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The map label for this gene is ghrA [H]

Identifier: 187927223

GI number: 187927223

Start: 133357

End: 134298

Strand: Reverse

Name: ghrA [H]

Synonym: Rpic_0115

Alternate gene names: 187927223

Gene position: 134298-133357 (Counterclockwise)

Preceding gene: 187927224

Following gene: 187927222

Centisome position: 3.41

GC content: 67.73

Gene sequence:

>942_bases
ATGCACATCCAGATCTACACGCCCGACGGCAAGCCCCAACCCTGGCTCGACGGTTTCGCGCAGGCACTTCCTGAGGCACG
CCTGTCGGTGTGGGAGCAAGGCGCAGTGCAGGACGCCGACTACGCCGTGGTCTGGCAGCCGCCCGCCGACATGCTGCGCG
ATCGCCGCGATTTGCGCGCCGTGTTCAACCTCGGCGCGGGCGTCGATGCCATCCTGGGCCTGCGTGCGCAAGCGCCCGAT
GCCATCCCCGAAGGCCTGCCGATCGTGCGGCTGGACGACGCCGGCATGGCCGCGCAGATGGGCGAATACGTCACGCACGC
CGTCCTGCGCTTCTTCCGCCGGCTCGATGAATACGAGCGCCAGCAGCAAGCGAAGATGTGGCGCTTCCTCAAGCCGTTCA
ACCGCGATGAGTTTGTCGTCGGCGTGCTGGGCCTCGGCGTGCTCGGTACGCACATCGCCAGGACGCTGGCGGGCTTCGGC
TTTCCGGTGCGCGGCTGGAGCCGCTCCGCCAAGCACGTCGAGGGCGTCGATTGCCGCTCAGGCAGCGATGCCCTGCCCGG
CTTCCTCGCCGGCACGCGCGTGCTGGTGAACGTGCTGCCGCTGACGGCAGACACCGAAAACGTGATCGACGCGAAACTGC
TCGCGCAACTCGCACCGGGCGCGTTCGTTGTCAACGTGGCGCGCGGTAAGCATGTCGTGGAAGACGATCTGCTGGCCGCC
GTGCGCAGCGGCCACATTGCCGGCGCCGCGCTCGACGTATTCCGCACCGAGCCGCTGCCGGCCGACCACCCTTTCTGGAC
CGAACCGCGCATCCACATCACGCCGCACATTTCGGCGCTGACGCTGCGCGAGGTCAGCATTGCCCAGATCGCGCGCAAGA
TCCGAGCGCTGGAAGCAGGCGAGCCGATTGCCGGCATCGTCGATCTGCAACGAGGTTACTGA

Upstream 100 bases:

>100_bases
AGCCCGCCGAATGAAGCCCAAGGGGGCTGCCACACGCCTTGGCGGCCCCTGCTACACTGCTTGACGTATACGTCAATCAC
CCGCATTGAAGTCGCCCCAC

Downstream 100 bases:

>100_bases
GATGAGCATTCCGAATTTTGTGAAGGTGGTCGAGGTCGGCCCGCGCGATGGTCTGCAGAACGAGAAGTCGCCAGTTTCGA
CGGACACCAAGGTCGAGCTG

Product: NAD-binding D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MHIQIYTPDGKPQPWLDGFAQALPEARLSVWEQGAVQDADYAVVWQPPADMLRDRRDLRAVFNLGAGVDAILGLRAQAPD
AIPEGLPIVRLDDAGMAAQMGEYVTHAVLRFFRRLDEYERQQQAKMWRFLKPFNRDEFVVGVLGLGVLGTHIARTLAGFG
FPVRGWSRSAKHVEGVDCRSGSDALPGFLAGTRVLVNVLPLTADTENVIDAKLLAQLAPGAFVVNVARGKHVVEDDLLAA
VRSGHIAGAALDVFRTEPLPADHPFWTEPRIHITPHISALTLREVSIAQIARKIRALEAGEPIAGIVDLQRGY

Sequences:

>Translated_313_residues
MHIQIYTPDGKPQPWLDGFAQALPEARLSVWEQGAVQDADYAVVWQPPADMLRDRRDLRAVFNLGAGVDAILGLRAQAPD
AIPEGLPIVRLDDAGMAAQMGEYVTHAVLRFFRRLDEYERQQQAKMWRFLKPFNRDEFVVGVLGLGVLGTHIARTLAGFG
FPVRGWSRSAKHVEGVDCRSGSDALPGFLAGTRVLVNVLPLTADTENVIDAKLLAQLAPGAFVVNVARGKHVVEDDLLAA
VRSGHIAGAALDVFRTEPLPADHPFWTEPRIHITPHISALTLREVSIAQIARKIRALEAGEPIAGIVDLQRGY
>Mature_313_residues
MHIQIYTPDGKPQPWLDGFAQALPEARLSVWEQGAVQDADYAVVWQPPADMLRDRRDLRAVFNLGAGVDAILGLRAQAPD
AIPEGLPIVRLDDAGMAAQMGEYVTHAVLRFFRRLDEYERQQQAKMWRFLKPFNRDEFVVGVLGLGVLGTHIARTLAGFG
FPVRGWSRSAKHVEGVDCRSGSDALPGFLAGTRVLVNVLPLTADTENVIDAKLLAQLAPGAFVVNVARGKHVVEDDLLAA
VRSGHIAGAALDVFRTEPLPADHPFWTEPRIHITPHISALTLREVSIAQIARKIRALEAGEPIAGIVDLQRGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=266, Percent_Identity=29.3233082706767, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI23308577, Length=307, Percent_Identity=28.3387622149837, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI87081824, Length=313, Percent_Identity=46.3258785942492, Blast_Score=294, Evalue=4e-81,
Organism=Escherichia coli, GI87082289, Length=145, Percent_Identity=31.0344827586207, Blast_Score=71, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17532191, Length=232, Percent_Identity=29.7413793103448, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324964, Length=207, Percent_Identity=25.6038647342995, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324980, Length=170, Percent_Identity=27.6470588235294, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28574286, Length=256, Percent_Identity=27.34375, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI45552429, Length=171, Percent_Identity=32.7485380116959, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24585514, Length=171, Percent_Identity=32.7485380116959, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28574282, Length=171, Percent_Identity=32.7485380116959, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28574284, Length=171, Percent_Identity=32.7485380116959, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45551003, Length=171, Percent_Identity=32.7485380116959, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24585516, Length=298, Percent_Identity=25.8389261744966, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI28571528, Length=206, Percent_Identity=28.6407766990291, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 34275; Mature: 34275

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIQIYTPDGKPQPWLDGFAQALPEARLSVWEQGAVQDADYAVVWQPPADMLRDRRDLRA
CEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHH
VFNLGAGVDAILGLRAQAPDAIPEGLPIVRLDDAGMAAQMGEYVTHAVLRFFRRLDEYER
HHHCCCCHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QQQAKMWRFLKPFNRDEFVVGVLGLGVLGTHIARTLAGFGFPVRGWSRSAKHVEGVDCRS
HHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
GSDALPGFLAGTRVLVNVLPLTADTENVIDAKLLAQLAPGAFVVNVARGKHVVEDDLLAA
CCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHH
VRSGHIAGAALDVFRTEPLPADHPFWTEPRIHITPHISALTLREVSIAQIARKIRALEAG
HHCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCC
EPIAGIVDLQRGY
CCCCHHHCCCCCC
>Mature Secondary Structure
MHIQIYTPDGKPQPWLDGFAQALPEARLSVWEQGAVQDADYAVVWQPPADMLRDRRDLRA
CEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHH
VFNLGAGVDAILGLRAQAPDAIPEGLPIVRLDDAGMAAQMGEYVTHAVLRFFRRLDEYER
HHHCCCCHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QQQAKMWRFLKPFNRDEFVVGVLGLGVLGTHIARTLAGFGFPVRGWSRSAKHVEGVDCRS
HHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
GSDALPGFLAGTRVLVNVLPLTADTENVIDAKLLAQLAPGAFVVNVARGKHVVEDDLLAA
CCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHH
VRSGHIAGAALDVFRTEPLPADHPFWTEPRIHITPHISALTLREVSIAQIARKIRALEAG
HHCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCC
EPIAGIVDLQRGY
CCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA