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The map label for this gene is oppA [H]

Identifier: 187918200

GI number: 187918200

Start: 339202

End: 340803

Strand: Direct

Name: oppA [H]

Synonym: BH0328

Alternate gene names: 187918200

Gene position: 339202-340803 (Clockwise)

Preceding gene: 187918196

Following gene: 187918201

Centisome position: 36.78

GC content: 33.02

Gene sequence:

>1602_bases
ATGAAATACAAAATATTAATTATTTTATCTTTTTTGATCGTGTGGCTAACTTCTTGTTCTGGCGATGAGCACAAAAATAA
AATAACATTTAGGGTAACAAATGAAGTTGAACCAGATTCACTTGATCCTCAACTTGCTACTTCTGTTCAATCGTTCAACA
TAATTATGAACATATTTTCAGGCTTAACGACAAGAGAGACACAAACTGGTGGATATAAACCAGGACTAGCTAAATCTTGG
GATATATCTGCAGATGGACTTGTATACACACTTCACTTGAGAGAAGATCTTGTTTGGAGTGATGGGGTACCTATTACTGC
TGAAGGAATTCGAAAATCTTATCTTAGAGTTTTAAATAAAGAAACAGCTTCACAATATGTTGATATTGTTAAATCAGCAA
TCAAAAATGCACATGATTACTTTGAAGGTAAAATACCGGAATCTGAACTTGGCATTAAAGCTATAGATAATTCAACTTTA
GAGATAACTTTAATTGCTCCAAAGCCTTATTTTCTTGATATGCTAGTACATCAAACATTTATACCAGTACCAGTTCATGT
CATTGAAAAACATGGACGAAACTGGACAAATCCTGAAAATATAGTAACAAGTGGTGCATACAAATTAAAGACAAGAATCC
CGAATGAAAAAATAGTGCTTGAAAAAAACGATAAGTATTATAATGCCTCAAATGTCGAAATAGATGAAGTGATATTTTAT
CAGGTACAAGGAAACACCGCTTATAACATGTACGTAAATGACGAGCTTGATTTTTTAATGAACGTAGCATCAGAATACTT
AGATGAAGCTAGAATAAGGAATGATTACTATTCCCATCCTATAAATAGAGTAGTCTACACGGCATTTAATACCACTATCA
AACCACTTGATAATGCAAAGGTTAGAGAAGCTTTAACTCTTGCCATTGATAGAGAAGCACTTAACAAAATTACTCTAAAG
GGCCAATCAAAACCAACGAGAAACTTAACCCCACCATTTAAAAACTATTCTTACGACAAAAAATTAAAATTATTTGACCC
ACAAAGAGCAAAACAACTAATGGCTGAGGCTGGTTATCCTGATGGTGCTGGTTTTCCTACTCTTAAATTTAAAACCTCAC
AACGCAATGGAATGGCAATAAGTGCAGAATTCTTGCAAGAACAACTTAAAAAAATACTAAACATTAATATCGATATTGAA
ATTGAAGAATGGAATACATTCCTAAGTAGCAGAAGCACAGGCAATTATCAAATGTCATACATGGGATGGACAGGGGATTA
CTTAGACCCATTAACATTCCTTGAAAGTTTATTTACAACAGAAAATCAAGGATTTGGAGCATATGGATATTCAAACAAAC
AATATGACAATTTAATAAAACAATCAAATTTTGCAAAAGACCCACTACAAAGACAAGAAATCTTAAGACAAGCTGAGGCA
ATAATTACAGAAAAAGACTTCCCCGTTGCCCCACTCTCAATACTTCAATCATACTATCTCTTCAGACATGACAAATGGAC
TGGTTGGACTCCTAATGTTGCAGAAGCTTATCTTTATGAAGAAATAAAATACAAAAGAGAAAACACTAACAATAAAATAT
GA

Upstream 100 bases:

>100_bases
TACTTTATTTATTTATCAAATTAAAGTAAAATCCATTCAAGTATATTAAACAACAATAATTCAAAAGCAAAATAAAAAAT
GTTTATAAGGGGAATAAATT

Downstream 100 bases:

>100_bases
AAACAATTTCATAAGTTAATAGATTAACGCTAATAATCATAAAAGAACGACCACTTCTATACACCAAACTATAGAAGTGG
TTTTTATCGCATAACCACAT

Product: oligopeptide-binding protein OppA

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MKYKILIILSFLIVWLTSCSGDEHKNKITFRVTNEVEPDSLDPQLATSVQSFNIIMNIFSGLTTRETQTGGYKPGLAKSW
DISADGLVYTLHLREDLVWSDGVPITAEGIRKSYLRVLNKETASQYVDIVKSAIKNAHDYFEGKIPESELGIKAIDNSTL
EITLIAPKPYFLDMLVHQTFIPVPVHVIEKHGRNWTNPENIVTSGAYKLKTRIPNEKIVLEKNDKYYNASNVEIDEVIFY
QVQGNTAYNMYVNDELDFLMNVASEYLDEARIRNDYYSHPINRVVYTAFNTTIKPLDNAKVREALTLAIDREALNKITLK
GQSKPTRNLTPPFKNYSYDKKLKLFDPQRAKQLMAEAGYPDGAGFPTLKFKTSQRNGMAISAEFLQEQLKKILNINIDIE
IEEWNTFLSSRSTGNYQMSYMGWTGDYLDPLTFLESLFTTENQGFGAYGYSNKQYDNLIKQSNFAKDPLQRQEILRQAEA
IITEKDFPVAPLSILQSYYLFRHDKWTGWTPNVAEAYLYEEIKYKRENTNNKI

Sequences:

>Translated_533_residues
MKYKILIILSFLIVWLTSCSGDEHKNKITFRVTNEVEPDSLDPQLATSVQSFNIIMNIFSGLTTRETQTGGYKPGLAKSW
DISADGLVYTLHLREDLVWSDGVPITAEGIRKSYLRVLNKETASQYVDIVKSAIKNAHDYFEGKIPESELGIKAIDNSTL
EITLIAPKPYFLDMLVHQTFIPVPVHVIEKHGRNWTNPENIVTSGAYKLKTRIPNEKIVLEKNDKYYNASNVEIDEVIFY
QVQGNTAYNMYVNDELDFLMNVASEYLDEARIRNDYYSHPINRVVYTAFNTTIKPLDNAKVREALTLAIDREALNKITLK
GQSKPTRNLTPPFKNYSYDKKLKLFDPQRAKQLMAEAGYPDGAGFPTLKFKTSQRNGMAISAEFLQEQLKKILNINIDIE
IEEWNTFLSSRSTGNYQMSYMGWTGDYLDPLTFLESLFTTENQGFGAYGYSNKQYDNLIKQSNFAKDPLQRQEILRQAEA
IITEKDFPVAPLSILQSYYLFRHDKWTGWTPNVAEAYLYEEIKYKRENTNNKI
>Mature_533_residues
MKYKILIILSFLIVWLTSCSGDEHKNKITFRVTNEVEPDSLDPQLATSVQSFNIIMNIFSGLTTRETQTGGYKPGLAKSW
DISADGLVYTLHLREDLVWSDGVPITAEGIRKSYLRVLNKETASQYVDIVKSAIKNAHDYFEGKIPESELGIKAIDNSTL
EITLIAPKPYFLDMLVHQTFIPVPVHVIEKHGRNWTNPENIVTSGAYKLKTRIPNEKIVLEKNDKYYNASNVEIDEVIFY
QVQGNTAYNMYVNDELDFLMNVASEYLDEARIRNDYYSHPINRVVYTAFNTTIKPLDNAKVREALTLAIDREALNKITLK
GQSKPTRNLTPPFKNYSYDKKLKLFDPQRAKQLMAEAGYPDGAGFPTLKFKTSQRNGMAISAEFLQEQLKKILNINIDIE
IEEWNTFLSSRSTGNYQMSYMGWTGDYLDPLTFLESLFTTENQGFGAYGYSNKQYDNLIKQSNFAKDPLQRQEILRQAEA
IITEKDFPVAPLSILQSYYLFRHDKWTGWTPNVAEAYLYEEIKYKRENTNNKI

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=480, Percent_Identity=34.1666666666667, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1789397, Length=478, Percent_Identity=32.8451882845188, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI87081878, Length=477, Percent_Identity=31.0272536687631, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1789966, Length=527, Percent_Identity=26.1859582542694, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1787052, Length=466, Percent_Identity=26.3948497854077, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1789887, Length=500, Percent_Identity=26.2, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787551, Length=456, Percent_Identity=21.7105263157895, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1787762, Length=468, Percent_Identity=22.6495726495727, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61253; Mature: 61253

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYKILIILSFLIVWLTSCSGDEHKNKITFRVTNEVEPDSLDPQLATSVQSFNIIMNIFS
CCEEEHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHC
GLTTRETQTGGYKPGLAKSWDISADGLVYTLHLREDLVWSDGVPITAEGIRKSYLRVLNK
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEECCCCCEEHHHHHHHHHHHHHH
ETASQYVDIVKSAIKNAHDYFEGKIPESELGIKAIDNSTLEITLIAPKPYFLDMLVHQTF
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCEEEECCCEEEEEEECCCHHHHHHHHHHHC
IPVPVHVIEKHGRNWTNPENIVTSGAYKLKTRIPNEKIVLEKNDKYYNASNVEIDEVIFY
CCCHHHHHHHCCCCCCCHHHHEECCCEEEEEECCCCEEEEECCCCEECCCCCEEEEEEEE
QVQGNTAYNMYVNDELDFLMNVASEYLDEARIRNDYYSHPINRVVYTAFNTTIKPLDNAK
EEECCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCEECCCCCHH
VREALTLAIDREALNKITLKGQSKPTRNLTPPFKNYSYDKKLKLFDPQRAKQLMAEAGYP
HHHHHHHHCCHHHHHEEEECCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCC
DGAGFPTLKFKTSQRNGMAISAEFLQEQLKKILNINIDIEIEEWNTFLSSRSTGNYQMSY
CCCCCCEEEEECCCCCCCEEEHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCCCCCEEEEE
MGWTGDYLDPLTFLESLFTTENQGFGAYGYSNKQYDNLIKQSNFAKDPLQRQEILRQAEA
ECCCCCCCCHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
IITEKDFPVAPLSILQSYYLFRHDKWTGWTPNVAEAYLYEEIKYKRENTNNKI
HHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKYKILIILSFLIVWLTSCSGDEHKNKITFRVTNEVEPDSLDPQLATSVQSFNIIMNIFS
CCEEEHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHC
GLTTRETQTGGYKPGLAKSWDISADGLVYTLHLREDLVWSDGVPITAEGIRKSYLRVLNK
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEECCCCCEEHHHHHHHHHHHHHH
ETASQYVDIVKSAIKNAHDYFEGKIPESELGIKAIDNSTLEITLIAPKPYFLDMLVHQTF
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCEEEECCCEEEEEEECCCHHHHHHHHHHHC
IPVPVHVIEKHGRNWTNPENIVTSGAYKLKTRIPNEKIVLEKNDKYYNASNVEIDEVIFY
CCCHHHHHHHCCCCCCCHHHHEECCCEEEEEECCCCEEEEECCCCEECCCCCEEEEEEEE
QVQGNTAYNMYVNDELDFLMNVASEYLDEARIRNDYYSHPINRVVYTAFNTTIKPLDNAK
EEECCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCEECCCCCHH
VREALTLAIDREALNKITLKGQSKPTRNLTPPFKNYSYDKKLKLFDPQRAKQLMAEAGYP
HHHHHHHHCCHHHHHEEEECCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCC
DGAGFPTLKFKTSQRNGMAISAEFLQEQLKKILNINIDIEIEEWNTFLSSRSTGNYQMSY
CCCCCCEEEEECCCCCCCEEEHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCCCCCEEEEE
MGWTGDYLDPLTFLESLFTTENQGFGAYGYSNKQYDNLIKQSNFAKDPLQRQEILRQAEA
ECCCCCCCCHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
IITEKDFPVAPLSILQSYYLFRHDKWTGWTPNVAEAYLYEEIKYKRENTNNKI
HHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2821267; 3525163; 11677609; 8202710; 8747465; 10422831; 10438628 [H]