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The map label for this gene is metH [H]

Identifier: 187927257

GI number: 187927257

Start: 167735

End: 168775

Strand: Reverse

Name: metH [H]

Synonym: Rpic_0149

Alternate gene names: 187927257

Gene position: 168775-167735 (Counterclockwise)

Preceding gene: 187927259

Following gene: 187927256

Centisome position: 4.28

GC content: 65.13

Gene sequence:

>1041_bases
ATGACCGCGCCCCTGCCCTACACCCGTGCCGCCAACCTGCCCGCGCTGCTGCGCGAGCGCATCCTGATCCTGGACGGCGC
GATGGGCACCATGATCCAGCGCTACAAGCTGAATGAGGCGCAATACCGCGGCGAGCGCTTTGCCAACCACCCGATCGATG
TGAAGGGCAACAACGAGCTGCTGCTGCTCACCCGCCCCGACGTCATCCGTGAAATCCATGAGCAGTACCTAGCTGCCGGC
GCGGACCTGATCGAAACCAACACCTTCGGTGCGACGACCATCGCTCAGGAAGACTACAAGATGGCGGAGCTGGCCTACGA
GATGAACGTCGTGGCCGCCCGCCTGGCACGCGAAGCCTGCGACAAATACAGCACGCCCGACAAGCCGCGCTTTGTTGCCG
GCGCCTTTGGGCCGACGCCCAAGACCGCCAGCATTTCGCCCGACGTGAACGACCCGGGCGCTCGCAACGTCAGCTTCGAC
CAACTGCGCGACGCCTACTACGAGCAGGGCAAGGCGCTGCTCGAAGGCGGCGCGGACGTGTTTCTGGTCGAGACCATCTT
CGACACGCTCAACGCCAAGGCAGCGCTGTTCGCCATCGATCAGCTGTTTGAAGACACCGGCGAGCGCGTGCCCGTGATGA
TCTCCGGCACCGTCACCGACGCCTCGGGCCGCATTCTCTCGGGCCAAACCGTCGAGGCGTTCTGGAACAGCCTGCGCCAC
GCCAAGCCGATCACCTTTGGCCTGAACTGCGCGCTGGGCGCCGCGTTGATGCGTCCGTACATCGCCGAGCTGGCGAAGAT
TTGCGATACGGCCGTCTCGTGCTACCCCAACGCCGGCCTGCCCAACCCAATGAGCGAGACGGGCTTTGATGAAACGCCAG
AGGTCACCTCCAGCCTGGTCGACGAGTTTGCTTCGGCCGGCCTGGTGAATCTGGTGGGCGGTTGCTGCGGCACCACGCCG
GAGCACATCAAGGCGATCGCCGACCGCGTGGCCAACCGCAAGCCGCGTGCATGGCCCGGCCAGTATCTCGAAGCCGCCTG
A

Upstream 100 bases:

>100_bases
TGCCCACAACGGCGGCAAGTCATTGATGCGACTGGTAAAATTCCGCTTTGGCCGCAAATTCTGAATATTCGACCGGCCGC
TGCACCTCGGAGTCCCTTCC

Downstream 100 bases:

>100_bases
CATGAAGGCGCGCATCGACAGCACCATCCCCGTGCTTGCCTCGCTGGACCTGCAGGAGTCGGCCCGCTTCTATACGGAGC
GGCTGGGGTTCACGCAGGCA

Product: homocysteine S-methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MTAPLPYTRAANLPALLRERILILDGAMGTMIQRYKLNEAQYRGERFANHPIDVKGNNELLLLTRPDVIREIHEQYLAAG
ADLIETNTFGATTIAQEDYKMAELAYEMNVVAARLAREACDKYSTPDKPRFVAGAFGPTPKTASISPDVNDPGARNVSFD
QLRDAYYEQGKALLEGGADVFLVETIFDTLNAKAALFAIDQLFEDTGERVPVMISGTVTDASGRILSGQTVEAFWNSLRH
AKPITFGLNCALGAALMRPYIAELAKICDTAVSCYPNAGLPNPMSETGFDETPEVTSSLVDEFASAGLVNLVGGCCGTTP
EHIKAIADRVANRKPRAWPGQYLEAA

Sequences:

>Translated_346_residues
MTAPLPYTRAANLPALLRERILILDGAMGTMIQRYKLNEAQYRGERFANHPIDVKGNNELLLLTRPDVIREIHEQYLAAG
ADLIETNTFGATTIAQEDYKMAELAYEMNVVAARLAREACDKYSTPDKPRFVAGAFGPTPKTASISPDVNDPGARNVSFD
QLRDAYYEQGKALLEGGADVFLVETIFDTLNAKAALFAIDQLFEDTGERVPVMISGTVTDASGRILSGQTVEAFWNSLRH
AKPITFGLNCALGAALMRPYIAELAKICDTAVSCYPNAGLPNPMSETGFDETPEVTSSLVDEFASAGLVNLVGGCCGTTP
EHIKAIADRVANRKPRAWPGQYLEAA
>Mature_345_residues
TAPLPYTRAANLPALLRERILILDGAMGTMIQRYKLNEAQYRGERFANHPIDVKGNNELLLLTRPDVIREIHEQYLAAGA
DLIETNTFGATTIAQEDYKMAELAYEMNVVAARLAREACDKYSTPDKPRFVAGAFGPTPKTASISPDVNDPGARNVSFDQ
LRDAYYEQGKALLEGGADVFLVETIFDTLNAKAALFAIDQLFEDTGERVPVMISGTVTDASGRILSGQTVEAFWNSLRHA
KPITFGLNCALGAALMRPYIAELAKICDTAVSCYPNAGLPNPMSETGFDETPEVTSSLVDEFASAGLVNLVGGCCGTTPE
HIKAIADRVANRKPRAWPGQYLEAA

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=342, Percent_Identity=53.8011695906433, Blast_Score=374, Evalue=1e-104,
Organism=Escherichia coli, GI1790450, Length=334, Percent_Identity=58.0838323353293, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI1786456, Length=328, Percent_Identity=27.1341463414634, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17535405, Length=329, Percent_Identity=53.4954407294833, Blast_Score=356, Evalue=1e-98,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 37540; Mature: 37409

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPLPYTRAANLPALLRERILILDGAMGTMIQRYKLNEAQYRGERFANHPIDVKGNNEL
CCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHCCCCEEECCCCCE
LLLTRPDVIREIHEQYLAAGADLIETNTFGATTIAQEDYKMAELAYEMNVVAARLAREAC
EEEECCHHHHHHHHHHHHCCCHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKYSTPDKPRFVAGAFGPTPKTASISPDVNDPGARNVSFDQLRDAYYEQGKALLEGGADV
HHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHCCCCE
FLVETIFDTLNAKAALFAIDQLFEDTGERVPVMISGTVTDASGRILSGQTVEAFWNSLRH
EEHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEECCCHHHHHHHHHHH
AKPITFGLNCALGAALMRPYIAELAKICDTAVSCYPNAGLPNPMSETGFDETPEVTSSLV
CCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
DEFASAGLVNLVGGCCGTTPEHIKAIADRVANRKPRAWPGQYLEAA
HHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCC
>Mature Secondary Structure 
TAPLPYTRAANLPALLRERILILDGAMGTMIQRYKLNEAQYRGERFANHPIDVKGNNEL
CCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHCCCCEEECCCCCE
LLLTRPDVIREIHEQYLAAGADLIETNTFGATTIAQEDYKMAELAYEMNVVAARLAREAC
EEEECCHHHHHHHHHHHHCCCHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKYSTPDKPRFVAGAFGPTPKTASISPDVNDPGARNVSFDQLRDAYYEQGKALLEGGADV
HHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHCCCCE
FLVETIFDTLNAKAALFAIDQLFEDTGERVPVMISGTVTDASGRILSGQTVEAFWNSLRH
EEHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEECCCHHHHHHHHHHH
AKPITFGLNCALGAALMRPYIAELAKICDTAVSCYPNAGLPNPMSETGFDETPEVTSSLV
CCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
DEFASAGLVNLVGGCCGTTPEHIKAIADRVANRKPRAWPGQYLEAA
HHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]