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The map label for this gene is wzb

Identifier: 187730901

GI number: 187730901

Start: 1073991

End: 1074434

Strand: Direct

Name: wzb

Synonym: SbBS512_E1171

Alternate gene names: 187730901

Gene position: 1073991-1074434 (Clockwise)

Preceding gene: 187731528

Following gene: 187731566

Centisome position: 23.27

GC content: 54.28

Gene sequence:

>444_bases
ATGTTTAACAACATCTTAGTTGTCTGTGTCGGCAATATTTGCCGTTCCCCGACGGCGGAACGCTTACTGCAACGTTATCA
CCCGGAGCTGAAAGTGGAGTCCGCTGGACTCGGCGCGCTGGTCGGTAAGGGCGCTGATCCTACCGCTATCAGCGTCGCCG
CAGAACATCAACTGTCTCTGGAAGGTCACTGTGCACGGCAAATCAGCCGCCGCCTGTGTCGCAACTATGACCTGATTTTG
ACCATGGAAAAGCGCCATATCGAACGCTTATGCGAGATGGCACCTGAGATGCGCGGCAAAGTGATGCTGTTTGGTCACTG
GGATAACGAATGTGAAATCCCCGATCCGTATCGCAAAAGCCGGGAAACGTTTGCAGCGGTGTACACATTACTTGAACGGT
CTGCCCGCCAGTGGGCGCAGGCATTGAACGCAGAGCAGGTATAA

Upstream 100 bases:

>100_bases
TTTCCCGCTGGAACCGTCTGATCAATCAGTTGCTGCCAACTATTAGCGGTGTCCGTTACATGACGGATACAGCCAGCGAC
ATTCATAACTGGTAATCGTC

Downstream 100 bases:

>100_bases
GAATGACAGAAAAAGTAAAACAACATGCCGCTCCGGTAACGGGCAGTGATGAAATCGATATTGGTCGCCTGGTCGGCACC
GTCATTGAAGCGCGCTGGTG

Product: tyrosine phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKGADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLIL
TMEKRHIERLCEMAPEMRGKVMLFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV

Sequences:

>Translated_147_residues
MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKGADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLIL
TMEKRHIERLCEMAPEMRGKVMLFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV
>Mature_147_residues
MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKGADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLIL
TMEKRHIERLCEMAPEMRGKVMLFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV

Specific function: Dephosphorylates wzc. Required for the extracellular polysaccharide colanic acid synthesis. Probably involved in the export of colanic acid from the cell to medium

COG id: COG0394

COG function: function code T; Protein-tyrosine-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the low molecular weight phosphotyrosine protein phosphatase family

Homologues:

Organism=Homo sapiens, GI6005988, Length=147, Percent_Identity=30.6122448979592, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI1788375, Length=147, Percent_Identity=100, Blast_Score=306, Evalue=2e-85,
Organism=Escherichia coli, GI87081812, Length=142, Percent_Identity=56.3380281690141, Blast_Score=159, Evalue=8e-41,
Organism=Drosophila melanogaster, GI78706766, Length=139, Percent_Identity=32.3741007194245, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI78706768, Length=139, Percent_Identity=32.3741007194245, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI78706764, Length=127, Percent_Identity=33.0708661417323, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): WZB_ECO57 (P0AAB3)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B90987
- PIR:   E85832
- RefSeq:   NP_288567.1
- RefSeq:   NP_310893.1
- ProteinModelPortal:   P0AAB3
- SMR:   P0AAB3
- EnsemblBacteria:   EBESCT00000027437
- EnsemblBacteria:   EBESCT00000058441
- GeneID:   913690
- GeneID:   956740
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3226
- KEGG:   ecs:ECs2866
- GeneTree:   EBGT00050000010250
- HOGENOM:   HBG730979
- OMA:   ENSRQIL
- ProtClustDB:   PRK10126
- BioCyc:   ECOL83334:ECS2866-MONOMER
- InterPro:   IPR000106
- InterPro:   IPR017867
- PANTHER:   PTHR11717
- PRINTS:   PR00719
- SMART:   SM00226

Pfam domain/function: PF01451 LMWPc; SSF52788 Tyr_Pase_low_mol_wt

EC number: =3.1.3.48

Molecular weight: Translated: 16710; Mature: 16710

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: ACT_SITE 9-9 ACT_SITE 14-14 ACT_SITE 115-115

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKGADPTAISVAAEHQLSL
CCCCEEEEEECHHHCCCHHHHHHHHHCCCCEECCCCCCHHHCCCCCCCEEEEEHHCCCCC
EGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVMLFGHWDNECEIPDPYRKS
CHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHHH
RETFAAVYTLLERSARQWAQALNAEQV
HHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKGADPTAISVAAEHQLSL
CCCCEEEEEECHHHCCCHHHHHHHHHCCCCEECCCCCCHHHCCCCCCCEEEEEHHCCCCC
EGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVMLFGHWDNECEIPDPYRKS
CHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHHH
RETFAAVYTLLERSARQWAQALNAEQV
HHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796