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The map label for this gene is rpoN [H]

Identifier: 187927370

GI number: 187927370

Start: 277725

End: 279236

Strand: Reverse

Name: rpoN [H]

Synonym: Rpic_0263

Alternate gene names: 187927370

Gene position: 279236-277725 (Counterclockwise)

Preceding gene: 187927371

Following gene: 187927369

Centisome position: 7.08

GC content: 60.32

Gene sequence:

>1512_bases
ATGAAACAGTCGCTCCAGCTCCGTCTCTCTCAGCATCTCGCGCTCACGCCGCAGTTGCAGCAATCGATCCGACTGCTGCA
GTTGTCGACCATCGAACTGCAGCAGGAAGTTGAACAGGCCCTGACCGAAAACCCGCTGCTGGAGCGGGAAAACGAGTGGC
TCGACACCTCCGCCCGCATTGGAGCGGACGGCTCCTTGAACGCGCTGCAAAGCAACAGCGCGACGCCCCTGCCCACCGAA
TCGCCTCCGCCCGCACCCAACGGCGGCGACAGCAGCGACAGCTCGGACAGCAGCTACGAAAACGGCCACGACAACGGTGC
CGATTTCGACAGCGATTACGGCTCCGACCGCTCCGACTGGAGCCTCGACGACTTCGCCCGCAAGCCGCAATCCGACGAAG
ACGATCGCGCGCCACTGCAGTTGCGCGAAGCCGAGCAAAGCCTGCGCGAATTCCTCATGGAACAACTCGCGCCGCTCAAG
CTCTCGATGCGCGACAAGGGCCTCGTCATCTTCCTGATCGAATCGCTCGACGACGAAGGTTATCTCTCAGGCACGCTCGA
CGAAATCCTCGCAGACCTGCCGCCCGAACTCGAAGTCGAACTCGACGAACTGCAAGCCGCGCTGCGCATGCTGCAGAGCT
TTGATCCGCCCGGCATTGGCGCGCGCTCAGCGGCCGAATGCCTGTCGTTGCAATTGCATCGGCTCGATTCGCCAGCGAAA
GGGCTGGCGCTCATCATCGTTAATCAGCACCTCGAACTGCTGGCCGCGCGCGACTACACGCGCTTGAAGAAGGCATTGTG
CGTCGACGAACCCACACTGAAAGCTGCGCATGAGCTGATTCGTTCACTGGCACCGTTTCCGGGTCATGCATTCGGCCGCG
CCGAAGCGGATTTCGTGGTGCCCGACGTGATTGTGCGCAAGACAAGCGCAGGCTGGATGGCGCAACTGAACCCCGACGTC
ATGCCGCGGCTGCGCATCAACGACATGTACGCGCAGATCCTGCGCAGCAGCCGCGGTGAATCGGGCGCTGCCAATCTGCA
GCAGAAGCTGCAGGAAGCACGTTGGCTGATCAAGAACATCCAGCAGCGCTTCGATACGATCCTGCGTGTCTCGCAAGCGA
TTGTCGAGCGTCAAAAGAGCTTCTTCACGCACGGCGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACA
CTGGGTCTACACGAGTCCACGATCTCCCGTGTGACAACGAACAAATACATGGCGACCCCGATGGGGACGTTCGAGTTGAA
GTACTTCTTCGGCAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCGTCCACTGCGATACGCGCGCTCATCAAGCAAC
TTGTAGGAGCCGAAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTG
GCCCGTCGGACGGTGGCCAAATACCGCGAAGCCCTCAAGATCCCTGCAGTGAATTTGCGCAAGTCTTTATAG

Upstream 100 bases:

>100_bases
GTAAGCCGCGCTGCACGCTTACGCCTTAAGGACGTGGCAAAGGCGCACCTTAAAGCAAAATCCGTTCCATCGACGAAGTG
TTGGGCATAGAATAGCCCGC

Downstream 100 bases:

>100_bases
CCGCATCGCGGGCAGTTCCGGTGCGGCCCATCTTGAGAAGGAGAACCGCTATGAACTTCAAACTCAGTGGACACCATCTG
GACATCACGCCTGCTTTGCG

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSLNALQSNSATPLPTE
SPPPAPNGGDSSDSSDSSYENGHDNGADFDSDYGSDRSDWSLDDFARKPQSDEDDRAPLQLREAEQSLREFLMEQLAPLK
LSMRDKGLVIFLIESLDDEGYLSGTLDEILADLPPELEVELDELQAALRMLQSFDPPGIGARSAAECLSLQLHRLDSPAK
GLALIIVNQHLELLAARDYTRLKKALCVDEPTLKAAHELIRSLAPFPGHAFGRAEADFVVPDVIVRKTSAGWMAQLNPDV
MPRLRINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADT
LGLHESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVV
ARRTVAKYREALKIPAVNLRKSL

Sequences:

>Translated_503_residues
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSLNALQSNSATPLPTE
SPPPAPNGGDSSDSSDSSYENGHDNGADFDSDYGSDRSDWSLDDFARKPQSDEDDRAPLQLREAEQSLREFLMEQLAPLK
LSMRDKGLVIFLIESLDDEGYLSGTLDEILADLPPELEVELDELQAALRMLQSFDPPGIGARSAAECLSLQLHRLDSPAK
GLALIIVNQHLELLAARDYTRLKKALCVDEPTLKAAHELIRSLAPFPGHAFGRAEADFVVPDVIVRKTSAGWMAQLNPDV
MPRLRINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADT
LGLHESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVV
ARRTVAKYREALKIPAVNLRKSL
>Mature_503_residues
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSLNALQSNSATPLPTE
SPPPAPNGGDSSDSSDSSYENGHDNGADFDSDYGSDRSDWSLDDFARKPQSDEDDRAPLQLREAEQSLREFLMEQLAPLK
LSMRDKGLVIFLIESLDDEGYLSGTLDEILADLPPELEVELDELQAALRMLQSFDPPGIGARSAAECLSLQLHRLDSPAK
GLALIIVNQHLELLAARDYTRLKKALCVDEPTLKAAHELIRSLAPFPGHAFGRAEADFVVPDVIVRKTSAGWMAQLNPDV
MPRLRINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADT
LGLHESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVV
ARRTVAKYREALKIPAVNLRKSL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=506, Percent_Identity=44.8616600790514, Blast_Score=413, Evalue=1e-116,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 55740; Mature: 55740

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARI
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
GADGSLNALQSNSATPLPTESPPPAPNGGDSSDSSDSSYENGHDNGADFDSDYGSDRSDW
CCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SLDDFARKPQSDEDDRAPLQLREAEQSLREFLMEQLAPLKLSMRDKGLVIFLIESLDDEG
CHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEEECCCCCC
YLSGTLDEILADLPPELEVELDELQAALRMLQSFDPPGIGARSAAECLSLQLHRLDSPAK
CCCCCHHHHHHCCCCCCEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCC
GLALIIVNQHLELLAARDYTRLKKALCVDEPTLKAAHELIRSLAPFPGHAFGRAEADFVV
CEEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PDVIVRKTSAGWMAQLNPDVMPRLRINDMYAQILRSSRGESGAANLQQKLQEARWLIKNI
CHHHHEECCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
QQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLHESTISRVTTNKYMATP
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEECC
MGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVV
CCCEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHH
ARRTVAKYREALKIPAVNLRKSL
HHHHHHHHHHHHCCCCHHHHHCC
>Mature Secondary Structure
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARI
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
GADGSLNALQSNSATPLPTESPPPAPNGGDSSDSSDSSYENGHDNGADFDSDYGSDRSDW
CCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SLDDFARKPQSDEDDRAPLQLREAEQSLREFLMEQLAPLKLSMRDKGLVIFLIESLDDEG
CHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEEECCCCCC
YLSGTLDEILADLPPELEVELDELQAALRMLQSFDPPGIGARSAAECLSLQLHRLDSPAK
CCCCCHHHHHHCCCCCCEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCC
GLALIIVNQHLELLAARDYTRLKKALCVDEPTLKAAHELIRSLAPFPGHAFGRAEADFVV
CEEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PDVIVRKTSAGWMAQLNPDVMPRLRINDMYAQILRSSRGESGAANLQQKLQEARWLIKNI
CHHHHEECCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
QQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLHESTISRVTTNKYMATP
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEECC
MGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVV
CCCEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHH
ARRTVAKYREALKIPAVNLRKSL
HHHHHHHHHHHHCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1417413 [H]