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The map label for this gene is amtB [H]

Identifier: 187927307

GI number: 187927307

Start: 216763

End: 218310

Strand: Direct

Name: amtB [H]

Synonym: Rpic_0199

Alternate gene names: 187927307

Gene position: 216763-218310 (Clockwise)

Preceding gene: 187927306

Following gene: 187927308

Centisome position: 5.5

GC content: 66.54

Gene sequence:

>1548_bases
ATGAAAACCTGGTTCACACGATTCCTGACGGCTGGGGCCGTCGCTGCCGTGCTCGCAGGCGCCGTGATCTCGGCGCCGGC
CGCCGCACAGGACAAGCCGGCGGCTGAAGCTTCGGCGCCGGTTGCCGCGGCCGCCGCTTCCGAGCCGGCCGCTGCCGCAC
CGGCAGCCGCAGCTCCGGCTGCAGCCCCCGCCGCCGCAGCTGCTCCAGCTGCCGCTGCGCCCGCCGCTGCTGCGCCCGTC
CCCAACAAGGGCGACACGGCGTGGCTGCTGGTCTCCACCGCCTTCGTCATCTTGATGACGCTGCCGGGCCTGGCGCTGTT
CTATGGCGGCCTGGTGCGCAAGAAGAACATGCTGTCGGTGCTGATGCAGTGCATGGGCATCTTCTCGCTCATCATCATCC
TGTGGGCCATCTACGGCTACAGCTTTGCGTTTACCGAGGGCAACGCGTTCTTCGGCGGGCTGGATCGCCTGTTCCTGAAG
GGTCTGACGCCCGATTCGGTTGCCGCCACCTTCAGCAAAGGCGTGGTGGTGCCGGAATACGCGTACTTCGCCTTCCAAGG
CGCGTTTGCGGCGATTACCTGCGCTCTGATCATCGGTGCTTTCGCCGAGCGTGCGAAGTTCTCCGCCGTGCTGCTGTTCG
TGGTGCTGTGGTTCACCTTCAGCTACGTGCCGATCGCCCACATGGTCTGGTTCTGGCCGGGTCCGGACGGCTTTACCGAC
GCCAAGGCGGCCGAGGTGATGACGGCCAAGTCGGGTTGGCTGTTCCAGAAGGGCGCGCTGGACTTTGCCGGTGGCACCGT
GGTGCACATCAACGCTGCTGTGGCGGGTCTGGTGGGCGCTTTCCTGTTCGGCAAGCGCATCGGCTTTGGCCGTGAGGCGC
TCAAGCCGCACAGCCTGACGCTGACGATGGTCGGTGCCTCGCTGCTCTGGTTCGGCTGGTTCGGCTTCAACGCCGGCTCG
GCCCTGGAAGCCAACGGCAGCGCCGCGCTGGCCTTCGTCAACACGCTGCTGGCGACCGCCGCTGCCGTGCTGTCGTGGAG
CTTCGGCGAGTGGTTCGGCAAGAGCAAGCCTTCGATGCTGGGTGCCGCATCGGGTGCCGTGGCTGGCCTGGTGGCCATCA
CCCCGGCCGCCGGCTTTGTCGGCCCGATGGGTTCGATCGCGCTCGGCATCATCGCCGGCCTGCTGTGCCTGTGGGGCGTG
AATGGCCTCAAGCGCATGCTGGGCATGGACGACACGCTGGACGTGTTCGGTGTGCACGGCGTGGGCGGTATCCTCGGCGC
GCTGCTGACCGGCGTCTTCGCGGCGCCGAGCCTGGGCGGCACCGGCATCTACGACTATGTCGCCAACAAGGTAGCCGACG
ATTACTCGATCGGCGGCCAGCTGTGGATCCAGTTCCAGGGCGTGGCGACCACGGTCATCTGGTCTGCAGTGGTGGCGTTC
ATTTCGTACAAGATCGTCGATGCCATTGTTGGCCTGCGCGTGCCGGAAGAAGAGGAGCGCGAGGGCCTGGACATCACGTC
CCACGGCGAAACTGCCTACGAAGACTGA

Upstream 100 bases:

>100_bases
GATCTTCGTGGCCGACGTCGAACAAGTCATCCGCATCCGCACCGGTGAGACCGGTGGCGACGCCCTATAAGCCCCAATCC
GCCAAGGAAGAGGTCAAAAC

Downstream 100 bases:

>100_bases
TCGTCTGCGTCATCCCGCAAAAGACCCGGCCGCGCGCCGGGTTTTTTGTGTCGCCACCTGAGCCGGACGTCCTTAAGTCC
GATGTAAAAAAGCTTCAATT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MKTWFTRFLTAGAVAAVLAGAVISAPAAAQDKPAAEASAPVAAAAASEPAAAAPAAAAPAAAPAAAAAPAAAAPAAAAPV
PNKGDTAWLLVSTAFVILMTLPGLALFYGGLVRKKNMLSVLMQCMGIFSLIIILWAIYGYSFAFTEGNAFFGGLDRLFLK
GLTPDSVAATFSKGVVVPEYAYFAFQGAFAAITCALIIGAFAERAKFSAVLLFVVLWFTFSYVPIAHMVWFWPGPDGFTD
AKAAEVMTAKSGWLFQKGALDFAGGTVVHINAAVAGLVGAFLFGKRIGFGREALKPHSLTLTMVGASLLWFGWFGFNAGS
ALEANGSAALAFVNTLLATAAAVLSWSFGEWFGKSKPSMLGAASGAVAGLVAITPAAGFVGPMGSIALGIIAGLLCLWGV
NGLKRMLGMDDTLDVFGVHGVGGILGALLTGVFAAPSLGGTGIYDYVANKVADDYSIGGQLWIQFQGVATTVIWSAVVAF
ISYKIVDAIVGLRVPEEEEREGLDITSHGETAYED

Sequences:

>Translated_515_residues
MKTWFTRFLTAGAVAAVLAGAVISAPAAAQDKPAAEASAPVAAAAASEPAAAAPAAAAPAAAPAAAAAPAAAAPAAAAPV
PNKGDTAWLLVSTAFVILMTLPGLALFYGGLVRKKNMLSVLMQCMGIFSLIIILWAIYGYSFAFTEGNAFFGGLDRLFLK
GLTPDSVAATFSKGVVVPEYAYFAFQGAFAAITCALIIGAFAERAKFSAVLLFVVLWFTFSYVPIAHMVWFWPGPDGFTD
AKAAEVMTAKSGWLFQKGALDFAGGTVVHINAAVAGLVGAFLFGKRIGFGREALKPHSLTLTMVGASLLWFGWFGFNAGS
ALEANGSAALAFVNTLLATAAAVLSWSFGEWFGKSKPSMLGAASGAVAGLVAITPAAGFVGPMGSIALGIIAGLLCLWGV
NGLKRMLGMDDTLDVFGVHGVGGILGALLTGVFAAPSLGGTGIYDYVANKVADDYSIGGQLWIQFQGVATTVIWSAVVAF
ISYKIVDAIVGLRVPEEEEREGLDITSHGETAYED
>Mature_515_residues
MKTWFTRFLTAGAVAAVLAGAVISAPAAAQDKPAAEASAPVAAAAASEPAAAAPAAAAPAAAPAAAAAPAAAAPAAAAPV
PNKGDTAWLLVSTAFVILMTLPGLALFYGGLVRKKNMLSVLMQCMGIFSLIIILWAIYGYSFAFTEGNAFFGGLDRLFLK
GLTPDSVAATFSKGVVVPEYAYFAFQGAFAAITCALIIGAFAERAKFSAVLLFVVLWFTFSYVPIAHMVWFWPGPDGFTD
AKAAEVMTAKSGWLFQKGALDFAGGTVVHINAAVAGLVGAFLFGKRIGFGREALKPHSLTLTMVGASLLWFGWFGFNAGS
ALEANGSAALAFVNTLLATAAAVLSWSFGEWFGKSKPSMLGAASGAVAGLVAITPAAGFVGPMGSIALGIIAGLLCLWGV
NGLKRMLGMDDTLDVFGVHGVGGILGALLTGVFAAPSLGGTGIYDYVANKVADDYSIGGQLWIQFQGVATTVIWSAVVAF
ISYKIVDAIVGLRVPEEEEREGLDITSHGETAYED

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=439, Percent_Identity=55.5808656036446, Blast_Score=481, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17550182, Length=456, Percent_Identity=29.1666666666667, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17550180, Length=451, Percent_Identity=27.7161862527716, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI32564042, Length=448, Percent_Identity=26.5625, Blast_Score=119, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI133931100, Length=445, Percent_Identity=25.3932584269663, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324187, Length=453, Percent_Identity=36.4238410596026, Blast_Score=233, Evalue=5e-62,
Organism=Saccharomyces cerevisiae, GI6325396, Length=434, Percent_Identity=32.7188940092166, Blast_Score=213, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6321559, Length=434, Percent_Identity=31.7972350230415, Blast_Score=207, Evalue=2e-54,
Organism=Drosophila melanogaster, GI161078314, Length=441, Percent_Identity=26.7573696145125, Blast_Score=108, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 52856; Mature: 52856

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTWFTRFLTAGAVAAVLAGAVISAPAAAQDKPAAEASAPVAAAAASEPAAAAPAAAAPA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCC
AAPAAAAAPAAAAPAAAAPVPNKGDTAWLLVSTAFVILMTLPGLALFYGGLVRKKNMLSV
CCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMQCMGIFSLIIILWAIYGYSFAFTEGNAFFGGLDRLFLKGLTPDSVAATFSKGVVVPEY
HHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCH
AYFAFQGAFAAITCALIIGAFAERAKFSAVLLFVVLWFTFSYVPIAHMVWFWPGPDGFTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCC
AKAAEVMTAKSGWLFQKGALDFAGGTVVHINAAVAGLVGAFLFGKRIGFGREALKPHSLT
HHHHHHHCCCCCCEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCHHHCCCCCEE
LTMVGASLLWFGWFGFNAGSALEANGSAALAFVNTLLATAAAVLSWSFGEWFGKSKPSML
EHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
GAASGAVAGLVAITPAAGFVGPMGSIALGIIAGLLCLWGVNGLKRMLGMDDTLDVFGVHG
HCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHCCCH
VGGILGALLTGVFAAPSLGGTGIYDYVANKVADDYSIGGQLWIQFQGVATTVIWSAVVAF
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHH
ISYKIVDAIVGLRVPEEEEREGLDITSHGETAYED
HHHHHHHHHHCCCCCCHHHHCCCCEECCCCCCCCC
>Mature Secondary Structure
MKTWFTRFLTAGAVAAVLAGAVISAPAAAQDKPAAEASAPVAAAAASEPAAAAPAAAAPA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCC
AAPAAAAAPAAAAPAAAAPVPNKGDTAWLLVSTAFVILMTLPGLALFYGGLVRKKNMLSV
CCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMQCMGIFSLIIILWAIYGYSFAFTEGNAFFGGLDRLFLKGLTPDSVAATFSKGVVVPEY
HHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCH
AYFAFQGAFAAITCALIIGAFAERAKFSAVLLFVVLWFTFSYVPIAHMVWFWPGPDGFTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCC
AKAAEVMTAKSGWLFQKGALDFAGGTVVHINAAVAGLVGAFLFGKRIGFGREALKPHSLT
HHHHHHHCCCCCCEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCHHHCCCCCEE
LTMVGASLLWFGWFGFNAGSALEANGSAALAFVNTLLATAAAVLSWSFGEWFGKSKPSML
EHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
GAASGAVAGLVAITPAAGFVGPMGSIALGIIAGLLCLWGVNGLKRMLGMDDTLDVFGVHG
HCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHCCCH
VGGILGALLTGVFAAPSLGGTGIYDYVANKVADDYSIGGQLWIQFQGVATTVIWSAVVAF
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHH
ISYKIVDAIVGLRVPEEEEREGLDITSHGETAYED
HHHHHHHHHHCCCCCCHHHHCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]