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The map label for this gene is pcaD [H]

Identifier: 187927260

GI number: 187927260

Start: 170010

End: 170849

Strand: Reverse

Name: pcaD [H]

Synonym: Rpic_0152

Alternate gene names: 187927260

Gene position: 170849-170010 (Counterclockwise)

Preceding gene: 187927261

Following gene: 187927259

Centisome position: 4.33

GC content: 66.31

Gene sequence:

>840_bases
ATGGATACCAACGCCCTCTCCGATTCCGGCACGGTGCACGCCAACCACATCGACATCCACCACCGCACCGAGGGCGCCGA
TGGCCCGTGGGTCGTGCTTGCGCACGCCTTGGGCGTAGACCACCAATTGTGGGACAGCGCCGCCCGACGCCTGGCCAGCC
GCTATCGCGTGCTGCGCTACGACGCACGTGGCCACGGCAAGACCAGCGCCCCGCACGGCCCCTACACGCTGTTCCAGATG
GCGGACGATGTGGCCGGCCTGCTCGATGCGCTATCGATCGGACAGGTGCATTTCGTTGGCCTGTCGATGGGCGGCATGGT
CGGGCAGGTCCTGGGCGTGCGGCACGCGCAGCGGCTGCTCTCGCTGACGCTGTGCGACACCGTGTGCTACACGCCGGCGT
CAGCGCATGTCATGTGGGACGAGCGCATCGGTCAAGTCGAAGCGCACGGTATGAGCGGCATTGTCGAGCCCACGATACAG
CGCTGGCTGACCACGCCGTTTCGTGAAGCCCATCCCGACGTCGTTGAACGCATCCGCGGCTTGCTGCGCGCCACACAACC
GCACGGCTATGTCGGTACATGCCTGGCCATCAAGGCGCTCGACACACGCAGCGCGCTGGCGCGCATCGCCTGTCCCACGC
TCGTCATGACAGGCGACCAAGACACCGGTGCCCCGGTGGAAGCGGCACGCGAGATCGCCACGCACATTCCTGACGCCCGT
TTGAAAGTGATTCCGCACGCGGCGCACCTGGCCCCCATCGAACAGGAGGAGGGCTTCCTCGCCGATCTGGATGAGTTCCT
CGGGCACGCAGGATGCGGGAGTCAATGCGACACGCCATAA

Upstream 100 bases:

>100_bases
CTGTCCCCCGATCGCGGCTGGCTGCCGGTACGCCTGCGCCAGACCGAGCCCAACGGCATGCAGTTCGATCTGGTCTACCA
GTCCCAGGAAGGAGGCTGAC

Downstream 100 bases:

>100_bases
GGCCTGCCTGCCTTGCCGATAGTTGCACGCGCCCCGCACACGGCCCGTCGTCTACTGGCCGAATGGCGGAAGGCCGCTTG
CTTCTTTTGATGCAGAAACG

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: 3-oxoadipate enol-lactonase I; Beta-ketoadipate enol-lactone hydrolase I; Enol-lactone hydrolase I [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MDTNALSDSGTVHANHIDIHHRTEGADGPWVVLAHALGVDHQLWDSAARRLASRYRVLRYDARGHGKTSAPHGPYTLFQM
ADDVAGLLDALSIGQVHFVGLSMGGMVGQVLGVRHAQRLLSLTLCDTVCYTPASAHVMWDERIGQVEAHGMSGIVEPTIQ
RWLTTPFREAHPDVVERIRGLLRATQPHGYVGTCLAIKALDTRSALARIACPTLVMTGDQDTGAPVEAAREIATHIPDAR
LKVIPHAAHLAPIEQEEGFLADLDEFLGHAGCGSQCDTP

Sequences:

>Translated_279_residues
MDTNALSDSGTVHANHIDIHHRTEGADGPWVVLAHALGVDHQLWDSAARRLASRYRVLRYDARGHGKTSAPHGPYTLFQM
ADDVAGLLDALSIGQVHFVGLSMGGMVGQVLGVRHAQRLLSLTLCDTVCYTPASAHVMWDERIGQVEAHGMSGIVEPTIQ
RWLTTPFREAHPDVVERIRGLLRATQPHGYVGTCLAIKALDTRSALARIACPTLVMTGDQDTGAPVEAAREIATHIPDAR
LKVIPHAAHLAPIEQEEGFLADLDEFLGHAGCGSQCDTP
>Mature_279_residues
MDTNALSDSGTVHANHIDIHHRTEGADGPWVVLAHALGVDHQLWDSAARRLASRYRVLRYDARGHGKTSAPHGPYTLFQM
ADDVAGLLDALSIGQVHFVGLSMGGMVGQVLGVRHAQRLLSLTLCDTVCYTPASAHVMWDERIGQVEAHGMSGIVEPTIQ
RWLTTPFREAHPDVVERIRGLLRATQPHGYVGTCLAIKALDTRSALARIACPTLVMTGDQDTGAPVEAAREIATHIPDAR
LKVIPHAAHLAPIEQEEGFLADLDEFLGHAGCGSQCDTP

Specific function: Not Clear. Seems To Be Implicated In The Early Steps Of Biotin Biosynthesis. [C]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR012790 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.24 [H]

Molecular weight: Translated: 30109; Mature: 30109

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTNALSDSGTVHANHIDIHHRTEGADGPWVVLAHALGVDHQLWDSAARRLASRYRVLRY
CCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHEEE
DARGHGKTSAPHGPYTLFQMADDVAGLLDALSIGQVHFVGLSMGGMVGQVLGVRHAQRLL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHH
SLTLCDTVCYTPASAHVMWDERIGQVEAHGMSGIVEPTIQRWLTTPFREAHPDVVERIRG
HHHHHHHHHCCCCCCEEEEHHHCCCCHHCCCCCCHHHHHHHHHCCCHHHCCHHHHHHHHH
LLRATQPHGYVGTCLAIKALDTRSALARIACPTLVMTGDQDTGAPVEAAREIATHIPDAR
HHHCCCCCCCEEHHHHEEHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHCCCCC
LKVIPHAAHLAPIEQEEGFLADLDEFLGHAGCGSQCDTP
EEEECCHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MDTNALSDSGTVHANHIDIHHRTEGADGPWVVLAHALGVDHQLWDSAARRLASRYRVLRY
CCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHEEE
DARGHGKTSAPHGPYTLFQMADDVAGLLDALSIGQVHFVGLSMGGMVGQVLGVRHAQRLL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHH
SLTLCDTVCYTPASAHVMWDERIGQVEAHGMSGIVEPTIQRWLTTPFREAHPDVVERIRG
HHHHHHHHHCCCCCCEEEEHHHCCCCHHCCCCCCHHHHHHHHHCCCHHHCCHHHHHHHHH
LLRATQPHGYVGTCLAIKALDTRSALARIACPTLVMTGDQDTGAPVEAAREIATHIPDAR
HHHCCCCCCCEEHHHHEEHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHCCCCC
LKVIPHAAHLAPIEQEEGFLADLDEFLGHAGCGSQCDTP
EEEECCHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8181753 [H]