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The map label for this gene is lipA

Identifier: 187927286

GI number: 187927286

Start: 197460

End: 198461

Strand: Reverse

Name: lipA

Synonym: Rpic_0178

Alternate gene names: 187927286

Gene position: 198461-197460 (Counterclockwise)

Preceding gene: 187927287

Following gene: 187927284

Centisome position: 5.03

GC content: 64.27

Gene sequence:

>1002_bases
ATGACCGATTCCGCCGCCGGCGCCACTGAAGTCGCCACCCCTGCCACCCCGTCGAACAAACCGTACGACGCGACGGCCAA
GCAGAAGTCGCTCGACAAAACCGCACGCATCCCCATCAAGATCGTGCCGGCCGAGAAGCTCAAGAAGCCGGATTGGATTC
GCGTGCGCGCCGCCACCGGCAACTCGCGCTTCTACGAAATCAAAGACATCCTGCGCGCCAACAACCTCGTGACGGTGTGC
GAAGAGGCCAGCTGCCCGAATATCGGCGAGTGCTTCGGCAAGGGCACGGCCACGTTCATGATCATGGGCGACAAATGCAC
GCGCCGCTGCCCGTTCTGCGATGTCGGGCACGGTCGCCCGGATCCGCTCGACGTGAACGAGCCGGAAAACCTGGCCAAGA
CGATCGCTGAACTCAAGCTCAACTACGTCGTCATCACCAGCGTCGACCGCGACGACCTGCGTGACGGCGGCGCCCAGCAT
TACGTCGATTGCATCTCGCGCACGCGCGCCCTGTCGCCGGCCACGCGCATCGAAGTGCTGGTGCCGGACTTCCGCGGCCG
CTTGGAGAAGGCGCTGGACATCCTGCAGGAATGCCCGCCCGACGTGATGAACCACAACCTCGAAACGGTGCCGCGCCTGT
ACAAGCAGGCGCGCCCGGGTGCGGATTACGCGCACTCGCTGAAGCTGCTGAAGGACTTCAAGGCCCGCAACCCGAACGTG
CCGACCAAGTCCGGCCTGATGGTCGGCCTGGGCGAGACGGATGAGGAAATCCTGGAAGTCATGCGCGACATGCGCGAGCA
CGACATCGACATGCTGACCATCGGCCAGTATCTGGCGCCGTCGGGCCACCATCTGCCGGTGCTGCGCTATGTGCACCCGG
ACACCTTCAAGATGTTCGAAGAGAAGGCGTACGAGATGGGCTTCACGCACGCGGCCGTGGGCGCCATGGTGCGCAGTTCG
TACCACGCGGATCAGCAGGCACACGAAGCCGGCGTGGTCTGA

Upstream 100 bases:

>100_bases
GGTCGCTCAATTGCAGCAACGCGCGCAAGCGCACCCCGCCGAGGCCGCTACCGCATAGCGCCCGGATATGCACGAGCCGC
AAGCCCGTGCGGAGAACACG

Downstream 100 bases:

>100_bases
GTACCGCCGGAACGCTGCAGAAACAAAAAGGGCCTTGGGGTAGAACCCAAGGCCCTTTTGCTTTGGTACGGCTCGATCAG
GCTTGCGAAGCGGCGTCCTT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MTDSAAGATEVATPATPSNKPYDATAKQKSLDKTARIPIKIVPAEKLKKPDWIRVRAATGNSRFYEIKDILRANNLVTVC
EEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDVNEPENLAKTIAELKLNYVVITSVDRDDLRDGGAQH
YVDCISRTRALSPATRIEVLVPDFRGRLEKALDILQECPPDVMNHNLETVPRLYKQARPGADYAHSLKLLKDFKARNPNV
PTKSGLMVGLGETDEEILEVMRDMREHDIDMLTIGQYLAPSGHHLPVLRYVHPDTFKMFEEKAYEMGFTHAAVGAMVRSS
YHADQQAHEAGVV

Sequences:

>Translated_333_residues
MTDSAAGATEVATPATPSNKPYDATAKQKSLDKTARIPIKIVPAEKLKKPDWIRVRAATGNSRFYEIKDILRANNLVTVC
EEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDVNEPENLAKTIAELKLNYVVITSVDRDDLRDGGAQH
YVDCISRTRALSPATRIEVLVPDFRGRLEKALDILQECPPDVMNHNLETVPRLYKQARPGADYAHSLKLLKDFKARNPNV
PTKSGLMVGLGETDEEILEVMRDMREHDIDMLTIGQYLAPSGHHLPVLRYVHPDTFKMFEEKAYEMGFTHAAVGAMVRSS
YHADQQAHEAGVV
>Mature_332_residues
TDSAAGATEVATPATPSNKPYDATAKQKSLDKTARIPIKIVPAEKLKKPDWIRVRAATGNSRFYEIKDILRANNLVTVCE
EASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDVNEPENLAKTIAELKLNYVVITSVDRDDLRDGGAQHY
VDCISRTRALSPATRIEVLVPDFRGRLEKALDILQECPPDVMNHNLETVPRLYKQARPGADYAHSLKLLKDFKARNPNVP
TKSGLMVGLGETDEEILEVMRDMREHDIDMLTIGQYLAPSGHHLPVLRYVHPDTFKMFEEKAYEMGFTHAAVGAMVRSSY
HADQQAHEAGVV

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=294, Percent_Identity=46.9387755102041, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI37577164, Length=256, Percent_Identity=47.65625, Blast_Score=231, Evalue=7e-61,
Organism=Escherichia coli, GI1786846, Length=304, Percent_Identity=63.4868421052632, Blast_Score=408, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI32564533, Length=255, Percent_Identity=43.5294117647059, Blast_Score=215, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6324770, Length=291, Percent_Identity=45.360824742268, Blast_Score=251, Evalue=8e-68,
Organism=Drosophila melanogaster, GI221513272, Length=288, Percent_Identity=47.2222222222222, Blast_Score=264, Evalue=5e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_RALPJ (B2UE01)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001897773.1
- GeneID:   6289347
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_0178
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 36989; Mature: 36857

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSAAGATEVATPATPSNKPYDATAKQKSLDKTARIPIKIVPAEKLKKPDWIRVRAATG
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECHHHCCCCCEEEEEEECC
NSRFYEIKDILRANNLVTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRP
CCCHHHHHHHHHHCCEEEEECCCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCCC
DPLDVNEPENLAKTIAELKLNYVVITSVDRDDLRDGGAQHYVDCISRTRALSPATRIEVL
CCCCCCCHHHHHHHHHHHHCCEEEEEECCHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEE
VPDFRGRLEKALDILQECPPDVMNHNLETVPRLYKQARPGADYAHSLKLLKDFKARNPNV
CCHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
PTKSGLMVGLGETDEEILEVMRDMREHDIDMLTIGQYLAPSGHHLPVLRYVHPDTFKMFE
CCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCEEEEECCHHHHHHH
EKAYEMGFTHAAVGAMVRSSYHADQQAHEAGVV
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TDSAAGATEVATPATPSNKPYDATAKQKSLDKTARIPIKIVPAEKLKKPDWIRVRAATG
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECHHHCCCCCEEEEEEECC
NSRFYEIKDILRANNLVTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRP
CCCHHHHHHHHHHCCEEEEECCCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCCC
DPLDVNEPENLAKTIAELKLNYVVITSVDRDDLRDGGAQHYVDCISRTRALSPATRIEVL
CCCCCCCHHHHHHHHHHHHCCEEEEEECCHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEE
VPDFRGRLEKALDILQECPPDVMNHNLETVPRLYKQARPGADYAHSLKLLKDFKARNPNV
CCHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
PTKSGLMVGLGETDEEILEVMRDMREHDIDMLTIGQYLAPSGHHLPVLRYVHPDTFKMFE
CCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCEEEEECCHHHHHHH
EKAYEMGFTHAAVGAMVRSSYHADQQAHEAGVV
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA