADMTIDFAKYTTPADRVAVIATAPLTDNEVWTQF
APGELLMFESGQATMATKVPIPEAVQIANAANTACT

Sequences:

>Translated_276_residues
MCQLLGMNCAEPTDVTFSFTGFAARGGVTDHHADGFGVAFFEDKACRLFIDNQSAGTSPVAELVKRYPIKSKNVISHIRK
ATQGTVRLENCHPFMRELWGRHWIFAHNGDLKNFSPFLSGVYQPVGDTDSELAFCFIMQALRKRFPGSQPPLNELFYALS
DITKEITRYGVFNFLLCNGQAQFAHCSTRLYYIVRQWPFSTAHLIDADMTIDFAKYTTPADRVAVIATAPLTDNEVWTQF
APGELLMFESGQATMATKVPIPEAVQIANAANTACT
>Mature_276_residues
MCQLLGMNCAEPTDVTFSFTGFAARGGVTDHHADGFGVAFFEDKACRLFIDNQSAGTSPVAELVKRYPIKSKNVISHIRK
ATQGTVRLENCHPFMRELWGRHWIFAHNGDLKNFSPFLSGVYQPVGDTDSELAFCFIMQALRKRFPGSQPPLNELFYALS
DITKEITRYGVFNFLLCNGQAQFAHCSTRLYYIVRQWPFSTAHLIDADMTIDFAKYTTPADRVAVIATAPLTDNEVWTQF
APGELLMFESGQATMATKVPIPEAVQIANAANTACT

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=252, Percent_Identity=49.2063492063492, Blast_Score=271, Evalue=5e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 30712; Mature: 30712

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCQLLGMNCAEPTDVTFSFTGFAARGGVTDHHADGFGVAFFEDKACRLFIDNQSAGTSPV
CCEEECCCCCCCCCEEEEEECEEECCCCCCCCCCCEEEEEEECCEEEEEEECCCCCCCHH
AELVKRYPIKSKNVISHIRKATQGTVRLENCHPFMRELWGRHWIFAHNGDLKNFSPFLSG
HHHHHHCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHCCCEEEEEECCCCCHHHHHHHH
VYQPVGDTDSELAFCFIMQALRKRFPGSQPPLNELFYALSDITKEITRYGVFNFLLCNGQ
HHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCC
AQFAHCSTRLYYIVRQWPFSTAHLIDADMTIDFAKYTTPADRVAVIATAPLTDNEVWTQF
CCCHHHHHEEEEEEECCCCCCEEEEECCCEEEHHHCCCCCHHEEEEEECCCCCCCCEEEE
APGELLMFESGQATMATKVPIPEAVQIANAANTACT
CCCEEEEEECCCEEEEEECCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MCQLLGMNCAEPTDVTFSFTGFAARGGVTDHHADGFGVAFFEDKACRLFIDNQSAGTSPV
CCEEECCCCCCCCCEEEEEECEEECCCCCCCCCCCEEEEEEECCEEEEEEECCCCCCCHH
AELVKRYPIKSKNVISHIRKATQGTVRLENCHPFMRELWGRHWIFAHNGDLKNFSPFLSG
HHHHHHCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHCCCEEEEEECCCCCHHHHHHHH
VYQPVGDTDSELAFCFIMQALRKRFPGSQPPLNELFYALSDITKEITRYGVFNFLLCNGQ
HHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCC
AQFAHCSTRLYYIVRQWPFSTAHLIDADMTIDFAKYTTPADRVAVIATAPLTDNEVWTQF
CCCHHHHHEEEEEEECCCCCCEEEEECCCEEEHHHCCCCCHHEEEEEECCCCCCCCEEEE
APGELLMFESGQATMATKVPIPEAVQIANAANTACT
CCCEEEEEECCCEEEEEECCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]