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The map label for this gene is yhcR [H]

Identifier: 187933128

GI number: 187933128

Start: 1927533

End: 1929161

Strand: Reverse

Name: yhcR [H]

Synonym: CLL_A1838

Alternate gene names: 187933128

Gene position: 1929161-1927533 (Counterclockwise)

Preceding gene: 187935682

Following gene: 187933766

Centisome position: 50.76

GC content: 30.51

Gene sequence:

>1629_bases
GTGAAAAGATTATTTAAATCTAAGAAAATAATCTCATTATTGACTACACTAAGTTTAGTATTTACATTAATAACAGGTTT
TCCAGTAGCTGCTTATGCACAGGAATCTGATAAAGTATTTAACTTAGTTGAAATTACAGATTTCCATGGTGCTCTAACTG
ATTCATCTGGAAATCCAGTTGCAGCAGTTCTAGCTGATAGATTAAATGAGATAAAACAATCTAACCCAGAAAGAACTTTA
ATTATGAGTGGTGGAGATTTATATCAAGGATCAGCAACATCTAATATTATGAAAGGGGTTCCAGTACAAGAAGTTATGAC
TAAAATTGGATTAGAAGTAACAGCGCTTGGAAATCACGAATTTGACTGGGGGCTAAACACAATAATAGATACTACAATGA
AGGGGGCAGGATACTCAATAGTTTGTAGCAATCTTTATAATAAGAATACTGGTAAAAGAGTATTTGATCCTTATAAAGTT
GTGACTAAGGATGGAATAAAAATAGCTATAATAGGTGGAATTACAACTGAAACACCATCAATTGTTTTACCTGATAACAT
AAAAGATTATGAATTCAGAGATTTAACAAATGAAATAAATTCAGTTGCAAAAGAAATTAAACAAAATAATTTAGCAGATG
TAGTATTAGTATTAGTTCATGAAGGTGATAACAGAGATAATGCTACAGGACCAATTTTTGATATGGCAAATCAATTAAAT
AATGTAGATGCAATTTTTGGTGGACATACTCATTCTAAAGTTTGTGGAAGTGCTGCCAATACAAAAATTCCAGTATACAT
CGGAAATTCTTATGGAAAAGGTTATATAAACACTAAAGTTACAATAACTAAAGATAAAAAAATTGAATTTGAAAAACCAA
CTTATGAAAATTCTTATGTGGCTTTAGATAATGAAATCGGTTATAAATCTATTAATCCAAAAGTAAGTTCAGAAATAGAA
CAAATGGTTAGAAAAGCTGATGAAACTACAAATCCAATAACTCAAGAAGTTATTGGACTAAATACTGAAAAAGAACTTAC
TAGAATTCAACAAGCTTCTCCATATGGTTCATCTGTTTTAGGAAATTGGGCAGCTGATATTACTAAAGATACAGCAGGTG
CTGAAGTTGGGTTTCAAAATAATGGTGGTTTAAGAATTGATATACCAAAAGGTGAAATTACAGTAGGTACTATGTGGAAA
TTCATGCCTTTTGATAATACAGTTTATAAATTAAATATGAAAAAGTCTGATATTAAAGAAGTATTGGAACAAGCAGTAGG
TGAAGGTGGAAAAGGACTACAAATTTCAGGTATAAAATTCACCTATGATTCAAGCAGACCATCAGGAAATAGAGTTATTG
ATATAACTAGAGATAATGGAGCTTCAATAAGTGATACTGAAGTATTAACTGCAGGAGCACCGGACTTTTTAGCTACAGGT
GGGGATGGATTTAAAGCTTTTACAAAGTGTGGAGGATCTGATCCATCTAATGATACTCATATTGTAATAAGAGATGCGTT
TATTGATTGGTGTAGAGCTAATAAAGATGCTAATGGTAAAAATACGATTCCTAATTCTAATAATAATAGAATAATAAATA
AAATGGTTTTAGAAAAAATAGCTGCTTAA

Upstream 100 bases:

>100_bases
TATAAATTTAAAATGGAAAGCATTACATATTTGGTATATAATTACTTTATCGAGTAATTTTATTTGAAATATAAAATATA
ATAAAGGGGGAATTAGTACT

Downstream 100 bases:

>100_bases
AAATAAAAAGATGATTCAAAATTTAATTTTGAATCATCTTTTTATTATTGAGTAAAATCTAATATTATACACTTAAATCA
ATAGATTAGAATATTAATCA

Product: 2,3-cyclic-nucleotide 2'phosphodiesterase

Products: nucleoside 3'-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MKRLFKSKKIISLLTTLSLVFTLITGFPVAAYAQESDKVFNLVEITDFHGALTDSSGNPVAAVLADRLNEIKQSNPERTL
IMSGGDLYQGSATSNIMKGVPVQEVMTKIGLEVTALGNHEFDWGLNTIIDTTMKGAGYSIVCSNLYNKNTGKRVFDPYKV
VTKDGIKIAIIGGITTETPSIVLPDNIKDYEFRDLTNEINSVAKEIKQNNLADVVLVLVHEGDNRDNATGPIFDMANQLN
NVDAIFGGHTHSKVCGSAANTKIPVYIGNSYGKGYINTKVTITKDKKIEFEKPTYENSYVALDNEIGYKSINPKVSSEIE
QMVRKADETTNPITQEVIGLNTEKELTRIQQASPYGSSVLGNWAADITKDTAGAEVGFQNNGGLRIDIPKGEITVGTMWK
FMPFDNTVYKLNMKKSDIKEVLEQAVGEGGKGLQISGIKFTYDSSRPSGNRVIDITRDNGASISDTEVLTAGAPDFLATG
GDGFKAFTKCGGSDPSNDTHIVIRDAFIDWCRANKDANGKNTIPNSNNNRIINKMVLEKIAA

Sequences:

>Translated_542_residues
MKRLFKSKKIISLLTTLSLVFTLITGFPVAAYAQESDKVFNLVEITDFHGALTDSSGNPVAAVLADRLNEIKQSNPERTL
IMSGGDLYQGSATSNIMKGVPVQEVMTKIGLEVTALGNHEFDWGLNTIIDTTMKGAGYSIVCSNLYNKNTGKRVFDPYKV
VTKDGIKIAIIGGITTETPSIVLPDNIKDYEFRDLTNEINSVAKEIKQNNLADVVLVLVHEGDNRDNATGPIFDMANQLN
NVDAIFGGHTHSKVCGSAANTKIPVYIGNSYGKGYINTKVTITKDKKIEFEKPTYENSYVALDNEIGYKSINPKVSSEIE
QMVRKADETTNPITQEVIGLNTEKELTRIQQASPYGSSVLGNWAADITKDTAGAEVGFQNNGGLRIDIPKGEITVGTMWK
FMPFDNTVYKLNMKKSDIKEVLEQAVGEGGKGLQISGIKFTYDSSRPSGNRVIDITRDNGASISDTEVLTAGAPDFLATG
GDGFKAFTKCGGSDPSNDTHIVIRDAFIDWCRANKDANGKNTIPNSNNNRIINKMVLEKIAA
>Mature_542_residues
MKRLFKSKKIISLLTTLSLVFTLITGFPVAAYAQESDKVFNLVEITDFHGALTDSSGNPVAAVLADRLNEIKQSNPERTL
IMSGGDLYQGSATSNIMKGVPVQEVMTKIGLEVTALGNHEFDWGLNTIIDTTMKGAGYSIVCSNLYNKNTGKRVFDPYKV
VTKDGIKIAIIGGITTETPSIVLPDNIKDYEFRDLTNEINSVAKEIKQNNLADVVLVLVHEGDNRDNATGPIFDMANQLN
NVDAIFGGHTHSKVCGSAANTKIPVYIGNSYGKGYINTKVTITKDKKIEFEKPTYENSYVALDNEIGYKSINPKVSSEIE
QMVRKADETTNPITQEVIGLNTEKELTRIQQASPYGSSVLGNWAADITKDTAGAEVGFQNNGGLRIDIPKGEITVGTMWK
FMPFDNTVYKLNMKKSDIKEVLEQAVGEGGKGLQISGIKFTYDSSRPSGNRVIDITRDNGASISDTEVLTAGAPDFLATG
GDGFKAFTKCGGSDPSNDTHIVIRDAFIDWCRANKDANGKNTIPNSNNNRIINKMVLEKIAA

Specific function: Sugar-nonspecific endonuclease that yields nucleotide 3'-monophosphate products. No 5'-nucleotidase activity was detected, using 5'-AMP as the substrate, in the presence of diverse divalent metals and with various pH values [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TNase-like domain [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=479, Percent_Identity=25.0521920668058, Blast_Score=108, Evalue=2e-23,
Organism=Escherichia coli, GI1786687, Length=527, Percent_Identity=24.0986717267552, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI1790658, Length=249, Percent_Identity=28.5140562248996, Blast_Score=76, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24641187, Length=531, Percent_Identity=25.6120527306968, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19921980, Length=426, Percent_Identity=22.3004694835681, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24652512, Length=426, Percent_Identity=22.3004694835681, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI161076508, Length=426, Percent_Identity=22.3004694835681, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19922444, Length=473, Percent_Identity=22.1987315010571, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI28573524, Length=503, Percent_Identity=22.6640159045726, Blast_Score=91, Evalue=3e-18,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019948
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR016027
- InterPro:   IPR004365
- InterPro:   IPR016071
- InterPro:   IPR006021 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00746 Gram_pos_anchor; PF00149 Metallophos; PF00565 SNase; PF01336 tRNA_anti [H]

EC number: 3.1.4.16

Molecular weight: Translated: 58953; Mature: 58953

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLFKSKKIISLLTTLSLVFTLITGFPVAAYAQESDKVFNLVEITDFHGALTDSSGNPV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEEEEEEEECCCEEECCCCCCH
AAVLADRLNEIKQSNPERTLIMSGGDLYQGSATSNIMKGVPVQEVMTKIGLEVTALGNHE
HHHHHHHHHHHHHCCCCEEEEEECCCEECCCCHHHHHCCCCHHHHHHHHCCEEEEECCCC
FDWGLNTIIDTTMKGAGYSIVCSNLYNKNTGKRVFDPYKVVTKDGIKIAIIGGITTETPS
CCCCHHHHHHHHCCCCCCEEEEECHHCCCCCCCCCCCHHEEECCCEEEEEEECCCCCCCC
IVLPDNIKDYEFRDLTNEINSVAKEIKQNNLADVVLVLVHEGDNRDNATGPIFDMANQLN
EEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHC
NVDAIFGGHTHSKVCGSAANTKIPVYIGNSYGKGYINTKVTITKDKKIEFEKPTYENSYV
CCEEEECCCCHHHHHCCCCCCEEEEEECCCCCCCEEEEEEEEEECCEEEECCCCCCCCEE
ALDNEIGYKSINPKVSSEIEQMVRKADETTNPITQEVIGLNTEKELTRIQQASPYGSSVL
EEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHCCCCCCHHH
GNWAADITKDTAGAEVGFQNNGGLRIDIPKGEITVGTMWKFMPFDNTVYKLNMKKSDIKE
HHHHHHHCCCCCCCEECCCCCCCEEEEECCCCEEEEEEEEEECCCCEEEEEECCHHHHHH
VLEQAVGEGGKGLQISGIKFTYDSSRPSGNRVIDITRDNGASISDTEVLTAGAPDFLATG
HHHHHHCCCCCCEEEEEEEEEECCCCCCCCEEEEEECCCCCCCCCCEEEECCCCHHEECC
GDGFKAFTKCGGSDPSNDTHIVIRDAFIDWCRANKDANGKNTIPNSNNNRIINKMVLEKI
CCCHHHHHHCCCCCCCCCCEEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AA
CC
>Mature Secondary Structure
MKRLFKSKKIISLLTTLSLVFTLITGFPVAAYAQESDKVFNLVEITDFHGALTDSSGNPV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEEEEEEEECCCEEECCCCCCH
AAVLADRLNEIKQSNPERTLIMSGGDLYQGSATSNIMKGVPVQEVMTKIGLEVTALGNHE
HHHHHHHHHHHHHCCCCEEEEEECCCEECCCCHHHHHCCCCHHHHHHHHCCEEEEECCCC
FDWGLNTIIDTTMKGAGYSIVCSNLYNKNTGKRVFDPYKVVTKDGIKIAIIGGITTETPS
CCCCHHHHHHHHCCCCCCEEEEECHHCCCCCCCCCCCHHEEECCCEEEEEEECCCCCCCC
IVLPDNIKDYEFRDLTNEINSVAKEIKQNNLADVVLVLVHEGDNRDNATGPIFDMANQLN
EEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHC
NVDAIFGGHTHSKVCGSAANTKIPVYIGNSYGKGYINTKVTITKDKKIEFEKPTYENSYV
CCEEEECCCCHHHHHCCCCCCEEEEEECCCCCCCEEEEEEEEEECCEEEECCCCCCCCEE
ALDNEIGYKSINPKVSSEIEQMVRKADETTNPITQEVIGLNTEKELTRIQQASPYGSSVL
EEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHCCCCCCHHH
GNWAADITKDTAGAEVGFQNNGGLRIDIPKGEITVGTMWKFMPFDNTVYKLNMKKSDIKE
HHHHHHHCCCCCCCEECCCCCCCEEEEECCCCEEEEEEEEEECCCCEEEEEECCHHHHHH
VLEQAVGEGGKGLQISGIKFTYDSSRPSGNRVIDITRDNGASISDTEVLTAGAPDFLATG
HHHHHHCCCCCCEEEEEEEEEECCCCCCCCEEEEEECCCCCCCCCCEEEECCCCHHEECC
GDGFKAFTKCGGSDPSNDTHIVIRDAFIDWCRANKDANGKNTIPNSNNNRIINKMVLEKI
CCCHHHHHHCCCCCCCCCCEEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nucleoside 2',3'-cyclic phosphate; H2O

Specific reaction: nucleoside 2',3'-cyclic phosphate + H2O = nucleoside 3'-phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]