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The map label for this gene is pbuO [H]

Identifier: 187933611

GI number: 187933611

Start: 1350875

End: 1352167

Strand: Reverse

Name: pbuO [H]

Synonym: CLL_A1315

Alternate gene names: 187933611

Gene position: 1352167-1350875 (Counterclockwise)

Preceding gene: 187932744

Following gene: 187933556

Centisome position: 35.58

GC content: 29.23

Gene sequence:

>1293_bases
ATGAATAAATTTTTTAAATTAAAAGAACACAATTCTACAGTAAGGAGGGAGACTATAGCCGCACTTACATCATTTTTTGC
TGCTGTTTATATAATTATTGTAAACTCTAGCATATTAAGTGATTCAGGCATACCTTTAGAACCATTGATTATAGCAACAG
TTTTATCTTCATTAATAGGATGTCTTATAGTCGGTTTTGTTAGTAACGCTCCTCTAATCATTATGCCCGGTATGGGAATC
AATGCACTATTTACTTATACTGTTGTAAAGACTTTAGGTCTTACATTTTATCAAGCACTAGCAGCAGTAATAGTATCAGG
AATTTTATTAACAATAGTTGCACTTACTCCACTTGCTAAAACAATTACAGAAGCTATTCCAAATAATTTAAAGGAAGCTA
TTACAGTTGGTATTGGACTTTTTATTACACTTATTGGATTAGCAAAATCAGGTCTTATAGTATCTGATCCTTCTAATTTA
TTAAAGCTTGGAGACATAACTAGTCCTGAAGTTATCGCATTCATAATTATTATGATTATTACATTAATATTATTTATTAG
AAATGTACCTGGTGCATTCTTGATTTCAATTATCGTAGGTACAATTATTTCTATATTTATGGGAATTGTAGATTTAAGTA
ATCTTACATTTTCTCTTCCTGACTTCGGAGCATATAAAGATATATTCTTTAATCCTGACTTTAGTGCTATATTAACTCCA
AATTTTTGGATTTCTACATTTTCATTAACATTAGTTTTAGTATTTGAAAATATAGGATTATTGCACGGTCAAGTAAATGG
AATGCTAAAGGCTCCTGAAAAATCTGGAAAAGCTTTAACATCAGTAGGATTCTCTGTTATTGCATATGGATTTTTAGGAA
CTTGTCCTCCTGTTTCTACTGTTGAAGGAGCAGCGGGTATTGCCGCTGGCGGAAGAACTGGATTAACATCAATAATAACT
GGATTATTATTTTTGGTTTCATTATTCTTCATACCATTTATCTCAATAATTCCAAATGCAGCATTAGCTCCAATTCTTAT
TATCATAGGAAGTTTAATGTTCCAAAATTTAAAACATTTAGACTTTGATGATTTAACAGAGTTAATACCAGCATTTATAG
TAATAATATTAATTCCATTAACTTATAGTATTGTAGATGGAATAGCCTTTGGATTTATTTTATATCCCATATGTAAATTA
TTTAGTAATAAAAAGAAAGATTTATCAGTAACTATGTGTATAACATCTACTATCTTCCTTGTTTACTTCTTAATTCAAGG
TTTTATTCATTAA

Upstream 100 bases:

>100_bases
TCAATATACATTTATATTGAATTTTTTGTTATGGATGATATAATAAACTTGCACTTTTTGTGCGGGTGGGCGAGGGAGTT
TTTGAAAGTTGGTCGAAAGG

Downstream 100 bases:

>100_bases
TATATAAAATAAAAATGCAGGAGTTAAAAACTCCTGCATTTTTTATGATTTTATTTCAATCTTAACACTCTACTAGAATA
TAACATTATTTCACCCTATA

Product: xanthine/uracil permease family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MNKFFKLKEHNSTVRRETIAALTSFFAAVYIIIVNSSILSDSGIPLEPLIIATVLSSLIGCLIVGFVSNAPLIIMPGMGI
NALFTYTVVKTLGLTFYQALAAVIVSGILLTIVALTPLAKTITEAIPNNLKEAITVGIGLFITLIGLAKSGLIVSDPSNL
LKLGDITSPEVIAFIIIMIITLILFIRNVPGAFLISIIVGTIISIFMGIVDLSNLTFSLPDFGAYKDIFFNPDFSAILTP
NFWISTFSLTLVLVFENIGLLHGQVNGMLKAPEKSGKALTSVGFSVIAYGFLGTCPPVSTVEGAAGIAAGGRTGLTSIIT
GLLFLVSLFFIPFISIIPNAALAPILIIIGSLMFQNLKHLDFDDLTELIPAFIVIILIPLTYSIVDGIAFGFILYPICKL
FSNKKKDLSVTMCITSTIFLVYFLIQGFIH

Sequences:

>Translated_430_residues
MNKFFKLKEHNSTVRRETIAALTSFFAAVYIIIVNSSILSDSGIPLEPLIIATVLSSLIGCLIVGFVSNAPLIIMPGMGI
NALFTYTVVKTLGLTFYQALAAVIVSGILLTIVALTPLAKTITEAIPNNLKEAITVGIGLFITLIGLAKSGLIVSDPSNL
LKLGDITSPEVIAFIIIMIITLILFIRNVPGAFLISIIVGTIISIFMGIVDLSNLTFSLPDFGAYKDIFFNPDFSAILTP
NFWISTFSLTLVLVFENIGLLHGQVNGMLKAPEKSGKALTSVGFSVIAYGFLGTCPPVSTVEGAAGIAAGGRTGLTSIIT
GLLFLVSLFFIPFISIIPNAALAPILIIIGSLMFQNLKHLDFDDLTELIPAFIVIILIPLTYSIVDGIAFGFILYPICKL
FSNKKKDLSVTMCITSTIFLVYFLIQGFIH
>Mature_430_residues
MNKFFKLKEHNSTVRRETIAALTSFFAAVYIIIVNSSILSDSGIPLEPLIIATVLSSLIGCLIVGFVSNAPLIIMPGMGI
NALFTYTVVKTLGLTFYQALAAVIVSGILLTIVALTPLAKTITEAIPNNLKEAITVGIGLFITLIGLAKSGLIVSDPSNL
LKLGDITSPEVIAFIIIMIITLILFIRNVPGAFLISIIVGTIISIFMGIVDLSNLTFSLPDFGAYKDIFFNPDFSAILTP
NFWISTFSLTLVLVFENIGLLHGQVNGMLKAPEKSGKALTSVGFSVIAYGFLGTCPPVSTVEGAAGIAAGGRTGLTSIIT
GLLFLVSLFFIPFISIIPNAALAPILIIIGSLMFQNLKHLDFDDLTELIPAFIVIILIPLTYSIVDGIAFGFILYPICKL
FSNKKKDLSVTMCITSTIFLVYFLIQGFIH

Specific function: Involved in the uptake of the purine bases hypoxanthine and guanine. May work at purine concentrations higher than 100 uM [H]

COG id: COG2252

COG function: function code R; Permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. AzgA purine transporter (TC 2.A.1.40) subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082309, Length=425, Percent_Identity=34.3529411764706, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1790150, Length=425, Percent_Identity=33.6470588235294, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI1790499, Length=438, Percent_Identity=31.9634703196347, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI48994909, Length=437, Percent_Identity=31.350114416476, Blast_Score=184, Evalue=8e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 46081; Mature: 46081

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKFFKLKEHNSTVRRETIAALTSFFAAVYIIIVNSSILSDSGIPLEPLIIATVLSSLIG
CCCCEECHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHH
CLIVGFVSNAPLIIMPGMGINALFTYTVVKTLGLTFYQALAAVIVSGILLTIVALTPLAK
HHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TITEAIPNNLKEAITVGIGLFITLIGLAKSGLIVSDPSNLLKLGDITSPEVIAFIIIMII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHCCCCCHHHHHHHHHHHH
TLILFIRNVPGAFLISIIVGTIISIFMGIVDLSNLTFSLPDFGAYKDIFFNPDFSAILTP
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCEECCCCCEEECC
NFWISTFSLTLVLVFENIGLLHGQVNGMLKAPEKSGKALTSVGFSVIAYGFLGTCPPVST
CHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
VEGAAGIAAGGRTGLTSIITGLLFLVSLFFIPFISIIPNAALAPILIIIGSLMFQNLKHL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
DFDDLTELIPAFIVIILIPLTYSIVDGIAFGFILYPICKLFSNKKKDLSVTMCITSTIFL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
VYFLIQGFIH
HHHHHHHHCC
>Mature Secondary Structure
MNKFFKLKEHNSTVRRETIAALTSFFAAVYIIIVNSSILSDSGIPLEPLIIATVLSSLIG
CCCCEECHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHH
CLIVGFVSNAPLIIMPGMGINALFTYTVVKTLGLTFYQALAAVIVSGILLTIVALTPLAK
HHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TITEAIPNNLKEAITVGIGLFITLIGLAKSGLIVSDPSNLLKLGDITSPEVIAFIIIMII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHCCCCCHHHHHHHHHHHH
TLILFIRNVPGAFLISIIVGTIISIFMGIVDLSNLTFSLPDFGAYKDIFFNPDFSAILTP
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCEECCCCCEEECC
NFWISTFSLTLVLVFENIGLLHGQVNGMLKAPEKSGKALTSVGFSVIAYGFLGTCPPVST
CHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
VEGAAGIAAGGRTGLTSIITGLLFLVSLFFIPFISIIPNAALAPILIIIGSLMFQNLKHL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
DFDDLTELIPAFIVIILIPLTYSIVDGIAFGFILYPICKLFSNKKKDLSVTMCITSTIFL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
VYFLIQGFIH
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 11591660 [H]