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The map label for this gene is azoR [H]

Identifier: 187934432

GI number: 187934432

Start: 2287191

End: 2287838

Strand: Reverse

Name: azoR [H]

Synonym: CLL_A2201

Alternate gene names: 187934432

Gene position: 2287838-2287191 (Counterclockwise)

Preceding gene: 187932363

Following gene: 187935206

Centisome position: 60.2

GC content: 30.56

Gene sequence:

>648_bases
ATGAAAAAATTACTTTATATAACTGTAAACTCAAAGCCTGAGGAGTTGTCTGCAAGTAAAACTGTAGGAAGGGCTTTTGT
AAATAGATTTTTAGAGAAACACAGTGACTTTGAAATAGAGGAACTAGATTTGTATAAGTGTCATATACCAAGACTTGAAT
ATGAATATTTTGAAAAGAGAAGCTGTCTAGTAAATGAAGACGCATTTAATAAACTGGATAAAAAACAACAAGAAGAAGTT
CATAAAATAGTTAAACTAACAGATCAATTTAAAGAAGCAGATGTATATGTAATTGCTGCACCAATGTGGAGTATGTCTTT
TCCAGCTCCATTAAAAGAATACATTGATTGTATAGTTATGGATGGAAAAACAGTTGATATAAAAGATGGAGAAAAACCAG
AGGGGTTATTAAATGATAAACCAAGAGGTGCTGTATATATACAATCATCTGGAGCAAAAATGAACGTATTACTAAAGGTG
GTGATGGATAAAGGTGTTCATTATATAGAAAGTATAATGAAATTTATGGGCATTGAAAAATTTGAAGAATTATTAGTAGA
TGGAACAGGAACAACAGAAGAAGAAAAAAACAAGGCCATAGAAAAAGCTTTTGAAAAGATAGACTCTGTGATAGATGGTA
TATGGTAA

Upstream 100 bases:

>100_bases
CAAGTTTTTAGTTTTTACAGTTTATTTATTTGTGATAAATGTATTAAGTACACTAAATTGCAAATAATAAAATAAATAGG
TAAATGCAAGGAGTGTTCTT

Downstream 100 bases:

>100_bases
ATCTTTGGGTGATATTATAAATATTTTTGTGCAGTGGAGAGTATAGATAATTTTATATGATTTATAGAGAGTATAGATGA
TTTTGTGTGATTTATATCTT

Product: FMN-dependent NADH-azoreductase family protein

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase; FMN-dependent NADH-azo compound oxidoreductase [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MKKLLYITVNSKPEELSASKTVGRAFVNRFLEKHSDFEIEELDLYKCHIPRLEYEYFEKRSCLVNEDAFNKLDKKQQEEV
HKIVKLTDQFKEADVYVIAAPMWSMSFPAPLKEYIDCIVMDGKTVDIKDGEKPEGLLNDKPRGAVYIQSSGAKMNVLLKV
VMDKGVHYIESIMKFMGIEKFEELLVDGTGTTEEEKNKAIEKAFEKIDSVIDGIW

Sequences:

>Translated_215_residues
MKKLLYITVNSKPEELSASKTVGRAFVNRFLEKHSDFEIEELDLYKCHIPRLEYEYFEKRSCLVNEDAFNKLDKKQQEEV
HKIVKLTDQFKEADVYVIAAPMWSMSFPAPLKEYIDCIVMDGKTVDIKDGEKPEGLLNDKPRGAVYIQSSGAKMNVLLKV
VMDKGVHYIESIMKFMGIEKFEELLVDGTGTTEEEKNKAIEKAFEKIDSVIDGIW
>Mature_215_residues
MKKLLYITVNSKPEELSASKTVGRAFVNRFLEKHSDFEIEELDLYKCHIPRLEYEYFEKRSCLVNEDAFNKLDKKQQEEV
HKIVKLTDQFKEADVYVIAAPMWSMSFPAPLKEYIDCIVMDGKTVDIKDGEKPEGLLNDKPRGAVYIQSSGAKMNVLLKV
VMDKGVHYIESIMKFMGIEKFEELLVDGTGTTEEEKNKAIEKAFEKIDSVIDGIW

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=197, Percent_Identity=30.9644670050761, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 3.1.4.14

Molecular weight: Translated: 24757; Mature: 24757

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLYITVNSKPEELSASKTVGRAFVNRFLEKHSDFEIEELDLYKCHIPRLEYEYFEKR
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEEECCCCCHHHHHHHH
SCLVNEDAFNKLDKKQQEEVHKIVKLTDQFKEADVYVIAAPMWSMSFPAPLKEYIDCIVM
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHEEEE
DGKTVDIKDGEKPEGLLNDKPRGAVYIQSSGAKMNVLLKVVMDKGVHYIESIMKFMGIEK
CCCEEECCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHCHHH
FEELLVDGTGTTEEEKNKAIEKAFEKIDSVIDGIW
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKLLYITVNSKPEELSASKTVGRAFVNRFLEKHSDFEIEELDLYKCHIPRLEYEYFEKR
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEEECCCCCHHHHHHHH
SCLVNEDAFNKLDKKQQEEVHKIVKLTDQFKEADVYVIAAPMWSMSFPAPLKEYIDCIVM
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHEEEE
DGKTVDIKDGEKPEGLLNDKPRGAVYIQSSGAKMNVLLKVVMDKGVHYIESIMKFMGIEK
CCCEEECCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHCHHH
FEELLVDGTGTTEEEKNKAIEKAFEKIDSVIDGIW
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA