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The map label for this gene is 187927308

Identifier: 187927308

GI number: 187927308

Start: 218677

End: 219972

Strand: Direct

Name: 187927308

Synonym: Rpic_0200

Alternate gene names: NA

Gene position: 218677-219972 (Clockwise)

Preceding gene: 187927307

Following gene: 187927309

Centisome position: 5.55

GC content: 61.96

Gene sequence:

>1296_bases
ATGGTTCCGCACTTGATCACCGCGCTGAACGGGCCGCTGCTTGAGCTCGAGCAGAAGATTCTTGACGCCACGCCCGCCAT
CGAACGCTGGTTCCGCCTGGAATGGCAGGAACACACGCCGCCGTTCTACTGTTCCGTCGATTTGCGCAACGCCGGCTTCA
AGCTGGCGCCGGTCGACACGAACCTGTTCCCGGGCGGCTTCAACAACCTCGCGCCCGAGATGCTGCCGCTGGCTGTGCAA
GCCGCCATGGCCGCCATCGAAAAAATCTGCCCCGACGCCAAGAACCTCCTGCTGATCCCCGAGCGCCACACGCGCAACAT
GTTCTATCTGCAGAACGTGGCGCGCCTGTCGCAGATCATGCGCCAAGCGGGGCTGAATGTGCGTCTCGGCTCGCTCTCCG
AAGACATCACCGAGCCGACCTCGATCGATCTGCCCGATGGACAGCGCCTAGTGGTCGAACCGATTAAGCGCATCGGTACC
AAGGGCCGTCGCTTGGGCATGGAAGACTTCGATCCGTGCTCGATCCTGCTGAACAACGATCTGTCAGCCGGCGTGCCGCC
CATCCTCGAGAACATCAACGAGCAATATCTGCTGCCGCCACTCCATGCCGGCTGGTCGCAGCGCCGCAAGACCAACCACT
TTGCCGCCTACGATGAAGTGGCCAAGAAGTTCGCCAAGCTGATCGACATCGACCCGTGGATGGTCAACCCGTACTTCACC
AAATGCAGCGGACTGGACTTCCATGAGCGCGCCGGCGAGGAGGAACTCGCCCACGCGGTCGAGACGGTGCTGAAGAAAAC
CGCCAAGAAGTACAAGGAATACGGCGTGACCGAGACCCCCTACGTGGTGGTCAAGGCCGACGCCGGCACCTATGGCATGG
GTGTGATGACCGTGCGCGATCCTTCCGAGGTCAAGGGCCTCAACCGGAAGGAGCGCAACAAGATGAGCGTGGTCAAGGAG
GGCCTGGAGGTCTCCGAAGTGATCGTGCAGGAAGGCGTGCACACCTTCGAGAAGATCAACGAAGCCGTGGCCGAGCCCGT
CGTCTACATGATCGACCGCTATGTGGTGGGCGGTTTCTACCGGGTTCACACGGGCCGCGGCGTTGACGAGAATCTCAATG
CGCCGGGCATGCATTTCGTGCCGCTGCCGTTTGCGTCGAATTCGCTGCCGGACAGCCATGCCAAGCCGGGCGCCGGTGAG
CCGAACCGCTTCTATATGTACGGCGTCGTGGCGCGCTTGGCGCTGTTGGCTGCATCGCTCGAGCTTGAGAAGACCGACCC
GAACCCGCTGATCTGA

Upstream 100 bases:

>100_bases
CTCTTTCCTGACCGCATATCGATAGCCCGCTGTCGTCTGCAAGCTATTGTCTGTTCCTCTACAATTGGAGTGGGCGATAA
ATAACATCAATAGGAAGGGT

Downstream 100 bases:

>100_bases
TCGGCCGTATCCCCGCTGCAGAAGGAACCGCCATGCGCATCCTGTTCATCGTCGATCCGCTGTCGACGTTCAAGACCTAC
AAGGATTCCACCTTCGCGAT

Product: glutamate/cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Glutamate-Cysteine Ligase GshA; Glutamate-Cysteine Ligase-Related Protein; Glutathione Synthetase; GshA Protein

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MVPHLITALNGPLLELEQKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDTNLFPGGFNNLAPEMLPLAVQ
AAMAAIEKICPDAKNLLLIPERHTRNMFYLQNVARLSQIMRQAGLNVRLGSLSEDITEPTSIDLPDGQRLVVEPIKRIGT
KGRRLGMEDFDPCSILLNNDLSAGVPPILENINEQYLLPPLHAGWSQRRKTNHFAAYDEVAKKFAKLIDIDPWMVNPYFT
KCSGLDFHERAGEEELAHAVETVLKKTAKKYKEYGVTETPYVVVKADAGTYGMGVMTVRDPSEVKGLNRKERNKMSVVKE
GLEVSEVIVQEGVHTFEKINEAVAEPVVYMIDRYVVGGFYRVHTGRGVDENLNAPGMHFVPLPFASNSLPDSHAKPGAGE
PNRFYMYGVVARLALLAASLELEKTDPNPLI

Sequences:

>Translated_431_residues
MVPHLITALNGPLLELEQKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDTNLFPGGFNNLAPEMLPLAVQ
AAMAAIEKICPDAKNLLLIPERHTRNMFYLQNVARLSQIMRQAGLNVRLGSLSEDITEPTSIDLPDGQRLVVEPIKRIGT
KGRRLGMEDFDPCSILLNNDLSAGVPPILENINEQYLLPPLHAGWSQRRKTNHFAAYDEVAKKFAKLIDIDPWMVNPYFT
KCSGLDFHERAGEEELAHAVETVLKKTAKKYKEYGVTETPYVVVKADAGTYGMGVMTVRDPSEVKGLNRKERNKMSVVKE
GLEVSEVIVQEGVHTFEKINEAVAEPVVYMIDRYVVGGFYRVHTGRGVDENLNAPGMHFVPLPFASNSLPDSHAKPGAGE
PNRFYMYGVVARLALLAASLELEKTDPNPLI
>Mature_431_residues
MVPHLITALNGPLLELEQKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDTNLFPGGFNNLAPEMLPLAVQ
AAMAAIEKICPDAKNLLLIPERHTRNMFYLQNVARLSQIMRQAGLNVRLGSLSEDITEPTSIDLPDGQRLVVEPIKRIGT
KGRRLGMEDFDPCSILLNNDLSAGVPPILENINEQYLLPPLHAGWSQRRKTNHFAAYDEVAKKFAKLIDIDPWMVNPYFT
KCSGLDFHERAGEEELAHAVETVLKKTAKKYKEYGVTETPYVVVKADAGTYGMGVMTVRDPSEVKGLNRKERNKMSVVKE
GLEVSEVIVQEGVHTFEKINEAVAEPVVYMIDRYVVGGFYRVHTGRGVDENLNAPGMHFVPLPFASNSLPDSHAKPGAGE
PNRFYMYGVVARLALLAASLELEKTDPNPLI

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 48097; Mature: 48097

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPHLITALNGPLLELEQKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDT
CCCCHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCHHCCCCCEEEEEEECCCCEEEEECCC
NLFPGGFNNLAPEMLPLAVQAAMAAIEKICPDAKNLLLIPERHTRNMFYLQNVARLSQIM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHH
RQAGLNVRLGSLSEDITEPTSIDLPDGQRLVVEPIKRIGTKGRRLGMEDFDPCSILLNND
HHCCCCEEECCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCC
LSAGVPPILENINEQYLLPPLHAGWSQRRKTNHFAAYDEVAKKFAKLIDIDPWMVNPYFT
CCCCCCHHHHCCCCCEECCCHHCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHH
KCSGLDFHERAGEEELAHAVETVLKKTAKKYKEYGVTETPYVVVKADAGTYGMGVMTVRD
CCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECC
PSEVKGLNRKERNKMSVVKEGLEVSEVIVQEGVHTFEKINEAVAEPVVYMIDRYVVGGFY
HHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
RVHTGRGVDENLNAPGMHFVPLPFASNSLPDSHAKPGAGEPNRFYMYGVVARLALLAASL
EEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ELEKTDPNPLI
EEECCCCCCCC
>Mature Secondary Structure
MVPHLITALNGPLLELEQKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDT
CCCCHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCHHCCCCCEEEEEEECCCCEEEEECCC
NLFPGGFNNLAPEMLPLAVQAAMAAIEKICPDAKNLLLIPERHTRNMFYLQNVARLSQIM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHH
RQAGLNVRLGSLSEDITEPTSIDLPDGQRLVVEPIKRIGTKGRRLGMEDFDPCSILLNND
HHCCCCEEECCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCC
LSAGVPPILENINEQYLLPPLHAGWSQRRKTNHFAAYDEVAKKFAKLIDIDPWMVNPYFT
CCCCCCHHHHCCCCCEECCCHHCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHH
KCSGLDFHERAGEEELAHAVETVLKKTAKKYKEYGVTETPYVVVKADAGTYGMGVMTVRD
CCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECC
PSEVKGLNRKERNKMSVVKEGLEVSEVIVQEGVHTFEKINEAVAEPVVYMIDRYVVGGFY
HHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
RVHTGRGVDENLNAPGMHFVPLPFASNSLPDSHAKPGAGEPNRFYMYGVVARLALLAASL
EEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ELEKTDPNPLI
EEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA