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The map label for this gene is rfbX [H]

Identifier: 187933874

GI number: 187933874

Start: 3324220

End: 3325686

Strand: Reverse

Name: rfbX [H]

Synonym: CLL_A3197

Alternate gene names: 187933874

Gene position: 3325686-3324220 (Counterclockwise)

Preceding gene: 187932580

Following gene: 187934100

Centisome position: 87.51

GC content: 24.13

Gene sequence:

>1467_bases
ATGACAAAGTCAATATCGAAAAATGCATTGTTTAAGGGATCATTAAATCTATTTAATATTGTATTACCAATATTAGTGGG
TCCTGTAATAACAAGAACTTTGGGTGAGGATTTATATGGGTATATTTCATATGCAGAATCACTAACAGGATGGTTTTTAA
TATTTGCATGTTTTGGAATTTCTCAATATGGATTGAGGGAAATAAGTAAAGTTAGAGATGATGAAGAAAAGCTTCAACAA
ACATTTACAAGTTTATTTTTATTTACTCTCTTTACCAATATATTAGTGTCTTCAATATACATGATTTTTATATATACAGT
AGGAAGAGGTAGACCAGATTTTTATACTTGTATGATTTTAGGGTTTAATCTTGTTTTTAATCTTTTTTATGTTGAATGGA
TTAATGAGGCATTAGAGAATTACGATTTTATAGCTATAAAGACTATGATTGTTAGAATAATATATTCTTTACTAGTTATT
TTTTGTATAAGAAATATTAATGATTTTGAATTATATTTATACTTAGTTATAGGCTTTAATTTCTTAAATAATATATTAAG
TTTTGTATACATAAAGAAAAGAGTGAATTTTAATTTTAAAAATCTACATTTTAGAAAACATATTAAGCCAATGTTTTTTG
TTGTTATTTTATCTAATGTAAATATTTTATATACTCAATTAGACAAATTAATGATGGGTAGTGGAGCCGTAGGTGCAGGA
ACTAGAGATGTTGGATATTATAGTTTAGCTCAAAAGATAATGACAATAATTAATACATTAATGCTTACTATTATTCAAGT
AACTATGCCTAGACTTTCAAATTATTTAGGTAATGATTCTAAGGAAGAGTACATGTCTCTTTTAAAAAGAATTATAAAGA
TATATTTTATGTTTTTATTCCCAGCATCTATTGGAATGATATGTCTTTCAAAAGAGATAATACTTATGTATGCAGGTCCT
AAATTTATAGCAGCAGCACCTGTAATGTTTATATTTTCAATTTACATGTTAACTATTGGGGTTGAAGGAATAATATCTAA
TCAAATGATATATCTTAATGGAAGAGAAAAAGATGATGTTAAATTAGTATTAATTGGTGGGGTTTTAAATGTTATATTAA
ATATAGGTTTAGCCATAACAGGTAACTTTACACCTACAAACGCAATTATAACAACTCTTATTGCAAATTTAATTGTTATA
GTACTTGAATATAGATTTGTAAGAAAAGTTATTAAAATAGATATAAACTTATTTGCATTTGAAAATGTTAAGTACTTTTA
TTACTCATTATTATTTATTCCACTAATATTTTTGATTAAGCATTTTATAAATAGTGTATTTTTAATTTCAGCATTATCAG
TGTTAGTATGCGGAATAATATATGTAGGAATTTTATTTATTACTAAAGATGAAGTATTTTTACAACTTACAAATACTTTA
CTTAGAAAAGTTAAAATAAAGAGATAA

Upstream 100 bases:

>100_bases
GTATAATATTGCTAAGGACTTAATTTGACAACTTCTTTAGTTAGTGTAAAATATAGTAGAAGTAAAATAATCCATTTAGC
GATTATAGGAGGACTACAAT

Downstream 100 bases:

>100_bases
TACATTGCTAATGTGCTTATAGCTCATACAATTACAATTTTTAGAGTGGTTTTTTAGCTAATTATTACTTTAAAATGAAT
GTGTTTGTATGGGCTATTTT

Product: polysaccharide biosynthesis protein

Products: lipopolysaccharide [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MTKSISKNALFKGSLNLFNIVLPILVGPVITRTLGEDLYGYISYAESLTGWFLIFACFGISQYGLREISKVRDDEEKLQQ
TFTSLFLFTLFTNILVSSIYMIFIYTVGRGRPDFYTCMILGFNLVFNLFYVEWINEALENYDFIAIKTMIVRIIYSLLVI
FCIRNINDFELYLYLVIGFNFLNNILSFVYIKKRVNFNFKNLHFRKHIKPMFFVVILSNVNILYTQLDKLMMGSGAVGAG
TRDVGYYSLAQKIMTIINTLMLTIIQVTMPRLSNYLGNDSKEEYMSLLKRIIKIYFMFLFPASIGMICLSKEIILMYAGP
KFIAAAPVMFIFSIYMLTIGVEGIISNQMIYLNGREKDDVKLVLIGGVLNVILNIGLAITGNFTPTNAIITTLIANLIVI
VLEYRFVRKVIKIDINLFAFENVKYFYYSLLFIPLIFLIKHFINSVFLISALSVLVCGIIYVGILFITKDEVFLQLTNTL
LRKVKIKR

Sequences:

>Translated_488_residues
MTKSISKNALFKGSLNLFNIVLPILVGPVITRTLGEDLYGYISYAESLTGWFLIFACFGISQYGLREISKVRDDEEKLQQ
TFTSLFLFTLFTNILVSSIYMIFIYTVGRGRPDFYTCMILGFNLVFNLFYVEWINEALENYDFIAIKTMIVRIIYSLLVI
FCIRNINDFELYLYLVIGFNFLNNILSFVYIKKRVNFNFKNLHFRKHIKPMFFVVILSNVNILYTQLDKLMMGSGAVGAG
TRDVGYYSLAQKIMTIINTLMLTIIQVTMPRLSNYLGNDSKEEYMSLLKRIIKIYFMFLFPASIGMICLSKEIILMYAGP
KFIAAAPVMFIFSIYMLTIGVEGIISNQMIYLNGREKDDVKLVLIGGVLNVILNIGLAITGNFTPTNAIITTLIANLIVI
VLEYRFVRKVIKIDINLFAFENVKYFYYSLLFIPLIFLIKHFINSVFLISALSVLVCGIIYVGILFITKDEVFLQLTNTL
LRKVKIKR
>Mature_487_residues
TKSISKNALFKGSLNLFNIVLPILVGPVITRTLGEDLYGYISYAESLTGWFLIFACFGISQYGLREISKVRDDEEKLQQT
FTSLFLFTLFTNILVSSIYMIFIYTVGRGRPDFYTCMILGFNLVFNLFYVEWINEALENYDFIAIKTMIVRIIYSLLVIF
CIRNINDFELYLYLVIGFNFLNNILSFVYIKKRVNFNFKNLHFRKHIKPMFFVVILSNVNILYTQLDKLMMGSGAVGAGT
RDVGYYSLAQKIMTIINTLMLTIIQVTMPRLSNYLGNDSKEEYMSLLKRIIKIYFMFLFPASIGMICLSKEIILMYAGPK
FIAAAPVMFIFSIYMLTIGVEGIISNQMIYLNGREKDDVKLVLIGGVLNVILNIGLAITGNFTPTNAIITTLIANLIVIV
LEYRFVRKVIKIDINLFAFENVKYFYYSLLFIPLIFLIKHFINSVFLISALSVLVCGIIYVGILFITKDEVFLQLTNTLL
RKVKIKR

Specific function: May be involved in the translocation process of the nascent O-polysaccharide molecules and/or its ligation to lipid A core units [H]

COG id: COG2244

COG function: function code R; Membrane protein involved in the export of O-antigen and teichoic acid

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788349, Length=416, Percent_Identity=22.5961538461538, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002797 [H]

Pfam domain/function: PF01943 Polysacc_synt [H]

EC number: NA

Molecular weight: Translated: 56107; Mature: 55976

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSISKNALFKGSLNLFNIVLPILVGPVITRTLGEDLYGYISYAESLTGWFLIFACFGI
CCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SQYGLREISKVRDDEEKLQQTFTSLFLFTLFTNILVSSIYMIFIYTVGRGRPDFYTCMIL
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
GFNLVFNLFYVEWINEALENYDFIAIKTMIVRIIYSLLVIFCIRNINDFELYLYLVIGFN
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FLNNILSFVYIKKRVNFNFKNLHFRKHIKPMFFVVILSNVNILYTQLDKLMMGSGAVGAG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCC
TRDVGYYSLAQKIMTIINTLMLTIIQVTMPRLSNYLGNDSKEEYMSLLKRIIKIYFMFLF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
PASIGMICLSKEIILMYAGPKFIAAAPVMFIFSIYMLTIGVEGIISNQMIYLNGREKDDV
HHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCE
KLVLIGGVLNVILNIGLAITGNFTPTNAIITTLIANLIVIVLEYRFVRKVIKIDINLFAF
EEEEHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
ENVKYFYYSLLFIPLIFLIKHFINSVFLISALSVLVCGIIYVGILFITKDEVFLQLTNTL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LRKVKIKR
HHHHHCCC
>Mature Secondary Structure 
TKSISKNALFKGSLNLFNIVLPILVGPVITRTLGEDLYGYISYAESLTGWFLIFACFGI
CCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC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HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lipopolysaccharide [Cytoplasm] [C]

Specific reaction: lipopolysaccharide [Cytoplasm] = lipopolysaccharide [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7517390; 7517391; 9097040; 9278503 [H]