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The map label for this gene is leuS

Identifier: 187918124

GI number: 187918124

Start: 258405

End: 260933

Strand: Direct

Name: leuS

Synonym: BH0251

Alternate gene names: 187918124

Gene position: 258405-260933 (Clockwise)

Preceding gene: 187918123

Following gene: 187918125

Centisome position: 28.02

GC content: 31.36

Gene sequence:

>2529_bases
ATGTCTGAATATAATTTCACTAAGATAGAAAAAAAATGGCAGGATTATTGGGATAAACACAAAACATACAAAGTTAATGA
AGATCCAAATATTCCTAAAGAAAAACGAATTTACATTCTTGATATGTTTCCCTACCCTTCAGCTAATGGACTCCATGTTG
GGCATCCTGAAGGTTATACAGCAACTGACATATTAACAAGATATAAACTCTTAAATGGATTTAATGTACTTCACCCAATA
GGATTTGATAGTTTTGGATTGCCTGCAGAAAATTACGCAATACAAACAGGAGAGCATCCAAAAAAAATAACAGAAAAAAA
TATTAAAAAATTTAAAGAACAAATTAAAGCATTAGGATTTGCCTATGATTGGGATAGAGAAATCAGAACCCACGATGAAA
ATTACTACAAATGGACACAATGGATTTTCCTAAAATTATATAAAAAAGGTTTAGCTTACACAAAAGAAATGCCCGTATGG
TACTGCCCTGACCTTGGAACAGTACTCTCAAATGAAGAAGTGATACAAACACCTGATGGACCTAGATCTGAGCGGGGATT
TCACAAAGTAAAAAGAAAACCTTTAAGACAATGGATACTCAAAATCACAGAATATGCAGAAAGACTAATTAAAGATCTCG
AAGAGATAGATTGGCCTGAATCTGTTAAAGAAATGCAAAAAAATTGGATTGGAAAGTCAACAGGCGCCGAGATCGAATTT
TCAGTAAAGGCAAGTAAAGAAAAGATAAAAGTGTTTACAACAAGACCAGACACAATTTTTGGAGTAACATATTTAGTGCT
TGCACCAGAGCATAGCATAGTAGATAAAATTACAAAAGATGAATTTAAACCTATGATCGTAGAGTATAGAGAAAGAGAAA
CTCTTAAAAGCGATCTTGAAAGAACTTCTCTTGAAAAAGATAAAACGGGAGTATTTACAGGCGCATATGCTATTAATCCA
ATAACTGGAGAAGAAATACCAATCTGGATAGGCAGCTATATACTTGGAACCTACGGCACTGGAGCTGTAATGAGTGTCCC
AGCACATGATGCTAGAGATTTTGAATTTGCAAAAAAATATAATCTACCAATCAAACAAGTAGTCTCTCAAACAGGAAACA
ATGAAATACTAACACAACCATTTACTGAAAATGGCATTTCAATTAATACCCCTGAAGAATTTAATAATCTTAAAACTGAT
GAAATCAAAGAAAGAGTAATTAAATGGCTTACTGAAAACAAGAAGGGTCAAAAAAAGGTTAATTATAAACTTAGAGATTG
GATTTTTTCAAGACAAAGATATTGGGGAGAACCCATTCCTATTATACTTGATGATGATTTAAATGAAATACCACTAGAAG
AAGATGAATTACCTCTAAGGCTTCCAGAAATAGAAAATTATAAACCATCAGGTACAGGCGAATCTCCTCTATCAAGAATC
CAAGATTGGGTCAATATTAAACGTAATGGAAAAACATATAAAAGAGAAACGAACACAATGCCTCAATGGGCAGGTTCCTG
CTGGTATTACATACGTTACCTTGATCCAAATAATGACAATGAATTTGCAAGTAAAGAAAAAATTAATTACTGGATGCCAG
TTGATCTTTATATTGGAGGTGCTGAGCACTCGGTATTACACTTACTATATGCAAGATTTTGGCATAAAGTTCTTTATGAT
TTAGGATATGTCAATACAAAAGAACCCTTTAAAAAACTAATAAATCAGGGAATGATAACATCATTTGCATATCAAGACGA
AAATGGTATTTTAATTCCCAATGACGAGGTTGAAAAGAAAGATAATAAGTTTTTTTCCAAAAAAAATAATAAGGAACTCA
AACAAATAGTTGCAAAAATGTCAAAATCACTCAAAAATATAATAAATCCAGACGATATTATTAAAGAATACGGTGCAGAT
TCAATGAGAATCTACGAAATGTTCATGGGCCCTTTAACTGATTCAAAACCTTGGAATACACAAGGACTAATTGGAATTTT
TAGATTCTTAAACAAAATATGGACTATTAAGAACAAAGAACTAACAAAAGAATCAGCATCAAAAGAAATAATATCTGAAC
TTCACAAAACAATCAAAAAAGTAACAGAAGACATAGAAAATTTAAATTTTAATACTGCAATTTCATCATTGATGATATTT
ATAAATGAACTTTTAAAACATGATAAAAATTACTTAGAAATCTTCAAGCCTCTAACCATTATCCTTTCACCATTTGCGCC
TCATCTAGGAGAAGAATTATGGGAATATATGGGAGAAACCCCCAGCATATTCAAAAGTGCAAAGTGGCCAGAATACGATC
CAAATCTAATTATTGATAATACAAGAGAGATTGTACTGCAAATTAATGGCAAAATAAAAGATAAAATTATCTTAAACAAA
GGCACAAATGAGGAAGCTCTTAAAAGTATTGCACTTAAAAATCACAAAATCATGCAAAATATACAAAATAAACAAATAAT
AAAGATCATTACGGTCAAAGATAAGCTTATAAATATAGTAACAAGATAA

Upstream 100 bases:

>100_bases
AAGTTAATAACAATATAAATATTAATTTACTTTTCAATGAAGTAACTATGAATATAAAAATGCTATTATAAACCTTAAAC
AATTATTTGCTGAGGGACAG

Downstream 100 bases:

>100_bases
CGAGGCAAAAATGGATTTGGAAACCTTAAGCATTAAATACAGAGTTTTTATATTAATAATATTCACATTAATAACAATTT
TTTTAGGATTTTTCTTAAAA

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS

Number of amino acids: Translated: 842; Mature: 841

Protein sequence:

>842_residues
MSEYNFTKIEKKWQDYWDKHKTYKVNEDPNIPKEKRIYILDMFPYPSANGLHVGHPEGYTATDILTRYKLLNGFNVLHPI
GFDSFGLPAENYAIQTGEHPKKITEKNIKKFKEQIKALGFAYDWDREIRTHDENYYKWTQWIFLKLYKKGLAYTKEMPVW
YCPDLGTVLSNEEVIQTPDGPRSERGFHKVKRKPLRQWILKITEYAERLIKDLEEIDWPESVKEMQKNWIGKSTGAEIEF
SVKASKEKIKVFTTRPDTIFGVTYLVLAPEHSIVDKITKDEFKPMIVEYRERETLKSDLERTSLEKDKTGVFTGAYAINP
ITGEEIPIWIGSYILGTYGTGAVMSVPAHDARDFEFAKKYNLPIKQVVSQTGNNEILTQPFTENGISINTPEEFNNLKTD
EIKERVIKWLTENKKGQKKVNYKLRDWIFSRQRYWGEPIPIILDDDLNEIPLEEDELPLRLPEIENYKPSGTGESPLSRI
QDWVNIKRNGKTYKRETNTMPQWAGSCWYYIRYLDPNNDNEFASKEKINYWMPVDLYIGGAEHSVLHLLYARFWHKVLYD
LGYVNTKEPFKKLINQGMITSFAYQDENGILIPNDEVEKKDNKFFSKKNNKELKQIVAKMSKSLKNIINPDDIIKEYGAD
SMRIYEMFMGPLTDSKPWNTQGLIGIFRFLNKIWTIKNKELTKESASKEIISELHKTIKKVTEDIENLNFNTAISSLMIF
INELLKHDKNYLEIFKPLTIILSPFAPHLGEELWEYMGETPSIFKSAKWPEYDPNLIIDNTREIVLQINGKIKDKIILNK
GTNEEALKSIALKNHKIMQNIQNKQIIKIITVKDKLINIVTR

Sequences:

>Translated_842_residues
MSEYNFTKIEKKWQDYWDKHKTYKVNEDPNIPKEKRIYILDMFPYPSANGLHVGHPEGYTATDILTRYKLLNGFNVLHPI
GFDSFGLPAENYAIQTGEHPKKITEKNIKKFKEQIKALGFAYDWDREIRTHDENYYKWTQWIFLKLYKKGLAYTKEMPVW
YCPDLGTVLSNEEVIQTPDGPRSERGFHKVKRKPLRQWILKITEYAERLIKDLEEIDWPESVKEMQKNWIGKSTGAEIEF
SVKASKEKIKVFTTRPDTIFGVTYLVLAPEHSIVDKITKDEFKPMIVEYRERETLKSDLERTSLEKDKTGVFTGAYAINP
ITGEEIPIWIGSYILGTYGTGAVMSVPAHDARDFEFAKKYNLPIKQVVSQTGNNEILTQPFTENGISINTPEEFNNLKTD
EIKERVIKWLTENKKGQKKVNYKLRDWIFSRQRYWGEPIPIILDDDLNEIPLEEDELPLRLPEIENYKPSGTGESPLSRI
QDWVNIKRNGKTYKRETNTMPQWAGSCWYYIRYLDPNNDNEFASKEKINYWMPVDLYIGGAEHSVLHLLYARFWHKVLYD
LGYVNTKEPFKKLINQGMITSFAYQDENGILIPNDEVEKKDNKFFSKKNNKELKQIVAKMSKSLKNIINPDDIIKEYGAD
SMRIYEMFMGPLTDSKPWNTQGLIGIFRFLNKIWTIKNKELTKESASKEIISELHKTIKKVTEDIENLNFNTAISSLMIF
INELLKHDKNYLEIFKPLTIILSPFAPHLGEELWEYMGETPSIFKSAKWPEYDPNLIIDNTREIVLQINGKIKDKIILNK
GTNEEALKSIALKNHKIMQNIQNKQIIKIITVKDKLINIVTR
>Mature_841_residues
SEYNFTKIEKKWQDYWDKHKTYKVNEDPNIPKEKRIYILDMFPYPSANGLHVGHPEGYTATDILTRYKLLNGFNVLHPIG
FDSFGLPAENYAIQTGEHPKKITEKNIKKFKEQIKALGFAYDWDREIRTHDENYYKWTQWIFLKLYKKGLAYTKEMPVWY
CPDLGTVLSNEEVIQTPDGPRSERGFHKVKRKPLRQWILKITEYAERLIKDLEEIDWPESVKEMQKNWIGKSTGAEIEFS
VKASKEKIKVFTTRPDTIFGVTYLVLAPEHSIVDKITKDEFKPMIVEYRERETLKSDLERTSLEKDKTGVFTGAYAINPI
TGEEIPIWIGSYILGTYGTGAVMSVPAHDARDFEFAKKYNLPIKQVVSQTGNNEILTQPFTENGISINTPEEFNNLKTDE
IKERVIKWLTENKKGQKKVNYKLRDWIFSRQRYWGEPIPIILDDDLNEIPLEEDELPLRLPEIENYKPSGTGESPLSRIQ
DWVNIKRNGKTYKRETNTMPQWAGSCWYYIRYLDPNNDNEFASKEKINYWMPVDLYIGGAEHSVLHLLYARFWHKVLYDL
GYVNTKEPFKKLINQGMITSFAYQDENGILIPNDEVEKKDNKFFSKKNNKELKQIVAKMSKSLKNIINPDDIIKEYGADS
MRIYEMFMGPLTDSKPWNTQGLIGIFRFLNKIWTIKNKELTKESASKEIISELHKTIKKVTEDIENLNFNTAISSLMIFI
NELLKHDKNYLEIFKPLTIILSPFAPHLGEELWEYMGETPSIFKSAKWPEYDPNLIIDNTREIVLQINGKIKDKIILNKG
TNEEALKSIALKNHKIMQNIQNKQIIKIITVKDKLINIVTR

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7661872, Length=884, Percent_Identity=30.9954751131222, Blast_Score=437, Evalue=1e-122,
Organism=Escherichia coli, GI1786861, Length=873, Percent_Identity=39.7479954180985, Blast_Score=610, Evalue=1e-175,
Organism=Escherichia coli, GI1790708, Length=966, Percent_Identity=20.9109730848861, Blast_Score=103, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71997510, Length=830, Percent_Identity=30.3614457831325, Blast_Score=315, Evalue=6e-86,
Organism=Caenorhabditis elegans, GI71997517, Length=781, Percent_Identity=30.729833546735, Blast_Score=315, Evalue=7e-86,
Organism=Caenorhabditis elegans, GI212645227, Length=327, Percent_Identity=30.8868501529052, Blast_Score=145, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6323414, Length=813, Percent_Identity=37.3923739237392, Blast_Score=469, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6321531, Length=512, Percent_Identity=21.6796875, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21355409, Length=839, Percent_Identity=30.8700834326579, Blast_Score=374, Evalue=1e-103,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYL_BORHD (B2RZW1)

Other databases:

- EMBL:   CP000048
- RefSeq:   YP_001883687.1
- EnsemblBacteria:   EBBORT00000006763
- GeneID:   6277013
- GenomeReviews:   CP000048_GR
- KEGG:   bhr:BH0251
- GeneTree:   EBGT00050000007023
- HOGENOM:   HBG693845
- OMA:   ERGNFPV
- ProtClustDB:   PRK00390
- BioCyc:   BHER314723:BH0251-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00049_B
- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11946:SF7
- PRINTS:   PR00985
- TIGRFAMs:   TIGR00396

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1; SSF47323 tRNAsyn_1a_bind; SSF50677 ValRS_IleRS_edit

EC number: =6.1.1.4

Molecular weight: Translated: 98522; Mature: 98391

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 622-622

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEYNFTKIEKKWQDYWDKHKTYKVNEDPNIPKEKRIYILDMFPYPSANGLHVGHPEGYT
CCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEECCCCCCCCEECCCCCCCH
ATDILTRYKLLNGFNVLHPIGFDSFGLPAENYAIQTGEHPKKITEKNIKKFKEQIKALGF
HHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCC
AYDWDREIRTHDENYYKWTQWIFLKLYKKGLAYTKEMPVWYCPDLGTVLSNEEVIQTPDG
CCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHCCCCEEECCCC
PRSERGFHKVKRKPLRQWILKITEYAERLIKDLEEIDWPESVKEMQKNWIGKSTGAEIEF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEE
SVKASKEKIKVFTTRPDTIFGVTYLVLAPEHSIVDKITKDEFKPMIVEYRERETLKSDLE
EEECCCCEEEEEECCCCHHHCEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
RTSLEKDKTGVFTGAYAINPITGEEIPIWIGSYILGTYGTGAVMSVPAHDARDFEFAKKY
HHHCCCCCCCEEECCEEECCCCCCCCCEEHHHHHHHCCCCCCEEECCCCCCCCHHHHHHC
NLPIKQVVSQTGNNEILTQPFTENGISINTPEEFNNLKTDEIKERVIKWLTENKKGQKKV
CCCHHHHHHCCCCCCEEECCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCHHH
NYKLRDWIFSRQRYWGEPIPIILDDDLNEIPLEEDELPLRLPEIENYKPSGTGESPLSRI
CHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHH
QDWVNIKRNGKTYKRETNTMPQWAGSCWYYIRYLDPNNDNEFASKEKINYWMPVDLYIGG
HHHHHHHCCCCCHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHCCCEEEEEEEEECC
AEHSVLHLLYARFWHKVLYDLGYVNTKEPFKKLINQGMITSFAYQDENGILIPNDEVEKK
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCEECCCCHHHHH
DNKFFSKKNNKELKQIVAKMSKSLKNIINPDDIIKEYGADSMRIYEMFMGPLTDSKPWNT
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCC
QGLIGIFRFLNKIWTIKNKELTKESASKEIISELHKTIKKVTEDIENLNFNTAISSLMIF
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
INELLKHDKNYLEIFKPLTIILSPFAPHLGEELWEYMGETPSIFKSAKWPEYDPNLIIDN
HHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEC
TREIVLQINGKIKDKIILNKGTNEEALKSIALKNHKIMQNIQNKQIIKIITVKDKLINIV
CEEEEEEECCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHH
TR
CC
>Mature Secondary Structure 
SEYNFTKIEKKWQDYWDKHKTYKVNEDPNIPKEKRIYILDMFPYPSANGLHVGHPEGYT
CCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEECCCCCCCCEECCCCCCCH
ATDILTRYKLLNGFNVLHPIGFDSFGLPAENYAIQTGEHPKKITEKNIKKFKEQIKALGF
HHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCC
AYDWDREIRTHDENYYKWTQWIFLKLYKKGLAYTKEMPVWYCPDLGTVLSNEEVIQTPDG
CCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHCCCCEEECCCC
PRSERGFHKVKRKPLRQWILKITEYAERLIKDLEEIDWPESVKEMQKNWIGKSTGAEIEF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEE
SVKASKEKIKVFTTRPDTIFGVTYLVLAPEHSIVDKITKDEFKPMIVEYRERETLKSDLE
EEECCCCEEEEEECCCCHHHCEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
RTSLEKDKTGVFTGAYAINPITGEEIPIWIGSYILGTYGTGAVMSVPAHDARDFEFAKKY
HHHCCCCCCCEEECCEEECCCCCCCCCEEHHHHHHHCCCCCCEEECCCCCCCCHHHHHHC
NLPIKQVVSQTGNNEILTQPFTENGISINTPEEFNNLKTDEIKERVIKWLTENKKGQKKV
CCCHHHHHHCCCCCCEEECCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCHHH
NYKLRDWIFSRQRYWGEPIPIILDDDLNEIPLEEDELPLRLPEIENYKPSGTGESPLSRI
CHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHH
QDWVNIKRNGKTYKRETNTMPQWAGSCWYYIRYLDPNNDNEFASKEKINYWMPVDLYIGG
HHHHHHHCCCCCHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHCCCEEEEEEEEECC
AEHSVLHLLYARFWHKVLYDLGYVNTKEPFKKLINQGMITSFAYQDENGILIPNDEVEKK
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCEECCCCHHHHH
DNKFFSKKNNKELKQIVAKMSKSLKNIINPDDIIKEYGADSMRIYEMFMGPLTDSKPWNT
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCC
QGLIGIFRFLNKIWTIKNKELTKESASKEIISELHKTIKKVTEDIENLNFNTAISSLMIF
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
INELLKHDKNYLEIFKPLTIILSPFAPHLGEELWEYMGETPSIFKSAKWPEYDPNLIIDN
HHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEC
TREIVLQINGKIKDKIILNKGTNEEALKSIALKNHKIMQNIQNKQIIKIITVKDKLINIV
CEEEEEEECCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHH
TR
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA