Click here to switch to the map view.

The map label for this gene is amiB [H]

Identifier: 187918522

GI number: 187918522

Start: 710087

End: 711112

Strand: Direct

Name: amiB [H]

Synonym: BH0666

Alternate gene names: 187918522

Gene position: 710087-711112 (Clockwise)

Preceding gene: 187918521

Following gene: 187918523

Centisome position: 76.99

GC content: 29.04

Gene sequence:

>1026_bases
GTGCAATTGAACTTTGGGAAAATAATATTATTGATTAATTTAATCTTTGTCTTTCTATTTTTAGATGCCCGAGAATTTGA
TTATGTTAATATTCTTGATTCATTTGACAGTAGGCGCTTTAAGTTTAACTTTAACATTGAAAATGATATTCTTACAATTG
AACATGAGAAGGGACATCTTAAGCTTAAGGTGGGATTTGAATATGGCCTTTTGTCTATTGGTTATTATATTTATGTAGAT
CCTATCCTTTTAAAGAATGGGGAAATCTTGATAACCAAGAGGGCTTTGATGCAGATTAAAAATTATTTTAGCTCTTTGCA
AAGCTACAGCAAGCCACGAATAACATCGATAGTAATCGATCCTGGTCATGGTGGACATGATAGAGGAGCTGTTGTGACTC
ATAAAATAAATGAACATAATATTACTCTTTTAGAGAAGGATTTTTCTTTAACTTATTCTATTTATTTATATAAAATATTG
AGTAATTATTTTTTAGATAGGAATATTTTGTTAACGCGTGTAGATGATGTTTTCGTGTCGTTACAAGACAGATCTGAACT
TGCAAATGCAATTAAACCAGATTTTCCAAATAATGTTATATTTTTATCAATGCATGTAAATAATGCGCCAAATCCTGAAG
CTAGGGGAATTGAATTTTGGTATCTTCCTCAGGATTCAAAAAGAGAAGTTGTAAGAAATTTTAAAGGATATGATATTAGA
GGTAATAGATACTTAAGGGAGCTTAATGATATACTAGATATTCAGTATAAATATGAGTCAAAGAAATTAGCTGAGATTTT
ATATGAGACTTTTAGTGATGTTTTATATGAAACCAAGATACGATCAATTAGGGAAGAGCAGTGGTTTGTGATCAAAAATA
GTAGTATGCCTGCTGTGTTGATTGAGATTGGGTTTTTATCTAATATTGATGATGCGATGTTAATTTTAGATTACAATTAT
ATGAGTAAGATGAATATGTTAGTACTTAAATCTTTAATTAAGTTTATTGAATTTTATGAAAAATGA

Upstream 100 bases:

>100_bases
TTAAGGTATGGTGTTAACCCTTTTAAGTTTGGTAGAGAATTTAAAGAGATCAAACATGAAACTATTAAGAATAGTTTAAG
GTTTAAAGGTGACTTAAAGA

Downstream 100 bases:

>100_bases
TGTTTTGGATATGATTTTAAAAAATAATTCTATTTATTGTAATTTTCTTGGTCGTAATGGAGAGAAATATTTACTCGGAA
TATTAATTGTACTTTTATTG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MQLNFGKIILLINLIFVFLFLDAREFDYVNILDSFDSRRFKFNFNIENDILTIEHEKGHLKLKVGFEYGLLSIGYYIYVD
PILLKNGEILITKRALMQIKNYFSSLQSYSKPRITSIVIDPGHGGHDRGAVVTHKINEHNITLLEKDFSLTYSIYLYKIL
SNYFLDRNILLTRVDDVFVSLQDRSELANAIKPDFPNNVIFLSMHVNNAPNPEARGIEFWYLPQDSKREVVRNFKGYDIR
GNRYLRELNDILDIQYKYESKKLAEILYETFSDVLYETKIRSIREEQWFVIKNSSMPAVLIEIGFLSNIDDAMLILDYNY
MSKMNMLVLKSLIKFIEFYEK

Sequences:

>Translated_341_residues
MQLNFGKIILLINLIFVFLFLDAREFDYVNILDSFDSRRFKFNFNIENDILTIEHEKGHLKLKVGFEYGLLSIGYYIYVD
PILLKNGEILITKRALMQIKNYFSSLQSYSKPRITSIVIDPGHGGHDRGAVVTHKINEHNITLLEKDFSLTYSIYLYKIL
SNYFLDRNILLTRVDDVFVSLQDRSELANAIKPDFPNNVIFLSMHVNNAPNPEARGIEFWYLPQDSKREVVRNFKGYDIR
GNRYLRELNDILDIQYKYESKKLAEILYETFSDVLYETKIRSIREEQWFVIKNSSMPAVLIEIGFLSNIDDAMLILDYNY
MSKMNMLVLKSLIKFIEFYEK
>Mature_341_residues
MQLNFGKIILLINLIFVFLFLDAREFDYVNILDSFDSRRFKFNFNIENDILTIEHEKGHLKLKVGFEYGLLSIGYYIYVD
PILLKNGEILITKRALMQIKNYFSSLQSYSKPRITSIVIDPGHGGHDRGAVVTHKINEHNITLLEKDFSLTYSIYLYKIL
SNYFLDRNILLTRVDDVFVSLQDRSELANAIKPDFPNNVIFLSMHVNNAPNPEARGIEFWYLPQDSKREVVRNFKGYDIR
GNRYLRELNDILDIQYKYESKKLAEILYETFSDVLYETKIRSIREEQWFVIKNSSMPAVLIEIGFLSNIDDAMLILDYNY
MSKMNMLVLKSLIKFIEFYEK

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=272, Percent_Identity=23.8970588235294, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1790611, Length=234, Percent_Identity=25.2136752136752, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR018392
- InterPro:   IPR002482 [H]

Pfam domain/function: PF01520 Amidase_3; PF01476 LysM [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 40199; Mature: 40199

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLNFGKIILLINLIFVFLFLDAREFDYVNILDSFDSRRFKFNFNIENDILTIEHEKGHL
CCCCHHHHHHHHHHHHHHHHHCCCCCCEEEHHHCCCCCEEEEEEEECCCEEEEEECCCEE
KLKVGFEYGLLSIGYYIYVDPILLKNGEILITKRALMQIKNYFSSLQSYSKPRITSIVID
EEEEECEECHHHCCEEEEEEEEEEECCCEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEEE
PGHGGHDRGAVVTHKINEHNITLLEKDFSLTYSIYLYKILSNYFLDRNILLTRVDDVFVS
CCCCCCCCCEEEEEEECCCCEEEEECCCCEEHHHHHHHHHHHHHCCCCEEEEEECCCEEE
LQDRSELANAIKPDFPNNVIFLSMHVNNAPNPEARGIEFWYLPQDSKREVVRNFKGYDIR
EHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHCCCCCCCC
GNRYLRELNDILDIQYKYESKKLAEILYETFSDVLYETKIRSIREEQWFVIKNSSMPAVL
CHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEE
IEIGFLSNIDDAMLILDYNYMSKMNMLVLKSLIKFIEFYEK
EEEECCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQLNFGKIILLINLIFVFLFLDAREFDYVNILDSFDSRRFKFNFNIENDILTIEHEKGHL
CCCCHHHHHHHHHHHHHHHHHCCCCCCEEEHHHCCCCCEEEEEEEECCCEEEEEECCCEE
KLKVGFEYGLLSIGYYIYVDPILLKNGEILITKRALMQIKNYFSSLQSYSKPRITSIVID
EEEEECEECHHHCCEEEEEEEEEEECCCEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEEE
PGHGGHDRGAVVTHKINEHNITLLEKDFSLTYSIYLYKILSNYFLDRNILLTRVDDVFVS
CCCCCCCCCEEEEEEECCCCEEEEECCCCEEHHHHHHHHHHHHHCCCCEEEEEECCCEEE
LQDRSELANAIKPDFPNNVIFLSMHVNNAPNPEARGIEFWYLPQDSKREVVRNFKGYDIR
EHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHCCCCCCCC
GNRYLRELNDILDIQYKYESKKLAEILYETFSDVLYETKIRSIREEQWFVIKNSSMPAVL
CHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEE
IEIGFLSNIDDAMLILDYNYMSKMNMLVLKSLIKFIEFYEK
EEEECCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]