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The map label for this gene is ntpA

Identifier: 187731919

GI number: 187731919

Start: 1007722

End: 1008174

Strand: Direct

Name: ntpA

Synonym: SbBS512_E1107

Alternate gene names: 187731919

Gene position: 1007722-1008174 (Clockwise)

Preceding gene: 187733090

Following gene: 187731444

Centisome position: 21.83

GC content: 52.1

Gene sequence:

>453_bases
GTGAAGGATAAAGTGTATAAGCGTCCCGTTTCGATCTTAGTGGTCATCTATGCACAAGATACGAAACGGGTGCTGATGTT
GCAGCGGCGTGACGATCCCGATTTCTGGCAGTCGGTAACCGGCAGCGTGGAAGAGGGTGAAACCGCGCCGCAAGCTGCCA
TGCGCGAAGTAAAGGAAGAGGTCACCATTGATGTTGTCGCTGAACAACTGACCTTAATTGACTGTCAGCGCACGGTAGAG
TTTGAAATTTTTTCACATTTACGTCATCGCTATGCGCCGGGCGTGACGCGTAATACGGAATCATGGTTCTGTCTTGCGCT
TCCGCATGAGCGGCAGATCGTTTTCACTGAACATCTGGCTTACAAGTGGCTTGATGCGCCTGCTGCGGCGGCGCTCACTA
AGTCCTGGAGCAACCGGCAGGCGATTGAACAGTTTGTAATTAACGCTGCCTGA

Upstream 100 bases:

>100_bases
AAATCATTCGCGTTGCATCAAGGCGGCAATTGAGTGAACTCCCATGAGCATAGATAACTATGTGAATGGGATGAGCGAAG
GCAGTCAACGAAGAGGCAGC

Downstream 100 bases:

>100_bases
ACAGGCAGGGCTATCTGGAGATATTTTTATGGCAGGTCATAGTAAATGGGCCAACACCAGACATCGTAAAGCTGCGCAGG
ATGCTAAGCGCGGTAAAATC

Product: dATP pyrophosphohydrolase

Products: dAMP; pyrophosphate [C]

Alternate protein names: dATP pyrophosphohydrolase

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVEEGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVE
FEIFSHLRHRYAPGVTRNTESWFCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA

Sequences:

>Translated_150_residues
MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVEEGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVE
FEIFSHLRHRYAPGVTRNTESWFCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA
>Mature_150_residues
MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVEEGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVE
FEIFSHLRHRYAPGVTRNTESWFCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA

Specific function: Catalyzes the hydrolysis of dihydroneopterin triphosphate to dihydroneopterin monophosphate and pyrophosphate. Required for efficient folate biosynthesis. Can also hydrolyze nucleoside triphosphates with a preference for dATP

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain

Homologues:

Organism=Escherichia coli, GI1788172, Length=150, Percent_Identity=100, Blast_Score=312, Evalue=7e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUDB_ECO57 (P0AFC1)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C85799
- PIR:   G90950
- RefSeq:   NP_288302.1
- RefSeq:   NP_310602.1
- ProteinModelPortal:   P0AFC1
- SMR:   P0AFC1
- EnsemblBacteria:   EBESCT00000027486
- EnsemblBacteria:   EBESCT00000057343
- GeneID:   914189
- GeneID:   961838
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2917
- KEGG:   ecs:ECs2575
- GeneTree:   EBGT00050000011590
- HOGENOM:   HBG495074
- OMA:   PGFWQSV
- ProtClustDB:   PRK09438
- BioCyc:   ECOL83334:ECS2575-MONOMER
- InterPro:   IPR003564
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797
- Gene3D:   G3DSA:3.90.79.10
- PRINTS:   PR01404

Pfam domain/function: PF00293 NUDIX; SSF55811 NUDIX_hydrolase

EC number: 3.6.1.- [C]

Molecular weight: Translated: 17306; Mature: 17306

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS51462 NUDIX; PS00893 NUDIX_BOX

Important sites: BINDING 7-7 BINDING 29-29 BINDING 40-40 BINDING 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVEEGETAPQAAMREVKEE
CCCCHHHCCEEEEEEEEECCCCEEEEEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHH
VTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESWFCLALPHERQIVFTEHLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEEEEHHHHH
YKWLDAPAAAALTKSWSNRQAIEQFVINAA
HHHHCCHHHHHHHHCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVEEGETAPQAAMREVKEE
CCCCHHHCCEEEEEEEEECCCCEEEEEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHH
VTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESWFCLALPHERQIVFTEHLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEEEEHHHHH
YKWLDAPAAAALTKSWSNRQAIEQFVINAA
HHHHCCHHHHHHHHCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxy-ATP; H2O [C]

Specific reaction: deoxy-ATP + H2O = dAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796