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The map label for this gene is dacD

Identifier: 187733446

GI number: 187733446

Start: 1130552

End: 1130770

Strand: Direct

Name: dacD

Synonym: SbBS512_E1225

Alternate gene names: 187733446

Gene position: 1130552-1130770 (Clockwise)

Preceding gene: 187730226

Following gene: 187731583

Centisome position: 24.49

GC content: 50.23

Gene sequence:

>219_bases
ATGGTGCTACCGAAAGCCGAAATTCCACATATCAAAGCCAAATATACCCTTGATGGTAAAGAACTCACCGCGCCAATTAG
CGCCCATCAGCGGGTAGGGGAAATTGAACTTTACGACCGTGATAAACAGGTGGCGCACTGGCCGCTGGTTACCCTGGAAT
CTGTCGGGGAAGGCAGCATGTTTTCTCGCCTGAGTGATTATTTCCACCATAAGGCCTGA

Upstream 100 bases:

>100_bases
ACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCA
GCATCACCCAAGAGTTCTGG

Downstream 100 bases:

>100_bases
CCTTTCTTTTGCAGCAGACTGGCAGGAGTGCGAGTCTGCTCGCATAATCAACACTCATTCCTTGTGGTTTTAATTTTGCA
ACTATACTGTATATAAAAAC

Product: penicillin-binding protein 6B

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 6b; PBP-6b

Number of amino acids: Translated: 72; Mature: 72

Protein sequence:

>72_residues
MVLPKAEIPHIKAKYTLDGKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA

Sequences:

>Translated_72_residues
MVLPKAEIPHIKAKYTLDGKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA
>Mature_72_residues
MVLPKAEIPHIKAKYTLDGKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Note=N-terminal lies in the periplasmic space (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family

Homologues:

Organism=Escherichia coli, GI87082022, Length=72, Percent_Identity=100, Blast_Score=152, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DACD_ECOLI (P33013)

Other databases:

- EMBL:   U00009
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   A64966
- RefSeq:   AP_002610.1
- RefSeq:   NP_416514.4
- ProteinModelPortal:   P33013
- SMR:   P33013
- STRING:   P33013
- MEROPS:   S11.009
- EnsemblBacteria:   EBESCT00000000983
- EnsemblBacteria:   EBESCT00000014692
- GeneID:   946518
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5329
- KEGG:   eco:b2010
- EchoBASE:   EB1839
- EcoGene:   EG11893
- eggNOG:   COG1686
- GeneTree:   EBGT00050000011015
- HOGENOM:   HBG728991
- OMA:   WGLQNFA
- ProtClustDB:   PRK11397
- BioCyc:   EcoCyc:RPOA-MONOMER
- Genevestigator:   P33013
- GO:   GO:0006508
- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967
- Gene3D:   G3DSA:2.60.410.10
- PRINTS:   PR00725
- SMART:   SM00936

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11; SSF56601 PBP_transp_fold; SSF69189 Peniciliin-bd_prot-assoc

EC number: =3.4.16.4

Molecular weight: Translated: 8187; Mature: 8187

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: ACT_SITE 63-63 ACT_SITE 66-66 ACT_SITE 129-129 BINDING 232-232

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLPKAEIPHIKAKYTLDGKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSM
CCCCCCCCCCEEEEEEECCCCEECCCCCCCCCCCEEEECCCCCCCCCCEEEECCCCCCHH
FSRLSDYFHHKA
HHHHHHHHHCCC
>Mature Secondary Structure
MVLPKAEIPHIKAKYTLDGKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSM
CCCCCCCCCCEEEEEEECCCCEECCCCCCCCCCCEEEECCCCCCCCCCEEEECCCCCCHH
FSRLSDYFHHKA
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8955390; 9097040; 9278503