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The map label for this gene is dus2 [H]

Identifier: 187918097

GI number: 187918097

Start: 228384

End: 229391

Strand: Direct

Name: dus2 [H]

Synonym: BH0225

Alternate gene names: 187918097

Gene position: 228384-229391 (Clockwise)

Preceding gene: 187918091

Following gene: 187918099

Centisome position: 24.76

GC content: 29.86

Gene sequence:

>1008_bases
ATGTTAATAAACCGAAAAATATCGGTAGCACCAATGGTAGCAATTACCGATGAACACTTTAGATATATGATAAGATTATT
ATCAAAAAAAGTCACTTTATACACTCCAATGATTTCTGCAAAATCAATTATCATGGGCAATTTAAATAAAGTTGTAAAAC
AAACTCCCACTGATTCTCCAATTGCAATTCAAATAGCAACAAGCTGTGAGAATGATGCTGCAAAAGCTATAGAAATTCTT
GAAAATAAATTTAATTTTGATGAATATAATCTTAACGTTGGCTGCCCATCATCTAAGGTCCAAAACGCAAACTATGGGGC
CTGTCTAATGCAAACCCCAACCCAAGTAGGCAAAATCTTGAAAGCCATGAAAAAAAATACAAATAAACCTGTCTCAATCA
AACACAGAATAGGTATAAGAAATAATAAAAGAGAATATCATGAAGAAAACTACACAGAACTGCAACAATTCGTAGAAAAA
ATTATGGAATATGAAATAAAAAATTTTACTGTTCATGCACGAATAGCCATATTAAATGGATACTCTCCTAAAGATAATCA
AAATATCCCAAAACTTAGACATGACTTTGTATATCGATTGAAACAAGAACATAAAAATATATTTATTGAAGTAAATGGAG
GAATTAAGAGTAGCGATCACATAAAAACACATCTATCTTATGTAGATTCCGTCATGATTGGAAGAGCTGTAGCAAAGGAT
CCATATTTAATTGCAAAAATTTCAAGAGAATTCCTAGAAGAAAAGGAAAAAATTCCAACAAGAGAAGAAATATTATTAAA
AATGGTAGAATATATCAAAGAGTACGATGAATGCCTTTCAATTAGCACTATACTAAAACACATAATGAGCATAGTATTTG
CCAAAGAAAATGCTTGTAAATTTAGACAAACTTTAAGTGCACCTTTTCCTAAAAATCTTAAAAATCACGAAATACTCTTA
AGTGCAATTGAACACTTAAGAGAAGATACTTTAAAATCTAATTCTTAG

Upstream 100 bases:

>100_bases
CTTTTTACCAAAATAAATCCTTACCTCCCGGTTTTTATAAATTTTTTTTAAAAAATTATAATATTGTCAAATTAAACAAT
TTATTTTAGCATATTATGCT

Downstream 100 bases:

>100_bases
GGGATATATAATCAAAACCTGTATATTTAACCAAATTCTTAGGTATTCTAACTCCGCCTCTTTCATCCTGAAAGTTCTCA
AGTATAGCAATAATAGTTCT

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MLINRKISVAPMVAITDEHFRYMIRLLSKKVTLYTPMISAKSIIMGNLNKVVKQTPTDSPIAIQIATSCENDAAKAIEIL
ENKFNFDEYNLNVGCPSSKVQNANYGACLMQTPTQVGKILKAMKKNTNKPVSIKHRIGIRNNKREYHEENYTELQQFVEK
IMEYEIKNFTVHARIAILNGYSPKDNQNIPKLRHDFVYRLKQEHKNIFIEVNGGIKSSDHIKTHLSYVDSVMIGRAVAKD
PYLIAKISREFLEEKEKIPTREEILLKMVEYIKEYDECLSISTILKHIMSIVFAKENACKFRQTLSAPFPKNLKNHEILL
SAIEHLREDTLKSNS

Sequences:

>Translated_335_residues
MLINRKISVAPMVAITDEHFRYMIRLLSKKVTLYTPMISAKSIIMGNLNKVVKQTPTDSPIAIQIATSCENDAAKAIEIL
ENKFNFDEYNLNVGCPSSKVQNANYGACLMQTPTQVGKILKAMKKNTNKPVSIKHRIGIRNNKREYHEENYTELQQFVEK
IMEYEIKNFTVHARIAILNGYSPKDNQNIPKLRHDFVYRLKQEHKNIFIEVNGGIKSSDHIKTHLSYVDSVMIGRAVAKD
PYLIAKISREFLEEKEKIPTREEILLKMVEYIKEYDECLSISTILKHIMSIVFAKENACKFRQTLSAPFPKNLKNHEILL
SAIEHLREDTLKSNS
>Mature_335_residues
MLINRKISVAPMVAITDEHFRYMIRLLSKKVTLYTPMISAKSIIMGNLNKVVKQTPTDSPIAIQIATSCENDAAKAIEIL
ENKFNFDEYNLNVGCPSSKVQNANYGACLMQTPTQVGKILKAMKKNTNKPVSIKHRIGIRNNKREYHEENYTELQQFVEK
IMEYEIKNFTVHARIAILNGYSPKDNQNIPKLRHDFVYRLKQEHKNIFIEVNGGIKSSDHIKTHLSYVDSVMIGRAVAKD
PYLIAKISREFLEEKEKIPTREEILLKMVEYIKEYDECLSISTILKHIMSIVFAKENACKFRQTLSAPFPKNLKNHEILL
SAIEHLREDTLKSNS

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Escherichia coli, GI145693211, Length=303, Percent_Identity=34.983498349835, Blast_Score=197, Evalue=8e-52,
Organism=Escherichia coli, GI1789660, Length=265, Percent_Identity=27.9245283018868, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 38557; Mature: 38557

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLINRKISVAPMVAITDEHFRYMIRLLSKKVTLYTPMISAKSIIMGNLNKVVKQTPTDSP
CCCCCCCCCCEEEEECHHHHHHHHHHHHCCHHEEECCHHHHHHHHHHHHHHHHCCCCCCC
IAIQIATSCENDAAKAIEILENKFNFDEYNLNVGCPSSKVQNANYGACLMQTPTQVGKIL
EEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHCCCCCCCEEEECCHHHHHHHH
KAMKKNTNKPVSIKHRIGIRNNKREYHEENYTELQQFVEKIMEYEIKNFTVHARIAILNG
HHHHHCCCCCEEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCEEEEEEEEEEEC
YSPKDNQNIPKLRHDFVYRLKQEHKNIFIEVNGGIKSSDHIKTHLSYVDSVMIGRAVAKD
CCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PYLIAKISREFLEEKEKIPTREEILLKMVEYIKEYDECLSISTILKHIMSIVFAKENACK
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
FRQTLSAPFPKNLKNHEILLSAIEHLREDTLKSNS
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLINRKISVAPMVAITDEHFRYMIRLLSKKVTLYTPMISAKSIIMGNLNKVVKQTPTDSP
CCCCCCCCCCEEEEECHHHHHHHHHHHHCCHHEEECCHHHHHHHHHHHHHHHHCCCCCCC
IAIQIATSCENDAAKAIEILENKFNFDEYNLNVGCPSSKVQNANYGACLMQTPTQVGKIL
EEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHCCCCCCCEEEECCHHHHHHHH
KAMKKNTNKPVSIKHRIGIRNNKREYHEENYTELQQFVEKIMEYEIKNFTVHARIAILNG
HHHHHCCCCCEEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCEEEEEEEEEEEC
YSPKDNQNIPKLRHDFVYRLKQEHKNIFIEVNGGIKSSDHIKTHLSYVDSVMIGRAVAKD
CCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PYLIAKISREFLEEKEKIPTREEILLKMVEYIKEYDECLSISTILKHIMSIVFAKENACK
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
FRQTLSAPFPKNLKNHEILLSAIEHLREDTLKSNS
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]