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The map label for this gene is dsbA [H]

Identifier: 187927243

GI number: 187927243

Start: 155200

End: 155856

Strand: Reverse

Name: dsbA [H]

Synonym: Rpic_0135

Alternate gene names: 187927243

Gene position: 155856-155200 (Counterclockwise)

Preceding gene: 187927244

Following gene: 187927242

Centisome position: 3.95

GC content: 60.43

Gene sequence:

>657_bases
ATGAAAAAACTCGCTGCTTTCCTGATCGCCCTCGCCACTGGCGCCGGCTTCCTGATGACCGCGCCGGCCAACGCCGCGCC
CACCGCCGGCAAGGAATACAAAGTGCTGCAAACGCCGCAGCCCGTGCCCGCAGGCAAGATCGAAGTGACGGAGTTCTTCT
GGTACGGCTGCCCGCACTGCTATGACTTTGAAAACACCTGGACAGCGTGGGTCGCCAAGCAAGGCAAGGACGTGGTGATC
AAGCGCGTGCCGGTGGCCTTCAATGCCAAGCTGGAGCCGCATACCCGCATCTATTACACGCTGGAAGCGCTCGGCAAGCT
CGACGCCAAGGACGCCAGCGGCAAGACGCTGCATGACCGCGTGTTTGACCAACTGCACAAGAACTACCGTTCGATGTCCG
AATTGGATGACATTGCGAAGTTCATGGCTGCCAACGGCGTCGACGAGAAGCAGTTCCGCGACACCTACAACTCGTTCAGT
GTGAACGCCAATACCAAACGCGCCGCGCAACTGGCCGATCAGTACAAGATCGAAGGCGTGCCGACCGTCGTTGTGCAGGG
TAAATACACCACGTCGCCGGCAGATGCCGGCAGCAACGTGGGCGCTGCCCAGACGCTCGACTACCTCGTCGACCAGGTGC
GCAACCGCAAGATGTGA

Upstream 100 bases:

>100_bases
TCAGTTACGCTGACAGCGGCATCTGTCGGTTCTGCACTGGATTTCGCTGGTTTTTCGGTTGCTTCGTCCAGTTCGTCGTC
TAATTCGTCGTCCATCCACC

Downstream 100 bases:

>100_bases
CGGATTGACGCGTTTCCGGGCATTCGCCCGGGCGTGACACCGCCATCGACGCCCGCCCCTGTGCGGGCGTTTTTTGTGCG
TCGGCCTTTTTCACACCTAC

Product: DSBA oxidoreductase

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MKKLAAFLIALATGAGFLMTAPANAAPTAGKEYKVLQTPQPVPAGKIEVTEFFWYGCPHCYDFENTWTAWVAKQGKDVVI
KRVPVAFNAKLEPHTRIYYTLEALGKLDAKDASGKTLHDRVFDQLHKNYRSMSELDDIAKFMAANGVDEKQFRDTYNSFS
VNANTKRAAQLADQYKIEGVPTVVVQGKYTTSPADAGSNVGAAQTLDYLVDQVRNRKM

Sequences:

>Translated_218_residues
MKKLAAFLIALATGAGFLMTAPANAAPTAGKEYKVLQTPQPVPAGKIEVTEFFWYGCPHCYDFENTWTAWVAKQGKDVVI
KRVPVAFNAKLEPHTRIYYTLEALGKLDAKDASGKTLHDRVFDQLHKNYRSMSELDDIAKFMAANGVDEKQFRDTYNSFS
VNANTKRAAQLADQYKIEGVPTVVVQGKYTTSPADAGSNVGAAQTLDYLVDQVRNRKM
>Mature_218_residues
MKKLAAFLIALATGAGFLMTAPANAAPTAGKEYKVLQTPQPVPAGKIEVTEFFWYGCPHCYDFENTWTAWVAKQGKDVVI
KRVPVAFNAKLEPHTRIYYTLEALGKLDAKDASGKTLHDRVFDQLHKNYRSMSELDDIAKFMAANGVDEKQFRDTYNSFS
VNANTKRAAQLADQYKIEGVPTVVVQGKYTTSPADAGSNVGAAQTLDYLVDQVRNRKM

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins. Involved in the production of protease and alkaline phosphatase, motility, metal resistance, and multi-drug resistance [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioredoxin family. DsbA subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 24134; Mature: 24134

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLAAFLIALATGAGFLMTAPANAAPTAGKEYKVLQTPQPVPAGKIEVTEFFWYGCPHC
CHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCE
YDFENTWTAWVAKQGKDVVIKRVPVAFNAKLEPHTRIYYTLEALGKLDAKDASGKTLHDR
ECCCCCHHHHHHCCCCEEEEEECCEEEECCCCCCCEEEEEHHHHCCCCCCCCCCCHHHHH
VFDQLHKNYRSMSELDDIAKFMAANGVDEKQFRDTYNSFSVNANTKRAAQLADQYKIEGV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCEECCCCHHHHHHHHHHHCCCCC
PTVVVQGKYTTSPADAGSNVGAAQTLDYLVDQVRNRKM
CEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKLAAFLIALATGAGFLMTAPANAAPTAGKEYKVLQTPQPVPAGKIEVTEFFWYGCPHC
CHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCE
YDFENTWTAWVAKQGKDVVIKRVPVAFNAKLEPHTRIYYTLEALGKLDAKDASGKTLHDR
ECCCCCHHHHHHCCCCEEEEEECCEEEECCCCCCCEEEEEHHHHCCCCCCCCCCCHHHHH
VFDQLHKNYRSMSELDDIAKFMAANGVDEKQFRDTYNSFSVNANTKRAAQLADQYKIEGV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCEECCCCHHHHHHHHHHHCCCCC
PTVVVQGKYTTSPADAGSNVGAAQTLDYLVDQVRNRKM
CEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10711598 [H]