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The map label for this gene is ubiB [H]

Identifier: 187927439

GI number: 187927439

Start: 356202

End: 357779

Strand: Direct

Name: ubiB [H]

Synonym: Rpic_0336

Alternate gene names: 187927439

Gene position: 356202-357779 (Clockwise)

Preceding gene: 187927438

Following gene: 187927440

Centisome position: 9.03

GC content: 64.45

Gene sequence:

>1578_bases
ATGACGCGCTTCTTCCGGCTGGGCAAGATCGTTTTCGTCATCCTCTATTACGGGCTGGATCAGCTCGCGTTGTCGGGCTT
CAAGAGCCGCCGCATTCGCGCGCTGGTCTGGCTGCTCACGCTGGGCCGCAAGCCGAAACTCCCGCGTGGCGAGCGCCTGC
GCCTGGCGTTGGAGCAGCTTGGGCCCATCTTCGTGAAGTTCGGGCAGGTGCTGTCGACGCGGCGCGATCTGCTGCCGCCA
GATGTGGCCGACGAACTGGCCAAGCTGCAGGATCGTGTGCCGCCGTTCGACCCGAAGGTGGCCGCCGCCATCGTGGAGAA
ATCGCTCGGCAAGCCGCTCTCGGCGCTGTTCCATCGTTTCGATCATCACCCCGTGGCGAGCGCGTCGATTGCACAGGTGC
ATTTCGCCACGTTGCGCGGCGGCCCCTATGATGGTCGCGAGGTGGCGGTCAAGGTCTTGCGCCCGGGCATGCTGCCTGTG
ATCGACAGCGACCTCGCGCTCATGCGGGATCTCGCTACCTGGATCGAAAAGCTGTGGGCCGACGCCAAGCGCCTGAAGCC
GCGCGAGGTGGTCGCCGAGTTCGACAAGTACCTGCACGACGAGCTGGACCTGATGCGCGAGGCGGCCAACGCCAGCCAGC
TGCGCCGCAATTTTGCCAAGTCCGATCTGTTGCTGGTGCCCGAGGTGTTCTGGGATTGGTGCACGTCTGAAGTGTTCGTG
ATGGAGCGCATGCACGGCATGCGGATCTCGCACACCGAAGAGCTGCGTGCCGCCGGTGTCGACATGCACAAGCTCGCCCG
CGACGGCGTGGAGATCTTCTTCACGCAGGTCTTTCGCGACGGCTTTTTCCACGCCGACATGCACCCGGGCAACATCCTCG
TGAGCGTGGCACCCGAGACGCTGGGCCGCTATATCGCGCTGGATTTCGGCATCGTCGGGGCGCTGTCGGAGTTCGACAAA
AACTACCTCGCGCAGAACTTCCTGGCGTTTTTCCAGCGCGACTACCACCGCGTGGCGGTGCTGCATATCGAGTCCGGCTG
GGCGCCGGAAGAGACGCGCGTGGAAGAGCTGGAAGGCGCCATCCGCGCATGCTGCGAACCGTATTTCGATCGGCCGCTGG
GCGAGATCTCGCTCGGCCTGGTGCTGATGCGACTGTTCCAGACCTCGCGCCGCTTCAACGTGGAAGTGCAGCCGCAGCTC
GTGCTGCTGCAGAAGACGCTGCTCAACGTGGAAGGCCTTGGGCGCCAGCTCGATCCCGATCTGGACCTGTGGAAGACGGC
CAAGCCCTTCCTCGAGCGCTGGATGCACGAGCAGATCGGCTGGCGCGGTTTTGTCGAGCGCCTGAAGGTCGAGGCGCCGC
AATGGGCCAACAAGCTGCCGGACTTCCCGCGTCTGGTGCACCAGATTCTGGATCGCCACTCCCGCGAGGACGGCAATGCG
CAAACTGCGGCGCTCACCGCGCTGCTTGCCGAGCAAAAGCGCACGAACCGGCTGTTGTCTGCCGCTCTGCTGTTCGTTGG
TGGCTTCGCAGTCGGCATTGTCGCGACGCATGTGCTGGCCTGGATGGCCCGTTACTGA

Upstream 100 bases:

>100_bases
CCGCACTGCGTGACGCCGAAGCGCGTCTGGTCAAACGGCTGGAGCGGCTCGAAAAGCTCCCGCGACCGGCCACCCGGACC
GCACGTCAGGGTGAATCGGC

Downstream 100 bases:

>100_bases
TCCGCCTTCACTTTCCCAGCGCCATGGCCCAACCGCCCGTCTTCCAATCCCGTGACGCCGCCGATCCCGCCTTCTGGGAC
GAGCGCTTCACCCGCGAGCA

Product: putative ubiquinone biosynthesis protein UbiB

Products: 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MTRFFRLGKIVFVILYYGLDQLALSGFKSRRIRALVWLLTLGRKPKLPRGERLRLALEQLGPIFVKFGQVLSTRRDLLPP
DVADELAKLQDRVPPFDPKVAAAIVEKSLGKPLSALFHRFDHHPVASASIAQVHFATLRGGPYDGREVAVKVLRPGMLPV
IDSDLALMRDLATWIEKLWADAKRLKPREVVAEFDKYLHDELDLMREAANASQLRRNFAKSDLLLVPEVFWDWCTSEVFV
MERMHGMRISHTEELRAAGVDMHKLARDGVEIFFTQVFRDGFFHADMHPGNILVSVAPETLGRYIALDFGIVGALSEFDK
NYLAQNFLAFFQRDYHRVAVLHIESGWAPEETRVEELEGAIRACCEPYFDRPLGEISLGLVLMRLFQTSRRFNVEVQPQL
VLLQKTLLNVEGLGRQLDPDLDLWKTAKPFLERWMHEQIGWRGFVERLKVEAPQWANKLPDFPRLVHQILDRHSREDGNA
QTAALTALLAEQKRTNRLLSAALLFVGGFAVGIVATHVLAWMARY

Sequences:

>Translated_525_residues
MTRFFRLGKIVFVILYYGLDQLALSGFKSRRIRALVWLLTLGRKPKLPRGERLRLALEQLGPIFVKFGQVLSTRRDLLPP
DVADELAKLQDRVPPFDPKVAAAIVEKSLGKPLSALFHRFDHHPVASASIAQVHFATLRGGPYDGREVAVKVLRPGMLPV
IDSDLALMRDLATWIEKLWADAKRLKPREVVAEFDKYLHDELDLMREAANASQLRRNFAKSDLLLVPEVFWDWCTSEVFV
MERMHGMRISHTEELRAAGVDMHKLARDGVEIFFTQVFRDGFFHADMHPGNILVSVAPETLGRYIALDFGIVGALSEFDK
NYLAQNFLAFFQRDYHRVAVLHIESGWAPEETRVEELEGAIRACCEPYFDRPLGEISLGLVLMRLFQTSRRFNVEVQPQL
VLLQKTLLNVEGLGRQLDPDLDLWKTAKPFLERWMHEQIGWRGFVERLKVEAPQWANKLPDFPRLVHQILDRHSREDGNA
QTAALTALLAEQKRTNRLLSAALLFVGGFAVGIVATHVLAWMARY
>Mature_524_residues
TRFFRLGKIVFVILYYGLDQLALSGFKSRRIRALVWLLTLGRKPKLPRGERLRLALEQLGPIFVKFGQVLSTRRDLLPPD
VADELAKLQDRVPPFDPKVAAAIVEKSLGKPLSALFHRFDHHPVASASIAQVHFATLRGGPYDGREVAVKVLRPGMLPVI
DSDLALMRDLATWIEKLWADAKRLKPREVVAEFDKYLHDELDLMREAANASQLRRNFAKSDLLLVPEVFWDWCTSEVFVM
ERMHGMRISHTEELRAAGVDMHKLARDGVEIFFTQVFRDGFFHADMHPGNILVSVAPETLGRYIALDFGIVGALSEFDKN
YLAQNFLAFFQRDYHRVAVLHIESGWAPEETRVEELEGAIRACCEPYFDRPLGEISLGLVLMRLFQTSRRFNVEVQPQLV
LLQKTLLNVEGLGRQLDPDLDLWKTAKPFLERWMHEQIGWRGFVERLKVEAPQWANKLPDFPRLVHQILDRHSREDGNAQ
TAALTALLAEQKRTNRLLSAALLFVGGFAVGIVATHVLAWMARY

Specific function: Required, probably indirectly, for the hydroxylation of 2-octaprenylphenol to 2-octaprenyl-6-hydroxy-phenol, the fourth step in ubiquinone biosynthesis [H]

COG id: COG0661

COG function: function code R; Predicted unusual protein kinase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ABC1 family. UbiB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40254938, Length=261, Percent_Identity=32.5670498084291, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI41393593, Length=274, Percent_Identity=31.3868613138686, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI32261307, Length=316, Percent_Identity=29.4303797468354, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI217035081, Length=209, Percent_Identity=33.9712918660287, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI34147522, Length=262, Percent_Identity=29.0076335877863, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI27363457, Length=283, Percent_Identity=27.208480565371, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI2367309, Length=470, Percent_Identity=54.468085106383, Blast_Score=498, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI17559152, Length=278, Percent_Identity=27.6978417266187, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32565180, Length=257, Percent_Identity=27.6264591439689, Blast_Score=69, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6325148, Length=441, Percent_Identity=26.7573696145125, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323282, Length=254, Percent_Identity=28.3464566929134, Blast_Score=97, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6321319, Length=205, Percent_Identity=28.780487804878, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI22024280, Length=255, Percent_Identity=29.4117647058824, Blast_Score=122, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24662575, Length=259, Percent_Identity=30.5019305019305, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004147
- InterPro:   IPR011009
- InterPro:   IPR000719
- InterPro:   IPR010232 [H]

Pfam domain/function: PF03109 ABC1 [H]

EC number: NA

Molecular weight: Translated: 59955; Mature: 59823

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS50011 PROTEIN_KINASE_DOM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRFFRLGKIVFVILYYGLDQLALSGFKSRRIRALVWLLTLGRKPKLPRGERLRLALEQL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
GPIFVKFGQVLSTRRDLLPPDVADELAKLQDRVPPFDPKVAAAIVEKSLGKPLSALFHRF
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHH
DHHPVASASIAQVHFATLRGGPYDGREVAVKVLRPGMLPVIDSDLALMRDLATWIEKLWA
CCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHCHHHHHHHHHHHHHHHHHH
DAKRLKPREVVAEFDKYLHDELDLMREAANASQLRRNFAKSDLLLVPEVFWDWCTSEVFV
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHH
MERMHGMRISHTEELRAAGVDMHKLARDGVEIFFTQVFRDGFFHADMHPGNILVSVAPET
HHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECHHH
LGRYIALDFGIVGALSEFDKNYLAQNFLAFFQRDYHRVAVLHIESGWAPEETRVEELEGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHH
IRACCEPYFDRPLGEISLGLVLMRLFQTSRRFNVEVQPQLVLLQKTLLNVEGLGRQLDPD
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEECHHHHHHHHHHHHHHHCCCCCCCC
LDLWKTAKPFLERWMHEQIGWRGFVERLKVEAPQWANKLPDFPRLVHQILDRHSREDGNA
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCH
QTAALTALLAEQKRTNRLLSAALLFVGGFAVGIVATHVLAWMARY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 2-octaprenylphenol; O2 [C]

Specific reaction: 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]