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The map label for this gene is paaF [C]

Identifier: 187927267

GI number: 187927267

Start: 177254

End: 178072

Strand: Direct

Name: paaF [C]

Synonym: Rpic_0159

Alternate gene names: 187927267

Gene position: 177254-178072 (Clockwise)

Preceding gene: 187927266

Following gene: 187927268

Centisome position: 4.5

GC content: 66.54

Gene sequence:

>819_bases
ATGACCTCGCTCGCCCCCCGCTTTGCCCGCTACCAGGCGCTGACGCTCATCCAGCACGGCCCCATCCTCGAAATCGTGAT
GGGCGCGGCGCAGTCGGCCAACCGCAAGCTGTCCACGGCCGATGCCGGCCTGCACCGCGAACTGGCGGAAATCTGGCGCG
ATGTGTCCGCCGAGCCCTCCGTGCGCGTCGCGCTCATCCGCGGTGAGGGCAAGGGCTTCTCGGCTGGCGGCGATCTGCAC
CTCGTCGAGCAGATGGCTGATGACTTCGACGTGCGCACGCGCGTCTGGCACGAGGCGCGCGACCTTGTCTACAACGTCAT
CAACTGCGACAAGCCGATTGTCAGCGCCATGCACGGCCCGGCCGTGGGGGCAGGGCTCGTTGCCGGATTGCTGGCCGATA
TTTCGATCGCCGCGAAGGACGCCCGCATCGTCGACGGCCACACGCGCCTGGGCGTGGCGGCGGGCGACCATGCGGCCATT
GTCTGGCCGTTGTTGTGCGGTATGGCCAAGGCCAAGTATTACCTGCTGCTGTGCGAGTCCATGACCGGCGAAGAGGCCGA
GCGCATTGGGCTGATTTCGCTGGCCGTGCCGGAATCGGAACTCGTCAACCGCGCATTCGAGGTCGCCGAGCGCTTGGCGG
CCGGTTCGCAGACGGCCATTCGCTGGACCAAGTACGCGCTGAACAACTGGTTGCGCCTGGCGGGCCCGAGTTTTGATACG
TCGCTGGCGCTTGAATTCATGGGTTTTGCGGGCCCTGACGTGCGCGAGGGCATTGCGAGCCTGCGCCAGAAGCGTCCGCC
CACGTTTGATGGCGCCTGA

Upstream 100 bases:

>100_bases
TGGCCGATGCCGCGAAGTGAAAGTGACGATGACGACGGAGCGCATCCCGCATTGCACGCGCGATCTCATCCGCTATGCTT
GAACCATTACCTTGGACGCC

Downstream 100 bases:

>100_bases
TTGAAGATGAAGACACGCATGCGCGCCCGCCCCCCGACCGATTTGTAGGAGACACCATGTTCACCCAGATGCCCAATTTT
GCCGAAGGCGGTTTCGATTT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MTSLAPRFARYQALTLIQHGPILEIVMGAAQSANRKLSTADAGLHRELAEIWRDVSAEPSVRVALIRGEGKGFSAGGDLH
LVEQMADDFDVRTRVWHEARDLVYNVINCDKPIVSAMHGPAVGAGLVAGLLADISIAAKDARIVDGHTRLGVAAGDHAAI
VWPLLCGMAKAKYYLLLCESMTGEEAERIGLISLAVPESELVNRAFEVAERLAAGSQTAIRWTKYALNNWLRLAGPSFDT
SLALEFMGFAGPDVREGIASLRQKRPPTFDGA

Sequences:

>Translated_272_residues
MTSLAPRFARYQALTLIQHGPILEIVMGAAQSANRKLSTADAGLHRELAEIWRDVSAEPSVRVALIRGEGKGFSAGGDLH
LVEQMADDFDVRTRVWHEARDLVYNVINCDKPIVSAMHGPAVGAGLVAGLLADISIAAKDARIVDGHTRLGVAAGDHAAI
VWPLLCGMAKAKYYLLLCESMTGEEAERIGLISLAVPESELVNRAFEVAERLAAGSQTAIRWTKYALNNWLRLAGPSFDT
SLALEFMGFAGPDVREGIASLRQKRPPTFDGA
>Mature_271_residues
TSLAPRFARYQALTLIQHGPILEIVMGAAQSANRKLSTADAGLHRELAEIWRDVSAEPSVRVALIRGEGKGFSAGGDLHL
VEQMADDFDVRTRVWHEARDLVYNVINCDKPIVSAMHGPAVGAGLVAGLLADISIAAKDARIVDGHTRLGVAAGDHAAIV
WPLLCGMAKAKYYLLLCESMTGEEAERIGLISLAVPESELVNRAFEVAERLAAGSQTAIRWTKYALNNWLRLAGPSFDTS
LALEFMGFAGPDVREGIASLRQKRPPTFDGA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=230, Percent_Identity=23.9130434782609, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI20127408, Length=213, Percent_Identity=27.6995305164319, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI4502327, Length=226, Percent_Identity=28.3185840707965, Blast_Score=65, Evalue=1e-10,
Organism=Escherichia coli, GI1787659, Length=225, Percent_Identity=30.6666666666667, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787660, Length=252, Percent_Identity=30.1587301587302, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI221142681, Length=211, Percent_Identity=29.8578199052133, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788597, Length=233, Percent_Identity=26.1802575107296, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17534483, Length=231, Percent_Identity=26.4069264069264, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=230, Percent_Identity=28.695652173913, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17554946, Length=242, Percent_Identity=25.6198347107438, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=222, Percent_Identity=23.8738738738739, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17540714, Length=183, Percent_Identity=24.5901639344262, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17560910, Length=203, Percent_Identity=24.1379310344828, Blast_Score=64, Evalue=1e-10,
Organism=Drosophila melanogaster, GI20129971, Length=240, Percent_Identity=25.4166666666667, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24653477, Length=240, Percent_Identity=25.4166666666667, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24653139, Length=238, Percent_Identity=25.2100840336134, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24654903, Length=193, Percent_Identity=25.3886010362694, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19922422, Length=210, Percent_Identity=24.7619047619048, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29299; Mature: 29168

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLAPRFARYQALTLIQHGPILEIVMGAAQSANRKLSTADAGLHRELAEIWRDVSAEPS
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCC
VRVALIRGEGKGFSAGGDLHLVEQMADDFDVRTRVWHEARDLVYNVINCDKPIVSAMHGP
EEEEEEECCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC
AVGAGLVAGLLADISIAAKDARIVDGHTRLGVAAGDHAAIVWPLLCGMAKAKYYLLLCES
CHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHHHEEEEEECC
MTGEEAERIGLISLAVPESELVNRAFEVAERLAAGSQTAIRWTKYALNNWLRLAGPSFDT
CCCCHHHHEEEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCH
SLALEFMGFAGPDVREGIASLRQKRPPTFDGA
HHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TSLAPRFARYQALTLIQHGPILEIVMGAAQSANRKLSTADAGLHRELAEIWRDVSAEPS
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCC
VRVALIRGEGKGFSAGGDLHLVEQMADDFDVRTRVWHEARDLVYNVINCDKPIVSAMHGP
EEEEEEECCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC
AVGAGLVAGLLADISIAAKDARIVDGHTRLGVAAGDHAAIVWPLLCGMAKAKYYLLLCES
CHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHHHEEEEEECC
MTGEEAERIGLISLAVPESELVNRAFEVAERLAAGSQTAIRWTKYALNNWLRLAGPSFDT
CCCCHHHHEEEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCH
SLALEFMGFAGPDVREGIASLRQKRPPTFDGA
HHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA